Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,812,824	A→G	9.8%	intergenic (+75/‑35)	rplT → / → pheS	50S ribosomal protein L20/phenylalanine‑‑tRNA ligase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,812,824	0	A	G	9.8%	163.6 / 7.4	61	intergenic (+75/‑35)	rplT/pheS	50S ribosomal protein L20/phenylalanine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (28/27);  new base G (3/3);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

GGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGGCGAGCT  >  NC_002947/2812677‑2812971                                                                                                                                                    |                                                                                                                                                     ggCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGc                                                                                                                                                    >  2:634634/1‑150 (MQ=255)     gATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGACTTCCTTGTTCGCTACAATCTCTCGGCGGTGCTAGCGCGGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCtt                                                                                                                                                <  1:118454/150‑1 (MQ=255)        cTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTTGAAGGCTCTTccc                                                                                                                                             <  2:1238574/150‑1 (MQ=255)          ggCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCt                                                                                                                                           >  2:1793768/1‑150 (MQ=255)                    gAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTAt                                                                                                                                 <  2:803953/150‑1 (MQ=255)                      aaaaGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCt                                                                                                                               >  1:252977/1‑150 (MQ=255)                          gcggcgTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAg                                                                                                                           >  1:617044/1‑150 (MQ=255)                           cggcgTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGNAGt                                                                                                                          <  2:1187019/150‑1 (MQ=255)                                tttGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTa                                                                                                                     <  2:617044/150‑1 (MQ=255)                                     tgcGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGa                                                                                                                            <  1:1137236/138‑1 (MQ=255)                                     tgcGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGa                                                                                                                            >  2:1137236/1‑138 (MQ=255)                                        gATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGACAGTCATCGATAGGGGAAGAGCCTTCACAGCTCGTCCCCAATTTTCGTGTCTGGAAGCTGCACATGGcaa                                                                                                             >  1:205270/1‑150 (MQ=255)                                               gagaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGa                                                                                                     >  2:854638/1‑150 (MQ=255)                                               gagaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGa                                                                                                     >  1:333132/1‑150 (MQ=255)                                                    aGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATgcgc                                                                                                >  1:884529/1‑150 (MQ=255)                                                        aaaGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGt                                                                                            <  2:1079817/150‑1 (MQ=255)                                                          aGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGtct                                                                                          >  1:1808714/1‑150 (MQ=255)                                                            ccGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTTAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTcc                                                                                        <  2:919651/150‑1 (MQ=255)                                                             cGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTccc                                                                                       <  1:854638/150‑1 (MQ=255)                                                                    gCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCt                                                                                >  2:1508427/1‑150 (MQ=255)                                                                       tAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTaga                                                                             <  2:1767596/150‑1 (MQ=255)                                                                        aaGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTagag                                                                            <  2:1294993/150‑1 (MQ=255)                                                                          gTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGc                                                                          <  1:634634/150‑1 (MQ=255)                                                                             cccGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGt                                                                       <  1:1793768/150‑1 (MQ=255)                                                                              ccGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTcccc                                                                                                                                            >  2:969716/1‑81 (MQ=255)                                                                              ccGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTcccc                                                                                                                                            <  1:969716/81‑1 (MQ=255)                                                                                gcgACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGa                                                                    >  1:1248263/1‑150 (MQ=255)                                                                                     aaTCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAAcgcg                                                               >  2:599187/1‑150 (MQ=255)                                                                                     aaTCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAAcgcg                                                               >  2:1377628/1‑150 (MQ=255)                                                                                         aCCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTCCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCCAGGTGGCGCATG‑GTGCTAATGCCTAGAGGCCGTGGAACGCGCTGa                                                           >  1:1866924/1‑150 (MQ=255)                                                                                          cccGGCGGTGCTCGCGCTGCCGGGCATTGAACGTCATCGATAGGGTAAAAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCGAAGCCCTAGAGGCCGTGGAACGCGCTGaa                                                          <  1:108456/150‑1 (MQ=255)                                                                                          cccGGCAGCGCTAGCACCGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGaa                                                          >  1:1574206/1‑150 (MQ=255)                                                                                          cccGGCAGCGCTAGCACCGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGaa                                                          >  1:1574040/1‑150 (MQ=255)                                                                                          cccGGCAGCGCTAGCACCGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGaa                                                          <  2:1574040/150‑1 (MQ=255)                                                                                          cccGGCAGCGCTAGCACCGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGaa                                                          <  2:1574206/150‑1 (MQ=255)                                                                                                      aGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGcc                                              <  1:1377628/150‑1 (MQ=255)                                                                                                       gcgcTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGccc                                             <  1:642893/150‑1 (MQ=255)                                                                                                            gCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACCTGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGaa                                        <  2:1808714/150‑1 (MQ=255)                                                                                                              cGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACa                                      >  2:1209495/1‑150 (MQ=255)                                                                                                              cGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACa                                      <  2:333132/150‑1 (MQ=255)                                                                                                                   aTTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTTTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAAAGCGCTGAAGACATCAACGCCCTGGAACAGAtac                                 >  1:418947/1‑148 (MQ=255)                                                                                                                     tGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCgg                               <  2:1248263/150‑1 (MQ=255)                                                                                                                      gAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCggg                              <  1:599187/150‑1 (MQ=255)                                                                                                                      gAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCAACTTTTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCggg                              <  1:1260682/150‑1 (MQ=255)                                                                                                                           tCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAAt                         >  2:315526/1‑150 (MQ=255)                                                                                                                           tCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAAt                         >  1:686262/1‑150 (MQ=255)                                                                                                                            cATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAAtt                        <  2:252977/150‑1 (MQ=255)                                                                                                                             aTCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTa                       >  1:1850218/1‑150 (MQ=255)                                                                                                                              aaaaTAGGGGAAGAGCTTTGAAGGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGaa                                                          <  2:1396454/111‑1 (MQ=38)                                                                                                                              aaaaTAGGGGAAGAGCTTTGAAGGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGaa                                                          >  1:1396454/4‑114 (MQ=38)                                                                                                                                 aTAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTAtctc                   <  1:315526/150‑1 (MQ=255)                                                                                                                                     gggAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTg                                                                                              <  2:1652791/71‑1 (MQ=255)                                                                                                                                     gggAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTg                                                                                              >  1:1652791/1‑71 (MQ=255)                                                                                                                                      ggAAGAGCTTTGAAGGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCg                  <  1:761078/146‑1 (MQ=255)                                                                                                                                      ggAAGAGCTTTGAAGGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCg                  >  2:761078/1‑146 (MQ=255)                                                                                                                                      ggAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCaa              >  2:1904803/1‑150 (MQ=255)                                                                                                                                       gAAGAGCTTTGAAGGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCaag             >  2:1566521/1‑150 (MQ=255)                                                                                                                                           agCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTACTTATCTCGGCAAGAAgg         >  1:760676/1‑150 (MQ=255)                                                                                                                                              cTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGGCg      <  1:1511386/150‑1 (MQ=255)                                                                                                                                               ttCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGGCGa     >  2:540667/1‑150 (MQ=255)                                                                                                                                               ttCAAAGCTCTTCCCCTATTTTCGTATATGGAGGCTGTACATGGGAAG‑CCTGGATGCGCTGGGTTCCCAGGCCCTAGAGGCCGTGGAACGAGCACAAGACAACAACGCCCTGGAATACATCCGTGTTAATCATCTCGGCATGAAGGGCGa     >  2:592296/1‑150 (MQ=255)                                                                                                                                                  aaaGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTAAGAACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGGCGAGCt  >  1:20246/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAA‑CCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTGGAACAGATCCGGGTTAATTATCTCGGCAAGAAGGGCGAGCT  >  NC_002947/2812677‑2812971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑