| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,984,085 | C→T | 6.8% | intergenic (+35/+155) | ilvE → / ← PP_3512 | branched‑chain‑amino acid aminotransferase/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,984,085 | 0 | C | T | 6.8% | 170.6 / 2.7 | 59 | intergenic (+35/+155) | ilvE/PP_3512 | branched‑chain‑amino acid aminotransferase/membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (33/22); new base T (2/2); total (35/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
CCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCGGGTG > NC_002947/3983939‑3984233 | ccACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAAt > 2:679338/1‑150 (MQ=255) cACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATg > 1:1099704/1‑150 (MQ=255) cTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGc > 1:540153/1‑150 (MQ=255) tGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGcc > 1:791093/1‑150 (MQ=255) aaaaGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCg < 2:1536999/150‑1 (MQ=255) cGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTc < 2:791093/150‑1 (MQ=255) ggCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCa > 1:1704461/1‑150 (MQ=255) gCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCAt < 1:1128117/150‑1 (MQ=255) cccGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCAtg < 2:180017/150‑1 (MQ=255) cccGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCAtg > 2:1437635/1‑150 (MQ=255) ccAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTacac > 1:149883/1‑150 (MQ=255) ccAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTacac < 1:710055/150‑1 (MQ=255) aaCGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAg > 1:823060/1‑150 (MQ=255) gAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGTAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAg > 2:1915286/1‑150 (MQ=255) gAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAg < 1:679338/150‑1 (MQ=255) aGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGc < 1:461872/150‑1 (MQ=255) gCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATgg < 1:923669/104‑1 (MQ=255) gCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATgg > 2:923669/1‑104 (MQ=255) cTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCgg < 2:1704461/150‑1 (MQ=255) cttgacgaTCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGt > 2:900893/8‑150 (MQ=255) aCCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTGTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGtt > 1:1563950/1‑150 (MQ=255) gTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGACCGTCAAGGGCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCAGGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTAcc > 2:1377688/1‑150 (MQ=255) ggcgATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGc < 1:900893/150‑1 (MQ=255) gagGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCgc > 2:420894/1‑150 (MQ=255) ggCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCgcca < 2:836362/150‑1 (MQ=255) aGGCTGGATCGTCAAGGTCGCCTGAAACGCAACAGCTTTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTGGTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGCTTACCCGCGAATAGGCAACACCCGCCAGCCaataat > 1:1094982/1‑150 (MQ=255) gCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCgg > 2:382014/1‑110 (MQ=255) gCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCgg < 1:382014/110‑1 (MQ=255) gATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTgg < 2:1563950/150‑1 (MQ=255) tCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGCGCCGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATACTGCCTgggg > 1:861444/1‑149 (MQ=255) gTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGgaga > 1:1282091/1‑150 (MQ=255) tCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGaa > 2:1846241/1‑114 (MQ=255) tCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGaa < 1:1846241/114‑1 (MQ=255) tCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGAt > 1:1696083/1‑150 (MQ=255) gTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCg > 2:1724124/1‑150 (MQ=255) gCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCAcc > 1:1018819/1‑146 (MQ=255) gCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCAcc < 2:1018819/146‑1 (MQ=255) cTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGCATAAGATCCTGCCGCCATCCAAGAATGCCTGGGAGATCACCGTTGTc > 1:1374407/1‑150 (MQ=255) tGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCa > 1:1264746/1‑150 (MQ=255) gAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGc < 2:732536/107‑1 (MQ=255) gAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGc > 1:732536/1‑107 (MQ=255) aCGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGc > 1:1353647/1‑150 (MQ=255) gCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGccccc < 2:1114626/72‑1 (MQ=255) gCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGccccc > 1:1114626/1‑72 (MQ=255) tCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATACCTGGGAGCTCACCGTTGTCAGTGCAGccc > 2:391167/1‑149 (MQ=255) gCTGTATGAAAACGGGGCATGCCAGTAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCAt < 1:1915286/150‑1 (MQ=255) tATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGc > 2:1182684/1‑150 (MQ=255) tATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGc > 2:590524/1‑150 (MQ=255) aTGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCg < 1:1437635/150‑1 (MQ=255) tGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCgg < 2:149883/150‑1 (MQ=255) tGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCgg < 1:590524/150‑1 (MQ=255) aaCGGGGCATGCCATTGCTGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAg > 1:372877/1‑84 (MQ=25) aaCGGGGCATGCCATTGCTGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAg < 2:372877/84‑1 (MQ=25) gggCATGCCAGCAATGGCATGTCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGccc < 1:916547/150‑1 (MQ=255) ggCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCcg > 2:1240153/1‑150 (MQ=255) gCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCcgc > 1:395137/1‑150 (MQ=255) cAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACATGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCgg < 1:967626/134‑1 (MQ=255) cAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACATGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCgg > 2:967626/1‑134 (MQ=255) gCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCGGGTg > 2:73881/1‑150 (MQ=255) | CCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCGGGTG > NC_002947/3983939‑3984233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |