Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,275,080 G→T 5.4% H151Q (CAC→CAA)  PP_2005 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,275,0800GT 5.4% 235.8 / 2.0 74H151Q (CAC→CAA) PP_2005hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (33/36);  new base T (2/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

GGCCACGGCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCAG  >  NC_002947/2274932‑2275227
                                                                                                                                                    |                                                                                                                                                   
ggCCACGGCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGt                                                                                                                                                    >  1:384210/1‑150 (MQ=255)
  ccACGGCCAGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTgc                                                                                                                                                  <  2:1046185/150‑1 (MQ=255)
   cACGGCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTgcg                                                                                                                                                 >  1:1099860/1‑150 (MQ=255)
   cACGGCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTgcg                                                                                                                                                 >  1:1084704/1‑150 (MQ=255)
           gTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCACTTGCGCAtgaagg                                                                                                                                         <  2:1088659/150‑6 (MQ=255)
             aTGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGgcgc                                                                                                                                        >  2:139349/1‑149 (MQ=255)
             aTGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGgcgc                                                                                                                                        <  1:139349/149‑1 (MQ=255)
                   tACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAgtc                                                                                                                                 <  1:355646/150‑1 (MQ=255)
                        cTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGc                                                                                                                            <  2:1671330/150‑1 (MQ=255)
                        cTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGc                                                                                                                            >  2:808214/1‑150 (MQ=255)
                             ttCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCa                                                                                                                       <  2:384515/150‑1 (MQ=255)
                             ttCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCa                                                                                                                       <  1:1236496/150‑1 (MQ=255)
                                       gTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCCGCCTTCATGCGCAAGCGCTCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGaa                                                                                                             <  2:466552/150‑1 (MQ=255)
                                            ggCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACcgcg                                                                                                        <  2:794069/150‑1 (MQ=255)
                                            ggCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGAACCTGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACcgcg                                                                                                        >  1:99867/1‑150 (MQ=255)
                                             gcagcaCGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGa                                                                                                       <  1:616748/150‑1 (MQ=255)
                                                 caCGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGACCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAg                                                                                                      <  1:1741478/147‑1 (MQ=255)
                                                 caCGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGACCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAg                                                                                                      >  2:1741478/1‑147 (MQ=255)
                                                             tgCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCa                                                                                                                                               <  1:425444/93‑1 (MQ=255)
                                                             ggCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCa                                                                                                                                               >  2:425444/1‑94 (MQ=255)
                                                             ggCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCgg                                                                                       <  2:469035/150‑1 (MQ=255)
                                                             ggCCACCAAGGTGACGCCAAAGTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCgg                                                                                       <  1:165587/150‑1 (MQ=255)
                                                              gCCACCAAGGTGACGCCAAATTCCCTGGGGAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCggg                                                                                      >  1:49833/1‑150 (MQ=255)
                                                              gCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCCTCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGCACCGCGAGGCCATCGCGCAGCggg                                                                                      >  2:4524/1‑150 (MQ=255)
                                                              gCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCggg                                                                                      <  1:1909865/150‑1 (MQ=255)
                                                               ccaccaAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGt                                                                                     <  1:1337550/150‑1 (MQ=255)
                                                                caccaAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGtt                                                                                    >  1:557392/1‑150 (MQ=255)
                                                                   caAGGTGACGCCAAATTCCCTGGCCAGGCCGTCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGATCCGCGAGGCCATAGCGCAGCGGGTTGCt                                                                                 <  2:996188/150‑1 (MQ=255)
                                                                   caAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCt                                                                                 <  2:384210/150‑1 (MQ=255)
                                                                      ggTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAg                                                                              <  1:655081/150‑1 (MQ=255)
                                                                           cGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCa                                                                         <  1:1877588/150‑1 (MQ=255)
                                                                            gCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCgg                                                                                       <  2:419323/135‑1 (MQ=255)
                                                                            gCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCgg                                                                                       >  1:419323/1‑135 (MQ=255)
                                                                            gCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAg                                                                        >  1:914893/1‑150 (MQ=255)
                                                                            gCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAg                                                                        >  2:1504645/1‑150 (MQ=255)
                                                                             ccAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTTCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGc                                                                       >  1:1294509/1‑150 (MQ=255)
                                                                             ccAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCCGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGc                                                                       >  2:1560596/1‑150 (MQ=255)
                                                                              cAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCACTTGCGCAtgaag                                                                                                                                          <  1:1382600/82‑5 (MQ=255)
                                                                              cAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCACTTGCGCAtgaag                                                                                                                                          >  2:1382600/1‑78 (MQ=255)
                                                                              cAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGcc                                                                      <  2:147937/150‑1 (MQ=255)
                                                                                   tCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGt                                                                 <  1:808214/150‑1 (MQ=255)
                                                                                    cccTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTc                                                                >  2:1684351/1‑150 (MQ=255)
                                                                                     ccTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAAc                                                                                                            >  1:1211609/1‑105 (MQ=255)
                                                                                     ccTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAAc                                                                                                            >  1:1211809/1‑105 (MQ=255)
                                                                                     ccTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAAc                                                                                                            <  2:1211809/105‑1 (MQ=255)
                                                                                     ccTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAAc                                                                                                            <  2:1211609/105‑1 (MQ=255)
                                                                                      cTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCgg                                                              >  1:1622759/1‑150 (MQ=255)
                                                                                           cAGTCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGGCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTc                                                         <  1:821255/150‑1 (MQ=255)
                                                                                            aGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCTGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCg                                                        <  1:682367/150‑1 (MQ=255)
                                                                                            aGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTGCCAGCCATTGTCGGGCTTCg                                                        <  2:819098/150‑1 (MQ=255)
                                                                                              gccgccGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTg                                                      >  2:868748/1‑150 (MQ=255)
                                                                                                     aaCAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTgg                                               <  1:636489/150‑1 (MQ=255)
                                                                                                             ctgGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTg                                       >  2:1364845/1‑150 (MQ=255)
                                                                                                              tgGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGa                                      <  1:52426/150‑1 (MQ=255)
                                                                                                              tgGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGa                                      <  2:752794/150‑1 (MQ=255)
                                                                                                                gTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAATGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCCAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCGTTCGGTGACTTGATa                                    >  2:82455/1‑150 (MQ=255)
                                                                                                                   gTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATAccg                                 >  2:373358/1‑148 (MQ=255)
                                                                                                                      tGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTc                              >  1:1794195/1‑150 (MQ=255)
                                                                                                                        cGGCCAACTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGt                            >  2:471681/1‑150 (MQ=255)
                                                                                                                         ggCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGtt                           <  1:868748/150‑1 (MQ=255)
                                                                                                                         ggCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGtt                           >  1:1499220/1‑150 (MQ=255)
                                                                                                                         ggCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTAGTGGCTTTGCGTGACTTGATACAGCTCGtt                           >  1:794194/1‑150 (MQ=255)
                                                                                                                              tCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTtcttct                      >  1:452638/1‑150 (MQ=255)
                                                                                                                                  ctgggCCTTCATGCGCAAGGGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGACCCGCGAGGCCATAGCGCAGCGGGTTGATCAATTTGTGCCATTGTCGGGCTTCGTGGCTTTGGGTTACTTGTTACAGCTCGTTCTTCTCTcc                  <  2:259328/147‑1 (MQ=255)
                                                                                                                                   tCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCcg                 >  2:484195/1‑150 (MQ=255)
                                                                                                                                     ggCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCcgcg               >  2:685031/1‑150 (MQ=255)
                                                                                                                                        cTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCCGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCgcgcgc            >  1:299429/1‑150 (MQ=255)
                                                                                                                                        cTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCgcgcgc            >  1:123661/1‑150 (MQ=255)
                                                                                                                                            aTGCGCACTTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCcac        >  2:1672618/1‑150 (MQ=255)
                                                                                                                                            aTGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCcac        >  2:1703344/1‑150 (MQ=255)
                                                                                                                                                 cAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCATGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCa   <  1:1260515/150‑1 (MQ=255)
                                                                                                                                                  aaGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCTGTTCTTTCTATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACTGCCAg  <  2:387847/150‑1 (MQ=255)
                                                                                                                                                  aaGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCAg  <  1:76530/150‑1 (MQ=255)
                                                                                                                                                  aaGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCAg  <  1:471681/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCCACGGCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCAG  >  NC_002947/2274932‑2275227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑