Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,791,147 G→T 8.2% G183V (GGC→GTC)  PP_3353 → sulfatase‑modifying protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,791,1470GT 8.2% 245.5 / 7.4 84G183V (GGC→GTC) PP_3353sulfatase‑modifying protein
Reads supporting (aligned to +/- strand):  ref base G (40/36);  new base T (2/5);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 2.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CGGACGTGCTCGGCCCCGGCTGGCAGTTCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTCAGTTCCCTTATCGCAATGCGGC  >  NC_002947/3791000‑3791294
                                                                                                                                                   |                                                                                                                                                   
cGGACGTGCTCGGCCCCGGCTGGCAGTTCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGcc                                                                                                                                                   <  1:1741247/150‑1 (MQ=255)
     gTGCTCGGCCCCGGCTGGCAGTTCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCCTCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGaa                                                                                                                                              <  1:129934/150‑1 (MQ=255)
            gCCCCGGCTGGCAGTTCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCa                                                                                                                                       >  1:1769444/1‑150 (MQ=255)
              cccGGCTGGCAGTTCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGTTCGCGGCCTGCCCAGc                                                                                                                                     <  1:1422856/150‑1 (MQ=255)
              cccGGCTGGCAGTTCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGc                                                                                                                                     >  2:1150273/1‑150 (MQ=255)
              cccGGCTGGCAGTTCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGc                                                                                                                                     <  1:1193720/150‑1 (MQ=255)
              cccGGCTGGCAGTTCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATAGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGTTCGCGGCCTGCCCAGc                                                                                                                                     <  2:149584/150‑1 (MQ=255)
              cccGGCGGGCAGTTCGGGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGTTCGCGGCCTGCCCAGc                                                                                                                                     <  2:919220/150‑1 (MQ=255)
               ccGGCTGGCAGTTCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGTTCGCCCTGGAAGATGCCCAGGCCGATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCg                                                                                                                                    >  1:1261529/1‑150 (MQ=255)
                cGGCTGGCAGTTCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGa                                                                                                                                   <  1:29435/150‑1 (MQ=255)
                    tGGCAGTTCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGgcg                                                                                                                               >  2:1878755/1‑150 (MQ=255)
                          ttCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAg                                                                                                                                  <  1:931824/141‑1 (MQ=255)
                          ttCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAg                                                                                                                                  >  2:931824/1‑141 (MQ=255)
                          ttCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTAGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTg                                                                                                                         <  2:502457/150‑1 (MQ=255)
                                 ccGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAgg                                                                                                                                 >  2:54916/1‑135 (MQ=255)
                                 ccGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAgg                                                                                                                                 <  1:54916/135‑1 (MQ=255)
                                            tGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCGATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAGTACGCCATGCgcggc                                                                                                       >  2:482405/1‑150 (MQ=255)
                                                   acCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGa                                                                                                                                   >  1:1924681/1‑115 (MQ=255)
                                                   acCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGa                                                                                                                                   <  2:1924681/115‑1 (MQ=255)
                                                    cccGCAAGGGCCGGGCAGCAGCCTGGGCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGAc                                                                                               <  1:238731/150‑1 (MQ=255)
                                                    cccGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGAc                                                                                               <  2:1233665/150‑1 (MQ=255)
                                                    cccGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGAc                                                                                               <  2:1261529/150‑1 (MQ=255)
                                                     ccGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGAcc                                                                                              >  2:76311/1‑150 (MQ=255)
                                                     ccGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGACCGCTGGGCACGATGACAACAACCCAACTGGTCGCAGCTGGAATACGCCATGCGCGGCGGCCTGAcc                                                                                              >  1:1376569/1‑150 (MQ=255)
                                                       gCAAGGGCCGGGCAGCAGCCTGGACGGTTGAGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGa                                                                                            >  1:989294/1‑150 (MQ=255)
                                                        tcaGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCAGCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCACCTGGAATACGCCATGCGCGGCGGCCTGACCGAt                                                                                           >  2:150652/3‑150 (MQ=255)
                                                             gCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGACGATGCCCAGGCCTATGCTCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCGTGCGCGGCGGCCTGACCGATGCCGa                                                                                      >  1:705933/1‑150 (MQ=255)
                                                                gggCAGCAGCCTGGCCGGGCTGGACAACCATCCGTTGTTGCAGATCGCCCTGTAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACtt                                                                                   <  2:1451380/150‑1 (MQ=255)
                                                                   cagcagCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAg                                                                                <  2:1065341/150‑1 (MQ=255)
                                                                         ccTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTgggg                                                                          >  1:316118/1‑150 (MQ=255)
                                                                           tGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCTTCTTGCCGCTGGGCAGACCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTa                                                                        >  1:233375/1‑150 (MQ=255)
                                                                           tGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTa                                                                        >  1:923086/1‑150 (MQ=255)
                                                                              ccGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTacca                                                                     <  1:1786077/150‑1 (MQ=255)
                                                                                  gCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGa                                                                 >  2:1651385/1‑150 (MQ=255)
                                                                                     ggACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGagca                                                              <  1:1892188/150‑1 (MQ=255)
                                                                                     ggACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGagca                                                              >  2:1266522/1‑150 (MQ=255)
                                                                                       aCAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTg                                                                             <  1:1353430/133‑1 (MQ=255)
                                                                                       aCAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTg                                                                             <  1:1353443/133‑1 (MQ=255)
                                                                                       aCAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTg                                                                             >  2:1353443/1‑133 (MQ=255)
                                                                                       aCAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTg                                                                             >  2:1353430/1‑133 (MQ=255)
                                                                                         aaCCATCCGGTGGTGCAGATCGCCCTGGAAGCTGCCCAGGCCTATGCCCGCTGGGCAGGCTGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGCTGCATACACAATCTCGGGGACCACCGGGCAGccc                                                          >  2:410264/1‑150 (MQ=255)
                                                                                         aaCCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGAAGCCTACATAAGCTGGGGGACCACCAAGCAGccc                                                          >  2:409942/1‑150 (MQ=255)
                                                                                          aCCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACt                                                                                                                                            <  1:1234295/67‑1 (MQ=255)
                                                                                          aCCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACt                                                                                                                                            >  2:1234295/1‑67 (MQ=255)
                                                                                           ccATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCaa                                                        >  1:910402/1‑150 (MQ=255)
                                                                                           ccATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGAGGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCaa                                                        <  1:279366/150‑1 (MQ=255)
                                                                                             aTCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCGGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTCCCACCGAGCAGCCCAAgg                                                      >  2:753190/1‑150 (MQ=255)
                                                                                             aTCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAgg                                                      <  1:637418/150‑1 (MQ=255)
                                                                                             aTCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAgg                                                      >  2:150497/1‑150 (MQ=255)
                                                                                             aTCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAgg                                                      >  2:1353864/1‑150 (MQ=255)
                                                                                             aTCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAgg                                                      <  1:614329/150‑1 (MQ=255)
                                                                                              tCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAggg                                                     <  1:1507205/150‑1 (MQ=255)
                                                                                                 ggtggtGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGTCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGAc                                                                                     <  2:358243/115‑1 (MQ=255)
                                                                                                 ggtggtGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGTCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGAc                                                                                     >  1:358243/1‑115 (MQ=255)
                                                                                                     gtgCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGACCTGCGCGGCCGCATGACCGATGCCGACTTCAGCTGGCCTGCCACCGAGCAGCCCAAGGGCAAGCTc                                              <  1:988599/150‑1 (MQ=255)
                                                                                                       gCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCAt                                            >  1:1046288/1‑150 (MQ=255)
                                                                                                        cAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATg                                           <  2:233375/150‑1 (MQ=255)
                                                                                                          gATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGc                                         <  1:1824531/150‑1 (MQ=255)
                                                                                                               cccTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATa                                    >  2:884517/1‑150 (MQ=255)
                                                                                                                ccTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATAc                                   <  2:338300/150‑1 (MQ=255)
                                                                                                                  tGGAAGATGCCGAGGACTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGCGCAGCCCAAGGGCAAGCTCATGGCCAATAccc                                 >  2:31102/1‑149 (MQ=255)
                                                                                                                  tGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCATTCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCt                                 >  1:329054/1‑150 (MQ=255)
                                                                                                                    gAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTAACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTgg                               >  1:973515/1‑150 (MQ=255)
                                                                                                                         tGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAAGACCTgatgaaa                          <  2:1419710/150‑7 (MQ=255)
                                                                                                                           cccAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTc                        <  1:689159/150‑1 (MQ=255)
                                                                                                                            ccAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCAt                                            <  1:1088429/129‑1 (MQ=255)
                                                                                                                            ccAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCAt                                            >  2:1088429/1‑129 (MQ=255)
                                                                                                                            ccAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTCa                       >  2:995074/1‑150 (MQ=255)
                                                                                                                            ccAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTCa                       <  2:1376569/150‑1 (MQ=255)
                                                                                                                              aGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGGTGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCt                                 <  1:380403/138‑1 (MQ=255)
                                                                                                                              aGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCt                                 >  2:380403/1‑138 (MQ=255)
                                                                                                                                 ccTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTCAGTTcc                  <  2:1445634/150‑1 (MQ=255)
                                                                                                                                 ccTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTCAGTTcc                  <  2:910402/150‑1 (MQ=255)
                                                                                                                                   tATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTCAGTTCCCt                >  2:296122/1‑150 (MQ=255)
                                                                                                                                     tGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTCAGTTCCCTTa              <  1:127915/150‑1 (MQ=255)
                                                                                                                                      cctcGCTGGGCAGTTCGCGGCCTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGcc                                                                                        >  1:1261090/4‑75 (MQ=21)
                                                                                                                                      cctcGCTGGGCAGTTCGCGGCCTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGcc                                                                                        <  2:1261090/72‑1 (MQ=25)
                                                                                                                                       cccGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATCCGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTCAGTTCCCTTATc            <  1:1532018/150‑1 (MQ=255)
                                                                                                                                       cccGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTCAGTTCCCTTATc            <  2:923086/150‑1 (MQ=255)
                                                                                                                                       cccGCTGGGCAGGCCGCGAACTGCCAAGCGAGGCGCCGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGGAGGGTCAGATCCCTTATc            >  2:393789/1‑150 (MQ=255)
                                                                                                                                           cTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTCAGTTCCCTTATCGCaa        >  1:1731509/1‑150 (MQ=255)
                                                                                                                                            tGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTCAGTTCCCTTATCGCAAt       >  1:810988/1‑150 (MQ=255)
                                                                                                                                                 aGGCCGCGAACTGCCCAGCGGGGCGCAGCTGGAATACGCCATGTGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTAAGTTCCCTTATCGCAATgcggc  >  1:439673/1‑150 (MQ=255)
                                                                                                                                                 aGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTCAGTTCCCTTATCGCAATgcggc  >  2:271615/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGGACGTGCTCGGCCCCGGCTGGCAGTTCGTGCCCGGCGCCAACTGGCGACACCCGCAAGGGCCGGGCAGCAGCCTGGCCGGGCTGGACAACCATCCGGTGGTGCAGATCGCCCTGGAAGATGCCCAGGCCTATGCCCGCTGGGCAGGCCGCGAACTGCCCAGCGAGGCGCAGCTGGAATACGCCATGCGCGGCGGCCTGACCGATGCCGACTTCAGCTGGGGTACCACCGAGCAGCCCAAGGGCAAGCTCATGGCCAATACCTGGCAGGGTCAGTTCCCTTATCGCAATGCGGC  >  NC_002947/3791000‑3791294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑