Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,252,540	T→C	7.9%	intergenic (+27/+216)	ureG → / ← PP_2851	urease accessory protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,252,540	0	T	C	7.9%	174.5 / 4.5	65	intergenic (+27/+216)	ureG/PP_2851	urease accessory protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/27);  new base C (3/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01

GACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGG  >  NC_002947/3252394‑3252689                                                                                                                                                   |                                                                                                                                                      gACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCGACCTGAAGAAGGGCGAGGGGTTGCAGTCCGTGAGGGATGTGATCCACCAGCGCGGGCTGCTGGGGGGGCGAGGATGACAGATCGAGGACTCTGTGCGGGCATGTccc                                                                                                                                                    >  2:731135/1‑149 (MQ=255) gACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCg                                                                                                                                                    >  2:1661676/1‑150 (MQ=255)     cccAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGcaca                                                                                                                                                <  1:534128/150‑1 (MQ=255)     cccAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGcaca                                                                                                                                                <  1:670062/150‑1 (MQ=255)       cAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACaga                                                                                                                                              >  2:1683762/1‑150 (MQ=255)       cAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGACATGCCCGCACaga                                                                                                                                              <  1:435921/150‑1 (MQ=255)          ggtccagggCCGCTGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGAc                                                                                                                                           >  2:58241/8‑150 (MQ=255)          cgcATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGAc                                                                                                                                           <  1:1599666/150‑1 (MQ=255)                       tggggCCCTGGACCTTCAGCAGCGTGGAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGAGCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCCACGCGGtggct                                                                                                                              <  2:705977/147‑1 (MQ=255)                       aGCGGCCCTGAGCCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGtggct                                                                                                                              <  1:762699/150‑1 (MQ=255)                             ccTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCg                                                                                                                                                    >  1:772864/1‑122 (MQ=255)                             ccTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCg                                                                                                                                                    <  2:772864/122‑1 (MQ=255)                               tGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCgg                                                                                                                      >  2:364593/1‑150 (MQ=255)                                   ccTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGAATAGATGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGctct                                                                                                                  >  1:116056/1‑150 (MQ=255)                                    cTTCAGCAACCTGAAGCAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTg                                                                                                                 <  1:176292/150‑1 (MQ=255)                                    cTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTg                                                                                                                 >  2:1362548/1‑150 (MQ=255)                                     ttCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGc                                                                                                                >  1:758681/1‑150 (MQ=255)                                     ttCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGc                                                                                                                <  1:600248/150‑1 (MQ=255)                                       cAGCAACCCGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCg                                                                                                              <  2:1044149/150‑1 (MQ=255)                                        aGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCgg                                                                                                             >  2:824672/1‑150 (MQ=255)                                            aCCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGtt                                                                                                         >  2:141759/1‑150 (MQ=255)                                                 aagaagGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCgg                                                                                                    <  1:938375/150‑1 (MQ=255)                                                 aagaagGGCGAGGGGTTGCACGCTCTGGTAATTTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGAACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCgg                                                                                                    <  1:942168/150‑1 (MQ=38)                                                      gggCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTa                                                                                               >  2:1416406/1‑150 (MQ=255)                                                           acgggTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCcgc                                                                                          >  1:1158914/3‑150 (MQ=255)                                                              ggTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGaa                                                                                       <  1:948592/150‑1 (MQ=255)                                                                     ggCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTa                                                                                <  1:1835493/150‑1 (MQ=255)                                                                      gCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAg                                                                               >  2:559751/1‑150 (MQ=255)                                                                        cGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCg                                                                             <  1:364593/150‑1 (MQ=255)                                                                        cGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCg                                                                             >  1:1025530/1‑150 (MQ=255)                                                                               gATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGcaca                                                                                                                                                <  1:209431/76‑1 (MQ=255)                                                                               gATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGcaca                                                                                                                                                >  2:209431/1‑76 (MQ=255)                                                                                 ttttATCGTCGAGCGCGGGATGCTGGGGATGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCCGTGGCTGGCACCGACTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGcaaca                                                                    >  2:996887/1‑150 (MQ=255)                                                                                         tcgAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAAcgg                                                            >  1:1347087/1‑150 (MQ=255)                                                                                           gAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAAcggcg                                                          >  1:1251514/1‑150 (MQ=255)                                                                                            aGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTATGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAAcggcgg                                                         >  2:1710388/1‑150 (MQ=255)                                                                                              cgcgGGATGCGGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGt                                                       <  2:1379654/150‑1 (MQ=255)                                                                                               gcgGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTg                                                      <  2:402336/150‑1 (MQ=255)                                                                                                     tGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAAtt                                                <  1:1416406/150‑1 (MQ=255)                                                                                                       cTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTgg                                              >  1:1355538/1‑150 (MQ=255)                                                                                                       cTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGACATGCCCGCACaga                                                                                                                                              <  1:1177125/54‑1 (MQ=25)                                                                                                       cTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGACATGCCCGCACaga                                                                                                                                              >  2:1177125/1‑54 (MQ=25)                                                                                                                 gAGGCGGACCGACCGAGGTCCCAGCGAGGGCCTGAAGGCACAGAGGGCGACGCGGTTTCTGTCAGCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGggc                                    <  2:116056/150‑1 (MQ=18)                                                                                                                      tGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTAAACCCGCGaa                                                                                       >  1:119129/1‑94 (MQ=255)                                                                                                                      tGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTAAACCCGCGaa                                                                                       <  2:119129/94‑1 (MQ=255)                                                                                                                       gACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGGAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCct                              <  1:141759/150‑1 (MQ=255)                                                                                                                       gACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCct                              >  1:1351318/1‑150 (MQ=255)                                                                                                                            aTCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTcg                         >  1:1768957/1‑150 (MQ=255)                                                                                                                               gAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTcgcg                       <  2:1029227/149‑1 (MQ=255)                                                                                                                               gAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTcgcg                       >  1:1029227/1‑149 (MQ=255)                                                                                                                                aGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCggg                     >  1:736839/1‑150 (MQ=255)                                                                                                                                  gtctctGTGCGGACATGCCCGCACAGAGTCCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTa                   >  2:913982/3‑150 (MQ=255)                                                                                                                                   tctctGTGCGGACATGCCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGACTACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTaa                  >  2:331534/2‑150 (MQ=255)                                                                                                                                   aCTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCGCGACCGAACTGCGGGTGGGAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTaa                  >  2:989361/1‑150 (MQ=37)                                                                                                                                     tctGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAAc                >  2:441552/1‑150 (MQ=255)                                                                                                                                      atgtgCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAAcc               <  1:1710388/149‑1 (MQ=255)                                                                                                                                        gtgCGGGCATGTCCGCACAGAGACCCACGCGGTGGCCGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCCGGGCCGGCCTCTTCGCGGGTAAACCCg             >  2:270127/1‑150 (MQ=255)                                                                                                                                         tgCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGc            <  2:758681/150‑1 (MQ=255)                                                                                                                                         tgCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGc            <  2:1158914/150‑1 (MQ=255)                                                                                                                                          gCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCt           <  1:1679424/150‑1 (MQ=255)                                                                                                                                            gggCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTcc         >  2:1448258/1‑150 (MQ=255)                                                                                                                                              gCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTa       >  1:893445/1‑150 (MQ=255)                                                                                                                                                aTGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACg     >  2:1423135/1‑150 (MQ=255)                                                                                                                                                  gTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACggg   <  2:1347087/150‑1 (MQ=255)                                                                                                                                                  gTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACggg   <  1:371286/150‑1 (MQ=255)                                                                                                                                                   tCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACgggg  <  1:559751/150‑1 (MQ=255)                                                                                                                                                   tCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACgggg  >  1:284261/1‑150 (MQ=255)                                                                                                                                                   tCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACgggg  >  1:283271/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                      GACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGG  >  NC_002947/3252394‑3252689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑