| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 334,605 | A→T | 13.0% | L28* (TTG→TAG) ‡ | PP_0276 ← | Cro/CI family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 334,605 | 0 | A | T | 13.0% | 141.8 / 13.7 | 54 | L28* (TTG→TAG) ‡ | PP_0276 | Cro/CI family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (28/19); new base T (4/3); total (32/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01 | |||||||||||
AGCATGTCCATCATGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTC > NC_002947/334458‑334752 | aGCATGTCCATCATGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAg > 1:350426/1‑150 (MQ=255) gCATGTCCATCATGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGt > 2:1254130/1‑150 (MQ=255) gCATGTCCATCATGGCGTCCAAGGTGAATTTATCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGt > 1:387321/1‑150 (MQ=255) atGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTGGAGGCCCCTTTTTTTGATGCAGTCAGTGATGAAAATGGACTTGTCCATTTTCatc < 2:833911/150‑3 (MQ=255) tGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAgg > 2:1920116/1‑150 (MQ=255) gTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCAt > 1:1313639/1‑150 (MQ=255) ggTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCt > 2:1541014/1‑150 (MQ=255) gTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACTTGTCCAttt < 2:1467384/133‑1 (MQ=255) gTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACTTGTCCAttt > 1:1467384/1‑133 (MQ=255) tttCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGt > 1:266732/1‑150 (MQ=255) gATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGc < 1:561297/150‑1 (MQ=255) tCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGcgt > 2:199244/1‑150 (MQ=255) tCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGcgt < 1:1254130/150‑1 (MQ=255) cTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGcgtc < 2:826436/150‑1 (MQ=255) ccATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCgtgg < 2:350426/150‑1 (MQ=255) cATTTGCAAGCTCGGATAGGCGGGCTCGAATGACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCgtggg > 1:990447/1‑150 (MQ=255) tCGGATACGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGaaa < 1:1459650/150‑1 (MQ=255) cGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAg < 1:650737/150‑1 (MQ=255) ggATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGt > 2:1735426/1‑143 (MQ=255) ggATATGCGGGATTGGGTTACGTGCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGt < 1:1735426/143‑1 (MQ=255) ttACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCAGCGTCGTTGGTGGCGTGGAAAGGGCTTTCGTGGAATTGaa < 1:150929/150‑1 (MQ=255) gCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCg > 2:165479/1‑150 (MQ=255) gCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCt > 2:969236/1‑150 (MQ=255) tttGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCt > 1:1836105/1‑150 (MQ=255) tttGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCAATCGGCTTGCCTCGGCAGAAATATCt > 1:1836478/1‑150 (MQ=255) gAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTaaa > 1:1461021/1‑150 (MQ=255) cccATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAAttt > 1:746066/1‑150 (MQ=255) ccATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGTTGGCGTTGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTg > 2:837322/1‑150 (MQ=255) ccATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCTTGGAATTGAAGCGCCATCGGCTTGCCCCGGCAGAAATATTTAAAAtttt > 2:642386/1‑149 (MQ=255) cATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGaaa < 1:626761/91‑1 (MQ=255) cATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGaaa > 2:626761/1‑91 (MQ=255) aTTTTTTTGATGCAGTCAGTGATGAAAATGGGCAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCg > 2:551519/1‑150 (MQ=255) aTTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAgc < 2:237094/111‑1 (MQ=255) aTTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAgc > 1:237094/1‑111 (MQ=255) tttttGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCGCGGCAGAAATATCTAAAATTTGCGATa > 1:1172449/1‑150 (MQ=255) tGATGCAGTCAGTGATGACAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATc < 2:827655/150‑1 (MQ=255) gtGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAt < 2:1836105/150‑1 (MQ=255) gtGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAt < 2:1836478/150‑1 (MQ=255) tgatgaAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAg < 1:1846325/72‑1 (MQ=255) tgatgaAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAg > 2:1846325/1‑72 (MQ=255) gatgaAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGt < 1:1117958/65‑1 (MQ=255) gatgaAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGt > 2:1117958/1‑65 (MQ=255) cttgaAAATGGACTTGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCCGAAatat > 2:1117642/3‑118 (MQ=255) cttgaAAATGGACTTGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAatat < 1:1117642/116‑1 (MQ=255) ttgaAAATGGACTTGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAttgg > 1:1840034/2‑150 (MQ=255) ttgaAAATGGACTTGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAttgg > 2:829660/2‑150 (MQ=255) tgaAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAttggt > 1:1728834/1‑150 (MQ=255) gaAAATGGACAAGGCCATTTTCAAGGCCATTTTGCTGGTGGTATCTGCGTCGTGGGTGGCGTGGAGAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCGTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAttggtt < 2:655723/150‑1 (MQ=255) aaTGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGGAGGAAGGGCTTTTCTTTAAGTTAATCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGc > 1:1178293/1‑150 (MQ=255) gACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGt < 1:199244/150‑1 (MQ=255) gACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGt < 1:1920116/150‑1 (MQ=255) aCAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATATATCGTAGTGTGGATTGGTTGGCGAGTc > 1:870/1‑150 (MQ=255) aCAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAt < 2:183940/137‑1 (MQ=255) aCAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGAt > 1:183940/1‑137 (MQ=255) | AGCATGTCCATCATGGCGTCCAAGGTGAATTTCTCGATCTTGCCATTTGCAAGCTCGGATATGCGGGATTGGGTTACGTTCAGCTTGGCTGCGGCATCATGCTGTTTGAGGCCCATTTTTTTGATGCAGTCAGTGATGAAAATGGACAAGTCCATTTTCAAGGCCATTTTGCTGGCGGTATCTGCGTCGTGGGTGGCGTGGAAAGGGCTTTCGTGGAATTGAAGCGCCATCGGCTTGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTC > NC_002947/334458‑334752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |