Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,248,398 C→T 8.2% R558H (CGC→CAC)  PP_3724 ← acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,248,3980CT 8.2% 166.5 / 4.5 61R558H (CGC→CAC) PP_3724acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (34/22);  new base T (3/2);  total (37/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

GCCGCCGGCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTT  >  NC_002947/4248253‑4248545
                                                                                                                                                 |                                                                                                                                                   
gccgccGGCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCAt                                                                                                                                                 >  1:1227595/1‑150 (MQ=255)
          ccGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTACGCTAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGt                                                                                                                                       <  1:1455948/150‑1 (MQ=255)
            gATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGc                                                                                                                                     >  1:821603/1‑150 (MQ=255)
            gATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGc                                                                                                                                     <  1:2187/150‑1 (MQ=255)
             aTCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCa                                                                                                                                    <  1:307011/150‑1 (MQ=255)
                 ccAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCtt                                                                                                                                >  1:905235/1‑150 (MQ=255)
                    aGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCa                                                                                                                             <  2:478135/150‑1 (MQ=255)
                    aGGCCAGGGCAAAGTTGTTTTCACGCTTGACGCCCACTATGAGCTCGGCCAAGAGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCa                                                                                                                             <  1:994454/150‑1 (MQ=255)
                            gCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCgg                                                                                                                     <  1:109275/150‑1 (MQ=255)
                                aGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCAt                                                                                                                 <  2:1596243/150‑1 (MQ=255)
                                  ttgttTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCATCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGCGCAGCTTCCAGGGCGCGGGCATcg                                                                                                               >  1:1121951/1‑150 (MQ=255)
                                     ttttCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCgcgc                                                                                                            >  1:556238/1‑150 (MQ=255)
                                       ttCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAg                                                                                                          <  2:821603/150‑1 (MQ=255)
                                          aCGCTTGATGCGCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGGAGGtt                                                                                                       >  1:940611/1‑150 (MQ=255)
                                            gCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCa                                                                                                     >  1:1055510/1‑150 (MQ=255)
                                                  tGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCca                                                                                               >  2:1117882/1‑150 (MQ=255)
                                                  tGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCca                                                                                               >  1:658887/1‑150 (MQ=255)
                                                  tGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTCCAACGCca                                                                                               >  1:1327475/1‑150 (MQ=255)
                                                  tGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAAAGTGTGTTCATCGCGCAGGTTCAACGCca                                                                                               <  1:1204648/150‑1 (MQ=255)
                                                      cACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCCACCTCCAGCTAGCCGCAGTTCCGCAACGCTTGTTAGACAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGc                                                                                           <  1:224140/150‑1 (MQ=255)
                                                               cTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTc                                                                                  <  1:795551/150‑1 (MQ=255)
                                                                tCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAg                                                                                                          <  2:1244604/125‑1 (MQ=255)
                                                                tCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAg                                                                                                          >  1:1244604/1‑125 (MQ=255)
                                                                   gCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCAGACGACCCCGCAAAACATGCGAGTCTTAGTCAGCGAAGCGCTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCCTCGAGCACTTTTAACGCCACAGCACCAGCTTCGGTc                                                                              >  1:330179/1‑150 (MQ=255)
                                                                   gCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTc                                                                              <  1:466903/150‑1 (MQ=255)
                                                                      aaGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTtg                                                                           >  2:1104783/1‑150 (MQ=255)
                                                                       aGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTtgt                                                                          >  1:1362877/1‑150 (MQ=255)
                                                                          gcggcTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCg                                                                       >  2:903185/1‑150 (MQ=255)
                                                                                   ccATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTgcg                                                              >  1:1175379/1‑150 (MQ=255)
                                                                                      tGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTgcgcgc                                                           >  2:121899/1‑150 (MQ=255)
                                                                                      tGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTgcgcgc                                                           >  2:1913897/1‑150 (MQ=255)
                                                                                      tGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTgcgcgc                                                           >  2:1914676/1‑150 (MQ=255)
                                                                                         gCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGa                                                        >  1:1505079/1‑150 (MQ=255)
                                                                                          cTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCGTCGCGCAGTTTCAACTCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGAc                                                       <  2:884140/150‑1 (MQ=255)
                                                                                           tCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGAcc                                                      >  1:254077/1‑150 (MQ=255)
                                                                                             gAGCAGCACCTGCTCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCGGCTGCGCGCTGACCGc                                                    <  2:1055510/150‑1 (MQ=255)
                                                                                               gcagcaCCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCt                                                  >  2:1832562/1‑150 (MQ=255)
                                                                                                    aCCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGt                                             >  1:1757242/1‑150 (MQ=255)
                                                                                                         aTCAAACGCAACCTGCGGGGCGTAGTCAGCGATGAACAGGCGCATCAGCACCAGTGAAGCTTCCAGGGCACGGCCATCGCGCAGTTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCaa                                        >  2:216068/1‑150 (MQ=255)
                                                                                                            aaaCGCAACCTGCGGGGCGTAGTCAGCGATAAACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAggg                                     >  1:366410/1‑150 (MQ=255)
                                                                                                             tcgctgcccTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTGGTGCGTCAGCTGCGCGCTGACCGCCTTCAGTTCCAAgggg                                    >  2:1040436/8‑150 (MQ=255)
                                                                                                             aaCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCt                                                                 <  1:1356379/121‑1 (MQ=255)
                                                                                                             aaCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCt                                                                 >  2:1356379/1‑121 (MQ=255)
                                                                                                             aaCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAgggg                                    >  1:1881928/1‑150 (MQ=255)
                                                                                                              aCGCAACCTGCGGGGCGTAGACAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGt                                   >  2:1414700/1‑150 (MQ=255)
                                                                                                                  aaCCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCcaca                                                                                             >  1:1214312/1‑88 (MQ=255)
                                                                                                                  aaCCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCcaca                                                                                             <  2:1214312/88‑1 (MQ=255)
                                                                                                                   aCCTGCGGTGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGTGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCTGTCTTGTGCGGCAGCTGCGCGCTGACCGCTTTCAGTACCAAGGGGTAACCg                              <  2:87913/150‑1 (MQ=255)
                                                                                                                      tGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGAcg                           >  2:1172214/1‑150 (MQ=255)
                                                                                                                      tGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGAcg                           >  2:297810/1‑150 (MQ=255)
                                                                                                                               tAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGgcggcg                  <  2:1362877/150‑1 (MQ=255)
                                                                                                                                  tCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCt               <  2:1448658/150‑1 (MQ=255)
                                                                                                                                  tCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGAGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCTCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCt               <  2:489424/150‑1 (MQ=255)
                                                                                                                                    gaagctGCACTGGTGCAGATGCGCCAGTGCATCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCCGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAACGCGTCACCGACGCGCGCGGCGTCTGc             >  2:775140/6‑150 (MQ=255)
                                                                                                                                      agctGCACTGGTGCAGATGCGCCAGTGCATCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAg                                       <  2:1001348/119‑1 (MQ=38)
                                                                                                                                      agctGCACTGGTGCAGATGCGCCAGTGCATCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAg                                       >  1:1001348/4‑122 (MQ=38)
                                                                                                                                         tGCACTGGTGCAGATGCGCCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACgcgggcg                     >  2:1349511/1‑137 (MQ=255)
                                                                                                                                         tGCACTGGTGCAGATGCGCCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACgcgggcg                     <  1:1349511/137‑1 (MQ=255)
                                                                                                                                            aCTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGgcgc     >  1:868587/1‑150 (MQ=255)
                                                                                                                                             cTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGcgcg    >  2:1713388/1‑150 (MQ=255)
                                                                                                                                               ggCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGtt  <  1:903185/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCCGCCGGCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTT  >  NC_002947/4248253‑4248545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑