Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,266,916	G→C	6.8%	R360G (CGC→GGC)	quiP ←	acyl‑homoserine lactone acylase QuiP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,266,916	0	G	C	6.8%	178.0 / 4.8	59	R360G (CGC→GGC)	quiP	acyl‑homoserine lactone acylase QuiP
Reads supporting (aligned to +/- strand):  ref base G (28/27);  new base C (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

ATCCAGGCTGCGATCACCTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACAATTG  >  NC_002947/1266767‑1267065                                                                                                                                                      |                                                                                                                                                       aTCCAGGCTGCGATCACCTATGAACTAGGGCAGGGTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGCGCCGTGGGGGGTGGCCTACATCACTTAGTGCACGGAGCGATGGCGGCGTAAACAGTAGGTTCCGCTgcg                                                                                                                                                        >  2:598322/1‑150 (MQ=255)        cTGCGATCACCTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTgcgggcgcgg                                                                                                                                                 <  2:868178/150‑1 (MQ=255)                 ccTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGc                                                                                                                                        <  1:90108/150‑1 (MQ=255)                  cTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGcc                                                                                                                                       >  1:1841826/1‑150 (MQ=255)                   tttGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTCGTTGACTTCGCGCACGGGGCGTTGGCCACGTCCACAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCa                                                                                                                                      >  1:1278260/1‑150 (MQ=255)                   tttGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGGCGTCGGCCa                                                                                                                                      >  1:1514401/1‑150 (MQ=255)                          tGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCCGGCCCGCGCCCGCAGcgaaa                                                                                                                                    <  2:878625/145‑5 (MQ=255)                          tGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCCGGCCCGCGCCCGCAGcgaaa                                                                                                                                    >  1:878625/1‑141 (MQ=255)                             ggCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCg                                                                                                                            <  1:1598401/150‑1 (MQ=255)                              gCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCgg                                                                                                                           >  1:446643/1‑150 (MQ=255)                                  gTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAg                                                                                                                       >  1:852809/1‑150 (MQ=255)                                  gTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAg                                                                                                                       <  1:957333/150‑1 (MQ=255)                                     cAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTa                                                                                                                    >  1:906125/1‑150 (MQ=255)                                          ccaggcccaggcTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCa                                                                                                              <  2:1841826/150‑1 (MQ=255)                                            aggcccaggcTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGc                                                                                                            <  1:997417/150‑1 (MQ=255)                                            aggcccaggcTGGCGTTTTCTGGCTGGGAGAAGAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGc                                                                                                            >  1:527647/1‑150 (MQ=255)                                                      tGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCtt                                                                                                  >  1:1098824/1‑150 (MQ=255)                                                       ggCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTg                                                                                                 <  2:1892114/150‑1 (MQ=255)                                                           ttttCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTggcgg                                                                                             <  1:936305/150‑1 (MQ=255)                                                                 ggctggGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGTCCGGCAGCCATTTCCCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAgc                                                                                       <  1:1504952/150‑1 (MQ=255)                                                                 ggctggGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAgc                                                                                       <  1:177801/150‑1 (MQ=255)                                                                  gctggGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCTATTTACGATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAgct                                                                                      >  2:850454/1‑150 (MQ=255)                                                                  gctggGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGGTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGATGACTGCCTTGGCGGCGCAgct                                                                                      >  1:97978/1‑150 (MQ=255)                                                                     gggCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAgctgct                                                                                   <  2:771584/150‑1 (MQ=255)                                                                        ccAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCCGGCCCGCGcc                                                                                                                                              <  2:1654254/89‑1 (MQ=255)                                                                          agcagGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAgg                                                                              >  1:1090969/1‑150 (MQ=255)                                                                          agcagGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAgg                                                                              >  1:1253788/1‑150 (MQ=255)                                                                                 tGCCGTGGCGGGTCTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGt                                                                       <  2:204879/150‑1 (MQ=255)                                                                                      gggCGGGTGTCGTACATCACTTCGCGCACGGGAGGCGAGGCAGGTACAAAGTAGGTCTCGCTGCGGGCGCGGGCCGGCAGCGATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGa                                                                  <  2:1095359/149‑1 (MQ=255)                                                                                          gggTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAg                                                                                                                   <  1:115474/98‑1 (MQ=255)                                                                                          gggTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAg                                                                                                                   >  2:115474/1‑98 (MQ=255)                                                                                              gtCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGc                                                          <  1:1122210/150‑1 (MQ=255)                                                                                               tCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGcc                                                         >  2:1446281/1‑150 (MQ=255)                                                                                                      tCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAg                                                                         <  1:1221867/127‑1 (MQ=255)                                                                                                      tCACTTCGCGCAAGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAg                                                                         >  2:1221867/1‑127 (MQ=255)                                                                                                          ttCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCgct                                              >  1:852234/1‑150 (MQ=255)                                                                                                          ttCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGCCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGGCCTGATTGTCGGCCATGACCGCgct                                              >  2:950062/1‑150 (MQ=255)                                                                                                            cgcgCACGGGGCGTTGGCCACTTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTGAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCgctgc                                            <  2:619196/150‑1 (MQ=255)                                                                                                                 aCGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTc                                       <  2:569864/150‑1 (MQ=255)                                                                                                                    cggCCTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCGTCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCCAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAg                                    <  1:598322/149‑1 (MQ=255)                                                                                                                       cGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGcc                                 <  2:1228928/150‑1 (MQ=255)                                                                                                                        gTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGa                                                                  >  2:891517/1‑116 (MQ=255)                                                                                                                        gTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGa                                                                  <  1:891517/116‑1 (MQ=255)                                                                                                                            gCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGAGAGTCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCtt                            <  1:310819/150‑1 (MQ=255)                                                                                                                               aCGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGcc                         >  1:1689769/1‑150 (MQ=255)                                                                                                                                      aaGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCCTCGGCCAGGTAG‑CTCCGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAg                  <  2:1404432/150‑1 (MQ=255)                                                                                                                                        gTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTa                   >  2:991889/1‑147 (MQ=255)                                                                                                                                        gTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTa                   <  1:991889/147‑1 (MQ=255)                                                                                                                                        gTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGcc                <  2:852234/150‑1 (MQ=255)                                                                                                                                         tAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCg               >  1:742512/1‑150 (MQ=255)                                                                                                                                         tAGGTTTCGCGGCGGGCGCGGGCCGGCAGCCATTTACAATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCg               <  2:308958/150‑1 (MQ=255)                                                                                                                                            gTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCa                                                                                                                        <  1:1364796/43‑1 (MQ=255)                                                                                                                                            gTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCa                                                                                                                        >  2:1364796/1‑43 (MQ=255)                                                                                                                                            gTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCa                        <  2:335575/138‑1 (MQ=255)                                                                                                                                            gTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCa                        >  1:335575/1‑138 (MQ=255)                                                                                                                                            gTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGcc            >  1:1752307/1‑150 (MQ=255)                                                                                                                                            gTTTCGCTCCGAGAACGTGACAGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGcc            >  2:78970/1‑150 (MQ=255)                                                                                                                                             gttCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCCGCCAGATAG‑CTACCAAGACTGCCTTGGCGGCTCGGCTGCTCGAGGAACAGGTCCTGATTTTCGGCCAGGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCa           >  1:1469882/2‑150 (MQ=255)                                                                                                                                               tggCTGCCGGCCCGCGCCCGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTTCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGc         >  1:100045/3‑150 (MQ=255)                                                                                                                                                 gCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAAGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCATCTTGCCATTGTAGCCGGCCAGcac       >  2:905577/1‑150 (MQ=255)                                                                                                                                                   tGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGATGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACaa     >  2:1499085/1‑150 (MQ=255)                                                                                                                                                      gggcgcgggcCGGCAGCCATTTACCATCGGCCAGGTAGACACCATTAATAGTATTCG‑GGGGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACAATTg  <  2:527667/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                       ATCCAGGCTGCGATCACCTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACAATTG  >  NC_002947/1266767‑1267065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑