Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,154,429	T→G	10.0%	Q129P (CAG→CCG)	PP_1909 ←	septum formation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,154,429	0	T	G	10.0%	208.3 / 16.0	80	Q129P (CAG→CCG)	PP_1909	septum formation protein
Reads supporting (aligned to +/- strand):  ref base T (41/31);  new base G (2/6);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01

CCGTGCGTGCTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGA  >  NC_002947/2154282‑2154577                                                                                                                                                     |                                                                                                                                                     ccGTGCGTGCTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCACTG‑CTCCGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTgg                                                                                                                                                    <  2:691146/150‑1 (MQ=255)    tgcgtgcTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCAt                                                                                                                                                 >  1:1048482/1‑150 (MQ=255)     gcgtgcTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCaat                                                                                                                                                <  2:1122237/150‑3 (MQ=255)     gcgtgcTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCaat                                                                                                                                                <  2:170296/150‑3 (MQ=255)       gtgcTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGc                                                                                                                                              <  1:362161/150‑1 (MQ=255)         gcTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGccc                                                                                                                                            >  1:13631/1‑150 (MQ=255)           tctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCgg                                                                                                                                          >  1:950863/1‑150 (MQ=255)           tctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCgg                                                                                                                                          >  2:1728863/1‑150 (MQ=255)           tctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCgg                                                                                                                                          >  2:519878/1‑150 (MQ=255)             tGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTg                                                                                                                                        <  2:859431/150‑1 (MQ=255)             tGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTg                                                                                                                                        <  1:1466814/150‑1 (MQ=255)             tGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAAAGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGGAGAAGGCACGCAATCCACCTGGCATTGCCCGGTg                                                                                                                                        >  1:1561631/1‑150 (MQ=255)              ggAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTgg                                                                                                                                       >  2:256231/1‑150 (MQ=255)               gAACAAGCTCCCCCCCCGCCCCTCCGCCTTGAATCTCCCTGCGCAATCCAGCGGCTTTTCTGCTGCTACGTAACGCTCGACCTGCTCCCTG‑TTCAGTTCACGCAGTGCCACGGTAAAAGGCACGCAATCCTCCTGGCATTGCCCGGTGgc                                                                                                                                      <  2:297824/150‑1 (MQ=255)               gAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGgc                                                                                                                                      >  2:982919/1‑150 (MQ=255)               gAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTgga                                                                                                                                      <  1:256231/150‑2 (MQ=255)                 aCAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGTAATGCCAGGTggatt                                                                                                                                    <  1:455359/150‑4 (MQ=255)                  cAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCt                                                                                                                                   >  2:1060279/1‑150 (MQ=255)                   aaGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCACTCCAGCGGCTGTCCCGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTg                                                                                                                                  >  2:1167641/1‑150 (MQ=255)                      cTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTc                                                                                                                               >  1:769932/1‑150 (MQ=255)                       tCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTca                                                                                                                              >  1:84578/1‑150 (MQ=255)                            cccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGt                                                                                                                         <  1:1089898/150‑1 (MQ=255)                            cccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGt                                                                                                                         <  2:1082396/150‑1 (MQ=255)                             ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTg                                                                                                                        >  1:1243654/1‑150 (MQ=255)                             ccAGCCCCTCCGCCTTGAAACTCACTGCGCAATACAGCGGCTGTTGTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCAAGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTg                                                                                                                        >  1:912768/1‑150 (MQ=255)                                gCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCa                                                                                                                     <  2:1118582/150‑1 (MQ=255)                                 ccccTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAg                                                                                                                    <  1:982919/150‑1 (MQ=255)                                          cTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAg                                                                                                           >  1:1142579/1‑150 (MQ=255)                                           ttGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGa                                                                                                          >  2:1318937/1‑150 (MQ=255)                                             gAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGaaa                                                                                                        <  2:1446212/150‑1 (MQ=255)                                              aaaCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAg                                                                                                       <  1:299421/150‑1 (MQ=255)                                              aaaCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGC‑CCCTGCCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAg                                                                                                       <  2:1698619/150‑1 (MQ=255)                                               aaCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGc                                                                                                      >  1:1233125/1‑150 (MQ=255)                                                aCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTaa                                                                                                            <  1:373238/143‑1 (MQ=255)                                                aCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTaa                                                                                                            >  2:373238/1‑143 (MQ=255)                                                aCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCt                                                                                                     <  2:13631/150‑1 (MQ=255)                                                  tCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCa                                                                                                   >  1:1089335/1‑150 (MQ=255)                                                  tCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCa                                                                                                   >  1:1688559/1‑150 (MQ=255)                                                   cccTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCAAGAAGGACCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCAc                                                                                                  <  1:1060279/150‑1 (MQ=255)                                                    ccTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCAcc                                                                                                 >  2:745250/1‑150 (MQ=255)                                                             tCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCg                                                                                        >  2:1889295/1‑150 (MQ=255)                                                                aGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCg                                                                                                                                           <  2:991811/96‑1 (MQ=255)                                                                aGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCg                                                                                                                                           >  1:991811/1‑96 (MQ=255)                                                                aGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTg                                                                                     <  1:523720/150‑1 (MQ=255)                                                                       gTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCa                                                                              <  2:950863/150‑1 (MQ=255)                                                                         tgtgcagcCACGTAGCGCTCGATCGCCTCCCTG‑CAAACATCACGCAGTGACACAATGATTAATACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCagt                                                                            <  2:912768/148‑1 (MQ=255)                                                                             ctgcCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACct                                                                                                >  1:1478490/1‑126 (MQ=255)                                                                             ctgcCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACct                                                                                                <  2:1478490/126‑1 (MQ=255)                                                                                ccACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCt                                                                     >  2:1218820/1‑150 (MQ=255)                                                                                 cACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTc                                                                    <  1:158897/150‑1 (MQ=255)                                                                                  aCGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGCAAACGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCg                                                                   >  2:367041/1‑150 (MQ=255)                                                                                       aCGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgc                                                              <  1:519878/150‑1 (MQ=255)                                                                                         gCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAg                                                                                                       >  2:4261/1‑107 (MQ=255)                                                                                         gCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAg                                                                                                       <  1:4261/107‑1 (MQ=255)                                                                                         gCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgcgc                                                            >  1:1474401/1‑150 (MQ=255)                                                                                          cTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgcgcg                                                           >  2:1316647/1‑150 (MQ=255)                                                                                                    tccctg‑ctcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTa                                                 >  1:1328888/1‑150 (MQ=255)                                                                                                       ctg‑ctcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGc                                              <  2:1233125/150‑1 (MQ=255)                                                                                                         g‑ctcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGaa                                                     >  1:1108203/1‑141 (MQ=255)                                                                                                         g‑ctcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGaa                                                     <  2:1108203/141‑1 (MQ=255)                                                                                                            tcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCt                                          >  2:1124732/1‑150 (MQ=255)                                                                                                                ttCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                      <  1:1167641/150‑1 (MQ=255)                                                                                                                ttCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                      >  2:619270/1‑150 (MQ=255)                                                                                                                  cACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCa                                    >  1:1125063/1‑150 (MQ=255)                                                                                                                   aCGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTc                                                                                <  2:1527793/105‑1 (MQ=255)                                                                                                                   aCGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTc                                                                                >  1:1527793/1‑105 (MQ=255)                                                                                                                      cAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCAc                                <  1:1318937/150‑1 (MQ=255)                                                                                                                          ttCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGc                            <  1:1506276/149‑1 (MQ=255)                                                                                                                            cACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTc                          <  1:1316647/150‑1 (MQ=255)                                                                                                                             aCGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTg                                                                                     >  2:450620/1‑90 (MQ=255)                                                                                                                             aCGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTg                                                                                     <  1:450620/90‑1 (MQ=255)                                                                                                                                      ggCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATAcc                >  2:6318/1‑150 (MQ=255)                                                                                                                                      ggCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATAcc                <  2:1561631/150‑1 (MQ=255)                                                                                                                                         aCGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGATCGCCCAATACCGCa             >  1:120622/1‑150 (MQ=255)                                                                                                                                             aaTCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCt         >  2:1673952/1‑150 (MQ=255)                                                                                                                                              aTCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTg        >  2:1206599/1‑150 (MQ=255)                                                                                                                                                 cACCGGGCAATGCCAGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgcgc                                                            <  2:1041785/95‑1 (MQ=255)                                                                                                                                                 cACCGGGCAATGCCAGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgcgc                                                            >  1:1041785/1‑95 (MQ=255)                                                                                                                                                  aCCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTcc    >  1:1802495/1‑150 (MQ=255)                                                                                                                                                    cTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCGCCTGCTCGCCCAATACCGCAACCTGGTCCGa  >  2:991743/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CCGTGCGTGCTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGA  >  NC_002947/2154282‑2154577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑