Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,899,850 A→T 5.7% K120M (AAG→ATG) ‡ PP_1703 → assimilatory nitrate reductase/sulfite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,899,8500AT 5.7% 268.5 / 5.6 88K120M (AAG→ATG) ‡PP_1703assimilatory nitrate reductase/sulfite reductase
Reads supporting (aligned to +/- strand):  ref base A (41/42);  new base T (3/2);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

AGCCAGCCGCCAGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACGTATTTCTGG  >  NC_002947/1899702‑1899999
                                                                                                                                                    |                                                                                                                                                      
agccagccGCCAGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAg                                                                                                                                                       >  2:1701165/1‑150 (MQ=255)
   cagccGCCAGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTg                                                                                                                                                    <  1:675470/150‑1 (MQ=255)
   cagccGCCAGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTAGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTg                                                                                                                                                    <  2:1699987/150‑1 (MQ=255)
        gccAGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCaa                                                                                                                                               >  1:195875/1‑150 (MQ=255)
           aGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAggg                                                                                                                                            <  2:559110/150‑1 (MQ=255)
                  aCCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATc                                                                                                                                     <  1:315389/150‑1 (MQ=255)
                    cAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAgg                                                                                                                                             >  1:929035/1‑140 (MQ=255)
                    cAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAgg                                                                                                                                             <  2:929035/140‑1 (MQ=255)
                    cAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCg                                                                                                                                   >  2:11655/1‑150 (MQ=255)
                         tGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTAcgc                                                                                                                              <  2:526266/150‑1 (MQ=255)
                            aCCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGcc                                                                                                                           >  1:672885/1‑150 (MQ=255)
                              cAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACTTGTTGGCCag                                                                                                                                               <  1:1620811/128‑2 (MQ=255)
                              cAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACTTGTTGGCCag                                                                                                                                               >  2:1620811/1‑127 (MQ=255)
                              cAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGTCCAAGGGCTTTATCCGTACGCGCcac                                                                                                                         >  2:601638/1‑150 (MQ=255)
                                 gCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATc                                                                                                                      <  2:7065/150‑1 (MQ=255)
                                    aTCGCTGCCACCGCAGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAg                                                                                                                   <  2:634083/150‑1 (MQ=255)
                                    aTCGCAGCCACCGCCGAGCGTCGGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGGTGCACGTGTTCGGGCAAAGGTCGCTGGCTGCCCAGTACCTGGGCAACCAGTTGGCCAAGGGCTTGATCCGTACGCGGCACATCGAg                                                                                                                   >  2:765717/1‑150 (MQ=255)
                                      cGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTc                                                                                                                 <  1:1701165/150‑1 (MQ=255)
                                       gCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTcc                                                                                                                <  1:52332/150‑1 (MQ=255)
                                         tGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCaa                                                                                                              >  2:823432/1‑150 (MQ=255)
                                         tGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCaa                                                                                                              <  1:1531957/150‑1 (MQ=255)
                                            cACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACTTGTTGGCCag                                                                                                                                               <  1:1544573/114‑2 (MQ=255)
                                            cACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACTTGTTGGCCag                                                                                                                                               >  2:1544573/1‑113 (MQ=255)
                                            cACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTc                                                                                                           >  1:1293462/1‑150 (MQ=255)
                                                 ccgAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGcc                                                                                                      >  1:1260547/1‑150 (MQ=255)
                                                   gAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCg                                                                                                                                   >  2:1563494/1‑119 (MQ=255)
                                                   gAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCg                                                                                                                                   <  1:1563494/119‑1 (MQ=255)
                                                        tCTGCGCGGCATCATCGACCAGCCCGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCAt                                                                                               <  2:1315142/150‑1 (MQ=255)
                                                        tCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCAt                                                                                               <  1:1788257/150‑1 (MQ=255)
                                                         cTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATg                                                                                              <  1:1897109/150‑1 (MQ=255)
                                                         cTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATg                                                                                              <  1:699359/150‑1 (MQ=255)
                                                         cTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATg                                                                                              <  2:1027279/150‑1 (MQ=255)
                                                          tGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATgg                                                                                             <  1:845455/150‑1 (MQ=255)
                                                           gcgcGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGgc                                                                                            >  1:1615043/1‑150 (MQ=255)
                                                           gcgaGGCATCATAGATCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGgc                                                                                            <  2:1051784/150‑1 (MQ=255)
                                                             gcgGCATCATCGACCAGCACGGACCGGATGACGCGGCGCTGTCAGTGTCTGTTCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCAGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGgcca                                                                                          <  2:1260547/150‑1 (MQ=255)
                                                              cgGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTAcgc                                                                                                                              <  1:569902/113‑1 (MQ=255)
                                                              cgGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTAcgc                                                                                                                              >  2:569902/1‑113 (MQ=255)
                                                                  atcatcGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCCACTCGCGCCTGTGCATGgccagcgc‑c                                                                                    >  2:799621/1‑150 (MQ=255)
                                                                   tcatcGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGgccagcgc‑ca                                                                                   <  2:1353706/150‑1 (MQ=255)
                                                                    catcGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGgccagcgc‑cag                                                                                  >  2:587140/1‑150 (MQ=255)
                                                                     atcGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGgccagcgc‑cagc                                                                                 >  1:1386989/1‑150 (MQ=255)
                                                                      tcGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGTCAGCGC‑cagca                                                                                >  2:638062/1‑150 (MQ=255)
                                                                      tcGACCAGCACGGCCCGGATGCCGTAGAGTTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑cagca                                                                                >  1:1297362/1‑150 (MQ=255)
                                                                        gACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGt                                                                              >  2:1925118/1‑150 (MQ=255)
                                                                         aCCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTg                                                                             >  2:1457097/1‑150 (MQ=255)
                                                                         aCCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTg                                                                             <  1:803088/150‑1 (MQ=255)
                                                                         aCCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTg                                                                             >  2:34709/1‑150 (MQ=255)
                                                                           cAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGc                                                                           <  1:823432/150‑1 (MQ=255)
                                                                            aGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCgcgc                                                                                                       <  1:835649/122‑1 (MQ=255)
                                                                            aGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCgcgc                                                                                                       >  2:835649/1‑122 (MQ=255)
                                                                             gCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTa                                                                         >  2:147037/1‑150 (MQ=255)
                                                                                 ggCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAg                                                                     <  2:318074/150‑1 (MQ=255)
                                                                                   cccGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCt                                                                   <  1:877651/150‑1 (MQ=255)
                                                                                     cGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGt                                                                 >  2:45172/1‑150 (MQ=255)
                                                                                           ccGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTg                                                           <  2:1307001/150‑1 (MQ=255)
                                                                                           ccGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTg                                                           >  2:1480182/1‑150 (MQ=255)
                                                                                           ccGAGGGGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTg                                                           <  1:1911837/150‑1 (MQ=255)
                                                                                            cGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTgg                                                          >  1:1776833/1‑150 (MQ=255)
                                                                                                gcgcTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGcc                                                      >  2:1106611/1‑150 (MQ=255)
                                                                                                   cTGTACGTGTCCGGCCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGAt                                                   <  1:1132299/150‑1 (MQ=255)
                                                                                                    tGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCTC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATg                                                  <  2:195875/150‑1 (MQ=255)
                                                                                                     gTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATgg                                                 <  2:1659982/150‑1 (MQ=255)
                                                                                                       aCGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCA‑CGCAGGGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGcc                                               >  1:497076/1‑150 (MQ=255)
                                                                                                        cGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCgcgc                                                                                                       >  2:555437/1‑94 (MQ=255)
                                                                                                        cGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCgcgc                                                                                                       <  1:555437/94‑1 (MQ=255)
                                                                                                         gtgtCCGGGCAAATGTCGCTGGAGGCCCAGTACCTAGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCa                                             >  2:832936/1‑150 (MQ=255)
                                                                                                            tCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCt                                          >  1:873127/1‑150 (MQ=255)
                                                                                                            tCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGGGGCTACAAGCTGGCGCTTGGCGCCGATGGCCAAcat                                          >  2:1350503/1‑148 (MQ=255)
                                                                                                               gggCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGgccagcgc‑ca                                                                                   >  2:261859/1‑106 (MQ=255)
                                                                                                               gggCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGgccagcgc‑ca                                                                                   <  1:261859/106‑1 (MQ=255)
                                                                                                               gggCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGc                                       >  1:1847007/1‑150 (MQ=255)
                                                                                                               gggCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGc                                       >  1:936646/1‑150 (MQ=255)
                                                                                                                ggCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCa                                      <  1:1350503/150‑1 (MQ=255)
                                                                                                                ggCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCa                                      >  2:262494/1‑150 (MQ=255)
                                                                                                                   aaaTGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCTCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGtt                                   <  1:91949/150‑1 (MQ=255)
                                                                                                                       gTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCa                               <  1:262494/150‑1 (MQ=255)
                                                                                                                          gCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGTGGAAGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAggg                            >  1:1058761/1‑149 (MQ=255)
                                                                                                                              gAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTc                        <  1:11655/150‑1 (MQ=255)
                                                                                                                                   ccAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTg                                                           >  1:85657/1‑110 (MQ=255)
                                                                                                                                   ccAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTg                                                           <  2:85657/110‑1 (MQ=255)
                                                                                                                                   ccAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAg                     <  2:1756383/148‑1 (MQ=255)
                                                                                                                                   ccAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAg                     >  1:1756383/1‑148 (MQ=255)
                                                                                                                                   ccAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCa                   >  2:269978/1‑150 (MQ=255)
                                                                                                                                        aCCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGTCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCg              <  1:638062/150‑1 (MQ=255)
                                                                                                                                         cttGGCCAACTTGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGa             >  2:1288338/3‑150 (MQ=255)
                                                                                                                                         ccTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGa             <  1:1229015/150‑1 (MQ=255)
                                                                                                                                           tGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACg           >  1:334081/1‑150 (MQ=255)
                                                                                                                                               caacaaGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACGTAtt       >  1:1086905/1‑150 (MQ=255)
                                                                                                                                               caacaaGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACGTAtt       >  2:86599/1‑150 (MQ=255)
                                                                                                                                                aacaaGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCAc                  <  2:508503/138‑1 (MQ=255)
                                                                                                                                                aacaaGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCAc                  >  1:508503/1‑138 (MQ=255)
                                                                                                                                                aacaaGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACGTAttt      >  2:1822152/1‑150 (MQ=255)
                                                                                                                                                aacaaGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACGTAttt      >  1:817144/1‑150 (MQ=255)
                                                                                                                                                aacaaGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACGTAttt      >  1:1378888/1‑150 (MQ=255)
                                                                                                                                                   aaGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACGTATTTCTg   <  2:1776833/150‑1 (MQ=255)
                                                                                                                                                    aGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACGTATTTCTgg  <  1:984878/150‑1 (MQ=255)
                                                                                                                                                    aGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACGTATTTCTgg  <  2:936646/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
AGCCAGCCGCCAGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGC‑CAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACGTATTTCTGG  >  NC_002947/1899702‑1899999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑