Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,753,155 G→C 6.6% R257R (CGC→CGG)  PP_3316 ← chaperone‑associated ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,753,1550GC 6.6% 213.1 / 4.5 70R257R (CGC→CGG) PP_3316chaperone‑associated ATPase
Reads supporting (aligned to +/- strand):  ref base G (31/33);  new base C (2/3);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGTGCCCGCCCGATGACCGGGTCCAGCTTGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAACTCATTGGAGGCGACGAAGGCGCGACTGAGCG  >  NC_002947/3753007‑3753300
                                                                                                                                                    |                                                                                                                                                 
ccTGTGCCCGCCCGATGACCGGGTCCAGCTTGCCATCGCGCGCCATCCTGGTGAAATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGGCTCGGCGCGACCATCCTCTGCACACTTGGCAAGCACCTTGCTGACGCACTGgc                                                                                                                                                  >  1:910301/1‑150 (MQ=255)
    tgCCCGCCCGATGACCGGGTCCAGCTTGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAg                                                                                                                                              <  1:1445707/150‑1 (MQ=255)
      cccgcccgATGACCGGGTCCAGCTTGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAggg                                                                                                                                            >  1:5170/1‑150 (MQ=255)
       ccgcccgATGACCGGGTCCAGCTTGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGgc                                                                                                                                                  >  2:1711825/1‑143 (MQ=255)
       ccgcccgATGACCGGGTCCAGCTTGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGgc                                                                                                                                                  <  1:1711825/143‑1 (MQ=255)
       ccgcccgATGACCGGGTCCAGCTTGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGc                                                                                                                                           <  2:1673176/150‑1 (MQ=255)
         gcccgATGACCGGGTCCAGCTTGCCATCGGGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCt                                                                                                                                         >  1:52564/1‑150 (MQ=255)
         gcccgATGACCGGGTCCAGATTGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCAGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTATCCACGCTTGCCAACAACGTTGCTGACGCACTGACGCAGGGCCt                                                                                                                                         >  2:400335/1‑150 (MQ=255)
             gATGACCGGGTGCAGCTTACCATCGCGCGCCATCCTGGTGAGATCGCGCGGGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGGCCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCgg                                                                                                                                     <  2:1003818/150‑1 (MQ=255)
             gATGACCGGGTCCAGCTTGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCgg                                                                                                                                     <  2:1239395/150‑1 (MQ=255)
                     ggTCCAGCTTGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGc                                                                                                                             <  2:1034391/150‑1 (MQ=255)
                        ccAGCTTGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAgaagaa                                                                                                                          <  2:507582/150‑6 (MQ=255)
                        ccAGCTTGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCAt                                                                                                                          >  1:1035763/1‑150 (MQ=255)
                        ccAGCTTGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCAt                                                                                                                          >  1:944025/1‑150 (MQ=255)
                              tGCCATCGCGCGCCATCCTGGTGCGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGAGCAGGGCCTGCGGCATAAGGCCATAgcggc                                                                                                                    <  1:1442861/150‑1 (MQ=255)
                              tGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAgcggc                                                                                                                    <  2:800520/150‑1 (MQ=255)
                                 cATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCgca                                                                                                                 >  2:1672679/1‑150 (MQ=255)
                                     gcgcgcCATCCTGGTGAGATCGCGAGCGCTGTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCaa                                                                                                             >  1:50582/1‑150 (MQ=255)
                                         gcCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTg                                                                                                         <  1:1667579/150‑1 (MQ=255)
                                                 ggTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAg                                                                                                 >  2:1823843/1‑150 (MQ=255)
                                                    gagaTCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAgg                                                                                                                                             >  1:1203366/1‑103 (MQ=255)
                                                    gagaTCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAgg                                                                                                                                             <  2:1203366/103‑1 (MQ=255)
                                                       aTCGCTCCATTACTTGTCGAGCTCCGGCGTTTCGGGCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTc                                                                                           <  2:910301/150‑1 (MQ=255)
                                                       aTCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTc                                                                                           >  2:686771/1‑150 (MQ=255)
                                                       aTCGCGCGAGTACTTGGCGCGCTCCGGTGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTGGCCAACCACCTTGCTGACGCACTGCCGCAGGGCCTCCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTc                                                                                           <  1:1023872/150‑1 (MQ=255)
                                                        tCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCg                                                                                          <  2:714622/150‑1 (MQ=255)
                                                          gcgcgAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGCCGCAGGCCCTGCgccagt                                                                                                                                 <  2:942195/109‑6 (MQ=255)
                                                          gcgcgAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGCCGCAGGCCCTGCgccagt                                                                                                                                 >  1:942195/1‑104 (MQ=255)
                                                            gcgAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCct                                                                                      >  1:918612/1‑150 (MQ=255)
                                                             cgAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCctc                                                                                     >  2:1657810/1‑150 (MQ=255)
                                                                gTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTc                                                                                  <  1:1823843/150‑1 (MQ=255)
                                                                gTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTc                                                                                  <  1:370990/150‑1 (MQ=255)
                                                                 tACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCg                                                                                 <  1:249694/150‑1 (MQ=255)
                                                                    ttGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGCCGCAGGCCCTGCgc                                                                                                                                     <  1:435327/95‑2 (MQ=255)
                                                                    ttGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGCCGCAGGCCCTGCgc                                                                                                                                     >  2:435327/1‑94 (MQ=255)
                                                                      gTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGcc                                                                                                                            <  1:1651662/102‑1 (MQ=255)
                                                                      gTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGcc                                                                                                                            >  2:1651662/1‑102 (MQ=255)
                                                                        cGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGcc                                                                          <  2:211684/150‑1 (MQ=255)
                                                                        cGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGcc                                                                          >  1:345940/1‑150 (MQ=255)
                                                                              ccGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCa                                                                    <  2:711547/150‑1 (MQ=255)
                                                                               cGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCg                                                                                          >  2:749101/1‑127 (MQ=255)
                                                                               cGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCg                                                                                          <  1:749101/127‑1 (MQ=255)
                                                                                 gCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCaaga                                                                 >  2:1143512/1‑150 (MQ=255)
                                                                                    tttCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGGCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGt                                                              >  2:1500665/1‑150 (MQ=255)
                                                                                                 cTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTAc                                                 >  1:618391/1‑150 (MQ=255)
                                                                                                  tCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACg                                                >  2:1210885/1‑150 (MQ=255)
                                                                                                  tCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACg                                                >  2:218460/1‑150 (MQ=255)
                                                                                                  tCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACg                                                >  2:1028492/1‑150 (MQ=255)
                                                                                                     gcgcGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGAGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTAt                                             <  1:1672679/150‑1 (MQ=255)
                                                                                                       gcgACCATCCTCTGCAGCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGc                                           <  2:970974/150‑1 (MQ=255)
                                                                                                          aCCATCCTCTGCACCCTTGCCAACCACCGTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTg                                        >  2:995100/1‑150 (MQ=255)
                                                                                                           ccATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGc                                       <  2:918612/150‑1 (MQ=255)
                                                                                                            cATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGcc                                      >  1:1891672/1‑150 (MQ=255)
                                                                                                              tactctGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCgggg                                                      >  2:1833855/3‑132 (MQ=255)
                                                                                                              tactctGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCgggg                                                      <  1:1833855/130‑1 (MQ=255)
                                                                                                                 tctGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAAc                                 <  1:1271165/150‑1 (MQ=255)
                                                                                                                    gCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCgggg                                                      >  2:53448/1‑126 (MQ=255)
                                                                                                                    gCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCgggg                                                      <  1:53448/126‑1 (MQ=255)
                                                                                                                     cACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGACCCTACGTATGCGTGCCCCAACTCAt                             >  2:1581015/1‑150 (MQ=255)
                                                                                                                      aCCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCCGCGGCCGTGCCGCGGGGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAACTCAtt                            <  2:50582/150‑1 (MQ=255)
                                                                                                                         cTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAACTCATTGGa                         <  1:1210885/150‑1 (MQ=255)
                                                                                                                          ttGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAACTCATTGGAg                        <  1:1143512/150‑1 (MQ=255)
                                                                                                                          ttGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAACTCATTGGAg                        <  1:165773/150‑1 (MQ=255)
                                                                                                                            gCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATACCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAACTCATTGGAGGc                      <  2:52564/150‑1 (MQ=255)
                                                                                                                               aaCCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAACTCATTg                           >  1:148234/1‑142 (MQ=255)
                                                                                                                               aaCCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAACTCATTg                           <  2:148234/142‑1 (MQ=255)
                                                                                                                               aaCCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAACTCATTGGAGGcgac                   >  1:1598636/1‑150 (MQ=255)
                                                                                                                                accaccTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTc                                                          <  1:885869/110‑1 (MQ=255)
                                                                                                                                accaccTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTc                                                          >  2:885869/1‑110 (MQ=255)
                                                                                                                                     cTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCTCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAACTCATTGGAGGCGACGAAGgc             >  2:1386840/1‑150 (MQ=255)
                                                                                                                                         cTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTCGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAACTCATTGGAGGCGACGAAGGCGCGa         <  2:1035763/150‑1 (MQ=255)
                                                                                                                                          tGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCAACCGCGATCCAGAAGTGCTCGGGGCCTACGACTACGTGCTGGAGCTTTTTGGCATCGACGAAGGCGCGAc        >  1:974273/1‑150 (MQ=255)
                                                                                                                                                aCTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAACTCATTGGAGGCGACGAAGGCGCGACTGAgcg  >  2:635468/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCTGTGCCCGCCCGATGACCGGGTCCAGCTTGCCATCGCGCGCCATCCTGGTGAGATCGCGCGAGTACTTGTCGAGCTCCGGCGTTTCGGTCGGTGCCTCGGCGCGACCATCCTCTGCACCCTTGCCAACCACCTTGCTGACGCACTGGCGCAGGGCCTGCGGCATAAGGCCATAGCGGCGCAGCAAGTTGGCGGCCAGCCCTTCGCCCTCTTCGGCCAGCCCGATCAAGAAGTGCTCGGGGCCTACGTATGCGTGCCCCAACTCATTGGAGGCGACGAAGGCGCGACTGAGCG  >  NC_002947/3753007‑3753300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑