Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,294,120 C→T 9.8% intergenic (+15/+25) pdxH → / ← PP_1130 pyridoxine/pyridoxamine 5'‑phosphate oxidase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,294,1200CT 9.8% 132.3 / 4.6 51intergenic (+15/+25)pdxH/PP_1130pyridoxine/pyridoxamine 5'‑phosphate oxidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (22/24);  new base T (2/3);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

CTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCC  >  NC_002947/1293972‑1294265
                                                                                                                                                    |                                                                                                                                                 
cTGAGCATT‑GGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTAGACGGGCATTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGAGCCGCCaa                                                                                                                                                 <  1:1774339/150‑1 (MQ=255)
 tGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCaa                                                                                                                                                 <  2:128446/150‑1 (MQ=255)
   cgccttGGGGGGGATACCGCTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGc                                                                                                                                               <  1:270199/146‑1 (MQ=255)
   aGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCTGCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGc                                                                                                                                               <  2:150737/150‑1 (MQ=255)
          ggggggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCGTGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCTTGGCGGCCCCa                                                                                                                                        <  2:1740571/150‑1 (MQ=255)
          ggggggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCa                                                                                                                                        >  1:155056/1‑150 (MQ=255)
          ggggggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCa                                                                                                                                        >  1:1729758/1‑150 (MQ=255)
           gggggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAg                                                                                                                                       >  1:1028485/1‑150 (MQ=255)
                  aCCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGTGGCCCCAGCCATTGa                                                                                                                                <  2:778617/150‑1 (MQ=255)
                   ccGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGAt                                                                                                                               <  2:1236499/150‑1 (MQ=255)
                    cGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATc                                                                                                                              >  1:1818637/1‑150 (MQ=255)
                       ttgttgCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTCGGGCCGCCAAGCGGCCCCAGCCATTGATCAAg                                                                                                                           <  2:1070517/150‑1 (MQ=255)
                          atgCCCGAGCGCATCGAGGTCGGGCATGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTa                                                                                                                        >  2:25397/2‑150 (MQ=255)
                                      agcGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAAcc                                                                                                            <  2:185758/148‑1 (MQ=255)
                                            ttCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCTTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTgccgc                                                                                                      <  2:1055067/150‑1 (MQ=255)
                                               tGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGGCGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTc                                                                                                   >  2:88537/1‑150 (MQ=255)
                                                 gCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGACGCCTccc                                                                                                 >  2:521664/1‑148 (MQ=255)
                                                    ggggCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCt                                                                                              <  2:155056/150‑1 (MQ=255)
                                                         gggcAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCTCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCacc                                                                                         <  2:1818937/150‑3 (MQ=255)
                                                           gcAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAacca                                                                                       >  1:1020003/1‑150 (MQ=255)
                                                               gtcgtcTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCAcccc                                                                                   >  1:510488/1‑150 (MQ=255)
                                                                    cTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAg                                                                              <  2:1028485/150‑1 (MQ=255)
                                                                     tGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGt                                                                             >  1:1251629/1‑150 (MQ=255)
                                                                         tGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAGGTGCCGCACTCGATTTAGTATGCTGCCCTGGGTCACATGGACCCTCCCGCACCAACCACCCCGCCAGTTGCt                                                                         >  1:975175/1‑150 (MQ=255)
                                                                           aTCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTgccgc                                                                                                      >  1:1882922/1‑119 (MQ=255)
                                                                           aTCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTgccgc                                                                                                      <  2:1882922/119‑1 (MQ=255)
                                                                              gTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGc                                                                    >  2:818938/1‑150 (MQ=255)
                                                                                 tGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGcccc                                                                 <  1:1491381/150‑1 (MQ=255)
                                                                                     cTATCGGCTGGTGGACGAGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCACAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCCCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCt                                                             <  2:91874/150‑1 (MQ=255)
                                                                                      tATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCtt                                                            >  2:200712/1‑150 (MQ=255)
                                                                                      tATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCtt                                                            >  2:1913245/1‑150 (MQ=255)
                                                                                              ggtggACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGcc                                                    >  1:1818912/1‑150 (MQ=255)
                                                                                              ggtggACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGcc                                                    <  2:1818912/150‑1 (MQ=255)
                                                                                                tggACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCcgc                                                  <  1:818938/150‑1 (MQ=255)
                                                                                                  gACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCgcgc                                                <  1:1134866/150‑1 (MQ=255)
                                                                                                   aCGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGcc                                               >  2:852221/1‑150 (MQ=255)
                                                                                                   aCGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGcc                                               >  2:1828881/1‑150 (MQ=255)
                                                                                                            ggCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAAc                                      <  2:1251629/150‑1 (MQ=255)
                                                                                                             gCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAAcc                                     >  1:1230198/1‑150 (MQ=255)
                                                                                                               tGCGAGAGCTGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCtt                                   <  1:88537/150‑1 (MQ=255)
                                                                                                                   agagCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCa                               <  1:1898793/150‑1 (MQ=255)
                                                                                                                                 ccTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGt                 >  2:1599370/1‑150 (MQ=255)
                                                                                                                                      gCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTc            <  2:1818637/150‑1 (MQ=255)
                                                                                                                                       cAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGTGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCa           <  2:975175/150‑1 (MQ=255)
                                                                                                                                         gcTGGGGCCGCTTGGCGGCCCCAGTCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCAcccc                                                                                   <  2:1486864/75‑1 (MQ=37)
                                                                                                                                         gcTGGGGCCGCTTGGCGGCCCCAGTCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCAcccc                                                                                   >  1:1486864/2‑76 (MQ=37)
                                                                                                                                           tGGGGCCGCTTGGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCa           <  2:124348/146‑1 (MQ=255)
                                                                                                                                           tGGGGCCGCTTGGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTGGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCa           >  1:124348/1‑146 (MQ=255)
                                                                                                                                           tGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAg                                                                              <  2:1626113/79‑1 (MQ=255)
                                                                                                                                           tGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAg                                                                              >  1:1626113/1‑79 (MQ=255)
                                                                                                                                           tGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGAt       >  1:1048371/1‑150 (MQ=255)
                                                                                                                                               gccgccAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGTATCGGc   >  1:338249/1‑150 (MQ=255)
                                                                                                                                                ccgccAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGcc  >  1:1490799/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAGGATCGGCC  >  NC_002947/1293972‑1294265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑