Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,134,021 T→A 9.0% A351A (GCA→GCT) ‡ PP_3638 ← acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,134,0210TA 9.0% 233.6 / 11.4 78A351A (GCA→GCT) ‡PP_3638acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (40/31);  new base A (3/4);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

CCCACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACCGC  >  NC_002947/4133873‑4134170
                                                                                                                                                    |                                                                                                                                                     
cccACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACgcctg                                                                                                                                                      >  2:644804/1‑150 (MQ=255)
   acacCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACgcctgcct                                                                                                                                                   >  2:1796954/1‑150 (MQ=255)
   acacCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACgcctgcct                                                                                                                                                   >  1:1432040/1‑150 (MQ=255)
      ccacTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGc                                                                                                                                                <  2:797771/150‑1 (MQ=255)
           ggggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGc                                                                                                                                           <  2:1574713/150‑1 (MQ=255)
            gggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCa                                                                                                                                          <  1:842200/150‑1 (MQ=255)
            gggCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCa                                                                                                                                          <  1:1749606/150‑1 (MQ=255)
               ccAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCAAATGCTGCCAACCACCCTTACATGGGTATCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGtt                                                                                                                                       >  1:1176828/1‑150 (MQ=255)
                cAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCTTGCCTGGCGTTGCAGTTc                                                                                                                                      <  1:1894609/150‑1 (MQ=255)
                cAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTc                                                                                                                                      >  2:1890523/1‑150 (MQ=255)
                cAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTc                                                                                                                                      <  1:644804/150‑1 (MQ=255)
                 aaGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCatgagc                                                                                                                                     <  2:1614865/150‑6 (MQ=255)
                   ggATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAATTCGgc                                                                                                                                   <  1:362126/150‑1 (MQ=255)
                     aTGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGgcgc                                                                                                                                 <  2:1432040/150‑1 (MQ=255)
                         tCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCaa                                                                                                                             >  1:155626/1‑150 (MQ=255)
                         tCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGCat                                                                                                                                         <  2:1267082/138‑2 (MQ=255)
                         tCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGCat                                                                                                                                         >  1:1267082/1‑137 (MQ=255)
                          cACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAAt                                                                                                                            >  2:587759/1‑150 (MQ=255)
                           aCGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAAtt                                                                                                                           <  1:670182/150‑1 (MQ=255)
                              aCCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCa                                                                                                                        <  2:555112/150‑1 (MQ=255)
                               ccTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCAc                                                                                                                       <  2:155626/150‑1 (MQ=255)
                                cTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCAcc                                                                                                                      >  2:1344062/1‑150 (MQ=255)
                                 tCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAGGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGCatga                                                                                                                                       <  1:922411/132‑4 (MQ=255)
                                 tCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAGGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGCatga                                                                                                                                       >  2:922411/1‑129 (MQ=255)
                                   aCCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAgg                                                                                                                   >  1:1202118/1‑150 (MQ=255)
                                     cTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCt                                                                                                                 >  1:846598/1‑150 (MQ=255)
                                            cAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGc                                                                                                                                           <  2:765144/117‑1 (MQ=255)
                                            cAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCAGGCAGGCGTTGc                                                                                                                                           >  1:765144/1‑117 (MQ=255)
                                             aGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGa                                                                                                         <  1:1585589/150‑1 (MQ=255)
                                                  cAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATc                                                                                                    >  1:1448072/1‑150 (MQ=255)
                                                     gTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGc                                                                                                 >  1:1752438/1‑150 (MQ=255)
                                                     gTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGc                                                                                                 >  2:793817/1‑150 (MQ=255)
                                                         aaCTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGa                                                                                             >  1:58492/1‑150 (MQ=255)
                                                           cTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAg                                                                                           <  1:1890523/150‑1 (MQ=255)
                                                            tGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAgg                                                                                          >  2:1673372/1‑150 (MQ=255)
                                                                ccAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGa                                                                                      <  2:1176828/150‑1 (MQ=255)
                                                                   gCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTc                                                                                   >  1:442503/1‑150 (MQ=255)
                                                                    cACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTcc                                                                                  >  1:1646989/1‑150 (MQ=255)
                                                                     aCCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATGGGCACGAAGGCCGACTccc                                                                                 >  2:644807/1‑150 (MQ=255)
                                                                       gtgctggCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGc                                                                               >  1:179591/2‑150 (MQ=255)
                                                                            gcCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcg                                                                          <  1:1658940/150‑1 (MQ=255)
                                                                              cAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggc                                                                        <  1:1796954/150‑1 (MQ=255)
                                                                               aaCCCCCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggcg                                                                       <  1:1271855/150‑1 (MQ=255)
                                                                                 ccaccCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCggg                                                                     <  1:587759/150‑1 (MQ=255)
                                                                                  caccCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGc                                                                    >  1:1225789/1‑150 (MQ=255)
                                                                                        cACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAAccc                                                              >  1:1117952/1‑150 (MQ=255)
                                                                                          cgcgGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCtt                                                            <  1:1346095/150‑1 (MQ=255)
                                                                                                    ccAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTc                                                  >  2:467841/1‑150 (MQ=255)
                                                                                                      aaGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGc                                                >  1:1195990/1‑150 (MQ=255)
                                                                                                      aaGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGc                                                >  1:1618120/1‑150 (MQ=255)
                                                                                                       aGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCa                                               >  1:1852824/1‑150 (MQ=255)
                                                                                                         cGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCaga                                             <  2:179591/150‑1 (MQ=255)
                                                                                                         cGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCATTCGCCGTATTCGCaga                                             <  1:15082/150‑1 (MQ=255)
                                                                                                          ggATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCagag                                            >  2:1745051/1‑150 (MQ=255)
                                                                                                           gATNTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAgg                                           >  2:1186420/1‑150 (MQ=255)
                                                                                                            aTGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTATTGATTACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTCCGCCGTATTCGCAGAGGt                                          >  1:1553700/1‑150 (MQ=255)
                                                                                                               tCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggcgg                                                                      <  1:71859/119‑1 (MQ=255)
                                                                                                               tCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggcgg                                                                      >  2:71859/1‑119 (MQ=255)
                                                                                                                cACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGc                                      <  2:1752438/150‑1 (MQ=255)
                                                                                                                 aCGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGcc                                     >  1:101578/1‑150 (MQ=255)
                                                                                                                   gggCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCtt                                   >  1:1082477/1‑150 (MQ=255)
                                                                                                                      ccTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTgcc                                >  1:966742/1‑150 (MQ=255)
                                                                                                                          cAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCa                            >  2:1169641/1‑150 (MQ=255)
                                                                                                                          cAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCa                            >  2:506605/1‑150 (MQ=255)
                                                                                                                            gCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCa                                               >  2:1835707/1‑129 (MQ=255)
                                                                                                                            gCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCa                                               <  1:1835707/129‑1 (MQ=255)
                                                                                                                              aaCACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTgg                        >  1:854003/1‑150 (MQ=255)
                                                                                                                                 acCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCt                     <  2:1117952/150‑1 (MQ=255)
                                                                                                                                 acCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCt                     >  2:588461/1‑150 (MQ=255)
                                                                                                                                   cgcgCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGc                   >  1:1784801/1‑150 (MQ=255)
                                                                                                                                     cgcTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCaa                 <  1:1344062/150‑1 (MQ=255)
                                                                                                                                      gcTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAAc                <  1:793817/150‑1 (MQ=255)
                                                                                                                                      gcTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAAc                >  2:1240282/1‑150 (MQ=255)
                                                                                                                                          aTGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCa            >  1:451133/1‑150 (MQ=255)
                                                                                                                                           tGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg           <  2:58492/150‑1 (MQ=255)
                                                                                                                                           tGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAg           <  2:442503/150‑1 (MQ=255)
                                                                                                                                            gCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggcg                                                                       <  1:747425/89‑1 (MQ=255)
                                                                                                                                            gCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATcggcg                                                                       >  2:747425/1‑89 (MQ=255)
                                                                                                                                             cAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGtt         >  1:1557583/1‑150 (MQ=255)
                                                                                                                                              aaCGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTg        >  2:582025/1‑150 (MQ=255)
                                                                                                                                                cGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTa      <  2:1784801/150‑1 (MQ=255)
                                                                                                                                                 gcctgcctGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGCCGACAGCTTTGCCGCCACTGGCCTGCAACTCCAGTTGTAc     >  2:627679/1‑150 (MQ=255)
                                                                                                                                                  cctgcctgGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCa            >  1:545659/1‑142 (MQ=255)
                                                                                                                                                  cctgcctgGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCa            <  2:545659/142‑1 (MQ=255)
                                                                                                                                                    tgcctgGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACcgc  >  1:1711677/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCCACACCACTGGGGCCAAGGATGCTCACGACCTCACCTGGTGCCAGCTGCAGGTCGAACTGCGCCAGCACCTGCTGCCAACCACCCTCACGCGGGTAGCCCAAGCGGATGTCACGGGCCTCCAGCAACACCGCGCTCATGCAACGCCTGCCTGGCGTTGCAGTTCGGCGCGCAATTGCACCAGGCTGGGCGTGACGATCGGCACGAAGGCCGACTCCCGCCATCGGCGGGCAAACCCTTCGCCGTATTCGCAGAGGTAAGCCTTGCCGCCACTGGCCTGCAACTCCAGTTGTACCGC  >  NC_002947/4133873‑4134170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑