Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,275,079 A→C 5.6% L152V (TTG→GTG)  PP_2005 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,275,0790AC 5.6% 191.0 / 4.2 71L152V (TTG→GTG) PP_2005hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (34/33);  new base C (2/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

CGGGCCACGGCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCAG  >  NC_002947/2274930‑2275227
                                                                                                                                                     |                                                                                                                                                    
cGGGCCACGGCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCaa                                                                                                                                                      <  1:1904538/150‑1 (MQ=255)
  ggCCACGGCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGt                                                                                                                                                    >  1:384210/1‑150 (MQ=255)
    ccACGGCCAGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTgc                                                                                                                                                  <  2:1046185/150‑1 (MQ=255)
     cACGGCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTgcg                                                                                                                                                 >  1:1084704/1‑150 (MQ=255)
     cACGGCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTgcg                                                                                                                                                 >  1:1099860/1‑150 (MQ=255)
             gTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCACTTGCGCAtgaagg                                                                                                                                         <  2:1088659/150‑6 (MQ=255)
               aTGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGgcgc                                                                                                                                        <  1:139349/149‑1 (MQ=255)
               aTGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGgcgc                                                                                                                                        >  2:139349/1‑149 (MQ=255)
                     tACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAgtc                                                                                                                                 <  1:355646/150‑1 (MQ=255)
                          cTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGc                                                                                                                            >  2:808214/1‑150 (MQ=255)
                          cTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGc                                                                                                                            <  2:1671330/150‑1 (MQ=255)
                               ttCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCa                                                                                                                       <  1:1236496/150‑1 (MQ=255)
                               ttCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCa                                                                                                                       <  2:384515/150‑1 (MQ=255)
                                         gTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCCGCCTTCATGCGCAAGCGCTCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGaa                                                                                                             <  2:466552/150‑1 (MQ=255)
                                              ggCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACcgcg                                                                                                        <  2:794069/150‑1 (MQ=255)
                                              ggCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGAACCTGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACcgcg                                                                                                        >  1:99867/1‑150 (MQ=255)
                                               gcagcaCGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGa                                                                                                       <  1:616748/150‑1 (MQ=255)
                                                   caCGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGACCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAg                                                                                                      <  1:1741478/147‑1 (MQ=255)
                                                   caCGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGACCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAg                                                                                                      >  2:1741478/1‑147 (MQ=255)
                                                               tgCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCa                                                                                                                                               <  1:425444/93‑1 (MQ=255)
                                                               ggCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCa                                                                                                                                               >  2:425444/1‑94 (MQ=255)
                                                               ggCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCgg                                                                                       <  2:469035/150‑1 (MQ=255)
                                                               ggCCACCAAGGTGACGCCAAAGTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCgg                                                                                       <  1:165587/150‑1 (MQ=255)
                                                                gCCACCAAGGTGACGCCAAATTCCCTGGGGAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCggg                                                                                      >  1:49833/1‑150 (MQ=255)
                                                                gCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCCTCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGCACCGCGAGGCCATCGCGCAGCggg                                                                                      >  2:4524/1‑150 (MQ=255)
                                                                gCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCggg                                                                                      <  1:1909865/150‑1 (MQ=255)
                                                                 ccaccaAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGt                                                                                     <  1:1337550/150‑1 (MQ=255)
                                                                  caccaAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGtt                                                                                    >  1:557392/1‑150 (MQ=255)
                                                                     caAGGTGACGCCAAATTCCCTGGCCAGGCCGTCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGATCCGCGAGGCCATAGCGCAGCGGGTTGCt                                                                                 <  2:996188/150‑1 (MQ=255)
                                                                     caAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCt                                                                                 <  2:384210/150‑1 (MQ=255)
                                                                        ggTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAg                                                                              <  1:655081/150‑1 (MQ=255)
                                                                             cGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCa                                                                         <  1:1877588/150‑1 (MQ=255)
                                                                              gCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCgg                                                                                       <  2:419323/135‑1 (MQ=255)
                                                                              gCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCgg                                                                                       >  1:419323/1‑135 (MQ=255)
                                                                              gCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAg                                                                        >  1:914893/1‑150 (MQ=255)
                                                                              gCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAg                                                                        >  2:1504645/1‑150 (MQ=255)
                                                                               ccAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTTCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGc                                                                       >  1:1294509/1‑150 (MQ=255)
                                                                               ccAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCCGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGc                                                                       >  2:1560596/1‑150 (MQ=255)
                                                                                cAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCACTTGCGCAtgaag                                                                                                                                          <  1:1382600/82‑5 (MQ=255)
                                                                                cAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCACTTGCGCAtgaag                                                                                                                                          >  2:1382600/1‑78 (MQ=255)
                                                                                cAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGcc                                                                      <  2:147937/150‑1 (MQ=255)
                                                                                     tCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGt                                                                 <  1:808214/150‑1 (MQ=255)
                                                                                      cccTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTc                                                                >  2:1684351/1‑150 (MQ=255)
                                                                                       ccTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAAc                                                                                                            >  1:1211609/1‑105 (MQ=255)
                                                                                       ccTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAAc                                                                                                            >  1:1211809/1‑105 (MQ=255)
                                                                                       ccTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAAc                                                                                                            <  2:1211809/105‑1 (MQ=255)
                                                                                       ccTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAAc                                                                                                            <  2:1211609/105‑1 (MQ=255)
                                                                                        cTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCgg                                                              >  1:1622759/1‑150 (MQ=255)
                                                                                             cAGTCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGGCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTc                                                         <  1:821255/150‑1 (MQ=255)
                                                                                              aGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCTGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCg                                                        <  1:682367/150‑1 (MQ=255)
                                                                                              aGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTGCCAGCCATTGTCGGGCTTCg                                                        <  2:819098/150‑1 (MQ=255)
                                                                                                gccgccGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTg                                                      >  2:868748/1‑150 (MQ=255)
                                                                                                       aaCAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTgg                                               <  1:636489/150‑1 (MQ=255)
                                                                                                               ctgGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTg                                       >  2:1364845/1‑150 (MQ=255)
                                                                                                                tgGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGa                                      <  1:52426/150‑1 (MQ=255)
                                                                                                                tgGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGa                                      <  2:752794/150‑1 (MQ=255)
                                                                                                                  gTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAATGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCCAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCGTTCGGTGACTTGATa                                    >  2:82455/1‑150 (MQ=255)
                                                                                                                     gTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATAccg                                 >  2:373358/1‑148 (MQ=255)
                                                                                                                        tGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTc                              >  1:1794195/1‑150 (MQ=255)
                                                                                                                          cGGCCAACTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGt                            >  2:471681/1‑150 (MQ=255)
                                                                                                                           ggCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGtt                           <  1:868748/150‑1 (MQ=255)
                                                                                                                           ggCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGtt                           >  1:1499220/1‑150 (MQ=255)
                                                                                                                           ggCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTAGTGGCTTTGCGTGACTTGATACAGCTCGtt                           >  1:794194/1‑150 (MQ=255)
                                                                                                                                tCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTtcttct                      >  1:452638/1‑150 (MQ=255)
                                                                                                                                    ctgggCCTTCATGCGCAAGGGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGACCCGCGAGGCCATAGCGCAGCGGGTTGATCAATTTGTGCCATTGTCGGGCTTCGTGGCTTTGGGTTACTTGTTACAGCTCGTTCTTCTCTcc                  <  2:259328/147‑1 (MQ=255)
                                                                                                                                     tCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCcg                 >  2:484195/1‑150 (MQ=255)
                                                                                                                                       ggCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCcgcg               >  2:685031/1‑150 (MQ=255)
                                                                                                                                          cTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCCGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCgcgcgc            >  1:299429/1‑150 (MQ=255)
                                                                                                                                          cTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCgcgcgc            >  1:123661/1‑150 (MQ=255)
                                                                                                                                              aTGCGCACTTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCcac        >  2:1672618/1‑150 (MQ=255)
                                                                                                                                              aTGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCcac        >  2:1703344/1‑150 (MQ=255)
                                                                                                                                                   cAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCATGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCa   <  1:1260515/150‑1 (MQ=255)
                                                                                                                                                    aaGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCTGTTCTTTCTATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACTGCCAg  <  2:387847/150‑1 (MQ=255)
                                                                                                                                                    aaGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCAg  <  1:76530/150‑1 (MQ=255)
                                                                                                                                                    aaGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCAg  <  1:471681/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGGGCCACGGCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCAG  >  NC_002947/2274930‑2275227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑