Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,449,147	G→A	7.3%	*287* (TGA→TAA) ‡	PP_1266 →	fusaric acid resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,449,147	0	G	A	7.3%	149.2 / 2.0	55	*287* (TGA→TAA) ‡	PP_1266	fusaric acid resistance protein
Reads supporting (aligned to +/- strand):  ref base G (25/25);  new base A (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

GATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCTT  >  NC_002947/1449004‑1449289                                                                                                                                                |                                                                                                                                               gATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTc                                                                                                                                          <  2:1908917/150‑1 (MQ=255) gATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTCAACCTTc                                                                                                                                          <  2:147450/150‑1 (MQ=255)      ccGACGTGAACCCGACCTTCAACTGTGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGAc                                                                                                                                     >  2:1577979/1‑150 (MQ=255)        gACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCa                                                                                                                                   >  1:203990/1‑150 (MQ=255)               aCCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGc                                                                                                                            >  1:336765/1‑150 (MQ=255)               aCCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGc                                                                                                                            >  1:1613460/1‑150 (MQ=255)               aCCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGc                                                                                                                            >  1:1608238/1‑150 (MQ=255)                cccGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATGCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGcc                                                                                                                           >  2:741413/1‑150 (MQ=255)                cccGACCTTCAACGGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCAGTCACGTCGATGAAGTGCCGGGAGGGGTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGcc                                                                                                                           >  2:1505991/1‑150 (MQ=255)                   gACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTcc                                                                                                                        >  1:572472/1‑150 (MQ=255)                    aCCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTccc                                                                                                                       <  2:1828257/150‑1 (MQ=255)                      cTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGc                                                                                                                     >  1:1062020/1‑150 (MQ=255)                                     gCTGGCACAGCGGGTGCTGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGc                                                                                                      <  2:1743804/150‑1 (MQ=255)                                     gCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAACTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTGAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGc                                                                                                      >  1:841578/1‑150 (MQ=255)                                          cacaGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCctgc                                                                                                 <  1:741413/150‑1 (MQ=255)                                           acaNCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCctgct                                                                                                >  2:1199528/1‑150 (MQ=255)                                              gCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGat                                                                                             <  1:77265/150‑1 (MQ=255)                                                    gCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGatgcatgc                                                                                       <  1:726389/150‑1 (MQ=255)                                                        gTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTAt                                                                                   <  2:203990/150‑1 (MQ=255)                                                          gcgcATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTa                                                                                 <  1:900104/150‑1 (MQ=255)                                                            gcATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGc                                                                               >  1:641052/1‑150 (MQ=255)                                                            gcATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGc                                                                               <  2:624959/150‑1 (MQ=255)                                                             cATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGcc                                                                              >  2:1529325/1‑150 (MQ=255)                                                              aTTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCa                                                                             <  1:1199528/150‑1 (MQ=255)                                                              aTTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCa                                                                             <  2:1494643/150‑1 (MQ=255)                                                                 cacaTCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTc                                                                          <  1:197673/150‑1 (MQ=255)                                                                 cacaTCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTc                                                                          <  2:918099/150‑1 (MQ=255)                                                                  acaTCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTCAACCTTcgc                                                                                                                                        <  2:1605823/87‑1 (MQ=255)                                                                  acaTCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTCAACCTTcgc                                                                                                                                        >  1:1605823/1‑87 (MQ=255)                                                                     tCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCt                                                                      >  1:1696173/1‑150 (MQ=255)                                                                        aTGAAGTGCCGGAAGGGTTTTTTTTTTTTTTTGGGACGACGTGTACGGTGGTAGTCAAGCCTAGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCaa                                                                   >  2:1105935/1‑150 (MQ=255)                                                                          gAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCctgc                                                                                                 <  2:1771763/118‑1 (MQ=255)                                                                          gAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTAGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCctgc                                                                                                 >  1:1771763/1‑118 (MQ=255)                                                                             gTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGc                                                              >  1:561777/1‑150 (MQ=255)                                                                                   gAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGc                                                        >  1:452304/1‑150 (MQ=255)                                                                                         tttttGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTg                                                  >  1:1541507/1‑150 (MQ=255)                                                                                         tttttGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTg                                                  <  2:561777/150‑1 (MQ=255)                                                                                           tttGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCATTCTTTTGCATCCTCTTgca                                                <  2:1608238/150‑1 (MQ=255)                                                                                           tttGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTgca                                                >  1:1111012/1‑150 (MQ=255)                                                                                           tttGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTgca                                                <  2:1613460/150‑1 (MQ=255)                                                                                                    ggcggGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGt                                       <  1:1577979/150‑1 (MQ=255)                                                                                                       ggggACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTccc                                    >  1:1689576/1‑150 (MQ=255)                                                                                                              acgTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTc                             <  2:1124639/150‑1 (MQ=255)                                                                                                                      ggtggtAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGt                     <  1:1580894/150‑1 (MQ=255)                                                                                                                           tAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCg                <  2:1702963/150‑1 (MQ=255)                                                                                                                             gTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCAGCATCATCAGAAGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCGCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCt              >  1:707238/1‑150 (MQ=255)                                                                                                                                 aGCCTGGCGAAGGTTGACCTTCGCGACTATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGc          <  2:641363/150‑1 (MQ=255)                                                                                                                                   ccTGGCGAAGGTTTTTTTTTGACATCATGCCGCCCGCCTGCATCATCTGTGGGGGCCCTGCTGATGCATGCGTATTAGCCGGTGGGCCCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCaa        <  2:713077/150‑1 (MQ=255)                                                                                                                                   ccTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTAt            <  1:464/146‑1 (MQ=255)                                                                                                                                   ccTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTAt            >  2:464/1‑146 (MQ=255)                                                                                                                                     tcgCGAAGGTCAACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACa      >  2:1216639/3‑150 (MQ=255)                                                                                                                                        cGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCt   >  2:1555224/1‑150 (MQ=255)                                                                                                                                         gAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAAc                                           <  1:194057/109‑1 (MQ=255)                                                                                                                                         gAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAAc                                           >  2:194057/1‑109 (MQ=255)                                                                                                                                         gAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCtt  <  2:641052/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                               GATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCTT  >  NC_002947/1449004‑1449289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑