Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,082,785 T→G 11.6% L229R (CTG→CGG) ‡ PP_3592 → RpiR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,082,7850TG11.6% 131.6 / 11.2 58L229R (CTG→CGG) ‡PP_3592RpiR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (22/29);  new base G (2/5);  total (24/34)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.22e-01

CTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATGGTCAACGAGGTCTGCAAGAAGCTCGGA  >  NC_002947/4082637‑4082933
                                                                                                                                                    |                                                                                                                                                    
cTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTg                                                                                                                                                     <  2:28218/150‑1 (MQ=255)
  ggTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGt                                                                                                                                                   <  1:839746/150‑1 (MQ=255)
   gTCTACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTg                                                                                                                                                  >  2:473815/1‑150 (MQ=255)
      gACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCAGGGTGAcc                                                                                                                                               <  1:297217/150‑1 (MQ=255)
           cAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCgcaat                                                                                                                                          <  1:381366/150‑5 (MQ=255)
           cAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCAGGGTGACCgcaat                                                                                                                                          <  2:1901042/150‑5 (MQ=255)
              ccagcagCACTTCGCCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCtt                                                                                                                                       >  2:1681190/7‑150 (MQ=255)
               gcCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTCCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTc                                                                                                                                      <  2:920456/150‑1 (MQ=255)
                ccGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCt                                                                                                                                     >  1:828042/1‑150 (MQ=255)
                  cagCACTTCGCCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCtgt                                                                                                                                   >  1:674733/3‑150 (MQ=255)
                     cACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGAc                                                                                                                                >  1:132775/1‑150 (MQ=255)
                        ttCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTgg                                                                                                                             >  1:1875096/1‑150 (MQ=255)
                        ttCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTgg                                                                                                                             <  1:67066/150‑1 (MQ=255)
                              gAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCCCCCTGGTGACCGACACCTTCTGTGACTGGGCGGAt                                                                                                                       <  2:619089/150‑1 (MQ=255)
                              gAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGAt                                                                                                                       <  1:1756794/150‑1 (MQ=255)
                              gAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGAt                                                                                                                       <  2:619108/150‑1 (MQ=255)
                              gAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGAt                                                                                                                       <  1:694318/150‑1 (MQ=255)
                                    ctgctgGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAAt                                                                                                                 <  1:473815/150‑1 (MQ=255)
                                    ctgctgGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAAt                                                                                                                 <  1:805894/150‑1 (MQ=255)
                                     tgctgGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATg                                                                                                                <  2:1177069/150‑1 (MQ=255)
                                     tgctgGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATg                                                                                                                >  1:1294491/1‑150 (MQ=255)
                                                 ccGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGgtgt                                                                                                    >  2:283522/1‑150 (MQ=255)
                                                   gAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATCGCGGTCACCCTGCTGACCGACACCTTCTGTGACTGGGCGGATACCAATGCCGACGAGGTGTTc                                                                                                  <  2:1055016/150‑1 (MQ=255)
                                                         aGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCATGATGACCGACACCTCCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATc                                                                                            <  2:1417978/150‑1 (MQ=255)
                                                                  gtggtgTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAg                                                                                   <  2:1412878/150‑1 (MQ=255)
                                                                      tgtTCTAGGCGCGCCGTTATTCCCGCCATGACCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCGCCCTGGTGACCGACACCTTCTGTGACTGGGCGGGTGCCAATGCCGACGAGGGGATCCGCACCCCCACCAAGCTCa                                                                               >  2:809321/1‑150 (MQ=255)
                                                                      tgtTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATc                                                                                            >  1:926710/1‑137 (MQ=255)
                                                                      tgtTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATc                                                                                            <  2:926710/137‑1 (MQ=255)
                                                                        ttCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGTTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAAc                                                                             >  1:1381310/1‑150 (MQ=255)
                                                                               cgcgcCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCt                                                                      <  2:1875096/150‑1 (MQ=255)
                                                                                gcgcCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGCGCTCAACCTGTtgtg                                                                     >  2:1151165/1‑150 (MQ=255)
                                                                                 cgcCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTgg                                                                    <  2:600512/150‑1 (MQ=255)
                                                                                  gcCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCAGGGTGACCgcaa                                                                                                                                           <  2:998621/78‑4 (MQ=39)
                                                                                  gcCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCAGGGTGACCgcaa                                                                                                                                           >  1:998621/1‑75 (MQ=39)
                                                                                    cGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGa                                                                                    >  1:1045714/1‑131 (MQ=255)
                                                                                    cGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGa                                                                                    <  2:1045714/131‑1 (MQ=255)
                                                                                     gTTAGTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGAACGACACATTCTGTGACTGGGCGGATGCCAATGCCGCCGAGGTGTGCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAAt                                                                >  2:888664/1‑150 (MQ=255)
                                                                                                   gCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATg                                                  <  2:241343/150‑1 (MQ=255)
                                                                                                       tGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGt                                              >  2:1818485/1‑150 (MQ=255)
                                                                                                          tGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGt                                           >  2:1282382/1‑150 (MQ=255)
                                                                                                          tGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGt                                           <  2:674733/150‑1 (MQ=255)
                                                                                                                   aGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATc                                  <  1:283522/150‑1 (MQ=255)
                                                                                                                   aGAAGGCGCGCGAGGCTGGCATTGCGGGCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGCTGCTGTCGTGGGTGCATc                                  >  2:1346597/1‑150 (MQ=255)
                                                                                                                   aGAAGGCGCGCGAGGCTGACATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGtt                                                                                                   >  1:1484780/1‑85 (MQ=255)
                                                                                                                   aGAAGGCGCGCGAGGCTGACATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGtt                                                                                                   <  2:1484780/85‑1 (MQ=255)
                                                                                                                    gAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCt                                 >  1:1662069/1‑150 (MQ=255)
                                                                                                                      aGGTGCGCGAGGCTGGCATTGCGGTTACCCGGGTGACCGACAGCTTCTGTTACTGGGCGGATGCCAATGCCGACGATGTGTTTCGTCTCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGa                               <  2:88836/150‑1 (MQ=255)
                                                                                                                      aGGCGCGCGAGGCTGGCATTGCGGTCCCCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATNTGa                               <  2:1187537/150‑1 (MQ=255)
                                                                                                                        gcgcgcGAGGCTGGCCTTGCGGTCCCCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATg                             <  1:809321/150‑1 (MQ=255)
                                                                                                                        gcgcgcGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATg                             <  1:243691/150‑1 (MQ=255)
                                                                                                                                 gCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATGGTCAACGAg                    <  2:1662069/150‑1 (MQ=255)
                                                                                                                                  cTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATGGTCAACGAgg                   <  1:1282382/150‑1 (MQ=255)
                                                                                                                                          tcGGTCACCAGGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCAc                                                            >  1:1451918/2‑101 (MQ=255)
                                                                                                                                          tcGGTCACCAGGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCAc                                                            <  2:1451918/100‑1 (MQ=255)
                                                                                                                                              tCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATGGTCAACGAGGTCTGCAAGAAGc       >  1:1033506/1‑150 (MQ=255)
                                                                                                                                                ccccTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATGGTCAACGAGGTCTGCAAGAAGCTc     <  2:754676/149‑1 (MQ=255)
                                                                                                                                                   cTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATGGTCAACGAGGTCTGCAAGAAGCTCGGa  >  1:1539495/1‑150 (MQ=255)
                                                                                                                                                   cTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCGGTCGTGGGTGCATCTGATGGTCAACGAGGTCTGCAAGAAGCTCGGa  >  1:414074/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGGTCGACATCAGCGCCGGGCACTTCGGCGAAGTGCTGCTGGGCCGTGCCGAAGACAGCGCCCTGGTGGTGTTCGAGGCGCGCCGTTATTCCCGCCATGCCCTGATGCTGTGCCAGAAGGCGCGCGAGGCTGGCATTGCGGTCACCCTGGTGACCGACACCTTCTGTGACTGGGCGGATGCCAATGCCGACGAGGTGTTCCGCATCCCCACCGAGTTCAACCTGTTCTGGGAATCCACCTCGACGATGCTGTCGTGGGTGCATCTGATGGTCAACGAGGTCTGCAAGAAGCTCGGA  >  NC_002947/4082637‑4082933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑