Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,135,628 G→T 5.8% E80* (GAG→TAG)  PP_2752 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,135,6280GT 5.8% 217.6 / 2.6 70E80* (GAG→TAG) PP_2752hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (40/25);  new base T (2/2);  total (42/28)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

ATGAACAATTCTTCCCGCTAGCCACCTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAAGGCCGAGGCCGACCGGATTCCGCTG  >  NC_002947/3135479‑3135777
                                                                                                                                                     |                                                                                                                                                     
aTGAACCATTCTTCCCGCGAGCCACCTGCCGAGGCGGTGCCTATGACTCCAGCGGCATTCCGGTGTGGGCCGGGATGACCGATTACTGACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAAtgtg                                                                                                                                                       <  2:159273/150‑1 (MQ=25)
aTGAACAATTCTTCCCGCTAGCCACCTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAAtgtg                                                                                                                                                       <  2:1696287/150‑1 (MQ=255)
 tGAACAATTCTTCCCGCTAGCCACCTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTga                                                                                                                                                      <  2:421443/150‑1 (MQ=255)
 tGAACAATTCTTCCCGCTAGCCACCTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTga                                                                                                                                                      <  2:1006492/150‑1 (MQ=255)
 tGAACAATTCTTCCCGATAGCCACCTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTga                                                                                                                                                      <  2:974992/150‑1 (MQ=255)
   aaCAATTCTTCCCGCTAGCCACCTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTgaga                                                                                                                                                    <  1:814484/150‑1 (MQ=255)
      aaTTCTTCCCGCTAGCCACCTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCg                                                                                                                                                 >  1:1806619/1‑150 (MQ=255)
               cGCTAGCCACCTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACgg                                                                                                                                        >  2:284314/1‑150 (MQ=255)
                     ccACCTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGACGCATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCgaga                                                                                                                                  >  1:426492/1‑150 (MQ=255)
                      cACCTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGTGTCAAGCTGGTCTGGCAGGAATGTGCGACCGAGTGGACGGCCGAGaa                                                                                                                                 <  2:1861161/150‑1 (MQ=255)
                       aCCTACCGAGTCGGTGCCTATGCCTGCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAg                                                                                                                                >  1:458120/1‑150 (MQ=255)
                       aCCTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAg                                                                                                                                >  1:1473645/1‑150 (MQ=255)
                        ccTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAgg                                                                                                                               <  1:1535917/150‑1 (MQ=255)
                        ccTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAgg                                                                                                                               <  2:1473645/150‑1 (MQ=255)
                        ccTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAgg                                                                                                                               <  1:284314/150‑1 (MQ=255)
                          tACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGt                                                                                                                                              >  2:911005/1‑133 (MQ=255)
                          tACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGt                                                                                                                                              <  1:911005/133‑1 (MQ=255)
                              gAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCg                                                                                                                         >  1:141474/1‑150 (MQ=255)
                              gAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCg                                                                                                                         >  1:1818262/1‑150 (MQ=255)
                                    gtgcctgtgcctAAAACGGCATTGCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCt                                                                                                                   >  2:557117/1‑150 (MQ=37)
                                    gTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCt                                                                                                                   >  2:343062/1‑150 (MQ=255)
                                         tATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGACTGTGAGACCGCGTGGACGGCCGAGAACGGCATCCCGTGCTACGaa                                                                                                              >  2:445131/1‑149 (MQ=37)
                                         tATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACAGAGTGCACGGCCGAGAAGGGCATCGATCGCTACGAg                                                                                                              >  2:744172/1‑150 (MQ=255)
                                          aTGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAgc                                                                                                             >  1:1509523/1‑150 (MQ=255)
                                              cTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTTAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAg                                                                                                              >  1:1235845/1‑145 (MQ=255)
                                              cTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTTAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAg                                                                                                              <  2:1238942/145‑1 (MQ=255)
                                              cTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTTAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAg                                                                                                              <  2:1235845/145‑1 (MQ=255)
                                              cTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTTAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAg                                                                                                              >  1:1238942/1‑145 (MQ=255)
                                               tCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGGATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTc                                                                                                        >  1:1878288/1‑150 (MQ=255)
                                                     ggCATTCCGGCGTGGGCCGGGATGATCGATTACTTACGCGACATCAACGAAGTCGAAGCGGGAAGCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAAc                                                                                                  <  2:536429/150‑1 (MQ=38)
                                                         ttCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGcc                                                                                              >  1:858538/1‑150 (MQ=255)
                                                          tCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCt                                                                                             <  2:858739/150‑1 (MQ=255)
                                                          tCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCt                                                                                             <  1:1354706/150‑1 (MQ=255)
                                                             ggTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCaa                                                                                          <  2:1509523/150‑1 (MQ=255)
                                                              gtgtGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAAt                                                                                         >  2:106196/1‑150 (MQ=255)
                                                               tgtgGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATg                                                                                        <  1:1640193/150‑1 (MQ=255)
                                                                    gCCNGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCt                                                                                   >  2:1202161/1‑150 (MQ=255)
                                                                         gatgatCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGt                                                                              >  2:1381138/1‑150 (MQ=255)
                                                                         gatgatCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGt                                                                              >  1:769556/1‑150 (MQ=255)
                                                                           tgatCGATTACTTACGCTACATCAACGAAGTCGAAGGAGGCATACATGACGCCCAGCAGATCTCCCCGCCAGGTGCCACCGCGTGGACTTCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTgg                                                                            <  2:214591/150‑1 (MQ=255)
                                                                              tCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCATGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCgg                                                                         >  1:136143/1‑150 (MQ=255)
                                                                               cGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGt                                                                        <  2:1806619/150‑1 (MQ=255)
                                                                                gATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTc                                                                       >  1:11629/1‑150 (MQ=255)
                                                                                gATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTc                                                                       >  2:425707/1‑150 (MQ=255)
                                                                                     cttacGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAgg                                                                                                                               <  1:1120026/89‑1 (MQ=255)
                                                                                     cttacGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAgg                                                                                                                               >  2:1120026/1‑89 (MQ=255)
                                                                                      ttacGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAg                                                                 >  1:670476/1‑150 (MQ=255)
                                                                                           cTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTAc                                                                    >  1:1234946/1‑142 (MQ=255)
                                                                                           cTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTAc                                                                    <  2:1234946/142‑1 (MQ=255)
                                                                                            tccATCAACGAATTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTCAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAAt                                                           <  2:136143/148‑1 (MQ=255)
                                                                                               aTCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGACGTGCTCGGATTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGt                                                        >  1:1059242/1‑150 (MQ=38)
                                                                                               aTCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGt                                                        <  1:343062/150‑1 (MQ=255)
                                                                                                tCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTg                                                       >  2:450048/1‑150 (MQ=255)
                                                                                                      aaGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCgg                                                 <  1:106196/150‑1 (MQ=255)
                                                                                                       aGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGc                                                <  1:450048/150‑1 (MQ=255)
                                                                                                         tCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGGGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTg                                              >  2:1468539/1‑150 (MQ=255)
                                                                                                           gAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGcc                                            <  2:717662/150‑1 (MQ=255)
                                                                                                                  gcATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGccc                                     >  2:1184096/1‑150 (MQ=255)
                                                                                                                       aaTGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCg                                <  1:1381138/150‑1 (MQ=255)
                                                                                                                        aTGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCCTCAAGAACGGCCGCAATGGCGCTCCGGGGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCCATGCCCTGGCgg                               >  2:121963/1‑150 (MQ=255)
                                                                                                                              tCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAagg                         >  2:252070/1‑150 (MQ=255)
                                                                                                                                aaGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAaggcc                       <  1:1184096/150‑1 (MQ=255)
                                                                                                                                  gCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAaggccga                     >  1:1582899/1‑150 (MQ=255)
                                                                                                                                  gCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAaggccga                     >  2:1750659/1‑150 (MQ=255)
                                                                                                                                   cTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGGATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCACGTGGCGGTCTACCATCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAaggccgag                    >  1:881600/1‑150 (MQ=255)
                                                                                                                                   cTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAaggccgag                    <  2:1766111/150‑1 (MQ=255)
                                                                                                                                     gggcTGGCAGGAATGTGAGAGCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCGATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAaggccgaggc                  <  2:728952/147‑1 (MQ=255)
                                                                                                                                      gTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAaggccgaggcc                 >  2:1751497/1‑150 (MQ=255)
                                                                                                                                          ggCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAAGGCCGAGGCCGAcc             <  1:772331/150‑1 (MQ=255)
                                                                                                                                           gCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCg                                                  >  1:919239/1‑112 (MQ=255)
                                                                                                                                           gCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCg                                                  <  2:919239/112‑1 (MQ=255)
                                                                                                                                           gCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCt                                               >  1:1851720/1‑115 (MQ=255)
                                                                                                                                           gCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCt                                               <  2:1851720/115‑1 (MQ=255)
                                                                                                                                           gCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAAGGCCGAGGCCGACCg            >  1:130386/1‑150 (MQ=255)
                                                                                                                                           gCAGGAATGTGAGACCGAGTGGACGGCCGAGAAAGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAAGGCCGAGGCCGACCg            >  1:1569623/1‑150 (MQ=255)
                                                                                                                                             aGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAAGGCCGAGGCCGACCGGa          >  1:540822/1‑150 (MQ=255)
                                                                                                                                                   gtgAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAAGGCCGAGGCCGACCGGATTcctc    <  1:1751497/150‑3 (MQ=255)
                                                                                                                                                    tgAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAAGGCCGAGGCCGACCGGATTCCGCt   <  1:1905533/150‑1 (MQ=255)
                                                                                                                                                     gagaCCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTGTGCCCTGGCGGACAAGGCCGAGGCCGACCGGATTCCGCTg  >  2:1174767/1‑150 (MQ=255)
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ATGAACAATTCTTCCCGCTAGCCACCTACCGAGTCGGTGCCTATGCCTCCAGCGGCATTCCGGTGTGGGCCGGGATGATCGATTACTTACGCTACATCAACGAAGTCGAAGGCGGCATCAATGGCGTCAAGCTGGTCTGGCAGGAATGTGAGACCGAGTGGACGGCCGAGAAGGGCATCGAGTGCTACGAGCGCTTCAAGAACGGCCTCAATGGCGCTCCGGTGGCGGTCTACCAGCCCAATGGTGCGCCGGCTGCCTATGCCCTGGCGGACAAGGCCGAGGCCGACCGGATTCCGCTG  >  NC_002947/3135479‑3135777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑