Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,380,750 C→G 6.1% Q127E (CAG→GAG)  tnpT‑I → cointegrate resolution protein T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,380,7500CG 6.1% 303.3 / 8.1 100Q127E (CAG→GAG) tnpT‑Icointegrate resolution protein T
Reads supporting (aligned to +/- strand):  ref base C (45/47);  new base G (2/4);  total (48/52)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 101 total aligned reads displayed.

ATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTGGAGGAAAAGCATC  >  NC_002947/3380601‑3380897
                                                                                                                                                         |                                                                                                                                                     
aTCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCACCGGAGAGCGAACAACTACAGCGTGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTc                                                                                                                                                       >  1:1179854/1‑150 (MQ=255)
   aGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAgg                                                                                                                                                    <  1:270031/150‑1 (MQ=255)
      tAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTg                                                                                                                                                 >  1:222009/1‑150 (MQ=255)
      tAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTg                                                                                                                                                 >  2:1145234/1‑150 (MQ=255)
          ggaggaTGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCa                                                                                                                                             <  1:277848/150‑1 (MQ=255)
           gaggaTGCGCAGGCCACCGTGGCGCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAg                                                                                                                                            >  2:944004/1‑150 (MQ=255)
             ggaTGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTNGAAAGTCAGAG‑‑‑CACCTGTCTCACGGAACA‑GCCTGAAGCTGTTccgtg                                                                                                                                          <  1:250138/150‑5 (MQ=255)
             ggaTGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCa                                                                                                                                          >  2:1571233/1‑150 (MQ=255)
              gaTGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCCTGAAGCTGTTccgtga                                                                                                                                         <  1:504196/150‑6 (MQ=255)
              gaTGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGACTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCCTGAAGCTGTTccgtga                                                                                                                                         <  2:1008510/150‑6 (MQ=255)
                   gcAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGc                                                                                                                                           >  2:55333/1‑143 (MQ=255)
                   gcAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGc                                                                                                                                           <  1:55333/143‑1 (MQ=255)
                       gCCACCGTGGCCCAGGGGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTAcag                                                                                                                               <  2:974829/150‑1 (MQ=255)
                       gCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAg‑c                                                                                                                                      <  2:1683040/143‑1 (MQ=255)
                       gCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAg‑c                                                                                                                                      >  1:1683040/1‑143 (MQ=255)
                        ccACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTAcagc                                                                                                                              >  2:1816740/1‑150 (MQ=255)
                         cACCGTCACCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTAcagca                                                                                                                             >  2:652137/1‑150 (MQ=255)
                           ccGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAgg                                                                                                                           <  1:1158017/150‑1 (MQ=255)
                            cGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTTAGGCTGtt                                                                                                                                               >  1:1463206/1‑130 (MQ=255)
                            cGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTTAGGCTGtt                                                                                                                                               <  2:1463206/130‑1 (MQ=255)
                                  ccAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACCGCAGGAAcagca                                                                                                                    <  1:1019575/150‑1 (MQ=255)
                                  ccAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAAcagca                                                                                                                    <  2:833963/150‑1 (MQ=255)
                                   cAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAAcagcag                                                                                                                   <  1:1816740/150‑1 (MQ=255)
                                       aGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAAc                                                                                                               <  2:806986/150‑1 (MQ=255)
                                        gcgcGAACAACTACAACGCGCACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACg                                                                                                              >  1:1029764/1‑150 (MQ=255)
                                            gAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGACACGCCaa                                                                                                          >  2:1039846/1‑150 (MQ=255)
                                            gAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCCTGAAGCTGTTccgt                                                                                                                                           <  2:786875/118‑4 (MQ=255)
                                            gAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCCTGAAGCTGTTccgt                                                                                                                                           >  1:786875/1‑115 (MQ=255)
                                              acaacTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAAGAGCAGCAACGCCAACa                                                                                                        >  2:968913/1‑150 (MQ=255)
                                               caacTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAg                                                                                                       >  2:603583/1‑150 (MQ=255)
                                                aacTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCa                                                                                                                                             <  1:1531296/112‑1 (MQ=255)
                                                aacTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCa                                                                                                                                             >  2:1531296/1‑112 (MQ=255)
                                                   tACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTAcag                                                                                                                               >  1:1141445/1‑122 (MQ=255)
                                                   tACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTAcag                                                                                                                               <  2:1141445/122‑1 (MQ=255)
                                                   tACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCg                                                                                                   >  1:136294/1‑150 (MQ=255)
                                                   tACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCg                                                                                                   >  2:248155/1‑150 (MQ=255)
                                                   tACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCg                                                                                                   >  2:736408/1‑150 (MQ=255)
                                                     cAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAg                                                                                                 <  1:652137/150‑1 (MQ=255)
                                                      aaCGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAgg                                                                                                >  2:1372174/1‑150 (MQ=255)
                                                      aaCGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAgg                                                                                                >  1:1626144/1‑150 (MQ=255)
                                                      aaCGCGAACGGCTCGATTACCAGAACAAAGGTGAGCTGGGCGAAAGCTTAAGCCAGGAGTTGGAAAGTTAGAGACACAGCAAACTTA‑‑GTTCATGC‑‑GAGACATTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCACCGGCAACAGACCGAgg                                                                                                >  1:897146/1‑150 (MQ=255)
                                                              cGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAAt                                                                                        <  2:136294/150‑1 (MQ=255)
                                                              cGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAAt                                                                                        <  2:1029764/150‑1 (MQ=255)
                                                                 cTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCGGT‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGcc                                                                                     <  1:987837/150‑1 (MQ=255)
                                                                 cTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGaa                                                                                                                         <  1:1488409/114‑1 (MQ=255)
                                                                 cTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGaa                                                                                                                         >  2:1488409/1‑114 (MQ=255)
                                                                 cTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGtt                                                                                              >  1:1828485/1‑141 (MQ=255)
                                                                 cTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGtt                                                                                              >  1:1826385/1‑141 (MQ=255)
                                                                 cTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGtt                                                                                              <  2:1826385/141‑1 (MQ=255)
                                                                 cTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGtt                                                                                              <  2:1828485/141‑1 (MQ=255)
                                                                 cTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGGGAATGcc                                                                                     <  1:944004/150‑1 (MQ=255)
                                                                  tCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGccc                                                                                    >  2:8152/1‑150 (MQ=255)
                                                                        aCCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTg‑g                                                                             <  1:736408/150‑1 (MQ=255)
                                                                         ccAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑gc                                                                            >  1:600047/1‑150 (MQ=255)
                                                                         ccAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑gc                                                                            <  1:1412691/150‑1 (MQ=255)
                                                                          cAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑gcg                                                                           >  1:1746351/1‑150 (MQ=255)
                                                                             aaCCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAg                                                                        >  2:1565819/1‑150 (MQ=255)
                                                                               ccAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGc                                                                      <  2:1179854/150‑1 (MQ=255)
                                                                                cAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑gc                                                                            >  2:1903958/1‑143 (MQ=255)
                                                                                cAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑gc                                                                            <  1:1903958/143‑1 (MQ=255)
                                                                                       ggCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCg                                                              >  2:1070999/1‑150 (MQ=255)
                                                                                        gCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGTCCGAGGTTTAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGa                                                             <  1:1571233/150‑1 (MQ=255)
                                                                                          tGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTGACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATc                                                           >  1:1826775/1‑150 (MQ=255)
                                                                                           ggCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCa                                                          <  2:897146/150‑1 (MQ=255)
                                                                                              cGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCagga                                                       >  2:513374/1‑150 (MQ=255)
                                                                                                      gAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGGGGTGCGcc                                               >  1:1514667/1‑150 (MQ=255)
                                                                                                         tCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTAAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGc                                            <  1:749765/150‑1 (MQ=255)
                                                                                                          ccAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCa                                           <  1:489472/150‑1 (MQ=255)
                                                                                                            agcagTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAAcagc                                                                                                                     >  2:11605/1‑75 (MQ=255)
                                                                                                            agcagTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAAcagc                                                                                                                     <  1:11605/75‑1 (MQ=255)
                                                                                                            agcagTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAgg                                         >  2:182993/1‑150 (MQ=255)
                                                                                                               agTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAg                                                                                                 <  1:1323122/92‑1 (MQ=255)
                                                                                                               agTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAg                                                                                                 >  2:1323122/1‑92 (MQ=255)
                                                                                                               agTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATc                                      <  1:8152/150‑1 (MQ=255)
                                                                                                               agTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATc                                      <  1:1212651/150‑1 (MQ=255)
                                                                                                                           agag‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCCAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCaaa                          >  2:635624/1‑150 (MQ=255)
                                                                                                                           agag‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCaaa                          >  1:484754/1‑150 (MQ=255)
                                                                                                                            gag‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATTCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAAt                         >  1:408997/1‑150 (MQ=255)
                                                                                                                             ag‑‑‑cagcGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAAtt                        <  2:614203/150‑1 (MQ=255)
                                                                                                                              g‑‑‑cagcGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATtc                       <  1:1509078/150‑1 (MQ=255)
                                                                                                                                      gggCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCATGCTAACGGCGATCAGGAGGTgc                                                  >  2:1360106/1‑118 (MQ=255)
                                                                                                                                      gggCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCATGCTAACGGCGATCAGGAGGTgc                                                  <  1:1360106/118‑1 (MQ=255)
                                                                                                                                      gggCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATtcgctcg                  >  1:336491/1‑150 (MQ=255)
                                                                                                                                          tCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCa                                           <  1:552080/121‑1 (MQ=255)
                                                                                                                                          tCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCa                                           >  2:552080/1‑121 (MQ=255)
                                                                                                                                          tCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTgg              >  2:1872208/1‑150 (MQ=255)
                                                                                                                                           cACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAGTGGCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTgga             <  1:603583/150‑1 (MQ=255)
                                                                                                                                           cACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTgga             <  1:221179/150‑1 (MQ=255)
                                                                                                                                           cACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTgga             <  1:1872208/150‑1 (MQ=255)
                                                                                                                                           cACGGAACA‑GCTTCAGGCTGTTCAGAACGAAGCAAC‑CTATTAACATCAACAACGGCAACAGACCGAGGTTGAATATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTgga             <  1:423666/150‑1 (MQ=255)
                                                                                                                                            aCGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTggag            <  2:222009/150‑1 (MQ=255)
                                                                                                                                            aCGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTggag            <  2:615268/150‑1 (MQ=255)
                                                                                                                                            aCGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTggag            <  1:1372174/150‑1 (MQ=255)
                                                                                                                                              ggAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAg                                                                                                 <  1:981826/64‑1 (MQ=255)
                                                                                                                                                aaCA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGAGCAGGAGGTACGACTATGAGAGCGCGACAGCCAAATTCGCTCGCTGGAGGaaa        >  2:1893755/1‑150 (MQ=255)
                                                                                                                                                   a‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTggag            <  1:285841/143‑1 (MQ=255)
                                                                                                                                                   a‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTggag            >  2:285841/1‑143 (MQ=255)
                                                                                                                                                   a‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTGGAGGAAAAGc     >  1:250704/1‑150 (MQ=255)
                                                                                                                                                       ttCAGGCTGTTCAGCCAG‑CGCTGAAGCTACTACAGCAGCAACTCCAACAGACCGAGGTTG‑GATTTACTCCATGTTCTCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTGGAGGAAAAGCATc  <  1:513374/150‑1 (MQ=255)
                                                                                                                                                       ttCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTGGAGGAAAAGCATc  <  2:1028627/150‑1 (MQ=255)
                                                                                                                                                         |                                                                                                                                                     
ATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG‑‑‑CAGCGGGCTCACGGAACA‑GCTTCAGGCTGTTCAGCAAG‑CGCTACAGCAGGAACAGCAGCAACGCCAACAGACCGAGGTTGAGAATGCCCGCTTG‑GCGCAGGCTAACGGCGATCAGGAGGTGCGCCTGCAGGATCGCGACAGCCAAATTCGCTCGCTGGAGGAAAAGCATC  >  NC_002947/3380601‑3380897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑