Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,650,204 A→T 7.2% intergenic (+100/+34) PP_2320 → / ← oprI hypothetical protein/major outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,650,2040AT 7.2% 153.4 / 4.5 56intergenic (+100/+34)PP_2320/oprIhypothetical protein/major outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (26/26);  new base T (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01

ACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCTTC  >  NC_002947/2650055‑2650351
                                                                                                                                                     |                                                                                                                                                   
aCCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACaa                                                                                                                                                     >  2:601785/1‑150 (MQ=255)
    gggggCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCTCTTGCAAAGGCAATAAAAAAGCCGACCCACAAGTgg                                                                                                                                                 <  1:833043/150‑1 (MQ=255)
        gCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCg                                                                                                                                             <  1:1826319/150‑1 (MQ=255)
          cGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGc                                                                                                                                           <  1:730253/150‑1 (MQ=255)
          cGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGc                                                                                                                                           <  1:694517/150‑1 (MQ=255)
           gCACCGATGGTGACGAGCATTCCGCCCGAACTGAAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACAGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGCGGGTCGGct                                                                                                                                          >  2:706252/1‑150 (MQ=255)
                 aTGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTaa                                                                                                                                    <  1:1246516/150‑1 (MQ=255)
                        cGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCg                                                                                                                             >  2:859594/1‑150 (MQ=255)
                           gCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGa                                                                                                                          <  1:1450974/150‑1 (MQ=255)
                                              gTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAATGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctg                                                                                                       >  2:1838143/1‑150 (MQ=255)
                                                aaGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctggc                                                                                                     <  2:386794/150‑1 (MQ=255)
                                                    tAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAACGCAATAAAAAAGCCGACCCACAAGTGGGTCAGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTg                                                                                                 >  2:721513/1‑150 (MQ=255)
                                                                  ccAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACATGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAg                                                                                   >  1:719238/1‑150 (MQ=255)
                                                                    aGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGGTGATTACTTGCGGCTGGCTTTGTCCAGCATACGCAggg                                                                                 >  1:16855/1‑150 (MQ=255)
                                                                      cccTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGgcg                                                                               <  1:597393/149‑1 (MQ=255)
                                                                            ccTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGct                                                                                                                                          >  2:1519691/1‑85 (MQ=255)
                                                                            ccTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGct                                                                                                                                          <  1:1519691/85‑1 (MQ=255)
                                                                               tAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTg                                                                      <  1:1711270/150‑1 (MQ=255)
                                                                                  tGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctgg                                                                                                      <  1:297721/115‑1 (MQ=255)
                                                                                  tGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctgg                                                                                                      >  2:297721/1‑115 (MQ=255)
                                                                                  tGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGACGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCt                                                                   <  2:1079699/150‑1 (MQ=255)
                                                                                       gCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGa                                                                                                                       >  2:15292/1‑93 (MQ=255)
                                                                                       gCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGa                                                                                                                       <  1:15292/93‑1 (MQ=255)
                                                                                         cTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAg                                                            <  1:601785/150‑1 (MQ=255)
                                                                                                tACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTctg                                                     >  1:750032/1‑150 (MQ=255)
                                                                                                   ctgctgACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTctgctg                                                  >  2:43584/1‑150 (MQ=255)
                                                                                                      ctgACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGc                                               <  1:859594/150‑1 (MQ=255)
                                                                                                         aCCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCtt                                            <  2:750032/150‑1 (MQ=255)
                                                                                                           cgcgTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCt                                          >  2:307808/1‑150 (MQ=255)
                                                                                                           cgcgTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCt                                          >  2:13320/1‑150 (MQ=255)
                                                                                                              gTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGTGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAg                                       >  1:1186294/1‑150 (MQ=255)
                                                                                                              gTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGc                                                                                                            >  2:831246/1‑81 (MQ=255)
                                                                                                              gTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGc                                                                                                            <  1:831246/81‑1 (MQ=255)
                                                                                                              gTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAg                                       <  1:1079644/150‑1 (MQ=255)
                                                                                                                  gCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGcttc                                                                                                                                        <  2:912434/49‑1 (MQ=255)
                                                                                                                  gCCGGTTTCAAAGGCAATAAAAAAGCCGACCCAAAAGTGGGTCGGcttc                                                                                                                                        >  1:912434/1‑49 (MQ=255)
                                                                                                                   ccGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGtt                                                                       >  2:1205416/1‑113 (MQ=255)
                                                                                                                   ccGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGtt                                                                       <  1:1205416/113‑1 (MQ=255)
                                                                                                                   ccGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCt                                  >  1:1632751/1‑150 (MQ=255)
                                                                                                                        ttCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCACTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCAt                             >  1:709062/1‑150 (MQ=255)
                                                                                                                           aaaGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGc                          >  2:132556/1‑150 (MQ=255)
                                                                                                                                 aaTAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCgtcgtc                    >  1:369398/1‑150 (MQ=255)
                                                                                                                                     aaaaaGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGcc                <  2:1103779/150‑1 (MQ=255)
                                                                                                                                     aaaaaGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGcc                <  1:132556/150‑1 (MQ=255)
                                                                                                                                      aaaaGCCGACCCACAAGTGGATCGGCTTCTTAAGAATCCGGGTGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCt               >  1:982594/1‑150 (MQ=255)
                                                                                                                                       gaaGCCGACCCACTTGTGGGTCGGCTTCTTAACAATCCGTGAGGAGTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCtt              >  1:441882/2‑150 (MQ=255)
                                                                                                                                          gCCGACCCACTTGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTct                                                      >  1:1521054/1‑107 (MQ=255)
                                                                                                                                          gCCGACCCACTTGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTct                                                      <  2:1521054/107‑1 (MQ=255)
                                                                                                                                            cGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGt         >  1:138769/1‑150 (MQ=255)
                                                                                                                                             gACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTa        >  1:1640208/1‑150 (MQ=255)
                                                                                                                                             gACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTa        <  1:103165/150‑1 (MQ=255)
                                                                                                                                              aCCCACTTGTGGGTCGGCTTCTTAACAATCCGTGa                                                                                                                          <  1:257152/35‑1 (MQ=18)
                                                                                                                                              aCCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCtt                                            >  1:1782968/1‑113 (MQ=255)
                                                                                                                                              aCCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCtt                                            <  2:1782968/113‑1 (MQ=255)
                                                                                                                                                  acaAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTc                                                                                               <  2:7936/58‑1 (MQ=255)
                                                                                                                                                  acaAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTc                                                                                               >  1:7936/1‑58 (MQ=255)
                                                                                                                                                   caAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCTTc  <  1:583171/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCTTC  >  NC_002947/2650055‑2650351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑