Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,658,815 G→A 6.7% A10V (GCC→GTC)  PP_2330 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,658,8150GA 6.7% 170.9 / 2.8 60A10V (GCC→GTC) PP_2330hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/24);  new base A (2/2);  total (34/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

CATTTCCGGCACATGGATGCCGGCCGCCAGTGCGCGCTCCTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAGCCCACCAGGATCAGCAGCACCGCCGGGAAGGTG  >  NC_002947/2658667‑2658963
                                                                                                                                                    |                                                                                                                                                    
cATTTCCGGCACATGGATGCCGGCCGCCAGTGCGCGCTCCTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGc                                                                                                                                                     >  2:1173341/1‑150 (MQ=255)
      cGGCACATGGATGCCGGCCGCCAGTGCGCGCTCCTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCa                                                                                                                                               >  1:1692417/1‑150 (MQ=255)
       ggCACATGGATGCCGGCCGCCAGTGCGCGCTCCTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCAc                                                                                                                                              <  1:1263591/150‑1 (MQ=255)
              ggATGCCGGCCGCCAGTGCGCGCTCCTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTtga                                                                                                                                       <  1:1830241/150‑1 (MQ=255)
              ggATGCCGGCCGCCAGTGCGCGCGCCTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCATGGCTTTCCACGTCTtga                                                                                                                                       <  2:488189/150‑1 (MQ=255)
               gATGCCGGCCGCCAGTGCGCGCTCCTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTtgat                                                                                                                                      <  2:1353194/150‑1 (MQ=255)
                 tGCCGGCCGCCAGTGCGCGCTCCTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTtgatga                                                                                                                                    <  1:1837535/150‑1 (MQ=255)
                  gccggccgCCAGTGCGCGCTCCTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAg                                                                                                                                   <  1:543162/150‑1 (MQ=255)
                  gccggccgCCAGTGCGCGCTCCTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAg                                                                                                                                   <  1:39804/150‑1 (MQ=255)
                      gccgccAGTGCGCGCTCCTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTtgat                                                                                                                                      <  1:747345/143‑1 (MQ=255)
                      gccgccAGTGCGCGCTCCTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTtgat                                                                                                                                      >  2:747345/1‑143 (MQ=255)
                                      ccTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGc                                                                                                               >  1:444003/1‑150 (MQ=255)
                                       cTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGcc                                                                                                              >  2:136678/1‑150 (MQ=255)
                                        ttGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCCGGTGTCTGCCt                                                                                                             >  2:610237/1‑150 (MQ=255)
                                        ttGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCt                                                                                                             >  1:1901740/1‑150 (MQ=255)
                                         tGGTGATGATCTTGTCATCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAAGATCAGGTGCCTGCCtt                                                                                                            >  2:975271/1‑150 (MQ=255)
                                             gattagtttGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTc                                                                                                        <  2:679843/143‑1 (MQ=255)
                                             gatgatCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATATTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTc                                                                                                        >  2:1703182/1‑150 (MQ=255)
                                                gatCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCAATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAgg                                                                                                     >  1:1528645/1‑150 (MQ=255)
                                                   cTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGaaa                                                                                                  <  2:1643544/150‑1 (MQ=255)
                                                      gtcgtcGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGcc                                                                                               <  2:828476/150‑1 (MQ=255)
                                                       tcgtcgACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCt                                                                                              <  2:923123/150‑1 (MQ=255)
                                                          tcgACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGACTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGa                                                                                           >  2:86744/1‑150 (MQ=255)
                                                          tcgACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGACTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGa                                                                                           >  2:1028540/1‑150 (MQ=255)
                                                           cgacgaTCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGaa                                                                                          <  2:444003/150‑1 (MQ=255)
                                                               gaTCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGTTGTCTGCCTTGTTCAGGAAAGCCTTGAAgcgc                                                                                      >  1:10190/1‑150 (MQ=255)
                                                                aTCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGACTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCa                                                                                     <  1:86744/150‑1 (MQ=255)
                                                                aTCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGACTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCa                                                                                     <  1:1028540/150‑1 (MQ=255)
                                                                 tCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCAc                                                                                    >  1:1739553/1‑150 (MQ=255)
                                                                   gggTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGa                                                                                  <  2:1700390/150‑1 (MQ=255)
                                                                          ggTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGTTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGt                                                                           >  2:1653050/1‑150 (MQ=255)
                                                                                gcTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCg                                                                     >  2:235050/1‑150 (MQ=255)
                                                                                  ttgttgTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCGCTGAAGTGAAAGAGCTAGGTCGGGGGGt                                                                   >  2:1847536/1‑150 (MQ=255)
                                                                                     ttgtACTTCAGCACATAGTCCGCGTTAACCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGc                                                                >  1:990629/1‑150 (MQ=255)
                                                                                      tgtACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGCGCTCGGTCAGGCGGTAGcc                                                               >  2:442883/1‑150 (MQ=255)
                                                                                        tACTTCAGCACATATTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCcgc                                                             <  1:1703182/150‑1 (MQ=255)
                                                                                        tACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGt                                                                           >  1:1121633/1‑136 (MQ=255)
                                                                                        tACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGt                                                                           <  2:1121633/136‑1 (MQ=255)
                                                                                          cTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAGACATCAGGTGTCTACCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGa                                                           >  2:1036599/1‑150 (MQ=255)
                                                                                           ttCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGAt                                                          >  1:368418/1‑150 (MQ=255)
                                                                                           ttCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGAt                                                          >  1:261275/1‑150 (MQ=255)
                                                                                           ttCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGAGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCCAGGAGGCGGTAGCCGCGAt                                                          >  1:97694/1‑150 (MQ=255)
                                                                                                     aGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCg                                                                             >  1:573051/1‑121 (MQ=255)
                                                                                                     aGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCg                                                                             <  2:573051/121‑1 (MQ=255)
                                                                                                         cgcgTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGcc                                            >  1:1807197/1‑150 (MQ=255)
                                                                                                                  ccGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAg                                   >  1:1741184/1‑150 (MQ=255)
                                                                                                                          tCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAGCCCACCAg                           >  2:1505177/1‑150 (MQ=255)
                                                                                                                          tCCCCATGATGCCCAGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAGCCCACCAg                           >  2:1287338/1‑150 (MQ=255)
                                                                                                                            cccATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGaa                                                                                          <  1:119593/85‑1 (MQ=255)
                                                                                                                            cccATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGaa                                                                                          >  2:119593/1‑85 (MQ=255)
                                                                                                                            cccATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAGCCCACCAGGa                         >  1:1322524/1‑150 (MQ=255)
                                                                                                                                tgatgCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAGCCCACCAGGATcag                     <  1:1653050/150‑1 (MQ=255)
                                                                                                                                 gatgCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGaaa                                                                                                  >  2:693752/1‑72 (MQ=255)
                                                                                                                                 gatgCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGaaa                                                                                                  <  1:693752/72‑1 (MQ=255)
                                                                                                                                 gatgCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGTGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAGCCCACCAGGATcagc                    >  1:327191/1‑150 (MQ=255)
                                                                                                                                 gatcCCCCGGCCCTCCCGAGCTTTACTCGTCACGATAAGGCAACCCTTCTGCTGCCTGCATTGAACAGGACCACCCTGACGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAGCCCACCAGGATaagc                    <  2:863941/150‑1 (MQ=255)
                                                                                                                                  atgCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAGCCCACCAGGATcagca                   <  1:442883/150‑1 (MQ=255)
                                                                                                                                              tCCAGGGCTTTCCACGTCGTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAGCCCACCAGGATCAGCAGCACCGCCGGGa       <  1:1847536/150‑1 (MQ=255)
                                                                                                                                               caaGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAGCCCACCAGGATCAGCAGCACCGCCGGGaa      >  2:1368145/3‑150 (MQ=255)
                                                                                                                                                 aGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAGCCCACCAGGATCAGCAGCACCGCCGGGAAgg    <  1:643041/150‑1 (MQ=255)
                                                                                                                                                   ggCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAGCCCACCAGGATCAGCAGCACCGCCGGGAAGGTg  >  1:755536/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CATTTCCGGCACATGGATGCCGGCCGCCAGTGCGCGCTCCTTGGTGATGATCTTGTCGTCGACGATCGGGTACAGGTTGCGCTTGTTGTACTTCAGCACATAGTCCGCGTTACGCCGGTTGATCCCCATGATGCCCCGGGCCTCCAGGGCTTTCCACGTCTTGATGAGGCCAAACATCAGGTGTCTGCCTTGTTCAGGAAAGCCTTGAAGCGCACGAGCTCGGTCAGGCGGTAGCCGCGATAACGGCCCATCGCCAGCATGAAGCCCACCAGGATCAGCAGCACCGCCGGGAAGGTG  >  NC_002947/2658667‑2658963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑