Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,252,535	G→A	7.8%	intergenic (+22/+221)	ureG → / ← PP_2851	urease accessory protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,252,535	0	G	A	7.8%	177.8 / 5.3	64	intergenic (+22/+221)	ureG/PP_2851	urease accessory protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/27);  new base A (3/2);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

GGAGCGCGACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTA  >  NC_002947/3252387‑3252684                                                                                                                                                     |                                                                                                                                                      ggAGCGCGACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGc                                                                                                                                                      >  1:439810/1‑150 (MQ=255) ggAGCGCGACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGc                                                                                                                                                      <  1:1277002/150‑1 (MQ=255)  gAGCGCGACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCa                                                                                                                                                     >  2:1599666/1‑150 (MQ=255)  gAGCGCGACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGACTGACAGATCGAGGACTCTGTGCGGGCa                                                                                                                                                     >  2:1679424/1‑150 (MQ=255)        gACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCGACCTGAAGAAGGGCGAGGGGTTGCAGTCCGTGAGGGATGTGATCCACCAGCGCGGGCTGCTGGGGGGGCGAGGATGACAGATCGAGGACTCTGTGCGGGCATGTccc                                                                                                                                               >  2:731135/1‑149 (MQ=255)        gACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCg                                                                                                                                               >  2:1661676/1‑150 (MQ=255)            cccAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGcaca                                                                                                                                           <  1:534128/150‑1 (MQ=255)            cccAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGcaca                                                                                                                                           <  1:670062/150‑1 (MQ=255)              cAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACaga                                                                                                                                         >  2:1683762/1‑150 (MQ=255)              cAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGACATGCCCGCACaga                                                                                                                                         <  1:435921/150‑1 (MQ=255)                 ggtccagggCCGCTGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGAc                                                                                                                                      >  2:58241/8‑150 (MQ=255)                 cgcATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGAc                                                                                                                                      <  1:1599666/150‑1 (MQ=255)                              tggggCCCTGGACCTTCAGCAGCGTGGAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGAGCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCCACGCGGtggct                                                                                                                         <  2:705977/147‑1 (MQ=255)                              aGCGGCCCTGAGCCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGtggct                                                                                                                         <  1:762699/150‑1 (MQ=255)                                    ccTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCg                                                                                                                                               <  2:772864/122‑1 (MQ=255)                                    ccTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCg                                                                                                                                               >  1:772864/1‑122 (MQ=255)                                      tGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCgg                                                                                                                 >  2:364593/1‑150 (MQ=255)                                          ccTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGAATAGATGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGctct                                                                                                             >  1:116056/1‑150 (MQ=255)                                           cTTCAGCAACCTGAAGCAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTg                                                                                                            <  1:176292/150‑1 (MQ=255)                                           cTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTg                                                                                                            >  2:1362548/1‑150 (MQ=255)                                            ttCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGc                                                                                                           <  1:600248/150‑1 (MQ=255)                                            ttCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGc                                                                                                           >  1:758681/1‑150 (MQ=255)                                              cAGCAACCCGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCg                                                                                                         <  2:1044149/150‑1 (MQ=255)                                               aGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCgg                                                                                                        >  2:824672/1‑150 (MQ=255)                                                   aCCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGtt                                                                                                    >  2:141759/1‑150 (MQ=255)                                                        aagaagGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCgg                                                                                               <  1:938375/150‑1 (MQ=255)                                                        aagaagGGCGAGGGGTTGCACGCTCTGGTAATTTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGAACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCgg                                                                                               <  1:942168/150‑1 (MQ=38)                                                             gggCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTa                                                                                          >  2:1416406/1‑150 (MQ=255)                                                                  acgggTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCcgc                                                                                     >  1:1158914/3‑150 (MQ=255)                                                                     ggTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGaa                                                                                  <  1:948592/150‑1 (MQ=255)                                                                            ggCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTa                                                                           <  1:1835493/150‑1 (MQ=255)                                                                             gCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAg                                                                          >  2:559751/1‑150 (MQ=255)                                                                               cGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCg                                                                        <  1:364593/150‑1 (MQ=255)                                                                               cGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCg                                                                        >  1:1025530/1‑150 (MQ=255)                                                                                      gATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGcaca                                                                                                                                           <  1:209431/76‑1 (MQ=255)                                                                                      gATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGcaca                                                                                                                                           >  2:209431/1‑76 (MQ=255)                                                                                        ttttATCGTCGAGCGCGGGATGCTGGGGATGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCCGTGGCTGGCACCGACTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGcaaca                                                               >  2:996887/1‑150 (MQ=255)                                                                                                tcgAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAAcgg                                                       >  1:1347087/1‑150 (MQ=255)                                                                                                  gAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAAcggcg                                                     >  1:1251514/1‑150 (MQ=255)                                                                                                   aGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTATGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAAcggcgg                                                    >  2:1710388/1‑150 (MQ=255)                                                                                                     cgcgGGATGCGGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGt                                                  <  2:1379654/150‑1 (MQ=255)                                                                                                      gcgGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTg                                                 <  2:402336/150‑1 (MQ=255)                                                                                                            tGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAAtt                                           <  1:1416406/150‑1 (MQ=255)                                                                                                              cTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTgg                                         >  1:1355538/1‑150 (MQ=255)                                                                                                              cTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGACATGCCCGCACaga                                                                                                                                         >  2:1177125/1‑54 (MQ=25)                                                                                                              cTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGACATGCCCGCACaga                                                                                                                                         <  1:1177125/54‑1 (MQ=25)                                                                                                                        gAGGCGGACCGACCGAGGTCCCAGCGAGGGCCTGAAGGCACAGAGGGCGACGCGGTTTCTGTCAGCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGggc                               <  2:116056/150‑1 (MQ=18)                                                                                                                             tGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTAAACCCGCGaa                                                                                  <  2:119129/94‑1 (MQ=255)                                                                                                                             tGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTAAACCCGCGaa                                                                                  >  1:119129/1‑94 (MQ=255)                                                                                                                              gACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGGAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCct                         <  1:141759/150‑1 (MQ=255)                                                                                                                              gACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCct                         >  1:1351318/1‑150 (MQ=255)                                                                                                                                   aTCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTcg                    >  1:1768957/1‑150 (MQ=255)                                                                                                                                      gAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTcgcg                  >  1:1029227/1‑149 (MQ=255)                                                                                                                                      gAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTcgcg                  <  2:1029227/149‑1 (MQ=255)                                                                                                                                       aGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCggg                >  1:736839/1‑150 (MQ=255)                                                                                                                                         gtctctGTGCGGACATGCCCGCACAGAGTCCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTa              >  2:913982/3‑150 (MQ=255)                                                                                                                                          tctctGTGCGGACATGCCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGACTACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTaa             >  2:331534/2‑150 (MQ=255)                                                                                                                                          aCTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCGCGACCGAACTGCGGGTGGGAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTaa             >  2:989361/1‑150 (MQ=37)                                                                                                                                            tctGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAAc           >  2:441552/1‑150 (MQ=255)                                                                                                                                             atgtgCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAAcc          <  1:1710388/149‑1 (MQ=255)                                                                                                                                               gtgCGGGCATGTCCGCACAGAGACCCACGCGGTGGCCGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCCGGGCCGGCCTCTTCGCGGGTAAACCCg        >  2:270127/1‑150 (MQ=255)                                                                                                                                                tgCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGc       <  2:758681/150‑1 (MQ=255)                                                                                                                                                tgCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGc       <  2:1158914/150‑1 (MQ=255)                                                                                                                                                 gCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCt      <  1:1679424/150‑1 (MQ=255)                                                                                                                                                   gggCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTcc    >  2:1448258/1‑150 (MQ=255)                                                                                                                                                     gCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTa  >  1:893445/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                      GGAGCGCGACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTA  >  NC_002947/3252387‑3252684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑