Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,797,549	A→T	7.8%	L163Q (CTG→CAG)	vdh ←	vanillin dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,797,549	0	A	T	7.8%	190.0 / 7.0	64	L163Q (CTG→CAG)	vdh	vanillin dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (32/27);  new base T (3/2);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01

CGATGGCGGGGGCATCCTGCGGCGCATTGCTGATGACATTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAGGGGATCACTTCACCGGTGATCTGCGTG  >  NC_002947/3797403‑3797692                                                                                                                                                   |                                                                                                                                                cgaTGGCGGGGGCATCCTGCGGCGCATTGCTGATGACATTGACCACGATCAGGCTGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCg                                                                                                                                              <  1:1553092/150‑1 (MQ=255)  gaTGGCGGGGGCATCCTGCGGCGCATTGCTGATGACATTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCgg                                                                                                                                             >  2:684204/1‑150 (MQ=255)    tGGCGGGGGCATCCTGCGGCGCATTGCTGATGACATTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGTTCCACGGTGTTGCCTCAGGCCAGCGGCa                                                                                                                                           <  2:218150/150‑1 (MQ=255)     ggcgggcgCATCCTGCGGCGCATTGCTGATGACATTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCTGGCCTGCGGCaa                                                                                                                                          <  1:771813/150‑2 (MQ=255)     ggCGGGGGCATCCTGCGGCGCATTGCTGATGACATTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCat                                                                                                                                          <  1:942711/150‑1 (MQ=255)                      gcgcATTGCTGATGACATTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTgg                                                                                                                         <  1:344117/150‑1 (MQ=255)                             gCTGATGACATTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTAt                                                                                                                  <  2:1443277/150‑1 (MQ=255)                               tgatgaCATTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCa                                                                                                                >  2:1863415/1‑150 (MQ=255)                                gatgaCATTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCAc                                                                                                               >  1:998062/1‑150 (MQ=255)                                    ccATTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCggg                                                                                                           <  2:1238701/149‑1 (MQ=255)                                    acacTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCggg                                                                                                           <  1:1006833/150‑1 (MQ=255)                                    aCATTGACCACGCCGTCGCCGATGCCTCCATCGTGCCGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCggg                                                                                                           <  2:1842783/150‑1 (MQ=255)                                     cATTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCgggg                                                                                                          >  2:1501833/1‑150 (MQ=255)                                     cATTGACCACGCCGTCGCCGATGCCGGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCgggg                                                                                                          <  2:606219/150‑1 (MQ=255)                                      aTTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGc                                                                                                         >  2:420916/1‑150 (MQ=255)                                      aTTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGc                                                                                                         >  2:430401/1‑150 (MQ=255)                                      aTTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGGGGCCAGTATCACCggggg                                                                                                         >  2:219010/1‑149 (MQ=255)                                       ttGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCg                                                                                                        >  1:596232/1‑150 (MQ=255)                                          cccACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTc                                                                                                     <  2:1434910/149‑1 (MQ=255)                                            cACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCa                                                                                                   >  2:622024/1‑150 (MQ=255)                                              cGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACg                                                                                                 <  1:1501833/150‑1 (MQ=255)                                                  gTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCa                                                                                                                      >  1:237207/1‑125 (MQ=255)                                                  gTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCa                                                                                                                      <  2:237207/125‑1 (MQ=255)                                                              tGCNTCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAacac                                                                                 >  2:1201350/1‑150 (MQ=255)                                                                 aTCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAAcaccac                                                                              <  1:236295/150‑1 (MQ=255)                                                                        agcaCCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAg                                                                       <  1:1640615/150‑1 (MQ=255)                                                                         gcaCCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAgg                                                                      >  2:1621329/1‑150 (MQ=255)                                                                         gcaCCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAgg                                                                      >  1:899559/1‑150 (MQ=255)                                                                           aCCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGgc                                                                    <  1:684204/150‑1 (MQ=255)                                                                           aCCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCAATGCCCAACACCACGCCGCAGGgc                                                                    <  2:596232/150‑1 (MQ=255)                                                                            ccTAGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGgcg                                                                   >  1:1073483/1‑150 (MQ=255)                                                                                gCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGCTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGgcgcgcg                                                               >  2:800511/1‑150 (MQ=255)                                                                                     tCAGCCGATGGACCGCCGCGCTCAGCTCGGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAggg                                                          <  1:1369509/150‑1 (MQ=255)                                                                                      cAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCATGGc                                                         <  2:659128/150‑1 (MQ=255)                                                                                          cGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCAtt                                                     <  1:622024/150‑1 (MQ=255)                                                                                               gACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGCTGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGaa                                                >  1:257291/1‑150 (MQ=255)                                                                                               gACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGaa                                                >  2:423671/1‑150 (MQ=255)                                                                                                 ccgccgGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGc                                              >  1:438622/1‑150 (MQ=255)                                                                                                  cgccgGGGTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCt                                             >  2:118781/1‑150 (MQ=255)                                                                                                  cgccgGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCt                                             >  2:1788903/1‑150 (MQ=255)                                                                                                  cgccgGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCt                                             >  2:1415298/1‑150 (MQ=255)                                                                                                   gccgGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTg                                            >  2:86737/1‑150 (MQ=255)                                                                                                    ccgGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCCGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGc                                           >  1:364257/1‑150 (MQ=255)                                                                                                         cTCAGCGCCGAGGCCTTGAGCACCACGGTGTGGCCGCAGGCCAGCGGCATGGAAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGGGCGATGCCCAACACCCCGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGa                                      <  2:343200/150‑1 (MQ=255)                                                                                                            aGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTg                                                    <  2:1729139/133‑1 (MQ=255)                                                                                                            aGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTg                                                    >  1:1729139/1‑133 (MQ=255)                                                                                                              cTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTc                                 >  2:1885789/1‑150 (MQ=255)                                                                                                                 cGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGATACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGa                              >  1:1788997/1‑150 (MQ=255)                                                                                                                  gAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAg                             >  2:1103405/1‑150 (MQ=255)                                                                                                                  gAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAg                             <  1:430401/150‑1 (MQ=255)                                                                                                                        ttGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAGGGGATc                       >  1:1492298/1‑150 (MQ=255)                                                                                                                          gAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAGGGGATCAc                     <  1:544627/150‑1 (MQ=255)                                                                                                                                cacGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGGTGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAGGGGGTCACTTCAcc               >  1:1180757/1‑150 (MQ=255)                                                                                                                                cacGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAAc                                    <  2:1442536/129‑1 (MQ=255)                                                                                                                                cacGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAAc                                    >  1:1442536/1‑129 (MQ=255)                                                                                                                                      catGCCGCTGGCCTGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCAtt                                                     >  2:1028954/3‑106 (MQ=255)                                                                                                                                      catGCCGCTGGCCTGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCAtt                                                     <  1:1028954/104‑1 (MQ=255)                                                                                                                                      catGCCGCTGGCCTGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAGGGGATCACTTCACCGGTGAt         >  1:458345/3‑150 (MQ=255)                                                                                                                                       ttGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAGGGGATCACTTCACCGGTGATc        <  2:708296/150‑1 (MQ=255)                                                                                                                                       atGCCGCTGGCCTGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAGGGGATCACTTCACCGGTGATc        >  2:501411/2‑150 (MQ=255)                                                                                                                                          ccGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAGGGGATCACTTCACCGGTGATCTGc     <  1:1243580/150‑1 (MQ=255)                                                                                                                                          ccGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAGGGGATCACTTCACCGGTGATCTGc     >  1:1102483/1‑150 (MQ=255)                                                                                                                                           cGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAGGGGATCACTTCACCGGTGATCTGCg    >  2:1700712/1‑150 (MQ=255)                                                                                                                                             cAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAGGGGATCACTTCACCGGTGATCTGCGTg  <  1:1621329/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                CGATGGCGGGGGCATCCTGCGGCGCATTGCTGATGACATTGACCACGCCGTCGCCGATGCCTGCATCGTGCAGCACCTGGCCGATCAGCCGATGGACCGCCGGGCTCAGCTCCGAGGCCTTGAGCACCACGGTGTTGCCGCAGGCCAGCGGCATGGCAATGGCACGCGTGGCCAGTATCACCGGGGCGTTCCACGGTGCGATGCCCAACACCACGCCGCAGGGCGCGCGCAGGGCCATTGCGAAGCTGCCGGGAACGTCCGAGGGGATCACTTCACCGGTGATCTGCGTG  >  NC_002947/3797403‑3797692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑