Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,694,072	T→G	12.0%	R713R (CGA→CGC) ‡	ligD ←	DNA ligase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,694,072	0	T	G	12.0%	190.3 / 13.1	78	R713R (CGA→CGC) ‡	ligD	DNA ligase D
Reads supporting (aligned to +/- strand):  ref base T (31/37);  new base G (6/4);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGCGCCCTTGCCGTTGCGCAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCAGCGCAGGGTCAGGGTCCAGGT  >  NC_002947/3693924‑3694220                                                                                                                                                     |                                                                                                                                                      tAGCGCCCTTGCCGTTGCGCAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTc                                                                                                                                                      <  1:1541634/150‑1 (MQ=255) tAGCGCCCTTGCCGTTGCGCAGGTAGTAGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTc                                                                                                                                                      >  1:1372236/1‑150 (MQ=255)    cgcCCTTGCCGTTGCGCAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGcc                                                                                                                                                   >  1:1891269/1‑150 (MQ=255)     gcCCTTGCCGTTGCGCAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCg                                                                                                                                                  >  2:1566613/1‑150 (MQ=255)     gcCCTTGCCGTTGCGCAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCg                                                                                                                                                  >  2:36284/1‑150 (MQ=255)     gcCCTTGCCGTTGCGCAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCg                                                                                                                                                  <  1:805762/150‑1 (MQ=255)      cccTTGCCGTTGCGCAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCgg                                                                                                                                                 >  1:317234/1‑150 (MQ=255)       ccTTGCCGTTGCGCAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCCCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCggg                                                                                                                                                >  2:256333/1‑150 (MQ=255)        cTTGCCGTTGCGCAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGt                                                                                                                                               >  1:351190/1‑150 (MQ=255)        cTTGCCGTTGCGCAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGt                                                                                                                                               >  1:1602348/1‑150 (MQ=255)        cTTGCCGTTGCGCAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTCCGCCCCCGACACCGCGCTCAGGCGCTCCGGTCACAGCTTGGCCAGGTCTGTATCGATGGCCTGTCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGt                                                                                                                                               <  1:36284/150‑1 (MQ=255)              gTTGCGCAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAggg                                                                                                                                         >  1:1878447/1‑150 (MQ=255)                    cAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCGAGCCGGGtg                                                                                                                                              <  2:583643/139‑2 (MQ=255)                    cAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCGAGCCGGGtg                                                                                                                                              >  1:583643/1‑138 (MQ=255)                      ggTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATAGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCa                                                                                                                                 <  1:1339842/150‑1 (MQ=255)                         aGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑Gt                                                                                                                             <  1:874147/150‑1 (MQ=255)                           tCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCGCGCCGGGTCAAGGGCACAACCAC‑GTGa                                                                                                                           >  2:100136/1‑150 (MQ=255)                             gATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAAt                                                                                                                         >  1:808236/1‑150 (MQ=255)                             gATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAAt                                                                                                                         <  2:351190/150‑1 (MQ=255)                             gATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAAt                                                                                                                         >  1:1192353/1‑150 (MQ=255)                              aTGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATa                                                                                                                        >  1:1519387/1‑150 (MQ=255)                              aTGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCGAGCCGGGtgg                                                                                                                                             <  1:794837/130‑3 (MQ=255)                              aTGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCGAGCCGGGtgg                                                                                                                                             >  2:794837/1‑128 (MQ=255)                                gAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATAcc                                                                                                                      <  1:252346/150‑1 (MQ=255)                                 aaaaTGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATAccc                                                                                                                     <  2:1132879/150‑1 (MQ=255)                                   aaTGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCtt                                                                                                                   <  2:1746534/150‑1 (MQ=255)                                     tGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGc                                                                                                                 >  1:1203638/1‑150 (MQ=255)                                     tGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGc                                                                                                                 <  1:1095727/150‑1 (MQ=255)                                     tGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGc                                                                                                                 <  1:1397190/150‑1 (MQ=255)                                            cACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GGGAATACCCTTGCCACCACt                                                                                                          >  1:503225/1‑150 (MQ=255)                                             aCGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTg                                                                                                         <  2:1891269/150‑1 (MQ=255)                                               gcgATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGt                                                                                                       >  1:300764/1‑150 (MQ=255)                                               gcgATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGATTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAAACAC‑GTGAATACACTTCCGACTACTGGt                                                                                                       >  2:405929/1‑150 (MQ=255)                                                cgATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGtt                                                                                                      <  2:424461/150‑1 (MQ=255)                                                    ttttCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCa                                                                                                  <  1:861961/150‑1 (MQ=255)                                                     tttCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAg                                                                                                 <  1:184109/150‑1 (MQ=255)                                                            cccGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGACGGGTCAAGGG‑ACCACAACTGGTAACACCCATGCCACCACTTCTGGTCAGGAacaca                                                                                          >  2:496246/1‑149 (MQ=255)                                                             ccGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTGGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGaa                                                                                              >  1:1277753/1‑145 (MQ=255)                                                             ccGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTGGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGaa                                                                                              <  2:1277753/145‑1 (MQ=255)                                                                acacCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCa                                                                                      <  2:808236/150‑1 (MQ=255)                                                                  acCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCCTCCgg                                                                                    <  2:300764/150‑1 (MQ=255)                                                                   ccGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCa                                                                                                  >  2:992230/1‑135 (MQ=255)                                                                   ccGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCa                                                                                                  <  1:992230/135‑1 (MQ=255)                                                                   ccGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGc                                                                                   >  1:220419/1‑150 (MQ=255)                                                                    cgcgCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCCATGGATTTCAGCAAAACAGTCCGGcc                                                                                  >  2:870758/1‑150 (MQ=255)                                                                     gcgcTCAGGCGCGCCGGAACCAGATTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACAACTGGTTTTCAGGAACACCTTCAGGccc                                                                                 <  2:621055/150‑1 (MQ=255)                                                                             gcgcTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTc                                                                         <  2:1878447/150‑1 (MQ=255)                                                                                  ccGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCcagca                                                                    >  2:1863728/1‑150 (MQ=255)                                                                                    ggAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAgg                                                                  <  2:1372236/150‑1 (MQ=255)                                                                                          cgCTTGGCCAGGTAATTGTCGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCACGGGCACAACCAC‑GTTAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAgg                                                            <  2:189094/149‑1 (MQ=255)                                                                                             ttGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAgg                                                            <  1:1394310/147‑1 (MQ=255)                                                                                             ttGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAgg                                                            >  2:1394310/1‑147 (MQ=255)                                                                                               ggCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGCTCGCCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAg                                                       >  1:205453/1‑150 (MQ=255)                                                                                                gCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCtt                                                                                                                   >  1:505654/1‑89 (MQ=255)                                                                                                gCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCtt                                                                                                                   <  2:505654/89‑1 (MQ=255)                                                                                                   aGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCATGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTgg                                                   >  2:1485439/1‑150 (MQ=255)                                                                                                   aGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTgg                                                   <  1:1485439/150‑1 (MQ=255)                                                                                                   aGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGNTgg                                                   <  2:1192353/150‑1 (MQ=255)                                                                                                           gACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTc                                           <  2:3743/150‑1 (MQ=255)                                                                                                                               ccTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCa                       <  1:405929/150‑1 (MQ=255)                                                                                                                                cTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCAg                      >  2:706419/1‑150 (MQ=255)                                                                                                                                 ttGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAgg                                                                                    <  1:302344/87‑1 (MQ=255)                                                                                                                                 ttGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAgg                                                                                    >  2:302344/1‑87 (MQ=255)                                                                                                                                     ccTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCAGCGcag                 <  1:1566613/150‑1 (MQ=255)                                                                                                                                      cTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCAGCGcagg                >  1:1396076/1‑150 (MQ=255)                                                                                                                                          tCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCAGCGcagggtca            <  2:1810726/150‑1 (MQ=255)                                                                                                                                          cttgaCCCGGCGAGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAgg                          <  1:954549/132‑1 (MQ=255)                                                                                                                                          cttgaCCCGGCGAGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAgg                          >  2:954549/5‑136 (MQ=255)                                                                                                                                          cttgaCCCGGCGAGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCAGCGcagggtca            >  2:599915/5‑150 (MQ=255)                                                                                                                                          cgtgaCCCGGTTAGCCGGTTCAAGGGCACAACCAC‑GAGAATACCCTTGCCACCACTGGTTTTCAGGACGACCTTCAGGCCCAGCTCGTCCAGCAGGGTGAGGGTCAGTTGCGTCGCCTCCACCATGGCCTTCCAGGGGAGCGcagggtcc            >  2:27062/5‑149 (MQ=255)                                                                                                                                           ttgaCCCGGCGAGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTc                                                                            <  1:45201/82‑1 (MQ=255)                                                                                                                                           ttgaCCCGGCGAGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTc                                                                            >  2:45201/4‑85 (MQ=255)                                                                                                                                           cccACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGCTTAGCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCAGCGcagggtcag           <  1:496246/150‑1 (MQ=255)                                                                                                                                            ccACCCGGCTCGCCGTGGCAAGGGCACAACCAC‑GTGAATTCCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGTGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCAGCGcagggtcagg          <  2:317234/150‑1 (MQ=255)                                                                                                                                             cACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCAGCGcagggtcaggg         <  2:1519387/150‑1 (MQ=255)                                                                                                                                                ccGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCAGCGCAGGGTCAGGGTcc      >  2:1685256/1‑150 (MQ=255)                                                                                                                                                   gCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCAGCGCAGGGTCAGGGTCCAgg   <  1:490409/150‑1 (MQ=255)                                                                                                                                                    cTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCAGCGCAGGGTCAGGGTCCAGGt  <  1:870758/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      TAGCGCCCTTGCCGTTGCGCAGGTAGTCGATGAAAATGCGTCCCACGCGATTTTTCGGCCCCGACACCGCGCTCAGGCGCTCCGGAAACAGCTTGGCCAGGTAATTGACGATGGCGTGGCTGAAGTCCTTGACCTCGTCCCACCCGGCTCGCCGGGTCAAGGGCACAACCAC‑GTGAATACCCTTGCCACCACTGGTTTTCAGGAACACCTTCAGGCCCAGTTCGTCCAGCAGGGTGAGGGTCAGTTGGGTCGCCTCCACCATGGCCTTCCAGGGCAGCGCAGGGTCAGGGTCCAGGT  >  NC_002947/3693924‑3694220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑