Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,693,846 G→A 9.1% A32T (GCC→ACC)  PP_2362 → usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,693,8460GA 9.1% 206.6 / 9.8 77A32T (GCC→ACC) PP_2362usher protein
Reads supporting (aligned to +/- strand):  ref base G (31/39);  new base A (5/2);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.14e-01

TGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGCGGGCC  >  NC_002947/2693701‑2693992
                                                                                                                                                 |                                                                                                                                                  
tGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCAt                                                                                                                                                >  1:1190317/1‑150 (MQ=255)
   tCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGt                                                                                                                                             <  1:1014243/150‑1 (MQ=255)
     aaCACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCa                                                                                                                                           >  1:757602/1‑150 (MQ=255)
        acTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGcc                                                                                                                                        >  1:180417/1‑150 (MQ=255)
         cTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGGCGAGTGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCGGGTTCCTGGGGCCGGTCCTGGTCAGgcc                                                                                                                                       <  2:793485/150‑1 (MQ=255)
                gACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAGCCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTg                                                                                                                                <  2:693362/150‑1 (MQ=255)
                gACGCCAGCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGACAGGGAGGACGTGGTAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTg                                                                                                                                <  2:56914/150‑1 (MQ=255)
                   gCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTa                                                                                                                             <  1:779261/150‑1 (MQ=255)
                   gCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTa                                                                                                                             >  1:193063/1‑150 (MQ=255)
                     cATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGCGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACt                                                                                                                           >  1:730751/1‑150 (MQ=255)
                      aTCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTg                                                                                                                          >  2:1631548/1‑150 (MQ=255)
                      aTCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGACCATGGCCAGcccca                                                                                                                                     <  2:376262/139‑2 (MQ=255)
                      aTCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGACCATGGCCAGcccca                                                                                                                                     >  1:376262/1‑138 (MQ=255)
                       tCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTgg                                                                                                                         >  1:676925/1‑150 (MQ=255)
                       tCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTgg                                                                                                                         >  1:876765/1‑150 (MQ=255)
                        ccGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGc                                                                                                                        >  2:534160/1‑150 (MQ=255)
                            cAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCcgac                                                                                                                    >  2:343291/1‑150 (MQ=255)
                                gCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACt                                                                                                                >  2:1443005/1‑150 (MQ=255)
                                gCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACt                                                                                                                <  1:1193530/150‑1 (MQ=255)
                                   aaTGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACtt                                                                                                               <  2:325778/148‑1 (MQ=255)
                                   aaTGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACtt                                                                                                               >  1:325778/1‑148 (MQ=255)
                                    aTGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGcc                                                                                                            <  1:1889971/150‑1 (MQ=255)
                                      gAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGnccc                                                                                                          <  2:1186840/150‑1 (MQ=255)
                                           aaCGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCAACCg                                                                                                     >  1:957627/1‑150 (MQ=255)
                                                 cGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACg                                                                                               >  2:707438/1‑150 (MQ=255)
                                                   aaTGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGga                                                                                             >  2:1765480/1‑150 (MQ=255)
                                                    aTGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGgag                                                                                            <  2:552859/150‑1 (MQ=255)
                                                        aGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGt                                                                                        <  2:569214/150‑1 (MQ=255)
                                                            cAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGc                                                                                    <  2:193063/150‑1 (MQ=255)
                                                                  ccAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGc                                                                              <  1:884414/150‑1 (MQ=255)
                                                                     gggAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTCGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGa                                                                           <  1:707438/150‑1 (MQ=255)
                                                                      ggaggaCCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAg                                                                          >  2:1448365/1‑150 (MQ=255)
                                                                      ggaggaCCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAg                                                                          <  1:1443005/150‑1 (MQ=255)
                                                                      ggaggaCCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAg                                                                          <  2:477745/150‑1 (MQ=255)
                                                                       gaggaCCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAgg                                                                         <  2:949327/150‑1 (MQ=255)
                                                                          gaCCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCa                                                                      >  1:1107971/1‑150 (MQ=255)
                                                                           aCCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCAc                                                                     <  1:1631548/150‑1 (MQ=255)
                                                                            cccGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCAcc                                                                    <  2:876765/150‑1 (MQ=255)
                                                                             ccGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCAccc                                                                   >  1:106034/1‑150 (MQ=255)
                                                                              cGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCACTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCt                                                                  >  2:1714506/1‑150 (MQ=255)
                                                                               ggCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGAACATAGCCTCTACACCCCGACGACTTGCCCCCACCGCCAACGGAGATGTCCACCATCGCCGAGGCCACCCtt                                                                 >  1:512606/1‑150 (MQ=255)
                                                                                     ggTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTc                                                           <  2:499507/150‑1 (MQ=255)
                                                                                     ggTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTc                                                           >  1:1471582/1‑150 (MQ=255)
                                                                                       ttGGAAACCGTGTGTGAAATGGCTCCCGGCGACGAAGTGACATCGAATCATTTTGTTGGCCATGGTCAGCCCCGCTTATGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGa                                                         <  1:1868578/150‑1 (MQ=255)
                                                                                         ggAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCACCGAGGCCACCCTTTACCTCGAcc                                                       <  2:180417/150‑1 (MQ=255)
                                                                                           aaaCCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCtg                                                     >  1:1264909/1‑150 (MQ=255)
                                                                                            aaCCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCtgg                                                    <  2:752403/150‑1 (MQ=255)
                                                                                             aCCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCtggt                                                   >  2:1024887/1‑150 (MQ=255)
                                                                                              ccGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCtgttg                                                  <  1:1448365/150‑1 (MQ=255)
                                                                                                  gtgtgAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCACCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGa                                              <  1:1668765/150‑1 (MQ=255)
                                                                                                     tgAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAAcc                                           <  2:757602/150‑1 (MQ=255)
                                                                                                      gAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCa                                          <  2:1609514/150‑1 (MQ=255)
                                                                                                         aTGGCTCCCAGCAAAGCCGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGAt                                       <  2:901502/150‑1 (MQ=255)
                                                                                                          tGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACGTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATg                                      <  2:788171/150‑1 (MQ=255)
                                                                                                              tCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCa                                  >  1:84788/1‑150 (MQ=255)
                                                                                                                ccGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAgg                                >  1:1014780/1‑150 (MQ=255)
                                                                                                                ccGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAgg                                <  1:1024887/150‑1 (MQ=255)
                                                                                                                 cGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCcgcg                                                                                                                                   <  2:342260/50‑1 (MQ=255)
                                                                                                                 cGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCcgcg                                                                                                                                   >  1:342260/1‑50 (MQ=255)
                                                                                                                 cGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAggg                               <  2:84788/150‑1 (MQ=255)
                                                                                                                    ggacgacGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTg                            <  1:615166/149‑1 (MQ=255)
                                                                                                                      acgacgTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGAc                          <  2:1378549/150‑1 (MQ=255)
                                                                                                                        gacgTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGCGATGTCCGCCATCGCCGAGGCCACCCTTTACATGGACCTGGTGGTGAACCAGAAGCACAGGGTTGACCt                        >  2:150048/1‑150 (MQ=255)
                                                                                                                          cgTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACNTGa                      <  2:1190317/150‑1 (MQ=255)
                                                                                                                              ccGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCACCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATAcc                  <  2:512606/150‑1 (MQ=255)
                                                                                                                                gCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCgg                <  2:1014780/150‑1 (MQ=255)
                                                                                                                                   ggTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTgc             >  1:493297/1‑150 (MQ=255)
                                                                                                                                   ggTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCAACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTgc             <  2:957627/150‑1 (MQ=255)
                                                                                                                                   ccagcgcggggCTGACCATGGCCAGCCCCACGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTgc             >  1:327275/8‑150 (MQ=255)
                                                                                                                                       cgcggggCTGACCATGGCCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTgcagca         >  1:430826/4‑150 (MQ=255)
                                                                                                                                         tGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCagcagc       >  2:1686832/1‑150 (MQ=255)
                                                                                                                                         tGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCACAGGGTTGACCTGATACCGGTGCagcagc       >  1:1241659/1‑150 (MQ=255)
                                                                                                                                         tGGGGCTGGCCATCGTCAGCCTCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCagcagc       <  1:150048/150‑1 (MQ=255)
                                                                                                                                           gggCTGACCATGGCCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAAcc                                           <  2:258085/112‑1 (MQ=255)
                                                                                                                                           gggCTGACCATGGCCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAAcc                                           >  1:258085/1‑112 (MQ=255)
                                                                                                                                            ggCTGACCATGGCCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGcggg    >  2:797952/1‑150 (MQ=255)
                                                                                                                                              cTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGcgggcc  >  1:297653/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TGGTCAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGACTTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGCGGGCC  >  NC_002947/2693701‑2693992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑