Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,154,434	T→A	7.3%	Q127H (CAA→CAT)	PP_1909 ←	septum formation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,154,434	0	T	A	7.3%	248.7 / 7.2	81	Q127H (CAA→CAT)	PP_1909	septum formation protein
Reads supporting (aligned to +/- strand):  ref base T (44/31);  new base A (2/4);  total (46/35)
Fisher's exact test for biased strand distribution p-value = 3.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGTGCTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCTA  >  NC_002947/2154285‑2154582                                                                                                                                                       |                                                                                                                                                     tgcgtgcTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCAt                                                                                                                                                      >  1:1048482/1‑150 (MQ=255)    gtgcTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGc                                                                                                                                                   <  1:362161/150‑1 (MQ=255)      gcTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGccc                                                                                                                                                 >  1:13631/1‑150 (MQ=255)        tctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCgg                                                                                                                                               >  2:519878/1‑150 (MQ=255)        tctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCgg                                                                                                                                               >  2:1728863/1‑150 (MQ=255)        tctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCgg                                                                                                                                               >  1:950863/1‑150 (MQ=255)          tGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTg                                                                                                                                             <  2:859431/150‑1 (MQ=255)          tGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTg                                                                                                                                             <  1:1466814/150‑1 (MQ=255)          tGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAAAGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGGAGAAGGCACGCAATCCACCTGGCATTGCCCGGTg                                                                                                                                             >  1:1561631/1‑150 (MQ=255)           ggAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTgg                                                                                                                                            >  2:256231/1‑150 (MQ=255)            gAACAAGCTCCCCCCCCGCCCCTCCGCCTTGAATCTCCCTGCGCAATCCAGCGGCTTTTCTGCTGCTACGTAACGCTCGACCTGCTCCCTG‑TTCAGTTCACGCAGTGCCACGGTAAAAGGCACGCAATCCTCCTGGCATTGCCCGGTGgc                                                                                                                                           <  2:297824/150‑1 (MQ=255)            gAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGgc                                                                                                                                           >  2:982919/1‑150 (MQ=255)            gAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGCAATGCCAGGTgga                                                                                                                                           <  1:256231/150‑2 (MQ=255)              aCAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCGGGTAATGCCAGGTggatt                                                                                                                                         <  1:455359/150‑4 (MQ=255)               cAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCt                                                                                                                                        >  2:1060279/1‑150 (MQ=255)                aaGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCACTCCAGCGGCTGTCCCGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTg                                                                                                                                       >  2:1167641/1‑150 (MQ=255)                   cTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTc                                                                                                                                    >  1:769932/1‑150 (MQ=255)                    tCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTca                                                                                                                                   >  1:84578/1‑150 (MQ=255)                         cccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGt                                                                                                                              <  2:1082396/150‑1 (MQ=255)                         cccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGt                                                                                                                              <  1:1089898/150‑1 (MQ=255)                          ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTg                                                                                                                             >  1:1243654/1‑150 (MQ=255)                          ccAGCCCCTCCGCCTTGAAACTCACTGCGCAATACAGCGGCTGTTGTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCAAGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTg                                                                                                                             >  1:912768/1‑150 (MQ=255)                             gCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCa                                                                                                                          <  2:1118582/150‑1 (MQ=255)                              ccccTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAg                                                                                                                         <  1:982919/150‑1 (MQ=255)                                       cTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAg                                                                                                                >  1:1142579/1‑150 (MQ=255)                                        ttGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGa                                                                                                               >  2:1318937/1‑150 (MQ=255)                                          gAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGaaa                                                                                                             <  2:1446212/150‑1 (MQ=255)                                           aaaCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAg                                                                                                            <  1:299421/150‑1 (MQ=255)                                           aaaCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGC‑CCCTGCCTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAg                                                                                                            <  2:1698619/150‑1 (MQ=255)                                            aaCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGc                                                                                                           >  1:1233125/1‑150 (MQ=255)                                             aCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTaa                                                                                                                 <  1:373238/143‑1 (MQ=255)                                             aCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTaa                                                                                                                 >  2:373238/1‑143 (MQ=255)                                             aCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCt                                                                                                          <  2:13631/150‑1 (MQ=255)                                               tCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCa                                                                                                        >  1:1688559/1‑150 (MQ=255)                                               tCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCa                                                                                                        >  1:1089335/1‑150 (MQ=255)                                                cccTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCAAGAAGGACCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCAc                                                                                                       <  1:1060279/150‑1 (MQ=255)                                                 ccTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCAcc                                                                                                      >  2:745250/1‑150 (MQ=255)                                                          tCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCg                                                                                             >  2:1889295/1‑150 (MQ=255)                                                             aGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCg                                                                                                                                                <  2:991811/96‑1 (MQ=255)                                                             aGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCg                                                                                                                                                >  1:991811/1‑96 (MQ=255)                                                             aGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTg                                                                                          <  1:523720/150‑1 (MQ=255)                                                                    gTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCa                                                                                   <  2:950863/150‑1 (MQ=255)                                                                      tgtgcagcCACGTAGCGCTCGATCGCCTCCCTG‑CAAACATCACGCAGTGACACAATGATTAATACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCagt                                                                                 <  2:912768/148‑1 (MQ=255)                                                                          ctgcCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACct                                                                                                     >  1:1478490/1‑126 (MQ=255)                                                                          ctgcCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACct                                                                                                     <  2:1478490/126‑1 (MQ=255)                                                                             ccACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCt                                                                          >  2:1218820/1‑150 (MQ=255)                                                                              cACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTc                                                                         <  1:158897/150‑1 (MQ=255)                                                                               aCGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGCAAACGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCg                                                                        >  2:367041/1‑150 (MQ=255)                                                                                    aCGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgc                                                                   <  1:519878/150‑1 (MQ=255)                                                                                      gCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAg                                                                                                            >  2:4261/1‑107 (MQ=255)                                                                                      gCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAg                                                                                                            <  1:4261/107‑1 (MQ=255)                                                                                      gCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgcgc                                                                 >  1:1474401/1‑150 (MQ=255)                                                                                       cTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgcgcg                                                                >  2:1316647/1‑150 (MQ=255)                                                                                                 tccctg‑ctcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTa                                                      >  1:1328888/1‑150 (MQ=255)                                                                                                    ctg‑ctcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGc                                                   <  2:1233125/150‑1 (MQ=255)                                                                                                      g‑ctcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGaa                                                          >  1:1108203/1‑141 (MQ=255)                                                                                                      g‑ctcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGaa                                                          <  2:1108203/141‑1 (MQ=255)                                                                                                         tcAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCt                                               >  2:1124732/1‑150 (MQ=255)                                                                                                             ttCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                           <  1:1167641/150‑1 (MQ=255)                                                                                                             ttCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                           >  2:619270/1‑150 (MQ=255)                                                                                                               cACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCa                                         >  1:1125063/1‑150 (MQ=255)                                                                                                                aCGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTc                                                                                     <  2:1527793/105‑1 (MQ=255)                                                                                                                aCGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTc                                                                                     >  1:1527793/1‑105 (MQ=255)                                                                                                                   cAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCAc                                     <  1:1318937/150‑1 (MQ=255)                                                                                                                       ttCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGc                                 <  1:1506276/149‑1 (MQ=255)                                                                                                                         cACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTc                               <  1:1316647/150‑1 (MQ=255)                                                                                                                          aCGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTg                                                                                          <  1:450620/90‑1 (MQ=255)                                                                                                                          aCGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTg                                                                                          >  2:450620/1‑90 (MQ=255)                                                                                                                                   ggCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATAcc                     <  2:1561631/150‑1 (MQ=255)                                                                                                                                   ggCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATAcc                     >  2:6318/1‑150 (MQ=255)                                                                                                                                      aCGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGATCGCCCAATACCGCa                  >  1:120622/1‑150 (MQ=255)                                                                                                                                          aaTCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCt              >  2:1673952/1‑150 (MQ=255)                                                                                                                                           aTCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTg             >  2:1206599/1‑150 (MQ=255)                                                                                                                                              cACCGGGCAATGCCAGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgcgc                                                                 <  2:1041785/95‑1 (MQ=255)                                                                                                                                              cACCGGGCAATGCCAGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgcgc                                                                 >  1:1041785/1‑95 (MQ=255)                                                                                                                                               aCCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTcc         >  1:1802495/1‑150 (MQ=255)                                                                                                                                                 cTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCGCCTGCTCGCCCAATACCGCAACCTGGTCCGa       >  2:991743/1‑150 (MQ=255)                                                                                                                                                     cATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCt   <  1:619270/150‑1 (MQ=255)                                                                                                                                                      aTTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCTa  >  1:1465375/1‑150 (MQ=255)                                                                                                                                                      aTTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCTa  >  1:54168/1‑150 (MQ=255)                                                                                                                                                      aTTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCTa  >  2:1281232/1‑150 (MQ=255)                                                                                                                                                      aTTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCTa  >  2:1112639/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                     TGCGTGCTCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTG‑CTCAGTTCACGCAGTGTCACGGTAAAAGGCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCTA  >  NC_002947/2154285‑2154582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑