| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,534,805 | T→G | 10.4% | intergenic (+49/‑106) | atoB → / → PP_3124 | 3‑oxoacid CoA‑transferase subunit B/short chain fatty acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,534,805 | 0 | T | G | 10.4% | 142.1 / 8.1 | 57 | intergenic (+49/‑106) | atoB/PP_3124 | 3‑oxoacid CoA‑transferase subunit B/short chain fatty acid transporter |
| Reads supporting (aligned to +/- strand): ref base T (21/30); new base G (4/2); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 | |||||||||||
TTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCTTT > NC_002947/3534656‑3534949 | ttCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGt < 2:1826760/150‑1 (MQ=255) tCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTc < 1:682601/150‑1 (MQ=255) cTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCg < 1:482146/150‑1 (MQ=255) gcgAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTAc < 2:1060895/150‑1 (MQ=255) gcgAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGGGATCTCACCCTGAAGGCGCGGGTTTACCCGCGAGTGCGTCGGTAc > 1:196636/1‑150 (MQ=255) gagaCCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACAt < 2:1129562/150‑1 (MQ=255) agaCCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACAtt < 2:402317/150‑1 (MQ=255) aCCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCGGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑a > 1:631255/1‑150 (MQ=255) aCCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑a < 2:1839467/150‑1 (MQ=255) cTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACAtt < 2:495085/141‑1 (MQ=255) cTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACAtt > 1:495085/1‑141 (MQ=255) tGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCa < 1:1900151/150‑1 (MQ=255) gTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCgg < 2:1604114/147‑1 (MQ=255) gTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCgg > 1:1604114/1‑147 (MQ=255) tcGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCgg < 1:1909019/134‑1 (MQ=255) tcGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCgg > 2:1909019/1‑134 (MQ=255) tcGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTAc > 2:203416/1‑150 (MQ=255) aaaCTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGt < 1:1664765/91‑1 (MQ=255) aaaCTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGt > 2:1664765/1‑91 (MQ=255) aaCTGATCGTGGCGGATGATGTGAAGGAAATGACCTGCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCt < 2:519669/150‑1 (MQ=255) aaCTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTTCATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCt > 1:1755982/1‑150 (MQ=255) aaCTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCt < 1:203416/150‑1 (MQ=255) aCTGATCTTGCCGGATAATGTGAAAGAAATGACCTTCTAAGGCATGACGTGATCCCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACCTTG‑ACCTCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTg < 1:163566/150‑1 (MQ=38) aCTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTg < 1:1798053/150‑1 (MQ=255) aCTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTg > 2:1272057/1‑150 (MQ=255) tGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGc < 1:1210533/150‑1 (MQ=255) tgatgTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCAt < 2:564402/150‑1 (MQ=255) tgatgTGAAGGAAATGACCTTCTAAGGCATGACGTGATCCCGACCTGTGGGCGCGGGTTTACCCGCGAATGAATAATTTCATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCAt < 1:126546/150‑1 (MQ=255) tgtgAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCgg > 1:1433941/1‑74 (MQ=255) tgtgAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCgg < 2:1433941/74‑1 (MQ=255) gtgAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCa > 1:1181810/1‑150 (MQ=255) tgAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGAGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCgg > 1:526238/1‑95 (MQ=255) tgAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGAGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCgg < 2:526238/95‑1 (MQ=255) tgAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGCTTACCCGCGAATGCGGCGGTCAATGT‑ACCGACGCATTCGCgg < 1:898216/95‑1 (MQ=18) tgAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGCTTACCCGCGAATGCGGCGGTCAATGT‑ACCGACGCATTCGCgg > 2:898216/1‑95 (MQ=18) tgAAGGAAATGACCTCCTAAGGCATGACGTGACCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGCTATATTT‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCAt < 1:126145/150‑1 (MQ=39) ggAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGtttttt < 2:1354772/116‑1 (MQ=255) ggAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGtttttt > 1:1354772/1‑116 (MQ=255) aTGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTCTCTGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCaa < 1:416605/150‑1 (MQ=255) cTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCaaagatc > 1:5359/1‑145 (MQ=255) cTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATaaaa < 1:89070/150‑1 (MQ=255) aaGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAAt < 1:920217/150‑1 (MQ=255) gCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCa > 1:1711655/1‑150 (MQ=255) aCGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCaa < 1:1431450/132‑1 (MQ=255) aCGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCaa > 2:1431450/1‑132 (MQ=255) tcACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTg > 1:910601/1‑150 (MQ=255) aCCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGaa > 2:1430211/1‑150 (MQ=255) ccTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAAt > 2:1814931/1‑150 (MQ=255) cTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACa > 2:447072/1‑37 (MQ=255) cTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACa < 1:447072/37‑1 (MQ=255) tGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCtt < 2:939493/102‑1 (MQ=255) tGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCtt > 1:939493/1‑102 (MQ=255) ccGCGAATGCGGCGGT‑CAATGTACCGACGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCAt < 1:1245205/88‑1 (MQ=18) ccGCGAATGCGGCGGT‑CAATGTACCGACGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCAt > 2:1245205/1‑88 (MQ=18) ccGCGAATGCGGCGGT‑CAATGTACCGACGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgcg > 2:1266920/1‑150 (MQ=38) ccGCGAATGCGGCGGT‑CAATGTACCGACGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgcg > 2:1257761/1‑150 (MQ=38) cgcgAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCcgcgc > 1:622971/1‑150 (MQ=255) cgAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCgcgcgc < 2:213577/150‑1 (MQ=255) aTGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCttt < 1:1814931/150‑1 (MQ=255) aTGCGTCAGTAAATTG‑ACCTCAGTTTTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTTGATACGCCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCttt < 1:594358/150‑1 (MQ=255) | TTCTGCGCGAGACCGCGCCTGGGGTCAGCGTCGAGGAAATCATCGAGAAAACCGCCGGCAAACTGATCGTGCCGGATGATGTGAAGGAAATGACCTTCTAAGGCATGACGTGATCTCACCCTGTAGGCGCGGGTTTACCCGCGAATGCGTCGGTACATTG‑ACCGCCGCATTCGCGGGTAAGCCTGTTCCTACAGGTTTTTTGCGTTGCCTGGCAATACCGCTTCATCCCATAAAACCAATAAAAGGAATTCAACCGTGGCCGCTGAAATTCAAGACAGCCGCTCCGCGCGCTTT > NC_002947/3534656‑3534949 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |