Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,532,796 C→T 9.2% G144S (GGC→AGC)  PP_3121 ← transcriptional regulator, LysR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,532,7960CT 9.2% 169.0 / 6.1 66G144S (GGC→AGC) PP_3121transcriptional regulator, LysR family
Reads supporting (aligned to +/- strand):  ref base C (29/30);  new base T (3/3);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

GCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCAACAGGTTGCCGGCAAAAGCCGGCATGGCTGCCACC  >  NC_002947/3532648‑3532944
                                                                                                                                                    |                                                                                                                                                    
gCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGccc                                                                                                                                                     >  1:1711960/1‑150 (MQ=255)
 ccACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCa                                                                                                                                                    >  2:936770/1‑150 (MQ=255)
 ccACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCa                                                                                                                                                    <  2:81021/150‑1 (MQ=255)
   aCGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGt                                                                                                                                                  >  2:395487/1‑150 (MQ=255)
    cGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGtt                                                                                                                                                 >  1:1156354/1‑150 (MQ=255)
    cGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGtt                                                                                                                                                 <  1:401706/150‑1 (MQ=255)
    cGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGtt                                                                                                                                                 >  1:1425878/1‑150 (MQ=255)
        aTCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGAc                                                                                                                                             <  2:1614643/150‑1 (MQ=255)
           ttCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGAcgcg                                                                                                                                          >  1:1329459/1‑150 (MQ=255)
            tCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACgcgg                                                                                                                                         <  1:1879551/150‑1 (MQ=255)
                             ccAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACCACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGAAGCCCAGGTCGCCGCGGCGATGGCGTACCAGTTc                                                                                                                        >  2:309524/1‑150 (MQ=255)
                             ccAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTc                                                                                                                        <  1:395487/150‑1 (MQ=255)
                              cAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTcc                                                                                                                       >  1:1921543/1‑150 (MQ=255)
                               aGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCt                                                                                                                      <  2:1215125/150‑1 (MQ=255)
                                      cttgggCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTg                                                                                                               >  1:1371795/4‑150 (MQ=255)
                                      cTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTg                                                                                                               >  1:1210502/1‑150 (MQ=255)
                                        ggggGAGCAGTGTCAACGGCCAGTCGGCGGGCACTACGTCCACGAAGCGCTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGCTGGCGTAACAGTTCCTGTACCTGtt                                                                                                             <  2:688230/150‑1 (MQ=255)
                                        ggggCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGtt                                                                                                             <  2:1210502/150‑1 (MQ=255)
                                                        gggCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTg                                                                                             >  1:453779/1‑150 (MQ=255)
                                                         ggCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGc                                                                                            >  1:169999/1‑150 (MQ=255)
                                                                cggcggCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGa                                                                                     <  1:1594068/150‑1 (MQ=255)
                                                                    ggcCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGGGTACCAGTTCCTGTACCTGtt                                                                                                             <  2:1508381/122‑1 (MQ=255)
                                                                    ggcCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGtt                                                                                                             >  1:1508381/1‑122 (MQ=255)
                                                                     gcCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCTCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTg                                                                                >  2:280770/1‑150 (MQ=255)
                                                                     gcCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCTCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTg                                                                                >  2:280798/1‑150 (MQ=255)
                                                                     gcCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCTCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTg                                                                                >  2:280708/1‑150 (MQ=255)
                                                                      ccACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGa                                                                               >  2:1711944/1‑150 (MQ=255)
                                                                      ccACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGa                                                                               <  2:1450999/150‑1 (MQ=255)
                                                                          tACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGAt                                                                                                                                     <  2:59876/92‑1 (MQ=255)
                                                                          tACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGAt                                                                                                                                     >  1:59876/1‑92 (MQ=255)
                                                                           aCGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTg                                                                          <  2:1227667/150‑1 (MQ=255)
                                                                            cGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTgg                                                                         <  1:1003177/150‑1 (MQ=255)
                                                                             ggCAACGAAGCGGTCCATGTACAACGGGTGGATGTCCAGCCCATCGATGTTTTCCGGCTCGAAGCCGATGCCCAGTTCGACGGGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATTACGTCGTGCACGGGTACGTTTGCCTTggg                                                                        <  2:1553171/150‑1 (MQ=255)
                                                                             ggCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGCTGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTggg                                                                        <  1:1667790/150‑1 (MQ=255)
                                                                             ggCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTAGCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTggg                                                                        >  1:527146/1‑150 (MQ=255)
                                                                                aaCGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTa                                                                                                                   <  1:1472147/104‑1 (MQ=255)
                                                                                aaCGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTa                                                                                                                   >  2:1472147/1‑104 (MQ=255)
                                                                                 aCGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAg                                                                    >  1:451913/1‑150 (MQ=255)
                                                                                  cGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAgc                                                                   <  2:1156354/150‑1 (MQ=255)
                                                                                   gAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTg                                                                          >  1:769510/1‑142 (MQ=255)
                                                                                   gAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTg                                                                          <  2:769510/142‑1 (MQ=255)
                                                                                   gAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAgcg                                                                  >  1:1055591/1‑150 (MQ=255)
                                                                                    aaGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAgcgc                                                                 <  1:418683/150‑1 (MQ=255)
                                                                                      gCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTg                                                               >  2:1258028/1‑150 (MQ=255)
                                                                                         gTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCa                                                                                                                                                    >  1:1647030/1‑62 (MQ=255)
                                                                                         gTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCa                                                                                                                                                    <  2:1647030/62‑1 (MQ=255)
                                                                                            cATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGaaa                                                         <  2:1921543/150‑1 (MQ=255)
                                                                                             aTGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAac                                                        <  2:1425878/150‑1 (MQ=255)
                                                                                                    aCGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCCAAGCCGATGCACAGTTCGACGCTGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTTGGGTAGCGCTGGCGAAACACCTTGa                                                 <  1:881888/150‑1 (MQ=255)
                                                                                                            aaGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTTAGTGTAGGGGAGCGCTGGCGAAACACCTTGAGCGAGTgg                                         >  1:1039679/1‑150 (MQ=255)
                                                                                                              gTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGGTGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTgggg                                       >  2:929788/1‑150 (MQ=255)
                                                                                                               tCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGTTTTCGAAACACCTTGAGCGAGTGGGGc                                      >  1:1434771/1‑150 (MQ=255)
                                                                                                                 cAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCTCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCaa                                    <  1:280798/150‑1 (MQ=255)
                                                                                                                 cAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCTCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCaa                                    <  1:280708/150‑1 (MQ=255)
                                                                                                                 cAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCTCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCaa                                    <  1:280770/150‑1 (MQ=255)
                                                                                                                   gCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCAACa                                  >  2:866630/1‑150 (MQ=255)
                                                                                                                                 tCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCAACAGGTTGCCGGCaaaa                    >  1:1822068/1‑150 (MQ=255)
                                                                                                                                     gCTCGCAGCCGACGCCCAGTTCGACTCTGCGTTGACGTACCAGTTCCTGTACCTGTTCGTTGATCAAGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCAACAGGTTGCCGGCAAAAGCCg                <  1:882666/150‑1 (MQ=255)
                                                                                                                                     gCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCCCGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCAACAGGTTGCCGGCAAAAGCCg                <  1:883305/150‑1 (MQ=255)
                                                                                                                                        cGAAGCCGATGCCCAGTTCGGCGCGGCGATGGCGTACCAGTTACTGTACCGGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCAACAGGTTGCCGGCAAAAGCCGGCa             <  2:251325/150‑1 (MQ=255)
                                                                                                                                          aaGCCGATGCCCATTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTGCCTGTGTGCTTGATCAGTGGGTACTTTGACCTTGGATTAGAGCTGGCGAAACCCATTGTGCGAGTGGGGCAACAGGTTGCCGGCAAAAGCCGGCATg           <  1:896607/150‑1 (MQ=255)
                                                                                                                                           aGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCAACAGGTTGCCGGCAAAAGCCGGCATgg          >  1:940090/1‑150 (MQ=255)
                                                                                                                                           aGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCCTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCAACAGGTTGCCGGCAAAAGCCGGCATgg          >  2:66707/1‑150 (MQ=255)
                                                                                                                                                 tGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCAACAGGTTGCCGGCAAAAGCCGGCATGGCTGCCa    <  1:866630/150‑1 (MQ=255)
                                                                                                                                                 tGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCAACAGGTTGCCGGCAAAAGCCGGCATGGCTGCCa    <  2:1711960/150‑1 (MQ=255)
                                                                                                                                                  gCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTgggg                                       <  1:600935/114‑1 (MQ=255)
                                                                                                                                                  gCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTgggg                                       >  2:600935/1‑114 (MQ=255)
                                                                                                                                                   cccAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCAACAGGTTGCCGGCAAAAGCCGGCATGGCTGCCAcc  <  2:1866137/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCACGAAATCTTCGGCCATCAGCTGTGCCCAGCTGACCTGGGGCAGCAGTGCCAAGGGCGAGTCGGCGGCCACTACGGCAACGAAGCGGTCCATGTACAACGGGTGGAAGTCCAGCCCATCGATGTTATCCGGCTCGAAGCCGATGCCCAGTTCGACGCGGCGATGGCGTACCAGTTCCTGTACCTGTTCGTTGATCACGTCGTGCACCGTGACGTTGACCTTGGGGTAGCGCTGGCGAAACACCTTGAGCGAGTGGGGCAACAGGTTGCCGGCAAAAGCCGGCATGGCTGCCACC  >  NC_002947/3532648‑3532944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑