Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,544,531 C→A 15.8% intergenic (+48/+66) mdfA → / ← ampG multidrug MFS transporter/muropeptide permease AmpG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,544,5310CA15.8% 120.6 / 11.5 49intergenic (+48/+66)mdfA/ampGmultidrug MFS transporter/muropeptide permease AmpG
Reads supporting (aligned to +/- strand):  ref base C (15/26);  new base A (4/4);  total (19/30)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

GCATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCA  >  NC_002947/1544384‑1544676
                                                                                                                                                   |                                                                                                                                                 
gcATTTTGCCCTGGCGGGGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAg                                                                                                                                                 <  1:503087/150‑1 (MQ=255)
gcATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAg                                                                                                                                                 <  2:1805043/150‑1 (MQ=255)
 cATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAgg                                                                                                                                                <  1:657025/150‑1 (MQ=255)
        cccTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccgcgc                                                                                                                                         >  2:87196/1‑150 (MQ=255)
              cggcggGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCttt                                                                                                                                   <  1:1618468/150‑1 (MQ=255)
                 cggGGGTGGGGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTa                                                                                                                                <  2:188262/150‑1 (MQ=255)
                  gggggTGGCGGGGGCATTGGTGCTGTGGCCGAGGGGCTTAGTGGTGGGGGCGCGGCCTGGGGAAGGGGCTGTAGCTCGCTAATCGGCACTGCCGGGCCCGGCCTCTACGCGGGTAAACCCGCTCCGACGAAGCGGCGCGGCCCTGTGTAg                                                                                                                               >  2:821164/1‑150 (MQ=16)
                       gggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCg                                                                                                                          <  2:1353374/149‑1 (MQ=18)
                        ggCGGGGGCGTTGGCGCTGTGGCTGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCgg                                                                                                                         <  1:821164/150‑1 (MQ=25)
                        ggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGANCgg                                                                                                                         <  2:1190526/150‑1 (MQ=255)
                        ggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCgg                                                                                                                         <  1:746518/150‑1 (MQ=255)
                        ggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCgg                                                                                                                         <  2:1593455/150‑1 (MQ=25)
                        ggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGATGTTTTGCGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCgg                                                                                                                         <  2:1483423/150‑1 (MQ=39)
                         ggggggggGTTGGCCTGGAGGCCGCTGTGGTTTTCTGGGGGGGCGAGGCTTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAACCCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCggg                                                                                                                        <  2:1258385/148‑1 (MQ=255)
                         gCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGTCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCggg                                                                                                                        <  1:809389/150‑1 (MQ=255)
                         gCGGGGGCGTTGGCGCTGTGGCCGATGTATTGTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCggg                                                                                                                        <  1:941313/150‑1 (MQ=255)
                                ggTTGGCGCGGGGGCCGCTGGGTTTTGTGGGGGGGGGGGGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTAccc                                                                                                                 <  2:1210825/149‑1 (MQ=18)
                                   tGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCcgcg                                                                                                              <  2:1138269/150‑1 (MQ=255)
                                          ggggcggcGGTGGTTTGGGGGGGGTGGGCGGCCGGGGGAGGGGGCTGTAGCTGGATAATCGGTATTGCCGGGGCCGGCCGCTTCGCGGGTAACCCCGATCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGc                                                                                                       <  2:648344/148‑1 (MQ=25)
                                             gCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGTCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAg                                                                                                    >  1:191462/1‑150 (MQ=255)
                                                 gggggtgttGTGGAGGG‑GGGTGTCCTGGGTGGGGGGCGGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAACCCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAg                                                                                               <  2:1849166/143‑1 (MQ=21)
                                                    tgGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAg                                                                                                                               >  2:502144/1‑116 (MQ=255)
                                                    tgGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAg                                                                                                                               <  1:502144/116‑1 (MQ=255)
                                                        ttGTGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATa                                                                                         <  1:87196/150‑1 (MQ=255)
                                                            ggtgggggGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACaaa                                                                                     <  1:84883/150‑1 (MQ=255)
                                                             gtgggtggGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAg                                                                                    <  2:484560/150‑1 (MQ=255)
                                                             gggggtgggCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTACGCGCAGTTGCTCGCTGCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAg                                                                                    <  2:1097166/148‑1 (MQ=37)
                                                                  tgggCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAggg                                                                                                                                               <  2:1706496/86‑1 (MQ=255)
                                                                  tgggCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAggg                                                                                                                                               >  1:1706496/1‑86 (MQ=255)
                                                                           tGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCggg                                                                                                                        >  2:996528/1‑100 (MQ=255)
                                                                           tGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCggg                                                                                                                        <  1:996528/100‑1 (MQ=255)
                                                                                                            gCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGTCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTccc                                     <  2:191462/150‑1 (MQ=255)
                                                                                                                    tggccTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGa                                                                                                                             >  1:487836/2‑54 (MQ=255)
                                                                                                                    tggccTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGa                                                                                                                             <  2:487836/53‑1 (MQ=255)
                                                                                                                           ttCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAATACAAAGCTTCAGGGCCGCTCCCCCACCGAGACGTGGGCCGCCAGCCTCGTCCCCCGCCCGGCTTCCTg                      >  2:1647611/1‑150 (MQ=14)
                                                                                                                               cgGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGAtcc                  <  2:1663744/150‑3 (MQ=255)
                                                                                                                                gggTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGcc                 >  2:97815/1‑150 (MQ=255)
                                                                                                                                   tAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTCATCGGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACt              >  1:1279556/1‑150 (MQ=255)
                                                                                                                                    aaaCCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTg             >  1:84758/1‑150 (MQ=25)
                                                                                                                                       cccGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGACACTGCAg          >  2:1044576/1‑150 (MQ=255)
                                                                                                                                        gcacTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg         >  1:833652/4‑150 (MQ=255)
                                                                                                                                        ccGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg         >  2:64101/1‑150 (MQ=255)
                                                                                                                                        ccGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCAACCCCAGTCATGGCCCTCCCGGCTTCCTGATGCCACTGCAgg         >  1:887273/1‑150 (MQ=38)
                                                                                                                                        ccGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg         >  1:1275804/1‑150 (MQ=25)
                                                                                                                                             ccTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgat    <  1:1044576/150‑1 (MQ=255)
                                                                                                                                              cTACACAGGGCCGCGCCGCTTTGTTGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCcccgcc                                >  1:1533298/1‑121 (MQ=255)
                                                                                                                                              cTACACAGGGCCGCGCCGCTTTGTTGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCcccgcc                                <  2:1533298/121‑1 (MQ=255)
                                                                                                                                              cTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatc   >  2:1619857/1‑150 (MQ=255)
                                                                                                                                               tACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca  >  1:668395/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCA  >  NC_002947/1544384‑1544676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑