Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,779,159 T→G 5.5% E81A (GAA→GCA)  PP_2431 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,779,1590TG 5.5% 226.2 / 3.9 82E81A (GAA→GCA) PP_2431LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (38/39);  new base G (2/3);  total (40/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

CTGGTCGGACAGGAAGAAGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTCGCGTTCCAGGCGTTTGATTGCGGCCGCCACCTGG  >  NC_002947/2779011‑2779308
                                                                                                                                                    |                                                                                                                                                     
cTGGTCGGACAGGAAGAAGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTc                                                                                                                                                      <  2:29852/150‑1 (MQ=255)
 tGGTCGGACAGGAAGAAGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCa                                                                                                                                                     >  2:1787643/1‑150 (MQ=255)
 ctaacGGACAGGAAGAAGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGGTGCCATGCAGATCTTCa                                                                                                                                                     >  1:1500321/5‑150 (MQ=255)
   gTCGGACAGGAAGAAGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATg                                                                                                                                                   >  1:1463326/1‑150 (MQ=255)
    tCGGACAGGAAGAAGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATgg                                                                                                                                                  >  1:1885669/1‑150 (MQ=255)
      ggACAGATAGAAGCGCAGATGCAACGCCGGGTGGTGGGGGCGGAAGGGGCTGAGCCAGGGCAGAAGCATGTTGCGGCCCATGTCGGAAGCGGCTGATACCTGCAGGGTGCCGCGCAGGCTAGTGGGGTTGGCATGCAGATCTTCATGGcc                                                                                                                                                <  2:369180/150‑1 (MQ=255)
       gACAGGAAGAAGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGccc                                                                                                                                               >  2:1362294/1‑150 (MQ=255)
        aCAGGAAGAAGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCt                                                                                                                                              <  1:1275764/150‑1 (MQ=255)
         cAGGAAGAAGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTg                                                                                                                                             <  1:1912210/150‑1 (MQ=255)
          aGGAAGAAGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTgg                                                                                                                                            <  2:1885669/150‑1 (MQ=255)
          aGGAAGAAGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTgg                                                                                                                                            >  2:1319680/1‑150 (MQ=255)
          aGGAAGAAGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCCATGGGGGTTGCCATGCAGATCTTCATGGCCCTgg                                                                                                                                            >  1:8794/1‑150 (MQ=255)
            gaagaaGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGCAGGAGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCACGCAGATCTTCATGGCCCTGgcg                                                                                                                                          >  2:786393/1‑150 (MQ=255)
            gaagaaGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGgcg                                                                                                                                          >  2:597785/1‑150 (MQ=255)
            gaagaaGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGgcg                                                                                                                                          >  2:1760573/1‑150 (MQ=255)
               gaagCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGCAGGGGCCGCTACCTGCAGGGTGCCGCGCAGGCTATTCGGGTTGCCATGCAGATATTCATGGCCCTGGCGCAg                                                                                                                                       >  2:524640/1‑150 (MQ=255)
               gaagCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCCGGGTGCTGTGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAg                                                                                                                                       <  1:1787643/150‑1 (MQ=255)
               gaagCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGAAGCAGCAGGTTGCGGCCCATGTCGGAAGGGGCCGATACCTACAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCNCAg                                                                                                                                       <  2:1203738/150‑1 (MQ=255)
                  gcgcAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCt                                                                                                                                    <  1:1319680/150‑1 (MQ=255)
                   cgcAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCtt                                                                                                                                   >  1:346191/1‑150 (MQ=255)
                    gcAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCttt                                                                                                                                  <  1:562172/150‑1 (MQ=255)
                     cAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTc                                                                                                                                 <  1:1760573/150‑1 (MQ=255)
                      aGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGccgaat                                                                                                                                <  2:28609/150‑6 (MQ=255)
                       gTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCa                                                                                                                               <  1:956879/150‑1 (MQ=255)
                           cAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCAGGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCAGGGCCCTGGCGCAGGCTTTCCAGcac                                                                                                                           >  2:670586/1‑150 (MQ=255)
                            aaTGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGcaca                                                                                                                          >  2:1615103/1‑150 (MQ=255)
                              tGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACAtt                                                                                                                        >  1:1427284/1‑150 (MQ=255)
                               gCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTc                                                                                                                       <  1:1362294/150‑1 (MQ=255)
                                    gtggtCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAgcgc                                                                                                                  <  2:1427284/150‑1 (MQ=255)
                                    gtggtCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAgcgc                                                                                                                  >  2:1216111/1‑150 (MQ=255)
                                      ggtCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCgg                                                                                                                <  2:1463326/150‑1 (MQ=255)
                                        tCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGtt                                                                                                              <  1:484798/150‑1 (MQ=255)
                                        tCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGtt                                                                                                              >  2:1667274/1‑150 (MQ=255)
                                         cgcgGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTg                                                                                                             <  1:1188826/150‑1 (MQ=255)
                                            ggcggAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCAATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCa                                                                                                          >  1:212999/1‑150 (MQ=255)
                                                   ggCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAgcg                                                                                                   <  2:750462/150‑1 (MQ=255)
                                                     cgcTGAGCCAGGGCAGCAGCATGTTGCGGCCAATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCt                                                                                                 <  2:212999/150‑1 (MQ=255)
                                                       cTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCg                                                                                               >  2:1674301/1‑150 (MQ=255)
                                                        tGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTTCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGc                                                                                                           >  1:438208/1‑137 (MQ=255)
                                                        tGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGc                                                                                                           <  2:438208/137‑1 (MQ=255)
                                                         gAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGcc                                                                                             <  1:736563/150‑1 (MQ=255)
                                                         gAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGcc                                                                                             <  2:1157963/150‑1 (MQ=255)
                                                            ccAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTc                                                                                          >  2:224560/1‑150 (MQ=255)
                                                            ccAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTc                                                                                          >  2:1558293/1‑150 (MQ=255)
                                                             cAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTcg                                                                                         >  1:1766255/1‑150 (MQ=255)
                                                                  cagcagcaTGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCGTGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGt                                                                                    <  2:1309586/150‑1 (MQ=255)
                                                                     cagcaTGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAg                                                                                 >  1:1567074/1‑150 (MQ=255)
                                                                        caTGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGgcg                                                                              <  1:1891959/150‑1 (MQ=255)
                                                                            ttGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAgg                                                                          >  2:1649907/1‑150 (MQ=255)
                                                                            ttGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAgg                                                                          <  1:275895/150‑1 (MQ=255)
                                                                                 gCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCg                                                                     >  2:716010/1‑150 (MQ=255)
                                                                                   ccATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCggg                                                                   <  2:434319/150‑1 (MQ=255)
                                                                                    cATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCATGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTTGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGggt                                                                  >  1:537109/1‑150 (MQ=255)
                                                                                        tCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGgtggtg                                                              >  1:1852322/1‑150 (MQ=255)
                                                                                        tCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGgtggtg                                                              >  2:1899388/1‑150 (MQ=255)
                                                                                           gAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCgg                                                           <  2:131642/150‑1 (MQ=255)
                                                                                            aaGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCggg                                                          <  1:1272242/150‑1 (MQ=255)
                                                                                              ggggCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCg                                                        >  2:887492/1‑150 (MQ=255)
                                                                                                  ccGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACa                                                    >  1:1001941/1‑150 (MQ=255)
                                                                                                  ccGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACa                                                    <  1:887492/150‑1 (MQ=255)
                                                                                                  ccGATACCTGCAGGGTGCCGCGCAGGCTAGTGGGGTTGGCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAAcc                                                    >  2:216/1‑149 (MQ=255)
                                                                                                     aTACCTGCAGGGTGCCGCGCATGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGc                                                 <  2:537109/150‑1 (MQ=255)
                                                                                                     aTACCTGCAGGGTGCCGCGCAGGCTCTTGGGGGTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACNGGc                                                 <  2:1185930/150‑1 (MQ=255)
                                                                                                            gaGGGTGCTGCGCAGGCTATTGGGGTTGCCATTCAGATCTGCATGGCTCTTGCGCAGGCTTTCCAGCACACTCAGCGCGCTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGGGCGGGCGAACAGGCGTACATc                                          <  2:611256/149‑1 (MQ=255)
                                                                                                            cAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATc                                          <  2:1001941/150‑1 (MQ=255)
                                                                                                                    cgcgCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTcgc                                  >  2:848583/1‑150 (MQ=255)
                                                                                                                           gCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTCGCGTTCCAg                           >  2:658451/1‑150 (MQ=255)
                                                                                                                            cTATTGGGGTTGCCATGCAGATCTTCCTGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAg                                                                                 <  2:1092246/95‑1 (MQ=255)
                                                                                                                            cTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAg                                                                                 >  1:1092246/1‑95 (MQ=255)
                                                                                                                             tATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTCGCGTTCCAGGc                         >  2:259693/1‑150 (MQ=255)
                                                                                                                               ttGGGGTTGCCATGCAGATCGTCATGGCCCTGGCGCAGGCTTTCCAGCAACTTCAGCGCGGTTGGCAGGTAGCGCTCGCGCTCGGCGGTAAGGCGCAGGCGGCGGGTGGGGCGGGCGAAGAGGCGTACATCCAGGTCGCGTTCCAGGCGt                       >  2:193675/1‑150 (MQ=255)
                                                                                                                                tGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTCGCGTTCCAGGCGtt                      <  1:537499/150‑1 (MQ=255)
                                                                                                                                 ggggTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTCGCGTTCCAGGCGttt                     <  2:346191/150‑1 (MQ=255)
                                                                                                                                   ggTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTAGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTCGCGTTCCAGGCGTTTGa                   <  1:1674301/150‑1 (MQ=255)
                                                                                                                                    gTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTCGCGTTCCAGGCGTTTGAt                  <  2:8794/150‑1 (MQ=255)
                                                                                                                                     ttGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTCGCGTTCCAGGCGTTTGAtt                 <  1:749918/150‑1 (MQ=255)
                                                                                                                                       gCCATGAAGATCTGCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCggg                                                                   <  1:719869/98‑1 (MQ=255)
                                                                                                                                       gCCATGAAGATCTGCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCggg                                                                   >  1:1034653/1‑98 (MQ=255)
                                                                                                                                       gCCATGAAGATCTGCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCggg                                                                   <  2:1034653/98‑1 (MQ=255)
                                                                                                                                       gCCATGAAGATCTGCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCggg                                                                   >  2:719869/1‑98 (MQ=255)
                                                                                                                                           tGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTCGCGTTCCAGGCGTTTGATTGCGgcc           >  2:1208029/1‑150 (MQ=255)
                                                                                                                                            gCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTCGCGTTCCAGGCGTTTGATTGCGgccg          <  1:259693/150‑1 (MQ=255)
                                                                                                                                                   ttCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTCGCGTTCCAGGCGTTTGATTGCGGCCGCCACCTg   >  1:1566700/1‑150 (MQ=255)
                                                                                                                                                   ttCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTCGCGTTCCAGGCGTTTGATTGCGGCCGCCACCTg   <  1:786393/150‑1 (MQ=255)
                                                                                                                                                    tCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCCGACGCGTTCTGCGGGTGGTGCTTCCTAACAGGCGTACATCCAGGTCGCGTTCCAGGCGTTTGATTGCGGCCGCCACCTgg  <  1:1177480/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGGTCGGACAGGAAGAAGCGCAGTTGCAATGCCGGGTGGTCGCGGCGGAAGGCGCTGAGCCAGGGCAGCAGCATGTTGCGGCCCATGTCGGAAGGGGCCGATACCTGCAGGGTGCCGCGCAGGCTATTGGGGTTGCCATGCAGATCTTCATGGCCCTGGCGCAGGCTTTCCAGCACATTCAGCGCGGTTGGCAGGTAGCGCTCGCCCTCGGCGGTCAGGCGCAGGCTGCGGGTGGTGCGGGCGAACAGGCGTACATCCAGGTCGCGTTCCAGGCGTTTGATTGCGGCCGCCACCTGG  >  NC_002947/2779011‑2779308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑