Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,049,951 A→G 22.6% intergenic (+40/‑61) quiA → / → oprB‑III quinate dehydrogenase/carbohydrate‑selective porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,049,9510AG22.6% 54.6 / 14.6 36intergenic (+40/‑61)quiA/oprB‑IIIquinate dehydrogenase/carbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base A (12/15);  new base G (3/5);  total (16/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

TGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAG  >  NC_002947/4049802‑4050100
                                                                                                                                                     |                                                                                                                                                      
tGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACaa                                                                                                                                                        <  1:447438/150‑1 (MQ=255)
      aCGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGgccc                                                                                                                                                  <  2:222090/150‑1 (MQ=255)
      aCGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGACAGCCCGCGCCTACCCGGcccc                                                                                                                                                  >  2:1544098/1‑150 (MQ=255)
           tcGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACaa                                                                                                                                                        >  1:1478319/1‑139 (MQ=255)
           tcGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACaa                                                                                                                                                        <  2:1478319/139‑1 (MQ=255)
           tcGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAg                                                                                                                                                       >  2:1497809/1‑140 (MQ=255)
           tcGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAg                                                                                                                                                       <  1:1497809/140‑1 (MQ=255)
             gCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGa                                                                                                                                           <  2:1549927/150‑1 (MQ=255)
             gCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGa                                                                                                                                           <  2:1468228/150‑1 (MQ=255)
              ccGAAGACCGCCGAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGGCGGTCGACTGACCGGGGCGGCTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGAg                                                                                                                                          <  2:534783/150‑1 (MQ=38)
               cGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGc                                                                                                                                         <  2:1749990/150‑1 (MQ=255)
                 aaGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCgg                                                                                                                                       <  2:1255889/150‑1 (MQ=255)
                 aaGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCgg                                                                                                                                       <  2:1227034/150‑1 (MQ=255)
                 aaGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGAATATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCgg                                                                                                                                       <  2:1591194/150‑1 (MQ=255)
                 aaGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCCGCAGTCTACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCgg                                                                                                                                       <  1:409109/150‑1 (MQ=255)
                  aGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGAGCggg                                                                                                                                      <  2:581610/150‑1 (MQ=255)
                  aGACCGGCAAGCAGTACGTGGTGATCAATGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGCCCCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCggg                                                                                                                                      <  2:1011196/150‑1 (MQ=255)
                   gACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGc                                                                                                                                     >  1:1202510/1‑150 (MQ=255)
                                                     ggcgCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGAg                                                                                                                                          <  2:248760/111‑1 (MQ=38)
                                                     ggcgCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGAg                                                                                                                                          >  1:248760/1‑111 (MQ=38)
                                                                gTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGc                                                                                                                                                     <  1:788723/89‑1 (MQ=255)
                                                                gTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGc                                                                                                                                                     >  2:788723/1‑89 (MQ=255)
                                                                                               tACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCctgct                                                        <  2:624594/150‑1 (MQ=255)
                                                                                                 cGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCGCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCctgctgc                                                      <  1:1544098/150‑1 (MQ=255)
                                                                                                                  gcgTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGcacc                                     >  1:1344283/1‑150 (MQ=255)
                                                                                                                                  gggCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGcg                     >  1:1796342/1‑150 (MQ=255)
                                                                                                                                      aaGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGcgacga                 <  1:1098570/150‑1 (MQ=255)
                                                                                                                                         cccGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCCTCAAAGCCAACCCGAGATTCTGTAATGCCATCCGCAATTCCCATCACCCCCACCCTGCTGCTGGCCCTGGCAACCACCACTCCCCTTCCCAGcgacgacc               >  2:1746384/1‑148 (MQ=255)
                                                                                                                                          ccGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGAtctc             >  2:1628198/1‑150 (MQ=255)
                                                                                                                                            gCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAAcc                                                                                                       >  1:1836899/1‑58 (MQ=255)
                                                                                                                                            gCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAAcc                                                                                                       <  2:1836899/58‑1 (MQ=255)
                                                                                                                                            gCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGCTCTCAt           >  2:94539/1‑150 (MQ=255)
                                                                                                                                            gCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCAt           >  1:522202/1‑150 (MQ=255)
                                                                                                                                            gCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCAAGCGACGATCTCag           >  2:110508/1‑149 (MQ=255)
                                                                                                                                             cTCCTACAGGGGCCTTGTAGGAGCGGGCTTG‑CCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGa         >  1:483871/1‑150 (MQ=255)
                                                                                                                                                cTACAGGGGCCTTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGAcc       >  2:372512/1‑150 (MQ=255)
                                                                                                                                                cTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGctggccag                                               <  2:287184/110‑3 (MQ=255)
                                                                                                                                                cTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGctggccag                                               >  1:287184/1‑108 (MQ=255)
                                                                                                                                                  aCAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCg     <  2:1344283/150‑1 (MQ=255)
                                                                                                                                                     aGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCgcag  <  1:94539/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATG‑CCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAG  >  NC_002947/4049802‑4050100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑