Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,248,402	T→G	7.4%	M557L (ATG→CTG)	PP_3724 ←	acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,248,402	0	T	G	7.4%	162.9 / 4.6	62	M557L (ATG→CTG)	PP_3724	acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (33/24);  new base G (3/2);  total (36/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GCCGCCGGCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGC  >  NC_002947/4248253‑4248550                                                                                                                                                      |                                                                                                                                                     gccgccGGCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCAt                                                                                                                                                      >  1:1227595/1‑150 (MQ=255)           ccGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTACGCTAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGt                                                                                                                                            <  1:1455948/150‑1 (MQ=255)             gATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGc                                                                                                                                          >  1:821603/1‑150 (MQ=255)             gATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGc                                                                                                                                          <  1:2187/150‑1 (MQ=255)              aTCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCa                                                                                                                                         <  1:307011/150‑1 (MQ=255)                  ccAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCtt                                                                                                                                     >  1:905235/1‑150 (MQ=255)                     aGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCa                                                                                                                                  <  2:478135/150‑1 (MQ=255)                     aGGCCAGGGCAAAGTTGTTTTCACGCTTGACGCCCACTATGAGCTCGGCCAAGAGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCa                                                                                                                                  <  1:994454/150‑1 (MQ=255)                             gCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCgg                                                                                                                          <  1:109275/150‑1 (MQ=255)                                 aGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCAt                                                                                                                      <  2:1596243/150‑1 (MQ=255)                                   ttgttTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCATCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGCGCAGCTTCCAGGGCGCGGGCATcg                                                                                                                    >  1:1121951/1‑150 (MQ=255)                                      ttttCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCgcgc                                                                                                                 >  1:556238/1‑150 (MQ=255)                                        ttCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAg                                                                                                               <  2:821603/150‑1 (MQ=255)                                           aCGCTTGATGCGCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGGAGGtt                                                                                                            >  1:940611/1‑150 (MQ=255)                                             gCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCa                                                                                                          >  1:1055510/1‑150 (MQ=255)                                                   tGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCca                                                                                                    >  2:1117882/1‑150 (MQ=255)                                                   tGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCca                                                                                                    >  1:658887/1‑150 (MQ=255)                                                   tGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTCCAACGCca                                                                                                    >  1:1327475/1‑150 (MQ=255)                                                   tGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAAAGTGTGTTCATCGCGCAGGTTCAACGCca                                                                                                    <  1:1204648/150‑1 (MQ=255)                                                       cACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCCACCTCCAGCTAGCCGCAGTTCCGCAACGCTTGTTAGACAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGc                                                                                                <  1:224140/150‑1 (MQ=255)                                                                cTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTc                                                                                       <  1:795551/150‑1 (MQ=255)                                                                 tCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAg                                                                                                               <  2:1244604/125‑1 (MQ=255)                                                                 tCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAg                                                                                                               >  1:1244604/1‑125 (MQ=255)                                                                    gCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCAGACGACCCCGCAAAACATGCGAGTCTTAGTCAGCGAAGCGCTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCCTCGAGCACTTTTAACGCCACAGCACCAGCTTCGGTc                                                                                   >  1:330179/1‑150 (MQ=255)                                                                    gCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTc                                                                                   <  1:466903/150‑1 (MQ=255)                                                                       aaGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTtg                                                                                >  2:1104783/1‑150 (MQ=255)                                                                        aGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTtgt                                                                               >  1:1362877/1‑150 (MQ=255)                                                                           gcggcTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCg                                                                            >  2:903185/1‑150 (MQ=255)                                                                                    ccATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTgcg                                                                   >  1:1175379/1‑150 (MQ=255)                                                                                       tGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTgcgcgc                                                                >  2:1914676/1‑150 (MQ=255)                                                                                       tGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTgcgcgc                                                                >  2:1913897/1‑150 (MQ=255)                                                                                       tGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTgcgcgc                                                                >  2:121899/1‑150 (MQ=255)                                                                                          gCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGa                                                             >  1:1505079/1‑150 (MQ=255)                                                                                           cTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCGTCGCGCAGTTTCAACTCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGAc                                                            <  2:884140/150‑1 (MQ=255)                                                                                            tCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGAcc                                                           >  1:254077/1‑150 (MQ=255)                                                                                              gAGCAGCACCTGCTCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCGGCTGCGCGCTGACCGc                                                         <  2:1055510/150‑1 (MQ=255)                                                                                                gcagcaCCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCt                                                       >  2:1832562/1‑150 (MQ=255)                                                                                                     aCCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGt                                                  >  1:1757242/1‑150 (MQ=255)                                                                                                          aTCAAACGCAACCTGCGGGGCGTAGTCAGCGATGAACAGGCGCATCAGCACCAGTGAAGCTTCCAGGGCACGGCCATCGCGCAGTTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCaa                                             >  2:216068/1‑150 (MQ=255)                                                                                                             aaaCGCAACCTGCGGGGCGTAGTCAGCGATAAACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAggg                                          >  1:366410/1‑150 (MQ=255)                                                                                                              tcgctgcccTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTGGTGCGTCAGCTGCGCGCTGACCGCCTTCAGTTCCAAgggg                                         >  2:1040436/8‑150 (MQ=255)                                                                                                              aaCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCt                                                                      >  2:1356379/1‑121 (MQ=255)                                                                                                              aaCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCt                                                                      <  1:1356379/121‑1 (MQ=255)                                                                                                              aaCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAgggg                                         >  1:1881928/1‑150 (MQ=255)                                                                                                               aCGCAACCTGCGGGGCGTAGACAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGt                                        >  2:1414700/1‑150 (MQ=255)                                                                                                                   aaCCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCcaca                                                                                                  <  2:1214312/88‑1 (MQ=255)                                                                                                                   aaCCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCcaca                                                                                                  >  1:1214312/1‑88 (MQ=255)                                                                                                                    aCCTGCGGTGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGTGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCTGTCTTGTGCGGCAGCTGCGCGCTGACCGCTTTCAGTACCAAGGGGTAACCg                                   <  2:87913/150‑1 (MQ=255)                                                                                                                       tGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGAcg                                >  2:1172214/1‑150 (MQ=255)                                                                                                                       tGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGAcg                                >  2:297810/1‑150 (MQ=255)                                                                                                                                tAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGgcggcg                       <  2:1362877/150‑1 (MQ=255)                                                                                                                                   tCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCt                    <  2:1448658/150‑1 (MQ=255)                                                                                                                                   tCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGAGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCTCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCt                    <  2:489424/150‑1 (MQ=255)                                                                                                                                     gaagctGCACTGGTGCAGATGCGCCAGTGCATCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCCGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAACGCGTCACCGACGCGCGCGGCGTCTGc                  >  2:775140/6‑150 (MQ=255)                                                                                                                                       agctGCACTGGTGCAGATGCGCCAGTGCATCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAg                                            >  1:1001348/4‑122 (MQ=38)                                                                                                                                       agctGCACTGGTGCAGATGCGCCAGTGCATCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAg                                            <  2:1001348/119‑1 (MQ=38)                                                                                                                                          tGCACTGGTGCAGATGCGCCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACgcgggcg                          >  2:1349511/1‑137 (MQ=255)                                                                                                                                          tGCACTGGTGCAGATGCGCCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACgcgggcg                          <  1:1349511/137‑1 (MQ=255)                                                                                                                                             aCTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGgcgc          >  1:868587/1‑150 (MQ=255)                                                                                                                                              cTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGcgcg         >  2:1713388/1‑150 (MQ=255)                                                                                                                                                ggCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGtt       <  1:903185/150‑1 (MQ=255)                                                                                                                                                   gcATCTGCACCAGTGCAGCTTCCGGGGGGTGGGCATCGGGCAGGTTCAACGCCACAGCACCAGCTTCGGTCGTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCgg    <  1:1772433/150‑1 (MQ=255)                                                                                                                                                     aTCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGc  <  1:1414700/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     GCCGCCGGCACCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGC  >  NC_002947/4248253‑4248550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑