Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 296,372 T→G 5.9% E19A (GAG→GCG) ‡ PP_0242 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947296,3720TG 5.9% 236.9 / 5.3 84E19A (GAG→GCG) ‡PP_0242TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (38/41);  new base G (2/3);  total (40/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

AGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGCCTGGCG  >  NC_002947/296228‑296520
                                                                                                                                                |                                                                                                                                                    
aGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCt                                                                                                                                                      <  1:1808782/145‑1 (MQ=255)
aGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCt                                                                                                                                                      >  2:1808782/1‑145 (MQ=255)
aGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATc                                                                                                                                                 <  2:1631578/150‑1 (MQ=255)
   gCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctg                                                                                                                                              <  1:1271816/150‑1 (MQ=255)
    ccTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctgg                                                                                                                                             >  1:1579905/1‑150 (MQ=255)
     cTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGAGGCGATcctggc                                                                                                                                            <  2:173476/150‑1 (MQ=255)
     cTCCAGCAAGGCCGGCACGCTGGCGAAAAAGCGATCGACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggc                                                                                                                                            <  2:449107/150‑1 (MQ=255)
      tCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggcc                                                                                                                                           >  2:1398975/1‑150 (MQ=255)
        cAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggcctg                                                                                                                                         >  1:1067428/1‑150 (MQ=255)
        cAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggcctg                                                                                                                                         >  2:638292/1‑150 (MQ=255)
         aGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCTAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggcctgg                                                                                                                                        >  1:1604518/1‑150 (MQ=255)
         aGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggcctgg                                                                                                                                        <  2:1718060/150‑1 (MQ=255)
         aGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGAGGCGATcctgg                                                                                                                                             <  2:1915105/145‑1 (MQ=255)
         aGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGAGGCGATcctgg                                                                                                                                             >  1:1915105/1‑145 (MQ=255)
                      cGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTg                                                                                                                           <  2:592240/150‑1 (MQ=255)
                       gCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTgg                                                                                                                          <  2:1775651/150‑1 (MQ=255)
                       gCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTgg                                                                                                                          >  1:1862497/1‑150 (MQ=255)
                       gCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTgg                                                                                                                          >  1:886886/1‑150 (MQ=255)
                         tGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGgcgc                                                                                                                              <  1:106206/144‑1 (MQ=255)
                         tGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGgcgc                                                                                                                              >  2:106206/1‑144 (MQ=255)
                         tGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGtt                                                                                                                        >  2:1713380/1‑150 (MQ=255)
                             gAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTgcg                                                                                                                    >  2:1925754/1‑150 (MQ=255)
                                aaaGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCCCCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCgg                                                                                                                 <  1:1398975/150‑1 (MQ=255)
                                    tGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtg                                                                                                             <  1:130921/150‑1 (MQ=255)
                                    tGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCAATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtg                                                                                                             >  2:1117967/1‑150 (MQ=255)
                                      aTACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggt                                                                                                           <  1:416464/150‑1 (MQ=255)
                                          acCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGgtggtggtg                                                                                                       >  1:821751/1‑150 (MQ=255)
                                           ccGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGc                                                                                                      <  1:1293907/150‑1 (MQ=255)
                                            cgacgaAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCg                                                                                                     >  2:570210/1‑150 (MQ=255)
                                            cgacgaAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGACACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCg                                                                                                     <  2:100282/150‑1 (MQ=255)
                                                     cGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCaa                                                                                            >  2:271472/1‑150 (MQ=255)
                                                      ggAATCTGCGCCCGCTCGGCCACGCAGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAAc                                                                                           >  2:641451/1‑150 (MQ=255)
                                                          tCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggtg                                                                                                          <  2:1295321/131‑1 (MQ=255)
                                                          tCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggtg                                                                                                          >  1:1295321/1‑131 (MQ=255)
                                                            tGCGCGCGCTCGGCCAGGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTGGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGa                                                                                     <  2:595000/150‑1 (MQ=255)
                                                                cccGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGc                                                                                 >  1:1656162/1‑150 (MQ=255)
                                                                 ccGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGt                                                                                                   <  1:569792/131‑1 (MQ=255)
                                                                 ccGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGt                                                                                                   >  2:569792/1‑131 (MQ=255)
                                                                  cGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCa                                                                               <  2:270506/150‑1 (MQ=255)
                                                                   gCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAg                                                                              <  2:1862497/150‑1 (MQ=255)
                                                                   gCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAg                                                                              <  2:886886/150‑1 (MQ=255)
                                                                   gCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAg                                                                              <  2:1533182/150‑1 (MQ=255)
                                                                          cACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGAtt                                                                                   >  2:1790900/1‑138 (MQ=255)
                                                                          cACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGAtt                                                                                   <  1:1790900/138‑1 (MQ=255)
                                                                               tGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTg                                                                  <  2:683630/150‑1 (MQ=255)
                                                                                gTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTgg                                                                 >  1:1284972/1‑150 (MQ=255)
                                                                                  agatagaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTTGTa                                                               <  1:570210/150‑1 (MQ=255)
                                                                                       gaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGNTGCGGTNCATCAACTGCTGNTTGCCAGGATGCTGGCCTGGTAGgngc                                                          <  1:1925754/150‑1 (MQ=255)
                                                                                        aaaGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGcc                                                         >  1:591890/1‑150 (MQ=255)
                                                                                        aaaGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGcc                                                         <  2:855784/150‑1 (MQ=255)
                                                                                           gTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCcgac                                                      >  1:777680/1‑150 (MQ=255)
                                                                                              ctgcCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGaa                                                   <  1:1639061/150‑1 (MQ=255)
                                                                                              ctgcCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGaa                                                   >  2:4603/1‑150 (MQ=255)
                                                                                                 ccACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAg                                                >  2:326411/1‑150 (MQ=255)
                                                                                                   aCCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGttt                                                                                                                       <  1:1842678/77‑1 (MQ=255)
                                                                                                   aCCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGttt                                                                                                                       >  2:1842678/1‑77 (MQ=255)
                                                                                                   aCCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGTTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGt                                              >  2:520123/1‑150 (MQ=255)
                                                                                                    ccccTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGTCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTc                                             >  1:1184840/1‑150 (MQ=255)
                                                                                                    ccccTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCg                                                                                                     <  1:898499/94‑1 (MQ=255)
                                                                                                    ccccTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCg                                                                                                     >  2:898499/1‑94 (MQ=255)
                                                                                                    ccccTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTc                                             <  1:1713380/150‑1 (MQ=255)
                                                                                                       cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGaa                                          >  2:1760045/1‑150 (MQ=255)
                                                                                                       cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGaa                                          >  1:1123482/1‑150 (MQ=255)
                                                                                                       cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGaa                                          <  2:1284972/150‑1 (MQ=255)
                                                                                                           ttCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCa                                      <  1:404519/150‑1 (MQ=255)
                                                                                                                ccAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGATAGGCGCCGACGAACAGGTCGAAGTCACCGAc                                 <  2:1067428/150‑1 (MQ=255)
                                                                                                                   aCAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTc                              <  1:638292/150‑1 (MQ=255)
                                                                                                                      gCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGGGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGtt                           >  1:980397/1‑150 (MQ=255)
                                                                                                                        tCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCg                                                                                                     >  1:280938/1‑74 (MQ=255)
                                                                                                                        tCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCg                                                                                                     <  2:280938/74‑1 (MQ=255)
                                                                                                                            gcgcAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTtcat                                                                                               >  2:901166/1‑76 (MQ=255)
                                                                                                                            gcgcAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTtcat                                                                                               <  1:901166/76‑1 (MQ=255)
                                                                                                                                   cgccAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCg              <  1:1186545/150‑1 (MQ=255)
                                                                                                                                    gccAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACCTCGTTTTGCTCCAGCTctg             >  2:410750/1‑148 (MQ=255)
                                                                                                                                    gccAGGATCGAGGCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGaa                                          >  2:403190/1‑121 (MQ=255)
                                                                                                                                    gccAGGATCGAGGCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGaa                                          <  1:403190/121‑1 (MQ=255)
                                                                                                                                     ccAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGttt                          <  2:856988/136‑1 (MQ=255)
                                                                                                                                     ccAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGttt                          >  1:856988/1‑136 (MQ=255)
                                                                                                                                      cAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGAc           <  2:591890/150‑1 (MQ=255)
                                                                                                                                         gATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGc        <  1:1231482/150‑1 (MQ=255)
                                                                                                                                              ccTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGc                       <  1:1804669/130‑1 (MQ=255)
                                                                                                                                              ccTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGc                       >  2:1804669/1‑130 (MQ=255)
                                                                                                                                              ccTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTCCTCCAGCTCGGACTGCCTGgc   <  2:1459889/150‑1 (MQ=255)
                                                                                                                                              ccTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGACGACGTGCGCGTCGTGGTCGCCTTCTTCGTTTTGCTCCAGCTCGGACTGCCTGgc   <  2:1656162/150‑1 (MQ=255)
                                                                                                                                               cTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGCACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGCCTGgcg  >  2:410033/1‑150 (MQ=255)
                                                                                                                                               cTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGACGTCGCCGACTTCGTTTTGCTCCAGCTCGGACTGCCTGgcg  >  1:864343/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
AGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGCCTGGCG  >  NC_002947/296228‑296520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑