Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,293,261 T→G 5.9% A286A (GCT→GCG)  PP_1128 → OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,293,2610TG 5.9% 232.4 / 4.7 84A286A (GCT→GCG) PP_1128OmpA family protein
Reads supporting (aligned to +/- strand):  ref base T (33/46);  new base G (3/2);  total (36/48)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01

CGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGC  >  NC_002947/1293117‑1293410
                                                                                                                                                 |                                                                                                                                                     
cGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCaa                                                                                                                                                  <  1:445509/150‑1 (MQ=255)
cGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCaa                                                                                                                                                  <  1:1347460/150‑1 (MQ=255)
  aGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCCGGCCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGATGCCAAgg                                                                                                                                                <  1:398378/150‑1 (MQ=255)
   gggCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGc                                                                                                                                               <  2:1230446/150‑1 (MQ=255)
      ccGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACAAACAGGCCGCCCCCG‑ACTCGCCCCTGTCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGc                                                                                                                                            <  1:233065/150‑1 (MQ=255)
       cgcgCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCt                                                                                                                                           >  1:624144/1‑150 (MQ=255)
           cccTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGAAGACCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCtggtg                                                                                                                                       <  1:128074/150‑1 (MQ=255)
            ccTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCtggtgg                                                                                                                                      >  2:1926164/1‑150 (MQ=255)
              tGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCa                                                                                                                                    >  2:1588590/1‑150 (MQ=255)
                      cgcgTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGcc                                                                                                                            <  2:1457220/150‑1 (MQ=255)
                       gcgTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGcc                                                                                                                            >  1:480429/1‑149 (MQ=255)
                       gcgTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGcc                                                                                                                            <  2:480429/149‑1 (MQ=255)
                                   tGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTgcgg                                                                                                               <  2:624144/150‑1 (MQ=255)
                                       gACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCTC‑GGTTATGTTGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaa                                                                                                           <  1:1554621/150‑1 (MQ=255)
                                        aCCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaag                                                                                                          >  1:1428274/1‑150 (MQ=255)
                                         ccccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCtggtg                                                                                                                                       <  2:689370/120‑1 (MQ=255)
                                         ccccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCtggtg                                                                                                                                       >  1:689370/1‑120 (MQ=255)
                                         ccccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaaga                                                                                                         >  1:916768/1‑150 (MQ=255)
                                          cccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaagaa                                                                                                        >  1:797845/1‑150 (MQ=255)
                                           ccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGaaa                                                                                                       <  2:916768/150‑1 (MQ=255)
                                           ccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGaaa                                                                                                       >  1:714086/1‑150 (MQ=255)
                                            cGCAAAACACCATGGTCGCGCTGCTGGCCAAATCCCAGCAGGTCGTGCCAACCATAAAGCC‑GATACGCATGGTGGAGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAAc                                                                                                      <  1:697059/150‑1 (MQ=255)
                                              cAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCt                                                                                                    <  2:1255500/150‑1 (MQ=255)
                                              cAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCt                                                                                                    <  2:797845/150‑1 (MQ=255)
                                               aaaaCACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTg                                                                                                   <  2:551294/150‑1 (MQ=255)
                                                 aaCACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTa                                                                                                 >  2:866311/1‑150 (MQ=255)
                                                  acacCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAg                                                                                                <  2:955416/150‑1 (MQ=255)
                                                      cATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCaa                                                                                            >  2:356781/1‑150 (MQ=255)
                                                      cATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCaa                                                                                            >  1:532447/1‑150 (MQ=255)
                                                       aTGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCt                                                                                                                               >  1:622611/1‑114 (MQ=255)
                                                       aTGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCt                                                                                                                               <  2:622611/114‑1 (MQ=255)
                                                        tGGTCGCTCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑AGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGCTGGCAAACCTGCCGTCAAGGCTGCGGCGAACAAACCTGTAGCCAAgg                                                                                          <  2:977506/150‑1 (MQ=255)
                                                        tGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAgg                                                                                          <  1:461026/150‑1 (MQ=255)
                                                           tCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCa                                                                                       >  2:4033/1‑150 (MQ=255)
                                                                 tgctgGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGc                                                                                 >  1:1426743/1‑150 (MQ=255)
                                                                   ctgGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAg                                                                                                                      <  2:698866/111‑1 (MQ=255)
                                                                   ctgGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGATGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAg                                                                                                                      >  1:698866/1‑111 (MQ=255)
                                                                    tgGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTg                                                                              <  2:624653/150‑1 (MQ=255)
                                                                       ccAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCGGCCTTGGCAGCtgg                                                                                                                                         >  1:564542/1‑88 (MQ=255)
                                                                       ccAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCGGCCTTGGCAGCtgg                                                                                                                                         <  2:564542/88‑1 (MQ=255)
                                                                        cAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGaa                                                                          >  1:565821/1‑150 (MQ=255)
                                                                        atttggccAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCACGGCCGCGGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCaaaa                                                                                    <  2:1205670/134‑1 (MQ=255)
                                                                        atttggccAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCaaaa                                                                                    >  1:1205670/7‑140 (MQ=255)
                                                                          aaTACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGa                                                                        <  1:866311/150‑1 (MQ=255)
                                                                           aTACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGAc                                                                       >  1:1601859/1‑150 (MQ=255)
                                                                              ccAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGc                                                                    >  1:1844708/1‑150 (MQ=255)
                                                                              ccAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGc                                                                    >  2:1875355/1‑150 (MQ=255)
                                                                               cagcagGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCa                                                                   <  1:1141405/150‑1 (MQ=255)
                                                                                 gcagGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGCTGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGc                                                                 >  2:1161888/1‑150 (MQ=255)
                                                                                    ggCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCaa                                                              <  2:1884445/150‑1 (MQ=255)
                                                                                    ggCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCaa                                                              <  2:83104/150‑1 (MQ=255)
                                                                                    ggCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTAAGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCaa                                                              <  1:800999/150‑1 (MQ=255)
                                                                                       cgccCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGGCGTCAAGGCTGCGGCGAAGAAAc                                                                                                      <  2:940873/107‑1 (MQ=255)
                                                                                       cgccCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAAc                                                                                                      >  1:940873/1‑107 (MQ=255)
                                                                                           ccGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGaaaaaa                                                       <  2:1428274/150‑1 (MQ=255)
                                                                                            cGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAg                                                                                                <  2:47778/108‑1 (MQ=255)
                                                                                            cGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAg                                                                                                >  1:47778/1‑108 (MQ=255)
                                                                                              gCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCt                                                    <  1:971655/150‑1 (MQ=255)
                                                                                                    gCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCg                                              >  2:166609/1‑150 (MQ=255)
                                                                                                       cc‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGt                                                                                                  >  2:827087/1‑95 (MQ=255)
                                                                                                       cc‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGt                                                                                                  <  1:827087/95‑1 (MQ=255)
                                                                                                       cc‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCCGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGcc                                           >  2:1421843/1‑150 (MQ=255)
                                                                                                             cTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAGAGCTGCGCCGGCCAAGcc                                      >  2:1003850/1‑150 (MQ=255)
                                                                                                               cGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTg                                    <  2:155517/150‑1 (MQ=255)
                                                                                                                      ggcggcGCAGGATGCCAAGGCACCAGCTGCCAAGCCCGCGGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGTCCAAACCGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCaa                             <  1:810091/150‑1 (MQ=255)
                                                                                                                      ggCGTTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTCGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCaa                             <  1:483137/150‑1 (MQ=255)
                                                                                                                          gTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGaaa                         <  1:1173513/150‑1 (MQ=255)
                                                                                                                             cAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCt                      <  1:1503735/150‑1 (MQ=255)
                                                                                                                               ggATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGc                    <  1:795920/150‑1 (MQ=255)
                                                                                                                                gATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGTAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGcc                   >  2:1900415/1‑150 (MQ=255)
                                                                                                                                     cAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGa              >  2:708217/1‑150 (MQ=255)
                                                                                                                                     cAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGNTGa              <  2:1194131/150‑1 (MQ=255)
                                                                                                                                      aaGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGa              <  1:656148/149‑1 (MQ=255)
                                                                                                                                      aaGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGa              >  2:656148/1‑149 (MQ=255)
                                                                                                                                          cACCAGCGGCCTTGGCAGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGa              >  2:1582753/1‑145 (MQ=255)
                                                                                                                                          cACCAGCGGCCTTGGCAGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGa              <  1:1582753/145‑1 (MQ=255)
                                                                                                                                           aCCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGaa        <  2:317834/150‑1 (MQ=255)
                                                                                                                                           aCCAGCGGCCTTGGCAGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGAaaaaaa        >  1:1012571/1‑150 (MQ=255)
                                                                                                                                            ccAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGaaa       <  1:166609/150‑1 (MQ=255)
                                                                                                                                            ccAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGaaa       <  1:1421843/150‑1 (MQ=255)
                                                                                                                                             cAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTgc                                                                                                                 <  1:513293/43‑1 (MQ=255)
                                                                                                                                             cAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTgc                                                                                                                 >  2:513293/1‑43 (MQ=255)
                                                                                                                                             cAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAg      >  1:1896037/1‑150 (MQ=255)
                                                                                                                                                cTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAg   <  2:1346828/150‑1 (MQ=255)
                                                                                                                                                 tGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAACAGCTGTGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGc  <  2:1601859/150‑1 (MQ=255)
                                                                                                                                                 tGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGc  >  2:1304306/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCC‑GGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGC  >  NC_002947/1293117‑1293410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑