Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,248,407	A→G	7.9%	V555A (GTG→GCG)	PP_3724 ←	acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,248,407	0	A	G	7.9%	177.2 / 4.6	63	V555A (GTG→GCG)	PP_3724	acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (34/24);  new base G (3/2);  total (37/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

CCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTC  >  NC_002947/4248263‑4248556                                                                                                                                                 |                                                                                                                                                      ccGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTACGCTAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGt                                                                                                                                                  <  1:1455948/150‑1 (MQ=255)   gATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGc                                                                                                                                                >  1:821603/1‑150 (MQ=255)   gATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGc                                                                                                                                                <  1:2187/150‑1 (MQ=255)    aTCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCa                                                                                                                                               <  1:307011/150‑1 (MQ=255)        ccAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCtt                                                                                                                                           >  1:905235/1‑150 (MQ=255)           aGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCa                                                                                                                                        <  2:478135/150‑1 (MQ=255)           aGGCCAGGGCAAAGTTGTTTTCACGCTTGACGCCCACTATGAGCTCGGCCAAGAGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCa                                                                                                                                        <  1:994454/150‑1 (MQ=255)                   gCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCgg                                                                                                                                <  1:109275/150‑1 (MQ=255)                       aGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCAt                                                                                                                            <  2:1596243/150‑1 (MQ=255)                         ttgttTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCATCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGCGCAGCTTCCAGGGCGCGGGCATcg                                                                                                                          >  1:1121951/1‑150 (MQ=255)                            ttttCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCgcgc                                                                                                                       >  1:556238/1‑150 (MQ=255)                              ttCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAg                                                                                                                     <  2:821603/150‑1 (MQ=255)                                 aCGCTTGATGCGCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGGAGGtt                                                                                                                  >  1:940611/1‑150 (MQ=255)                                   gCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCa                                                                                                                >  1:1055510/1‑150 (MQ=255)                                         tGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCca                                                                                                          >  2:1117882/1‑150 (MQ=255)                                         tGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCca                                                                                                          >  1:658887/1‑150 (MQ=255)                                         tGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTCCAACGCca                                                                                                          >  1:1327475/1‑150 (MQ=255)                                         tGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAAAGTGTGTTCATCGCGCAGGTTCAACGCca                                                                                                          <  1:1204648/150‑1 (MQ=255)                                             cACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCCACCTCCAGCTAGCCGCAGTTCCGCAACGCTTGTTAGACAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGc                                                                                                      <  1:224140/150‑1 (MQ=255)                                                      cTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTc                                                                                             <  1:795551/150‑1 (MQ=255)                                                       tCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAg                                                                                                                     <  2:1244604/125‑1 (MQ=255)                                                       tCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAg                                                                                                                     >  1:1244604/1‑125 (MQ=255)                                                          gCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCAGACGACCCCGCAAAACATGCGAGTCTTAGTCAGCGAAGCGCTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCCTCGAGCACTTTTAACGCCACAGCACCAGCTTCGGTc                                                                                         >  1:330179/1‑150 (MQ=255)                                                          gCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTc                                                                                         <  1:466903/150‑1 (MQ=255)                                                             aaGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTtg                                                                                      >  2:1104783/1‑150 (MQ=255)                                                              aGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTtgt                                                                                     >  1:1362877/1‑150 (MQ=255)                                                                 gcggcTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCg                                                                                  >  2:903185/1‑150 (MQ=255)                                                                          ccATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTgcg                                                                         >  1:1175379/1‑150 (MQ=255)                                                                             tGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTgcgcgc                                                                      >  2:1914676/1‑150 (MQ=255)                                                                             tGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTgcgcgc                                                                      >  2:1913897/1‑150 (MQ=255)                                                                             tGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTgcgcgc                                                                      >  2:121899/1‑150 (MQ=255)                                                                                gCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGa                                                                   >  1:1505079/1‑150 (MQ=255)                                                                                 cTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCGTCGCGCAGTTTCAACTCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGAc                                                                  <  2:884140/150‑1 (MQ=255)                                                                                  tCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGAcc                                                                 >  1:254077/1‑150 (MQ=255)                                                                                    gAGCAGCACCTGCTCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCGGCTGCGCGCTGACCGc                                                               <  2:1055510/150‑1 (MQ=255)                                                                                      gcagcaCCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCt                                                             >  2:1832562/1‑150 (MQ=255)                                                                                           aCCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGt                                                        >  1:1757242/1‑150 (MQ=255)                                                                                                aTCAAACGCAACCTGCGGGGCGTAGTCAGCGATGAACAGGCGCATCAGCACCAGTGAAGCTTCCAGGGCACGGCCATCGCGCAGTTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCaa                                                   >  2:216068/1‑150 (MQ=255)                                                                                                   aaaCGCAACCTGCGGGGCGTAGTCAGCGATAAACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAggg                                                >  1:366410/1‑150 (MQ=255)                                                                                                    tcgctgcccTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTGGTGCGTCAGCTGCGCGCTGACCGCCTTCAGTTCCAAgggg                                               >  2:1040436/8‑150 (MQ=255)                                                                                                    aaCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCt                                                                            >  2:1356379/1‑121 (MQ=255)                                                                                                    aaCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCt                                                                            <  1:1356379/121‑1 (MQ=255)                                                                                                    aaCGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAgggg                                               >  1:1881928/1‑150 (MQ=255)                                                                                                     aCGCAACCTGCGGGGCGTAGACAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGt                                              >  2:1414700/1‑150 (MQ=255)                                                                                                         aaCCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCcaca                                                                                                        <  2:1214312/88‑1 (MQ=255)                                                                                                         aaCCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCcaca                                                                                                        >  1:1214312/1‑88 (MQ=255)                                                                                                          aCCTGCGGTGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGTGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCTGTCTTGTGCGGCAGCTGCGCGCTGACCGCTTTCAGTACCAAGGGGTAACCg                                         <  2:87913/150‑1 (MQ=255)                                                                                                             tGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGAcg                                      >  2:1172214/1‑150 (MQ=255)                                                                                                             tGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGAcg                                      >  2:297810/1‑150 (MQ=255)                                                                                                                      tAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGgcggcg                             <  2:1362877/150‑1 (MQ=255)                                                                                                                         tCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCt                          <  2:1448658/150‑1 (MQ=255)                                                                                                                         tCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGAGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCTCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCt                          <  2:489424/150‑1 (MQ=255)                                                                                                                           gaagctGCACTGGTGCAGATGCGCCAGTGCATCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCCGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAACGCGTCACCGACGCGCGCGGCGTCTGc                        >  2:775140/6‑150 (MQ=255)                                                                                                                             agctGCACTGGTGCAGATGCGCCAGTGCATCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAg                                                  <  2:1001348/119‑1 (MQ=38)                                                                                                                             agctGCACTGGTGCAGATGCGCCAGTGCATCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAg                                                  >  1:1001348/4‑122 (MQ=38)                                                                                                                                tGCACTGGTGCAGATGCGCCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACgcgggcg                                <  1:1349511/137‑1 (MQ=255)                                                                                                                                tGCACTGGTGCAGATGCGCCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACgcgggcg                                >  2:1349511/1‑137 (MQ=255)                                                                                                                                   aCTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGgcgc                >  1:868587/1‑150 (MQ=255)                                                                                                                                    cTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGcgcg               >  2:1713388/1‑150 (MQ=255)                                                                                                                                      ggCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGtt             <  1:903185/150‑1 (MQ=255)                                                                                                                                         gcATCTGCACCAGTGCAGCTTCCGGGGGGTGGGCATCGGGCAGGTTCAACGCCACAGCACCAGCTTCGGTCGTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCgg          <  1:1772433/150‑1 (MQ=255)                                                                                                                                           aTCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGc        <  1:1414700/150‑1 (MQ=255)                                                                                                                                              tGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTa     >  2:1385273/1‑150 (MQ=255)                                                                                                                                                 aCCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCt                                                             >  2:822655/1‑91 (MQ=255)                                                                                                                                                 aCCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCt                                                             <  1:822655/91‑1 (MQ=255)                                                                                                                                                 aCCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTc  >  1:1062657/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                      CCGATCACCAAGGCCAGGGCAAAGTTGTTTTCACGCTTGATGCCCACTATGAGCTCGGCCAAGGGCGGCTCGGCCATGGGCTCGAGCAGCACCTGATCAAACGCAACCTGCGGGGCGTAGTCAGCGATGCACTGGCGCATCTGCACCAGTGCAGCTTCCAGGGCGCGGGCATCGCGCAGGTTCAACGCCACAGCACCAGCTTCGGTCTTGTGCGGCAGCTGCGCGCTGACCGCCTTCAGTACCAAGGGGTAACCGACGCGGGCGGCGTCTGCCACGGCGCGTTCGGGCGTACTC  >  NC_002947/4248263‑4248556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑