Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,266,923 G→C 6.9% A357A (GCC→GCG)  quiP ← acyl‑homoserine lactone acylase QuiP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,266,9230GC 6.9% 168.3 / 3.6 59A357A (GCC→GCG) quiPacyl‑homoserine lactone acylase QuiP
Reads supporting (aligned to +/- strand):  ref base G (27/27);  new base C (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

CTGCGATCACCTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACAATTGGCAG  >  NC_002947/1266774‑1267069
                                                                                                                                                     |                                                                                                                                                   
cTGCGATCACCTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTgcgggcgcgg                                                                                                                                                     <  2:868178/150‑1 (MQ=255)
         ccTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGc                                                                                                                                            <  1:90108/150‑1 (MQ=255)
          cTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGcc                                                                                                                                           >  1:1841826/1‑150 (MQ=255)
           tttGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTCGTTGACTTCGCGCACGGGGCGTTGGCCACGTCCACAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCa                                                                                                                                          >  1:1278260/1‑150 (MQ=255)
           tttGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGGCGTCGGCCa                                                                                                                                          >  1:1514401/1‑150 (MQ=255)
                  tGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCCGGCCCGCGCCCGCAGcgaaa                                                                                                                                        <  2:878625/145‑5 (MQ=255)
                  tGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCCGGCCCGCGCCCGCAGcgaaa                                                                                                                                        >  1:878625/1‑141 (MQ=255)
                     ggCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCg                                                                                                                                <  1:1598401/150‑1 (MQ=255)
                      gCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCgg                                                                                                                               >  1:446643/1‑150 (MQ=255)
                          gTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAg                                                                                                                           >  1:852809/1‑150 (MQ=255)
                          gTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAg                                                                                                                           <  1:957333/150‑1 (MQ=255)
                             cAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTa                                                                                                                        >  1:906125/1‑150 (MQ=255)
                                  ccaggcccaggcTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCa                                                                                                                  <  2:1841826/150‑1 (MQ=255)
                                    aggcccaggcTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGc                                                                                                                <  1:997417/150‑1 (MQ=255)
                                    aggcccaggcTGGCGTTTTCTGGCTGGGAGAAGAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGc                                                                                                                >  1:527647/1‑150 (MQ=255)
                                              tGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCtt                                                                                                      >  1:1098824/1‑150 (MQ=255)
                                               ggCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTg                                                                                                     <  2:1892114/150‑1 (MQ=255)
                                                   ttttCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTggcgg                                                                                                 <  1:936305/150‑1 (MQ=255)
                                                         ggctggGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGTCCGGCAGCCATTTCCCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAgc                                                                                           <  1:1504952/150‑1 (MQ=255)
                                                         ggctggGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAgc                                                                                           <  1:177801/150‑1 (MQ=255)
                                                          gctggGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCTATTTACGATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAgct                                                                                          >  2:850454/1‑150 (MQ=255)
                                                          gctggGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGGTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGATGACTGCCTTGGCGGCGCAgct                                                                                          >  1:97978/1‑150 (MQ=255)
                                                             gggCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAgctgct                                                                                       <  2:771584/150‑1 (MQ=255)
                                                                ccAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCCGGCCCGCGcc                                                                                                                                                  <  2:1654254/89‑1 (MQ=255)
                                                                  agcagGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAgg                                                                                  >  1:1090969/1‑150 (MQ=255)
                                                                  agcagGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAgg                                                                                  >  1:1253788/1‑150 (MQ=255)
                                                                         tGCCGTGGCGGGTCTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGt                                                                           <  2:204879/150‑1 (MQ=255)
                                                                              gggCGGGTGTCGTACATCACTTCGCGCACGGGAGGCGAGGCAGGTACAAAGTAGGTCTCGCTGCGGGCGCGGGCCGGCAGCGATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGa                                                                      <  2:1095359/149‑1 (MQ=255)
                                                                                  gggTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAg                                                                                                                       <  1:115474/98‑1 (MQ=255)
                                                                                  gggTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAg                                                                                                                       >  2:115474/1‑98 (MQ=255)
                                                                                      gtCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGc                                                              <  1:1122210/150‑1 (MQ=255)
                                                                                       tCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGcc                                                             >  2:1446281/1‑150 (MQ=255)
                                                                                              tCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAg                                                                             <  1:1221867/127‑1 (MQ=255)
                                                                                              tCACTTCGCGCAAGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAg                                                                             >  2:1221867/1‑127 (MQ=255)
                                                                                                  ttCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCgct                                                  >  1:852234/1‑150 (MQ=255)
                                                                                                  ttCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGCCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGGCCTGATTGTCGGCCATGACCGCgct                                                  >  2:950062/1‑150 (MQ=255)
                                                                                                    cgcgCACGGGGCGTTGGCCACTTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTGAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCgctgc                                                <  2:619196/150‑1 (MQ=255)
                                                                                                         aCGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTc                                           <  2:569864/150‑1 (MQ=255)
                                                                                                            cggCCTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCGTCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCCAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAg                                        <  1:598322/149‑1 (MQ=255)
                                                                                                               cGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGcc                                     <  2:1228928/150‑1 (MQ=255)
                                                                                                                gTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGa                                                                      >  2:891517/1‑116 (MQ=255)
                                                                                                                gTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGa                                                                      <  1:891517/116‑1 (MQ=255)
                                                                                                                    gCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGAGAGTCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCtt                                <  1:310819/150‑1 (MQ=255)
                                                                                                                       aCGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGcc                             >  1:1689769/1‑150 (MQ=255)
                                                                                                                              aaGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCCTCGGCCAGGTAG‑CTCCGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAg                      <  2:1404432/150‑1 (MQ=255)
                                                                                                                                gTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTa                       <  1:991889/147‑1 (MQ=255)
                                                                                                                                gTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTa                       >  2:991889/1‑147 (MQ=255)
                                                                                                                                gTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGcc                    <  2:852234/150‑1 (MQ=255)
                                                                                                                                 tAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCg                   >  1:742512/1‑150 (MQ=255)
                                                                                                                                 tAGGTTTCGCGGCGGGCGCGGGCCGGCAGCCATTTACAATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCg                   <  2:308958/150‑1 (MQ=255)
                                                                                                                                    gTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCa                                                                                                                            >  2:1364796/1‑43 (MQ=255)
                                                                                                                                    gTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCa                                                                                                                            <  1:1364796/43‑1 (MQ=255)
                                                                                                                                    gTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCa                            <  2:335575/138‑1 (MQ=255)
                                                                                                                                    gTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCa                            >  1:335575/1‑138 (MQ=255)
                                                                                                                                    gTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGcc                >  1:1752307/1‑150 (MQ=255)
                                                                                                                                    gTTTCGCTCCGAGAACGTGACAGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGcc                >  2:78970/1‑150 (MQ=255)
                                                                                                                                     gttCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCCGCCAGATAG‑CTACCAAGACTGCCTTGGCGGCTCGGCTGCTCGAGGAACAGGTCCTGATTTTCGGCCAGGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCa               >  1:1469882/2‑150 (MQ=255)
                                                                                                                                       tggCTGCCGGCCCGCGCCCGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTTCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGc             >  1:100045/3‑150 (MQ=255)
                                                                                                                                         gCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAAGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCATCTTGCCATTGTAGCCGGCCAGcac           >  2:905577/1‑150 (MQ=255)
                                                                                                                                           tGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGATGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACaa         >  2:1499085/1‑150 (MQ=255)
                                                                                                                                              gggcgcgggcCGGCAGCCATTTACCATCGGCCAGGTAGACACCATTAATAGTATTCG‑GGGGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACAATTg      <  2:527667/150‑1 (MQ=255)
                                                                                                                                               ggcgcgggcCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACAATTgg     <  2:97978/150‑1 (MQ=255)
                                                                                                                                                  gcgggcCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACAATTGGCAg  <  2:742512/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTGCGATCACCTTTGAACTGGGGCAGGTTCAGCGCCAGGCCCAGGCTGGCGTTTTCTGGCTGGGCCAGCAGGGTGCCGTGGCGGGTGTCGTACATCACTTCGCGCACGGGGCGTTGGCCACGTACAAAGTAGGTTTCGCTGCGGGCGCGGGCCGGCAGCCATTTACCATCGGCCAGGTAG‑CTCAGCTGACTGCCTTGGCGGCGCAGCTGCTCGAGGAACAGGTCCTGATTGTCGGCCATGACCGCGCTGCTGCTCCAGGCCAGCTTGCCATTGTAGCCGGCCAGCACAATTGGCAG  >  NC_002947/1266774‑1267069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑