Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,435,965 G→A 5.7% Q278Q (CAG→CAA)  gpJ → baseplate/tail fiber protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,435,9650GA 5.7% 199.2 / 2.4 70Q278Q (CAG→CAA) gpJbaseplate/tail fiber protein
Reads supporting (aligned to +/- strand):  ref base G (25/41);  new base A (2/2);  total (27/43)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

CGGCCCCGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAGGTGGTGACATG  >  NC_002947/3435816‑3436111
                                                                                                                                                     |                                                                                                                                                  
cgGCCCCGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAg                                                                                                                                                    <  1:684478/150‑1 (MQ=255)
   ccccGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTg                                                                                                                                                 <  1:1216525/150‑1 (MQ=255)
    cccGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCAGTCGCCTGCAGGGGCAGTTGc                                                                                                                                                <  1:254782/150‑1 (MQ=255)
     ccGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGcc                                                                                                                                               <  1:328117/150‑1 (MQ=255)
     ccGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGcc                                                                                                                                               >  2:1247424/1‑150 (MQ=255)
      cgcgTGGCCGCCGGCGTACCGACTGAGTTCAGGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGccc                                                                                                                                              <  1:1825836/150‑1 (MQ=255)
      cgcgTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGGGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGccc                                                                                                                                              <  1:1097696/150‑1 (MQ=255)
        cgTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGcaact                                                                                                                                            <  2:1270085/150‑5 (MQ=255)
           ggCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAACTGCCCCTgca                                                                                                                                         <  2:904937/150‑3 (MQ=255)
            gccgccGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCGACAGGGGCAACTGCCCCTgcag                                                                                                                                        <  2:148941/150‑4 (MQ=255)
                       aCCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGc                                                                                                                             <  1:1447657/150‑1 (MQ=255)
                          gACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCcgc                                                                                                                          <  1:1764024/150‑1 (MQ=255)
                             tGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGtt                                                                                                                       >  1:996618/1‑150 (MQ=255)
                               aGTTCAAGGTGGAGGCCGGGCTGGACACCGGCGGAGGGGTAGGCCCTGGCGTGATGAAGGGCATGGCGCGGTCGCGCGTAAACCCGGCCATAGCAGAGACGCGTTGCCTGGAGCGGCAGGGGCCGCTGTCGGAGAGCTGTGCCGCTTTac                                                                                                                     <  2:799354/150‑1 (MQ=255)
                                 ttCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTacac                                                                                                                   >  1:74356/1‑150 (MQ=255)
                                 ttCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGCGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTacac                                                                                                                   <  2:1693617/150‑1 (MQ=255)
                                    aaGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTa                                                                                                                >  1:191302/1‑150 (MQ=255)
                                     aGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTaa                                                                                                               <  2:1745578/150‑1 (MQ=255)
                                         ggAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACGTGTAACgcg                                                                                                           <  1:1538781/150‑3 (MQ=255)
                                                   cTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCGCAGCACAGAGTGTCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGc                                                                                                 >  1:1482872/1‑150 (MQ=255)
                                                   cTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCGTGACTGCGGCCCTTGCCGGCGCCCGTGGCATGGAGGGGCGGTTGCTCGTGTCTGCCATCCAGGCCGCTTTACACGTAACGGCGATTCGTAGc                                                                                                 <  2:497319/150‑1 (MQ=255)
                                                   cTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGc                                                                                                 <  1:1493744/150‑1 (MQ=255)
                                                   cTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAACGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGc                                                                                                 <  1:1147949/150‑1 (MQ=255)
                                                     gCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGt                                                                                               <  2:1657922/150‑1 (MQ=255)
                                                         aTCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGAcc                                                                                           >  2:818842/1‑150 (MQ=255)
                                                              ggAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGaa                                                                                      <  2:1460436/150‑1 (MQ=255)
                                                              ggAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGaa                                                                                      <  2:996618/150‑1 (MQ=255)
                                                               gAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAg                                                                                     >  2:1146447/1‑150 (MQ=255)
                                                               gAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAg                                                                                     >  2:1655373/1‑150 (MQ=255)
                                                               gAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAg                                                                                     <  1:1003168/150‑1 (MQ=255)
                                                               gAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAg                                                                                     >  2:1748306/1‑150 (MQ=255)
                                                                     ccAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATc                                                                                 <  2:1209181/148‑1 (MQ=255)
                                                                     ccAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATc                                                                                 >  1:1209181/1‑148 (MQ=255)
                                                                       aGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGc                                                                             >  2:456548/1‑150 (MQ=255)
                                                                            cTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCg                                                                        >  2:661139/1‑150 (MQ=255)
                                                                                  tggtgAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCa                                                                               <  1:1391709/137‑1 (MQ=255)
                                                                                  tggtgAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCa                                                                               >  2:1391709/1‑137 (MQ=255)
                                                                                   ggtgAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCCGTAATCCTTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGggtggt                                                                 <  1:1645590/150‑1 (MQ=255)
                                                                                   ggtgAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGggtggt                                                                 <  1:1341507/150‑1 (MQ=255)
                                                                                     tgAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGgtgt                                                               <  1:1655373/150‑1 (MQ=255)
                                                                                       aaGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGgtgtgt                                                             <  2:191302/150‑1 (MQ=255)
                                                                                       aaGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGgtgtgt                                                             <  2:1100722/150‑1 (MQ=255)
                                                                                        aGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGccc                                                                                                                                              <  1:1554649/68‑1 (MQ=255)
                                                                                        aGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGccc                                                                                                                                              >  2:1554649/1‑68 (MQ=255)
                                                                                         ggCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGgtgtgt                                                             >  1:1373797/1‑148 (MQ=255)
                                                                                         ggCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGgtgtgt                                                             <  2:1373797/148‑1 (MQ=255)
                                                                                              cGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGc                                                      <  1:456548/150‑1 (MQ=255)
                                                                                                            cTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGt                                        >  2:1475324/1‑150 (MQ=255)
                                                                                                            cTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGt                                        >  1:534657/1‑150 (MQ=255)
                                                                                                             tGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGtt                                       <  1:1060698/150‑1 (MQ=255)
                                                                                                                 tGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCAc                                   <  1:818842/150‑1 (MQ=255)
                                                                                                                     gCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTg                                      >  2:1873072/1‑143 (MQ=255)
                                                                                                                     gCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTg                                      <  1:1873072/143‑1 (MQ=255)
                                                                                                                            cAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGc                        <  1:1247424/150‑1 (MQ=255)
                                                                                                                             agtaGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCt                       <  1:661139/147‑1 (MQ=255)
                                                                                                                                aGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTCCGCTGAc                    <  2:74356/150‑1 (MQ=255)
                                                                                                                                 gCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACg                   >  1:1032289/1‑150 (MQ=255)
                                                                                                                                    cGTCGCCTGTAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGtt                                       >  2:1275171/1‑127 (MQ=255)
                                                                                                                                    cGTCGCCTGTAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGtt                                       <  1:1275171/127‑1 (MQ=255)
                                                                                                                                      tCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGaa              >  2:577033/1‑150 (MQ=255)
                                                                                                                                        gCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGAAGTCGAAgg            <  2:376914/150‑1 (MQ=255)
                                                                                                                                          cgacAGGGGCAACTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAggtg          >  1:221505/4‑150 (MQ=255)
                                                                                                                                          cTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAggtg          <  1:14530/150‑1 (MQ=255)
                                                                                                                                           tGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAggtgg         <  1:1154799/150‑1 (MQ=255)
                                                                                                                                           tGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAggtgg         <  1:1155014/150‑1 (MQ=255)
                                                                                                                                           tGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAggtgg         >  1:1019421/1‑150 (MQ=255)
                                                                                                                                           gacAGGGGCAACTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTCGTGTGTGATAAGCGGCATTACCCCAGCTGCACGTCGGTTACGCTGACGTCGAcggcgg         >  2:1104271/3‑150 (MQ=255)
                                                                                                                                             cAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAGgtggtg       >  2:38254/1‑150 (MQ=255)
                                                                                                                                                 ggCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCg                               <  2:1463377/122‑1 (MQ=255)
                                                                                                                                                 ggCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCg                               >  1:1463377/1‑122 (MQ=255)
                                                                                                                                                  gCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAGGTGGTGACATg  >  1:672573/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGGCCCCGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAGGTGGTGACATG  >  NC_002947/3435816‑3436111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑