Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	321,440	A→G	6.6%	T278A (ACT→GCT)	aguA →	agmatine deiminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	321,440	0	A	G	6.6%	264.9 / 5.4	90	T278A (ACT→GCT)	aguA	agmatine deiminase
Reads supporting (aligned to +/- strand):  ref base A (42/42);  new base G (3/3);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

AAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGCCCCAAGCTTCGACGACCCGGCAGATGCCGAA  >  NC_002947/321294‑321586                                                                                                                                                   |                                                                                                                                                   aaGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTg                                                                                                                                                 >  2:32087/1‑150 (MQ=255)   gtgtTACTGGCCTGGACCGACGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGcc                                                                                                                                               >  2:55196/1‑150 (MQ=255)    tgtTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCg                                                                                                                                              >  1:486737/1‑150 (MQ=255)    tgtTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCg                                                                                                                                              >  1:1444990/1‑150 (MQ=255)      ttACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGaa                                                                                                                                            >  1:1260433/1‑150 (MQ=255)        aCTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAaga                                                                                                                                          <  2:1410467/150‑1 (MQ=255)        aCTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAaga                                                                                                                                          <  2:638700/150‑1 (MQ=255)                ggACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCg                                                                                                                                  >  2:1707390/1‑150 (MQ=255)                  cccGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGgt                                                                                                                                <  2:1328541/149‑1 (MQ=255)                  aCCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGCCACCTCTTTCCGCGAGTTTGCGGGTCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGgt                                                                                                                                <  1:1178211/150‑1 (MQ=255)                  aCCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGgt                                                                                                                                <  1:1730809/150‑1 (MQ=255)                     gatgatTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACt                                                                                                                                                  >  2:232448/1‑129 (MQ=255)                     gatgatTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACt                                                                                                                                                  <  1:232448/129‑1 (MQ=255)                          ttCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCa                                                                                                                        <  1:671908/150‑1 (MQ=255)                          ttCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCa                                                                                                                        >  1:419347/1‑150 (MQ=255)                           tCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCCTGGCTGTGCCTAAGTACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAg                                                                                                                       <  1:1564442/150‑1 (MQ=255)                             cAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAgg                                                                                                                      <  2:462392/149‑1 (MQ=255)                             cAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAgg                                                                                                                      >  1:462392/1‑149 (MQ=255)                             cAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGt                                                                                                                     >  1:898640/1‑150 (MQ=255)                              aaCGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGt                                                                                                                                        >  1:288558/1‑130 (MQ=255)                              aaCGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGt                                                                                                                                        <  2:288558/130‑1 (MQ=255)                              aaCGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTc                                                                                                                    <  1:1363546/150‑1 (MQ=255)                              aaCGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGGGTCGACCAGGTc                                                                                                                    >  1:391966/1‑150 (MQ=255)                              aaCGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTACCGAAGAGTGCGCCGGTGTCGACCAGGTc                                                                                                                    >  1:1838598/1‑150 (MQ=255)                                 gACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGcc                                                                                                                 >  1:167441/1‑150 (MQ=255)                                 gACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGcc                                                                                                                 >  1:1423183/1‑150 (MQ=255)                                     ccAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAaga                                                                                                                                          <  2:1439982/121‑1 (MQ=255)                                     ccAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAaga                                                                                                                                          >  1:1439982/1‑121 (MQ=255)                                      cAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGAc                                                                                                                          >  2:1826500/1‑136 (MQ=255)                                      cAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGAc                                                                                                                          <  1:1826500/136‑1 (MQ=255)                                               gcgcTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCg                                                                                                   >  2:1912575/1‑150 (MQ=255)                                                cgcTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGt                                                                                                  <  1:1342053/150‑1 (MQ=255)                                                 gcTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTg                                                                                                 <  2:419347/150‑1 (MQ=255)                                                   tGCCACGCGGCCATGGATGTGCTGAAGGACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGAc                                                                                               <  2:1880993/150‑1 (MQ=255)                                                               aTGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTgcgc                                                                                   >  2:77148/1‑150 (MQ=255)                                                               aTGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGAGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTgcgc                                                                                   >  2:547576/1‑150 (MQ=255)                                                                   aTGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGcc                                                                                                                 <  2:666937/116‑1 (MQ=255)                                                                   aTGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGcc                                                                                                                 >  1:666937/1‑116 (MQ=255)                                                                      tgCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCgg                                                                            <  2:167441/150‑1 (MQ=255)                                                                        cTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGt                                                                          >  2:1247304/1‑150 (MQ=255)                                                                        cTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGt                                                                          >  2:869315/1‑150 (MQ=255)                                                                          gaagaaCACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTc                                                                        <  1:32087/150‑1 (MQ=255)                                                                             gaaCACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGt                                                                                           >  1:433779/1‑128 (MQ=255)                                                                             gaaCACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGt                                                                                           <  2:433779/128‑1 (MQ=255)                                                                                  cgcgctACGCCAAGGGCCGCGCGTTCCTCGTGCACACGCTGGCGCTCCCGTGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGa                                                                <  2:1260433/148‑1 (MQ=255)                                                                                  cccGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGa                                                                >  2:433284/1‑150 (MQ=255)                                                                                   ccGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCGCTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAg                                                                                                     >  1:1544479/1‑112 (MQ=255)                                                                                   ccGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCGCTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAg                                                                                                     >  1:1544970/1‑112 (MQ=255)                                                                                   ccGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCGCTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAg                                                                                                     <  2:1546389/112‑1 (MQ=255)                                                                                   ccGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCGCTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAg                                                                                                     <  2:1544970/112‑1 (MQ=255)                                                                                   ccGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCGCTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAg                                                                                                     >  1:1546389/1‑112 (MQ=255)                                                                                   ccGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCGCTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAg                                                                                                     <  2:1544479/112‑1 (MQ=255)                                                                                   ccGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGt                                                                  <  2:257725/147‑1 (MQ=255)                                                                                   ccGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGt                                                                  >  1:257725/1‑147 (MQ=255)                                                                                   ccGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGaa                                                               <  2:1583579/150‑1 (MQ=255)                                                                                         cGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCt                                                         <  2:262136/150‑1 (MQ=255)                                                                                         cGCCAAGGGCCACGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCt                                                         <  1:1238938/150‑1 (MQ=255)                                                                                          gCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGAGAAGGTTTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTg                                                        >  1:1669403/1‑150 (MQ=255)                                                                                             aaGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGt                                                                  >  2:1216114/1‑137 (MQ=255)                                                                                             aaGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGt                                                                  <  1:1216114/137‑1 (MQ=255)                                                                                             aaGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGAtt                                                     >  2:389412/1‑150 (MQ=255)                                                                                               gggCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGt                                                   >  1:1835465/1‑150 (MQ=255)                                                                                                      gAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGACGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGt                                            <  2:982354/150‑1 (MQ=255)                                                                                                      gAGTTCATCGTGCACAAGATGCCGAGCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGt                                            <  2:562125/150‑1 (MQ=255)                                                                                                       aGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTg                                           <  1:869315/150‑1 (MQ=255)                                                                                                           cATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGcat                                       >  2:162055/1‑150 (MQ=255)                                                                                                             tCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGc                                                                              >  2:440309/1‑109 (MQ=255)                                                                                                             tCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGc                                                                              <  1:440309/109‑1 (MQ=255)                                                                                                               gTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTgg                                          >  1:1133362/1‑143 (MQ=255)                                                                                                               gTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTgg                                          <  2:1133362/143‑1 (MQ=255)                                                                                                                  cacaAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGcc                                <  1:744040/150‑1 (MQ=255)                                                                                                                  cacaAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGcc                                <  1:1912575/150‑1 (MQ=255)                                                                                                                  cacaAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGcc                                >  2:744040/1‑150 (MQ=255)                                                                                                                    caAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGcccc                              <  2:76679/150‑1 (MQ=255)                                                                                                                        aTGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGt                                                                  >  1:745207/1‑110 (MQ=255)                                                                                                                        aTGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGt                                                                  <  2:745207/110‑1 (MQ=255)                                                                                                                           ccGATCCCGGGCCCGCTGTTTGCCACTGGCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGGGCGTGACCCGTCGGTGCGCCGTGCGGGTACGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGCCCCAAGCTTc                       >  2:998387/1‑150 (MQ=255)                                                                                                                           ccGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGCCCCAAGCTTc                       <  1:162055/150‑1 (MQ=255)                                                                                                                            cGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGCCCCAAGCTTcg                      >  2:1823940/1‑150 (MQ=255)                                                                                                                             gATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGCCCCAAGCTTcga                     <  1:1157835/150‑1 (MQ=255)                                                                                                                             cggcccGGGCCCGCTGTTTGCCACTGGCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGACAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGCCCCAAGCTTcga                     <  1:28032/147‑1 (MQ=255)                                                                                                                                  cGGGCCCGCTGTTTGCCACTGCCGAAGCGCGACACGGTGTTGATCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGCCCCAAGCTTCGACGAcc                <  2:1590703/150‑1 (MQ=255)                                                                                                                                         gCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGcc                                >  2:183795/1‑127 (MQ=255)                                                                                                                                         gCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGcc                                <  1:183795/127‑1 (MQ=255)                                                                                                                                         gCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGCCCCAAGCTTCGACGACCCGGCAGa         >  1:1693515/1‑150 (MQ=255)                                                                                                                                           tGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGCCCCAAGCTTCGACGACCCGGCAGATg       >  2:1144386/1‑150 (MQ=255)                                                                                                                                             tttGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGCGCCATTGCGGTGGCATCATTGCCCCAAGCTTAAACGACCCGGCAGATGcc     >  1:1152118/1‑150 (MQ=255)                                                                                                                                                gCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGCCCCAAGCTTCGACGACCCGGCAGATGCCGaa  <  2:1835465/150‑1 (MQ=255)                                                                                                                                                  cACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTc                                                           >  2:503260/1‑91 (MQ=255)                                                                                                                                                  cACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTc                                                           <  1:503260/91‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   AAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGAGTGCGCCGGTGTCGACCAGGTCGCCGGCAGCCAGGAGCGTGACCCGTCGGTGCGCCTGGCGGGTTCGTACGTGAACTTCCTGATTGTTAACGGTGGCATCATTGCCCCAAGCTTCGACGACCCGGCAGATGCCGAA  >  NC_002947/321294‑321586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑