Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,175,593	T→C	6.3%	I284T (ATC→ACC)	PP_2787 →	transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,175,593	0	T	C	6.3%	181.3 / 2.0	63	I284T (ATC→ACC)	PP_2787	transporter
Reads supporting (aligned to +/- strand):  ref base T (23/36);  new base C (2/2);  total (25/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01

ACCAAACAGGACGACGCTCAGGCCCAGTTGCGTGCCTATCACCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCTGTTCTGCACCATCTGGACCCTTGGCCTGATGGCGCTGC  >  NC_002947/3175445‑3175742                                                                                                                                                     |                                                                                                                                                       aCCAAACAGGACGACGCTCAGGCCCAGTTGCGTGCCTATCACCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAatcatc                                                                                                                                                       >  1:457989/1‑150 (MQ=255) aCCAAACAGGACGACGCTCAGGCCCAGTTGCGTGCCTATCACCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAatcatc                                                                                                                                                       <  2:457989/150‑1 (MQ=255) aCCAAACAGGACGACGCTCAGGCCCAGTTGCGTGCCTATCACCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAatcatc                                                                                                                                                       >  2:1748276/1‑150 (MQ=255)                   cAGGCCCAGTTGCGTGCCTATCACCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATg                                                                                                                                     <  2:1139480/150‑1 (MQ=255)                   cAGGCCCAGTTGCGTGCCTATCACCAGGTCCGCGAGATCATCGCGAAGCACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATg                                                                                                                                     <  2:578328/150‑1 (MQ=255)                     ggCCCAGTTGCGTGCCTATCACCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCt                                                                                                                                   <  1:1128895/150‑1 (MQ=255)                      gCCCAGTTGCGTGCCTATCACCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTg                                                                                                                                  >  2:1090144/1‑150 (MQ=255)                          aGTTGCGTGCCTATCACCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCTCCCACCCGGAGTGCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGTCGATGCTGTTcc                                                                                                                              >  2:1054698/1‑150 (MQ=255)                          aGTTGCGTGCCTATCACCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTcc                                                                                                                              >  1:249648/1‑150 (MQ=255)                          aGTTGCGTGCCTATCACCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTcc                                                                                                                              <  2:13254/150‑1 (MQ=255)                               cGTGCCTATCACCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATc                                                                                                                         <  1:305505/150‑1 (MQ=255)                                      aTCACCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTcc                                                                                                                 <  1:1090144/150‑1 (MQ=255)                                         aCCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCAc                                                                                                                                                 <  2:224851/116‑1 (MQ=255)                                         aCCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCAGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCAc                                                                                                                                                 >  1:224851/1‑116 (MQ=255)                                          ccAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGtt                                                                                                             <  2:752875/150‑1 (MQ=255)                                           cAGGTCCGCGAGATCACCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTg                                                                                                            <  2:262349/150‑1 (MQ=255)                                             ggTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGt                                                                                                          <  1:1896285/150‑1 (MQ=255)                                              gTCCGCGAGATCATCGCGAAGTACCAGGCCGGCCACCCGGAGGTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCGTTGCGGCCGAGCAGGAAGGCATCGATCACGTCCTGGCGGTGCTGTTCGCGATCGTGGTTCttgttgtg                                                                                                          >  2:395233/1‑148 (MQ=255)                                               tCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGCTGTTCATCGCCGGTATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGa                                                                                                        <  2:1032497/150‑1 (MQ=255)                                                  gcgAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACct                                                                                                     <  2:1335257/150‑1 (MQ=255)                                                  gcgAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACct                                                                                                     >  2:389183/1‑150 (MQ=255)                                                       atcatcGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGAc                                                                                                                                              <  1:1739972/105‑1 (MQ=255)                                                       atcatcGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGAc                                                                                                                                              >  2:1739972/1‑105 (MQ=255)                                                            cgcgAAGTACCAGGCCGCCCACCCGGAGTTCACCCCGTATCTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGt                                                                                           <  1:1040521/150‑1 (MQ=255)                                                                   tACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCACCGATCa                                                                                                                                                  >  2:1405240/1‑89 (MQ=255)                                                                   tACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCACCGATCa                                                                                                                                                  >  2:1405495/1‑89 (MQ=255)                                                                   tACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCACCGATCa                                                                                                                                                  <  1:1405495/89‑1 (MQ=255)                                                                   tACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCACCGATCa                                                                                                                                                  <  1:1405240/89‑1 (MQ=255)                                                                      cAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCttt                                                                                 >  2:114828/1‑150 (MQ=255)                                                                      cAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCttt                                                                                 <  1:549027/150‑1 (MQ=255)                                                                      cAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGATGTTTTTCTTCttt                                                                                 >  2:1080209/1‑150 (MQ=255)                                                                        ggCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGCCCTCTCTGCTGGTTTTCTTCTTTct                                                                               >  2:1885394/1‑149 (MQ=255)                                                                                aCCCGGAGTGCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCAc                                                                       >  1:1547465/1‑150 (MQ=255)                                                                                 cccGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACt                                                                      >  2:455588/1‑150 (MQ=255)                                                                                 cccGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACt                                                                      >  1:867476/1‑150 (MQ=255)                                                                                  ccGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTcc                                                                                                                              >  1:727691/1‑94 (MQ=255)                                                                                  ccGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTcc                                                                                                                              <  2:727691/94‑1 (MQ=255)                                                                                          cACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCgtggt                                                             <  1:389183/150‑1 (MQ=255)                                                                                           aCCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCgtggtg                                                            <  1:841921/150‑1 (MQ=255)                                                                                             cGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCgtg                                                               >  2:504370/1‑145 (MQ=255)                                                                                             cGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCgtg                                                               <  1:504370/145‑1 (MQ=255)                                                                                                tGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGAt                                                                                                                                      <  2:625939/72‑1 (MQ=255)                                                                                                tGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGAt                                                                                                                                      >  1:625939/1‑72 (MQ=255)                                                                                                tGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTACTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGcc                                                       <  1:1054698/150‑1 (MQ=255)                                                                                                  aGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGc                                                     >  1:1705114/1‑150 (MQ=255)                                                                                                   ggTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCt                                                    <  1:1306887/150‑1 (MQ=255)                                                                                                    gtgtTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTg                                                   <  1:1748276/150‑1 (MQ=255)                                                                                                        tCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCa                                               <  2:1378984/150‑1 (MQ=255)                                                                                                           tCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAata                                            <  1:1689301/150‑1 (MQ=255)                                                                                                              ccGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATcc                                         <  2:249648/150‑1 (MQ=255)                                                                                                               cGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCt                                        >  2:1635921/1‑150 (MQ=255)                                                                                                                  cATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCTGtt                                     <  1:1902851/150‑1 (MQ=255)                                                                                                                  cATGCCGATCTTCATTCGGGCCCAACAGGAAATCATCGATCATGACCTGGCGATGCTGTGCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCGGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCTGtt                                     <  1:292602/150‑1 (MQ=255)                                                                                                                    tGCCGATCTTCATTGCGGCCCAGGAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTCTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCCCTTGGCGTGGTACTGCCGCTGTTCAATAGCCTGTTCt                                   >  2:946177/1‑150 (MQ=255)                                                                                                                    tGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCTGTTCt                                   <  1:822613/150‑1 (MQ=255)                                                                                                                      ccGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTttcttc                                                                                    <  2:1017320/99‑1 (MQ=255)                                                                                                                      ccGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTttcttc                                                                                    >  1:1017320/1‑99 (MQ=255)                                                                                                                       cGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTTGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTATCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCTGTTCTGCa                                <  2:1095719/150‑1 (MQ=255)                                                                                                                        gATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCTGt                                      >  1:1054105/1‑143 (MQ=255)                                                                                                                        gATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCTGt                                      <  2:1054105/143‑1 (MQ=255)                                                                                                                               aTTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATCTCCTGTTCTGCACCATCTgg                        >  1:143404/1‑150 (MQ=255)                                                                                                                                     gCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCTGTTCTGCACCATCTGGACCCtt                  <  2:547482/150‑1 (MQ=255)                                                                                                                                      cccAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCTGTTCTGCACCATCTGGACCCTTg                 <  1:220047/150‑1 (MQ=255)                                                                                                                                      cccAACAGGAAATCATCGATCACTCCGTGGCGATGCTCTTCCCGATCGT‑GTTTCTGTTCGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCTCTTGGCGTGCCGCTCCCGCTGCTCAATATCCTGTTCTGCACCATCTGGACCCTTg                 <  2:53456/150‑1 (MQ=255)                                                                                                                                            agGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCTGTTCTGCACCATCTGGACCCTTGGCCTGa           >  2:619593/1‑150 (MQ=255)                                                                                                                                             ggAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTCCGGCCGCTGTTCAATATCCTGTTCTGCACCATCTGGACCCTTGGCCTGAt          <  1:987007/150‑1 (MQ=255)                                                                                                                                                    atcGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCTGTTCTGCACCATCTGGACCCTTGGCCTGATGGCgctg   >  2:420111/1‑150 (MQ=255)                                                                                                                                                    atcGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTATTCTTTCGCAAACCAGTTTGCGGGGTGCTGCCGCTGTTCAATATCCGTTTCTGCACCATCTGGACCCTTGTTCTGATGGCgctg   >  1:390658/1‑150 (MQ=255)                                                                                                                                                     tcGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCTGTTCTGCACCATCTGGACCCTTGGCCTGATGGCgctgc  >  2:798468/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                       ACCAAACAGGACGACGCTCAGGCCCAGTTGCGTGCCTATCACCAGGTCCGCGAGATCATCGCGAAGTACCAGGCCGCCCACCCGGAGTTCACCGATGAGGTGTTCATCGCCGGCATGCCGATCTTCATTGCGGCCCAGCAGGAAATCATCGATCACGACCTGGCGATGCTGTTCCCGATCGT‑GTTCCTGTTGGTGACCTCTCTGCTGGTTTTCTTCTTTCGCAAACCACTTGGCGTGGTGCTGCCGCTGTTCAATATCCTGTTCTGCACCATCTGGACCCTTGGCCTGATGGCGCTGC  >  NC_002947/3175445‑3175742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑