Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,639,638 C→T 7.6% intergenic (‑186/‑125) czcR‑III ← / → PP_1439 response regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,639,6380CT 7.6% 144.0 / 2.3 54intergenic (‑186/‑125)czcR‑III/PP_1439response regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (26/23);  new base T (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

TGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCAAATACCTGCTGT  >  NC_002947/1639492‑1639787
                                                                                                                                                  |                                                                                                                                                     
tGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCcgc                                                                                                                                                    >  2:1107175/1‑150 (MQ=255)
     tGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAaga                                                                                                                                               <  2:1433066/150‑1 (MQ=25)
     tGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAaga                                                                                                                                               <  1:775321/150‑1 (MQ=25)
       aCATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGGAGGAACGGGGTTAACACAGAAGAgg                                                                                                                                             >  1:1514940/1‑150 (MQ=255)
             gggTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGaa                                                                                                                                       <  2:1139809/150‑1 (MQ=255)
              ggTTAAGCGCCCTAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAAc                                                                                                                                      <  2:187762/150‑1 (MQ=255)
                ccAACCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAg                                                                                                                                    <  2:1433955/149‑1 (MQ=255)
                  acgcgcCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTTTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTc                                                                                                                                  <  1:178093/148‑1 (MQ=255)
                    gcgcCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCca                                                                                                                                <  2:1864618/150‑1 (MQ=255)
                    gcgcCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGGTTACCCGCGAAGAGGCCCGACCAGTCca                                                                                                                                >  2:925489/1‑150 (MQ=255)
                         ccAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACaa                                                                                                                           <  2:178289/150‑1 (MQ=255)
                               ttACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCcac                                                                                                                     >  2:173348/1‑150 (MQ=255)
                                      aTGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACACGCGCCTGTAGGAACGGGGTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAg                                                                                                              >  1:378870/1‑150 (MQ=255)
                                          tgacaGGCCGGCGGGGCAGTGAGCGGATGGGGAATGCTGCTGTGGTCGGGCTGTCTGTTCGGGCCTCTGCGCGAGAAACCCCGGGCCTGTAGGAGCGGGTGTACGCGCGAAGAGGCCCGACCAGTCCACACAAGGCCACATTGCAGGACt                                                                                                          <  2:679626/146‑1 (MQ=255)
                                                   tGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATcccc                                                                                                 >  1:1700783/1‑150 (MQ=255)
                                                     cGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGc                                                                                               <  1:1642230/150‑1 (MQ=255)
                                                          cAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCAc                                                                                          <  1:300660/150‑1 (MQ=255)
                                                           aGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCAcc                                                                                         <  1:173348/150‑1 (MQ=255)
                                                             gataCGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTTGCGAGGTAACCCGCGCCTGTAGGAACGGGTTTACCCGGGAAGATGCCCGGACAGTCCACACAAGGCCACCTTGCAGGACTGTACACCCCGCCTCACCtt                                                                                       >  2:224261/4‑150 (MQ=255)
                                                               aaCGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGc                                                                                     <  2:1460246/150‑1 (MQ=255)
                                                                    aTTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTCTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTa                                                                                <  2:1387807/150‑1 (MQ=255)
                                                                       tGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGACGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTagcc                                                                             <  1:925489/150‑1 (MQ=255)
                                                                            ttCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGccc                                                                        >  1:1488911/1‑150 (MQ=255)
                                                                                       tCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATg                                                             >  1:409097/1‑150 (MQ=255)
                                                                                         tGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTa                                                                  <  2:1192900/143‑1 (MQ=255)
                                                                                         tGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTa                                                                  >  1:1192900/1‑143 (MQ=255)
                                                                                          ggCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGcc                                                          <  2:1631283/150‑1 (MQ=255)
                                                                                              gtctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACAGTGCAGGACTGTAATCCCCGCCTCACCTCGCCGTTAGCCAGCCCTCGCTAGGATGGCCgtgt                                                      >  2:1566110/1‑150 (MQ=255)
                                                                                              gtctgtTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGACCACAAACGGCTGCTCTAAACCAGTGTGAGCGCCTCCGCAGCTTGCAGTTCGCCAGCCCTCCCTAGGATGGCCgtgt                                                      >  1:223705/1‑150 (MQ=16)
                                                                                                       ggCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTACTCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGt                                             <  2:65923/150‑1 (MQ=255)
                                                                                                        gCCTCTTCGCGGGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAAGAGGCCCGAACAGACCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGtt                                            >  1:1140090/1‑150 (MQ=21)
                                                                                                        gCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCGGTGTCTGATCgaat                                            >  2:1151822/1‑147 (MQ=255)
                                                                                                         ccTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGtta                                           <  1:771498/150‑1 (MQ=255)
                                                                                                         ccTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGtta                                           >  1:1325765/1‑150 (MQ=255)
                                                                                                           tctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCg                                               >  2:1718936/1‑144 (MQ=255)
                                                                                                           tctTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCg                                               <  1:1718936/144‑1 (MQ=255)
                                                                                                             ttCGCGGGTAAACCCGTTCCTACAGGCGCGTGTTTACTCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCGGCCCTCGCTAGGATGGCCGTGTCTGATAGGTTATGAc                                       >  2:40709/1‑150 (MQ=25)
                                                                                                                gcgAGTAAACCCGCGCCTGTAGGAACGGGTTGACCCGCGAAGAGGCCCGAACAGTCCACACAAGTCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAg                                    >  1:447455/1‑150 (MQ=255)
                                                                                                                        aCCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTa                                                                                >  1:270258/1‑98 (MQ=255)
                                                                                                                        aCCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTa                                                                                <  2:270258/98‑1 (MQ=255)
                                                                                                                          ccGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGa                          >  2:1064747/1‑150 (MQ=255)
                                                                                                                          ccGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGa                          >  2:481240/1‑150 (MQ=255)
                                                                                                                           cgcgCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGAt                         <  2:1700783/150‑1 (MQ=255)
                                                                                                                            gcgcCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATg                        >  1:1056569/1‑150 (MQ=255)
                                                                                                                              gcCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGc                                                                                               <  1:1831127/77‑1 (MQ=255)
                                                                                                                              gcCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGc                                                                                               >  2:1831127/1‑77 (MQ=255)
                                                                                                                               ccTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAAc                     <  2:1488911/150‑1 (MQ=255)
                                                                                                                                cTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAgg                                                                                                                         <  1:782784/49‑1 (MQ=255)
                                                                                                                                cTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAgg                                                                                                                         >  2:782784/1‑49 (MQ=255)
                                                                                                                                   tAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGa                 >  2:1533711/1‑150 (MQ=255)
                                                                                                                                      gAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCa              >  2:1809883/1‑150 (MQ=255)
                                                                                                                                      gAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCAAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCa              >  2:719064/1‑150 (MQ=255)
                                                                                                                                         cGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCAAAt           <  2:1880923/150‑1 (MQ=255)
                                                                                                                                            gTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCAAATAcc        >  1:879014/1‑150 (MQ=255)
                                                                                                                                                  ccGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCAAATACCTGCTGt  >  2:796892/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TGTAGTGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCAAATACCTGCTGT  >  NC_002947/1639492‑1639787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑