Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,540,356 G→A 5.3% Q20Q (CAG→CAA)  PP_2232 → XRE family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,540,3560GA 5.3% 224.3 / 2.3 76Q20Q (CAG→CAA) PP_2232XRE family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (34/38);  new base A (2/2);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

ACAATCGCCGCAAAAGTGGGCAGTATACTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTGGGCGTGGCTTTCAGCACCCTGATCCAGGCGCCAGACCT  >  NC_002947/2540211‑2540502
                                                                                                                                                 |                                                                                                                                                  
acaATCGCCGCAAAAGTGGGCAGTATACTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAggcgg                                                                                                                                                >  1:480982/1‑150 (MQ=255)
   aTCGCCGCAAAAGTGGGCAGTATACTTCGCAATCTTGCCCAGTGAGCTATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGgcgc                                                                                                                                             <  1:1450993/150‑1 (MQ=255)
    tCGCCGCAAAAGTGGGCAGTATACTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCt                                                                                                                                            >  2:1842760/1‑150 (MQ=255)
      gccgcAAAAGTGGGCAGTATACTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCACCGCCGCCGGCTTGAGTCAGGCGGCGCTgg                                                                                                                                          >  2:514303/1‑150 (MQ=255)
         gcAAAAGTGGGCAGTATACTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCg                                                                                                                                       >  1:1230930/1‑150 (MQ=255)
              aGTGGGCAGTATACTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGt                                                                                                                                  <  2:1671390/150‑1 (MQ=255)
                  ggCAGTATACTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAg                                                                                                                              <  2:516039/150‑1 (MQ=255)
                  ggCAGTATACTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAg                                                                                                                              <  1:1459920/150‑1 (MQ=255)
                  ggCAGTATACTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAg                                                                                                                              >  2:1306312/1‑150 (MQ=255)
                   gCAGTATACTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCTCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAgg                                                                                                                             <  2:202356/150‑1 (MQ=255)
                     aGTATACTGCGCAACCTTGACCAGTGAGCAATATAGTGCACAAGGATGCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGACTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAgggg                                                                                                                           <  1:727686/150‑1 (MQ=255)
                      gTATACTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCCTCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAggggg                                                                                                                          >  1:959388/1‑149 (MQ=255)
                       tataCTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGgcgc                                                                                                                                             <  2:315663/130‑1 (MQ=255)
                       tataCTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGgcgc                                                                                                                                             >  1:315663/1‑130 (MQ=255)
                       tataCTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTc                                                                                                                         >  2:1056087/1‑150 (MQ=255)
                            tGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCg                                                                                                                    >  2:1046498/1‑150 (MQ=255)
                              cgcAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGgc                                                                                                                  <  1:1056087/150‑1 (MQ=255)
                                cAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGgcgc                                                                                                                >  2:1313996/1‑150 (MQ=255)
                                cAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAAGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGgcgc                                                                                                                >  2:1427927/1‑150 (MQ=255)
                                cAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAAGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGgcgc                                                                                                                >  2:1429511/1‑150 (MQ=255)
                                   tCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGc                                                                                                                                         >  2:62856/1‑122 (MQ=255)
                                   tCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGc                                                                                                                                         <  1:62856/122‑1 (MQ=255)
                                   tCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATg                                                                                                             >  2:179890/1‑150 (MQ=255)
                                        aCCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCCAGCCATCCCGGTTGAGCCGGCGCATGCTGGt                                                                                                        <  2:1606990/150‑1 (MQ=255)
                                         ccAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTc                                                                                                       >  1:1364831/1‑150 (MQ=255)
                                          cAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTcg                                                                                                      >  2:908735/1‑150 (MQ=255)
                                             tGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGa                                                                                                   <  1:1842760/150‑1 (MQ=255)
                                             tGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGa                                                                                                   <  1:1046498/150‑1 (MQ=255)
                                                   atatAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAgg                                                                                             <  2:62057/150‑1 (MQ=255)
                                                        gTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAggcgggc                                                                                        <  1:1601511/150‑1 (MQ=255)
                                                         tGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAggc                                                                                            <  2:338193/145‑1 (MQ=255)
                                                         tGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAggc                                                                                            >  1:338193/1‑145 (MQ=255)
                                                          gCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCga                                                                                      <  2:131986/150‑1 (MQ=255)
                                                            acaAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAgg                                                                                                                             >  1:618107/1‑109 (MQ=255)
                                                            acaAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAgg                                                                                                                             <  2:618107/109‑1 (MQ=255)
                                                              aaGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGagaag                                                                                  <  1:1313996/150‑1 (MQ=255)
                                                                ggATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAgaagaa                                                                                <  1:179890/150‑1 (MQ=255)
                                                                      ttcGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAg                                                                          >  2:1375825/1‑150 (MQ=255)
                                                                         gCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATg                                                                                                             >  1:1515272/1‑112 (MQ=255)
                                                                         gCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATg                                                                                                             <  2:1515272/112‑1 (MQ=255)
                                                                         gCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCt                                                                       >  2:419654/1‑150 (MQ=255)
                                                                            ccGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGAc                                                                    <  2:480982/150‑1 (MQ=255)
                                                                                 cATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACAt                                                               >  2:254489/1‑150 (MQ=255)
                                                                                   tCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTg                                                             <  2:1675497/150‑1 (MQ=255)
                                                                                    cGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTgg                                                            <  1:460050/150‑1 (MQ=255)
                                                                                      gTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGAc                                                          >  1:1853889/1‑150 (MQ=255)
                                                                                      gTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGAGACTAGAATGTCAGCCTGACCACATTGGAc                                                          <  1:1401576/150‑1 (MQ=255)
                                                                                       tgctgcAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAAt                                                                               >  2:1106371/1‑128 (MQ=255)
                                                                                       tgctgcAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAAt                                                                               <  1:1106371/128‑1 (MQ=255)
                                                                                          tgcAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGa                                                      >  1:601715/1‑150 (MQ=255)
                                                                                            cAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGAtt                                                    >  2:1128152/1‑150 (MQ=255)
                                                                                              aCATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGAcc                                                         <  1:529951/143‑1 (MQ=255)
                                                                                              aCATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGAcc                                                         >  2:529951/1‑143 (MQ=255)
                                                                                                  gTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATCGGGCCGGAGTGCCGaa                                              >  2:987186/1‑150 (MQ=255)
                                                                                                    cAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATATAAGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGc                                            <  1:1079309/150‑1 (MQ=255)
                                                                                                         tCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTgg                                       <  2:601715/150‑1 (MQ=255)
                                                                                                         tCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAAGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTgg                                       <  1:1429511/150‑1 (MQ=255)
                                                                                                         tCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAAGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTgg                                       <  1:1427927/150‑1 (MQ=255)
                                                                                                            aTGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGt                                                                             <  1:1409645/109‑1 (MQ=255)
                                                                                                            aTGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGt                                                                             >  2:1409645/1‑109 (MQ=255)
                                                                                                              ttgCGCAGACTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGc                                                                         >  2:496501/2‑111 (MQ=255)
                                                                                                              ttgCGCAGACTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGc                                                                         <  1:496501/110‑1 (MQ=255)
                                                                                                               tgCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTGGGCGTgg                                 <  1:908735/150‑1 (MQ=255)
                                                                                                                    aGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGGGTCACGGGCTTGTGGCAGCCGGCGCATGCTGACGGTGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTGGGCGTGGCTTTc                            <  1:1375825/150‑1 (MQ=255)
                                                                                                                     gTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTGTTTTGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTGGGCGTGGCTTTCa                           <  1:19985/150‑1 (MQ=255)
                                                                                                                        tGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTGGGCGTGGCTTTCAGCa                        <  2:1364831/150‑1 (MQ=255)
                                                                                                                           gcAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTGGGCGTGGCTTTCAGCAccc                     >  1:726773/1‑150 (MQ=255)
                                                                                                                            cAACGCCGCCGGCTTTAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGa                                                           <  1:968264/111‑1 (MQ=255)
                                                                                                                            cAACGCCGCCGGCTTTAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGa                                                           >  2:968264/1‑111 (MQ=255)
                                                                                                                                   gccgGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTGGGCGTGGCTTTCAGCACCCTGATCCAg             <  1:716742/150‑1 (MQ=255)
                                                                                                                                    ccgGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTGGGCGTGGCTTTCAGCACCCTGATCCAgg            <  1:419654/150‑1 (MQ=255)
                                                                                                                                         ttGAGTCAGGCGGCGCTGGCCGAGCGTTCATGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTGGGCGTGGCTTTCAGCACCCTGATCCAGGCGCCa       >  2:1779923/1‑150 (MQ=255)
                                                                                                                                             gTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTGGGCGTGGCTTTCAGCACCCTGATCCAGGCGCCAGAcc   >  2:1912912/1‑150 (MQ=255)
                                                                                                                                              tCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGTCGAAGCCTTGGGCGTGGCTTTCa                           <  1:1071146/125‑1 (MQ=255)
                                                                                                                                              tCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGTCGAAGCCTTGGGCGTGGCTTTCa                           >  2:1071146/1‑125 (MQ=255)
                                                                                                                                              tCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTGGGCGTGGCTTTCAGCACCCTGATCCAGGCGCCAGACCt  >  1:983173/1‑150 (MQ=255)
                                                                                                                                              tCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTGGGCGTGGCTTTCAGCACCCTGATCCAGGCGCCAGACCt  >  2:972822/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ACAATCGCCGCAAAAGTGGGCAGTATACTGCGCAATCTTGACCAGTGAGCAATATAGTGCACAAGGATTCTTCGCACCGGGCATCGGTGCTGCAACATGTCAGCCTCAATGTGCGCAGTCTGCGCAACGCCGCCGGCTTGAGTCAGGCGGCGCTGGCCGAGCGTTCAGGGGTCAGCCGGCGCATGCTGGTCGCGATCGAGGCGGGCGAGAAGAATGTCAGCCTGACCACATTGGACCTGATTGCCGAAGCCTTGGGCGTGGCTTTCAGCACCCTGATCCAGGCGCCAGACCT  >  NC_002947/2540211‑2540502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑