BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,777,050	C→G	5.2%	W166C (TGG→TGC)	PP_2429 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,777,050	0	C	G	5.2%	356.3 / 7.2	114	W166C (TGG→TGC)	PP_2429	membrane protein
Reads supporting (aligned to +/- strand): ref base C (60/47); new base G (4/2); total (65/49)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 116 total aligned reads displayed.

GAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTT  >  NC_002947/2776906‑2777199

gAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGNTCAANTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGGTGACCACAAAACCCCCGCCAACACTGGGTTGGAATCATACTACCTCCCCGGTGCCTGCACCCCCC‑g                                                                                                                                                  >  1:641/1‑150 (MQ=255)

 gggCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑gg                                                                                                                                                 <  2:1318236/149‑1 (MQ=255)

 aGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑gg                                                                                                                                                 <  1:192962/150‑1 (MQ=255)

 aGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑gg                                                                                                                                                 <  1:454253/150‑1 (MQ=255)

     gTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGgcgc                                                                                                                                             >  2:1822878/1‑150 (MQ=255)

      ttGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCt                                                                                                                                            >  2:243678/1‑150 (MQ=255)

      ttGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCt                                                                                                                                            >  1:1602575/1‑150 (MQ=255)

        gACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGcccc                                                                                                                                          >  2:33665/1‑150 (MQ=255)

         acacGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTccc                                                                                                                                         <  1:243678/150‑1 (MQ=255)

                  gCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCaa                                                                                                                                >  2:322578/1‑150 (MQ=255)

                        ttcGCTCAGGCCGCTTGTTGGTCAAGTATTTTTTCAGTCCAATACTGAAGTCTATGCCGATATCAACAAAACCCCCGCCAACAGTTGGTTGAATATTCGCTACCTCTCCTTTTCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGc                                                                                                                          >  2:451171/3‑150 (MQ=255)

                          cGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGccc                                                                                                                        >  2:1686543/1‑150 (MQ=255)

                           gatCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGTAGCCCATGCCGAGATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCa                                                                                                                       >  2:1756595/3‑150 (MQ=255)

                                 gCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGGCTGCAGGCACC‑ggg                                                                                                                                                <  1:574278/119‑1 (MQ=255)

                                  ccccTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACCCCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAactc                                                                                                                             <  2:71603/137‑3 (MQ=255)

                                  ccccTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAacac                                                                                                                             >  1:71603/1‑137 (MQ=255)

                                   cccTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCa                                                                                                               <  1:1233222/150‑1 (MQ=255)

                                   cccTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCa                                                                                                               >  2:1831942/1‑150 (MQ=255)

                                    ccTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCAt                                                                                                              >  2:1495817/1‑150 (MQ=255)

                                    ccTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCAt                                                                                                              >  1:114689/1‑150 (MQ=255)

                                            gTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGcc                                                                                                                         <  1:93493/131‑1 (MQ=255)

                                            gTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGcc                                                                                                                         >  2:93493/1‑131 (MQ=255)

                                            gTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCa                                                                                                      <  1:121512/150‑1 (MQ=255)

                                            gTCAAGTAGAGGCGCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCa                                                                                                      <  1:322578/150‑1 (MQ=255)

                                             tCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCag                                                                                                     >  2:1036884/1‑150 (MQ=255)

                                               aaGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCagag                                                                                                   >  1:1050163/1‑150 (MQ=255)

                                               aaGTAGAGGCGCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCAccccc                                                                                                                                  >  2:482478/1‑119 (MQ=255)

                                                 gTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCg                                                                                                 >  2:547349/1‑150 (MQ=255)

                                                 gTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCg                                                                                                 >  1:622509/1‑150 (MQ=255)

                                                 gTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCg                                                                                                 >  1:980744/1‑150 (MQ=255)

                                                      ggCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAg                                                                                            >  1:1794241/1‑150 (MQ=255)

                                                       gCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCa                                                                                             <  2:1155615/148‑1 (MQ=255)

                                                       gCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCa                                                                                             >  1:1155615/1‑148 (MQ=255)

                                                        cTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCAATGCTCGGCAGAGCGATCAGc                                                                                           >  1:1913806/1‑149 (MQ=255)

                                                        cTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCAATGCTCGGCAGAGCGATCAGc                                                                                           <  2:1913806/149‑1 (MQ=255)

                                                         tCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTc                                                                                                                                           >  1:516472/1‑100 (MQ=255)

                                                         tCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTc                                                                                                                                           <  2:516472/100‑1 (MQ=255)

                                                         tCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGCGCGATCCGCaa                                                                                         >  2:856287/1‑150 (MQ=255)

                                                         tCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCaa                                                                                         <  2:1794241/150‑1 (MQ=255)

                                                          cAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAg                                                                                        <  1:1495112/150‑1 (MQ=255)

                                                            ggCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGaa                                                                                      <  1:1036884/150‑1 (MQ=255)

                                                             gCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAac                                                                                     >  2:1530341/1‑150 (MQ=255)

                                                               cAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAacac                                                                                   <  2:114689/150‑1 (MQ=255)

                                                               cAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTAAATGAAGTCACC‑GAGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAacac                                                                                   >  1:1875487/1‑150 (MQ=255)

                                                                   aCTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAgt                                                                               >  1:1897686/1‑149 (MQ=255)

                                                                   aCTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAgt                                                                               >  1:722311/1‑149 (MQ=255)

                                                                     tGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAgcgc                                                                             >  2:1258538/1‑150 (MQ=255)

                                                                          cacATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCt                                                                        >  2:472027/3‑150 (MQ=255)

                                                                           ccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCaa                                                                                         <  1:1639918/132‑1 (MQ=255)

                                                                            cATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGc                                                                      >  2:619604/1‑150 (MQ=255)

                                                                            cATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGc                                                                      <  1:472027/150‑1 (MQ=255)

                                                                              tGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCca                                                                    <  1:1201567/150‑1 (MQ=255)

                                                                                   atatCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTg                                                               <  2:822782/150‑1 (MQ=255)

                                                                                    tatCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTgg                                                              <  1:619604/150‑1 (MQ=255)

                                                                                     atCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGc                                                             >  1:1428600/1‑150 (MQ=255)

                                                                                       caacaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCaa                                                           <  1:1831942/150‑1 (MQ=255)

                                                                                        aacaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCca                                                                    <  1:1433267/140‑1 (MQ=255)

                                                                                        aacaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCca                                                                    >  2:1433267/1‑140 (MQ=255)

                                                                                        aacaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAac                                                          >  2:1179532/1‑150 (MQ=255)

                                                                                        aacaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAac                                                          >  2:171563/1‑150 (MQ=255)

                                                                                         acaaAACCCCCGTCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTCCCACACTGTCAacg                                                         >  2:1384640/1‑150 (MQ=255)

                                                                                         acaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAggcgg                                                                                                                  <  2:1823654/93‑1 (MQ=255)

                                                                                         acaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAggcgg                                                                                                                  >  1:1823654/1‑93 (MQ=255)

                                                                                         acaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAacac                                                                                   >  2:618875/1‑124 (MQ=255)

                                                                                         acaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAacac                                                                                   <  1:618875/124‑1 (MQ=255)

                                                                                            aaaCCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAacgacg                                                      <  2:1875487/150‑1 (MQ=255)

                                                                                             aaCCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGGCCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACgg                                                     >  1:904167/1‑150 (MQ=255)

                                                                                                ccccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGgcgc                                                  <  2:1602575/150‑1 (MQ=255)

                                                                                                 cccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAac                                                          <  2:939467/141‑1 (MQ=255)

                                                                                                 cccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAac                                                          <  2:938631/141‑1 (MQ=255)

                                                                                                 cccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAac                                                          >  1:938631/1‑141 (MQ=255)

                                                                                                 cccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAac                                                          >  1:939467/1‑141 (MQ=255)

                                                                                                 cccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGcgcg                                                 >  2:196404/1‑150 (MQ=255)

                                                                                                       aaCACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAAt                                           <  1:1686543/150‑1 (MQ=255)

                                                                                                                ttGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGc                                                             >  1:238622/1‑123 (MQ=255)

                                                                                                                ttGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGc                                                             <  2:238622/123‑1 (MQ=255)

                                                                                                                  gAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCCAGCACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGaccac                                >  2:222661/1‑150 (MQ=255)

                                                                                                                    acacCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCg                              >  1:217049/1‑150 (MQ=255)

                                                                                                                       cccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGccc                           >  2:367576/1‑149 (MQ=255)

                                                                                                                       cccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCa                           >  2:433915/1‑150 (MQ=255)

                                                                                                                        ccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCAc                          >  1:1387969/1‑150 (MQ=255)

                                                                                                                        ccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCAc                          >  1:950806/1‑150 (MQ=255)

                                                                                                                        ccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCAc                          >  2:1619567/1‑150 (MQ=255)

                                                                                                                        ccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCAc                          >  2:8944/1‑150 (MQ=255)

                                                                                                                                cgcccGGTGGCTGCAGGCACCGGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGgcg                                                   >  1:1777555/3‑118 (MQ=255)

                                                                                                                                cgcccGGTGGCTGCAGGCACCGGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGgcg                                                   <  2:1777555/116‑1 (MQ=255)

                                                                                                                                  cccGGTGGCTGCAGGCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAAc                >  2:1672385/1‑150 (MQ=255)

                                                                                                                                  cccGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAAc                <  2:1387969/150‑1 (MQ=255)

                                                                                                                                   ccGGTGGCTGCAGGCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAAcc               >  1:757071/1‑150 (MQ=255)

                                                                                                                                    cGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCa              <  1:1495817/150‑1 (MQ=255)

                                                                                                                                    cGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCa              <  1:171563/150‑1 (MQ=255)

                                                                                                                                    cGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCCGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCa              <  1:1756595/150‑1 (MQ=255)

                                                                                                                                           tGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTg       >  2:652201/1‑150 (MQ=255)

                                                                                                                                           tGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTg       >  1:21828/1‑150 (MQ=255)

                                                                                                                                            gCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTg                                                               >  1:87845/1‑93 (MQ=255)

                                                                                                                                            gCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTg                                                               <  2:87845/93‑1 (MQ=255)

                                                                                                                                             cAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTggg     <  2:1428600/150‑1 (MQ=255)

                                                                                                                                              aGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGc                                                                                           <  2:678763/63‑1 (MQ=255)

                                                                                                                                              aGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGc                                                                                           >  1:678763/1‑63 (MQ=255)

                                                                                                                                                ccACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCACGCCCGACCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGtt  >  2:267912/1‑150 (MQ=255)

GAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTT  >  NC_002947/2776906‑2777199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`