Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,399,610	T→G	6.6%	R108R (CGA→CGC)	PP_3878 ←	minor capsid protein C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,399,610	0	T	G	6.6%	188.8 / 2.7	68	R108R (CGA→CGC)	PP_3878	minor capsid protein C
Reads supporting (aligned to +/- strand):  ref base T (38/25);  new base G (3/2);  total (41/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCTGCTCAGCCT  >  NC_002947/4399465‑4399755                                                                                                                                                  |                                                                                                                                                  ccAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCa                                                                                                                                               <  1:275648/150‑1 (MQ=255)      ccGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCGAGCAGCCCt                                                                                                                                          <  2:1599676/150‑1 (MQ=255)               ggTTTGATCTTGGTCGCGGCCCGAATTTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCCCTGTTGAGCACAATGTGTATGGATTTGGCTACAGTCATTGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTg                                                                                                                                 <  1:1279228/150‑1 (MQ=255)                 tttGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGCCGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGt                                                                                                                               >  1:204626/1‑150 (MQ=255)                 tttGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGt                                                                                                                               >  1:714631/1‑150 (MQ=255)                 tttGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGt                                                                                                                               >  1:562541/1‑150 (MQ=255)                     aTCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATTGGGTCAGTGATGGCCTTATTCAGGGCTGCGCGCAGCCCTTCATAGCTGGTCATg                                                                                                                           <  1:1889529/150‑1 (MQ=255)                     aTCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATg                                                                                                                           <  2:1229667/150‑1 (MQ=255)                            tCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGc                                                                                                                    >  1:47543/1‑150 (MQ=255)                              gcgGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAg                                                                                                                  <  2:607240/150‑1 (MQ=255)                                 gCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCg                                                                                                               >  1:1686942/1‑150 (MQ=255)                                  cccGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGt                                                                                                              <  2:570370/150‑1 (MQ=255)                                   ccGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGtt                                                                                                             >  1:1778255/1‑150 (MQ=255)                                    cGANTGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGGCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTc                                                                                                            >  2:1193405/1‑150 (MQ=255)                                        tGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGt                                                                                                        <  2:47543/150‑1 (MQ=255)                                             gccgccAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTg                                                                                                   >  2:1770385/1‑150 (MQ=255)                                              ccgccAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGc                                                                                                  <  2:1869454/150‑1 (MQ=255)                                                gccAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGATAGGGCCTTATTCAGGGCTGCTCGCAGCATTCCATATTTTGTCGTGGTCTCGCAGGCGTTCAAGTGGCTGCcg                                                                                                >  1:888577/1‑150 (MQ=255)                                                 ccAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGCGGTCGCAGCCCGTCAGGGGGCGGCcgc                                                                                               >  2:1853312/1‑150 (MQ=255)                                                   aGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCa                                                                                                                   <  1:1570944/128‑1 (MQ=255)                                                   aGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCa                                                                                                                   >  2:1570944/1‑128 (MQ=255)                                                    gTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCACGTGCCTGCcgctag                                                                                            >  1:1461777/1‑147 (MQ=255)                                                    gTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGAc                                                                                            >  2:570834/1‑150 (MQ=255)                                                              gcCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAAc                                                                                  <  2:1804711/150‑1 (MQ=255)                                                                cGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGc                                                                                <  1:1304421/150‑1 (MQ=255)                                                                  aCGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCa                                                                              >  2:669111/1‑150 (MQ=255)                                                                   cGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCAt                                                                             <  1:255801/150‑1 (MQ=255)                                                                     gcgcTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATgg                                                                           >  1:225980/1‑150 (MQ=255)                                                                       gcTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGGTGCTCGCAGCCCTTCATAGCTGGGCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACGCACAAGAACGCCATGGaa                                                                         >  1:360355/1‑149 (MQ=255)                                                                         agcggacTGGGGTGTTAATGTCAAAGAGAATGTGTTCGAGCTTGGGGACCGAGTTGGGCCTACTCAGTGATGATAAAAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGGCgg                                                                       >  2:1316573/7‑150 (MQ=255)                                                                          gccgccTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACggg                                                                      <  2:714631/150‑1 (MQ=255)                                                                          gccgccTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGCCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACggg                                                                      <  1:1518858/150‑1 (MQ=255)                                                                               cTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCa                                                                 <  2:861278/150‑1 (MQ=255)                                                                                tGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTc                                                                                                                                        <  2:957773/78‑1 (MQ=255)                                                                                tGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTc                                                                                                                                        >  1:957773/1‑78 (MQ=255)                                                                                tGGGCTGTCAATGTCGAGCACAATGTGTTCGAAAGTGGGTTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTTATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTAACAAGAACGCCATGGACGGGGATCAc                                                                >  1:1590122/1‑150 (MQ=255)                                                                                      gTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCGGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCcac                                                          <  2:562541/150‑1 (MQ=255)                                                                                       tCAATGTCGAGCACAATGTGTTCGACCATGGGGCCACTGCTGCCACACTGCAGGGCTGCTCGCAGCCCTTTGAAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGAATCACAAGAACACCATGGACGGGGATCACCTCcaca                                                         >  1:1773395/1‑150 (MQ=255)                                                                                        cAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCacac                                                        <  1:669111/150‑1 (MQ=255)                                                                                         aaTGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAACCGTGCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACAcc                                                       >  1:1756566/1‑150 (MQ=255)                                                                                          aTGTCGATCACAATGTGTGCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACAccc                                                      <  2:204626/150‑1 (MQ=255)                                                                                            gTCGAACACATTGTGCTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCTTGGACGGGGATCACCTCCACACCCGt                                                    >  2:1149834/1‑150 (MQ=255)                                                                                              cGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTAAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGACCACCTCCACACCCGTCt                                                  <  2:324495/150‑1 (MQ=255)                                                                                               gAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAg                                                                                                         <  1:1828821/94‑1 (MQ=255)                                                                                               gAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAg                                                                                                         >  2:1828821/1‑94 (MQ=255)                                                                                               gAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTg                                                 >  2:734500/1‑150 (MQ=255)                                                                                                    caATGTGTTCGACCACGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGa                                            <  2:1391243/150‑1 (MQ=255)                                                                                                     aaTGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGTAGGCGTTCAAGTGGCTGACGCGACACACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGAt                                           >  1:1069369/1‑150 (MQ=255)                                                                                                       tgtgTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGtc                                                                                                                        <  1:286162/71‑1 (MQ=255)                                                                                                       tgtgTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGtc                                                                                                                        >  2:286162/1‑71 (MQ=255)                                                                                                           ttCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATggcggc                                     >  1:1173747/1‑150 (MQ=255)                                                                                                              gACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGgcggcgcg                                  >  1:1714057/1‑150 (MQ=255)                                                                                                                ccATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCa                                                                                                                                               <  1:1025970/39‑1 (MQ=255)                                                                                                                ccATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCa                                                                                                                                               >  2:1025970/1‑39 (MQ=255)                                                                                                                 cATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGcggcgcggcg                               >  2:713677/1‑150 (MQ=255)                                                                                                                    ggggTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCCCAAGAAAGCCATGTACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTg                            >  1:416521/1‑150 (MQ=255)                                                                                                                                ggCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCgcggcg                >  1:1567206/1‑150 (MQ=255)                                                                                                                                  ccTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGAt              >  1:991349/1‑150 (MQ=255)                                                                                                                                   cTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACCAGAACGCCATGGGCGGGGGTCACCTCCACACCCGTCTGGGCGATGGCGGCGCGCGGCTGTTCTTCGAGGCGATc             >  2:235767/1‑150 (MQ=255)                                                                                                                                   cTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATc             >  1:376139/1‑150 (MQ=255)                                                                                                                                   cTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATc             <  2:1313455/150‑1 (MQ=255)                                                                                                                                    ttATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATcc            >  1:1862418/1‑150 (MQ=255)                                                                                                                                    ttATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATcc            >  2:100555/1‑150 (MQ=255)                                                                                                                                     tatgaaGGGCTGCGAGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCt           >  2:1636118/6‑150 (MQ=255)                                                                                                                                      atgaaGGGCTGCGAGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGCGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCTg          >  1:375149/5‑150 (MQ=255)                                                                                                                                        gaaGGGCTGCGAGCAGCCCTGAATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAAAGAAATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCTGCt        >  1:894757/3‑150 (MQ=255)                                                                                                                                          aGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCTGCTCa      >  2:473170/1‑150 (MQ=255)                                                                                                                                          aGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCTGCTCa      <  2:1714057/150‑1 (MQ=255)                                                                                                                                              ctgctCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCTGCTCAGCCt  >  1:3665/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGGGCGATGGCGGCGCGGCGCTGTTCTTCGCGGCGATCCTGCTCAGCCT  >  NC_002947/4399465‑4399755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑