Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,419,564 A→C 5.5% E96A (GAG→GCG)  PP_3910 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,419,5640AC 5.5% 208.0 / 4.0 73E96A (GAG→GCG) PP_3910hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/39);  new base C (2/2);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

ATCCCGCAACTACGTCGGCGGTATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTAT  >  NC_002947/4419418‑4419713
                                                                                                                                                  |                                                                                                                                                     
aTCCCGCAACTACGTCGGCGGTATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGaa                                                                                                                                                    <  2:148067/150‑1 (MQ=255)
  cccGCAACTACGTCGGCGGTATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAgg                                                                                                                                                  <  2:917264/150‑1 (MQ=255)
   ccGCAACTACGTCGGCGGTATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCACCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGc                                                                                                                                                 >  1:1199268/1‑150 (MQ=255)
    cGCAACTACGTCGGCGGTATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCt                                                                                                                                                >  1:1851416/1‑150 (MQ=255)
      cAACTACGTCGGCGGTATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGa                                                                                                                                              <  2:1044170/150‑1 (MQ=255)
         cTACGTCGGCGGTATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCa                                                                                                                                           >  1:874984/1‑150 (MQ=255)
          tACGTCGGCGGTATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCAc                                                                                                                                          >  1:1226643/1‑150 (MQ=255)
          tACGTCGGCGGTATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCAc                                                                                                                                          >  1:1226779/1‑150 (MQ=255)
              tCGGCGGTATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAgc                                                                                                                                      <  1:1648371/150‑1 (MQ=255)
                  cggTATCACGCCCAGCGCAGGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCt                                                                                                                                  <  2:874984/150‑1 (MQ=255)
                  cggTATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCt                                                                                                                                  <  1:658514/150‑1 (MQ=255)
                   ggTATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTg                                                                                                                                 <  1:776319/150‑1 (MQ=255)
                     tATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGga                                                                                                                               <  2:860820/150‑1 (MQ=255)
                      aTCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGgac                                                                                                                              <  2:908096/150‑1 (MQ=255)
                         aaGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGCCAGCCGAGAAGGCTGAGTAGCAGCGCCTTgacgac                                                                                                                           <  2:1019516/150‑1 (MQ=255)
                             tCAGCGCCAGCCACGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTATCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACctct                                                                                                                       >  2:863293/1‑150 (MQ=255)
                              cAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTg                                                                                                                      <  1:1744886/150‑1 (MQ=255)
                                 cgcCAGCCACGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTATCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGaa                                                                                                                   <  1:863293/150‑1 (MQ=255)
                                  gcCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGaaa                                                                                                                  <  2:1108879/150‑1 (MQ=255)
                                   ccAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAg                                                                                                                 <  1:715109/150‑1 (MQ=255)
                                    cAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGc                                                                                                                >  1:180644/1‑150 (MQ=255)
                                              cTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTCGAAAGCACACTGcccc                                                                                                      >  2:971767/1‑149 (MQ=255)
                                                 gACGTGCACTGGTGCCGGCCTGACGAATACTGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCCTCGGTACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCATGGACGACCTCTGGAAAGCACACTGCCCGGAc                                                                                                   <  1:119009/150‑1 (MQ=255)
                                                 gACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGAc                                                                                                   <  2:272151/150‑1 (MQ=255)
                                                  aCGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACg                                                                                                  >  1:88397/1‑150 (MQ=255)
                                                     tGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTa                                                                                               <  2:1226779/150‑1 (MQ=255)
                                                     tGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTa                                                                                               <  2:1226643/150‑1 (MQ=255)
                                                      gCACTGGTGCCGGCCTGACGAATACGGCTAACGCCCGCTGAGCAGGATCGCCAAGCATGCCAAGGGCACAAACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTAt                                                                                              >  1:1774326/1‑150 (MQ=255)
                                                        aCTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAgg                                                                                                                                                  <  2:1733145/96‑1 (MQ=255)
                                                        aCTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAgg                                                                                                                                                  >  1:1733145/1‑96 (MQ=255)
                                                          tGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGAc                                                                                          <  2:1876254/150‑1 (MQ=255)
                                                             tGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCgg                                                                                       <  2:1920767/150‑1 (MQ=255)
                                                             tGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCgg                                                                                       <  2:1920740/150‑1 (MQ=255)
                                                              gCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCggg                                                                                      <  2:404713/150‑1 (MQ=255)
                                                               ccGGCCTGATGTATACGGCGACGGCCCGCGGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGa                                                                                     <  2:1851416/150‑1 (MQ=255)
                                                                cGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGAc                                                                                    >  1:142825/1‑150 (MQ=255)
                                                                    cTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACGCCGCCCGGACGCTATCGACCGGGACGaaa                                                                                <  1:51664/150‑1 (MQ=255)
                                                                     tGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAAt                                                                               >  1:1463733/1‑150 (MQ=255)
                                                                     tGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAAt                                                                               >  2:888526/1‑150 (MQ=255)
                                                                      gACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTa                                                                                               <  1:239366/133‑1 (MQ=255)
                                                                      gACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTa                                                                                               >  2:239366/1‑133 (MQ=255)
                                                                        cGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAAtgtg                                                                            <  2:88397/150‑1 (MQ=255)
                                                                         gAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTgg                                                                           >  1:144852/1‑150 (MQ=255)
                                                                           aTACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGa                                                                         >  1:1718139/1‑150 (MQ=255)
                                                                               ggCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGCGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGa                                                                         <  1:1784032/146‑1 (MQ=255)
                                                                               ggCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGCGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGa                                                                         <  1:1784603/146‑1 (MQ=255)
                                                                               ggCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGCGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGa                                                                         >  2:1784032/1‑146 (MQ=255)
                                                                               ggCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGCGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGa                                                                         >  2:1784603/1‑146 (MQ=255)
                                                                                gCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGAAGAACTATAGAAAGAAACCTGAAGAGCGGCGAGTGACGGGGACGAGATGGGGGAGGCCa                                                                    >  2:1153648/1‑150 (MQ=255)
                                                                                   aCCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCg                                                                 <  2:1123223/150‑1 (MQ=255)
                                                                                     cgctcgctGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGAACTCTGGAACTGGCACCGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGc                                                               >  2:989136/1‑150 (MQ=255)
                                                                                        tcgctGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGCGGGGCGTGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTc                                                            >  2:741169/1‑150 (MQ=255)
                                                                                        tcgctGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTc                                                            <  2:1403842/150‑1 (MQ=255)
                                                                                          gctGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGa                                                          >  2:1600462/1‑150 (MQ=255)
                                                                                          gctGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCAAACAGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGa                                                          >  1:1552768/1‑150 (MQ=255)
                                                                                          gctGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGAACTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGa                                                          >  1:28044/1‑150 (MQ=255)
                                                                                               gcCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGTGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTg                                                     >  2:1095324/1‑150 (MQ=255)
                                                                                                        ccAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCctct                                            >  1:508890/1‑150 (MQ=255)
                                                                                                        ccAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCt                                            <  1:194043/150‑1 (MQ=255)
                                                                                                                    aGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCg                                <  1:385565/150‑1 (MQ=255)
                                                                                                                       gCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGAc                                                                                                   >  1:489257/1‑80 (MQ=255)
                                                                                                                       gCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGAc                                                                                                   <  2:489257/80‑1 (MQ=255)
                                                                                                                           aGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGACTTTCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTAtgtg                         >  1:680004/1‑150 (MQ=255)
                                                                                                                            gACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGc                        <  2:1395043/150‑1 (MQ=255)
                                                                                                                             aCAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCt                       >  1:874739/1‑150 (MQ=255)
                                                                                                                                 agagGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAg                   >  2:985479/1‑150 (MQ=255)
                                                                                                                                  gagGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGc                  <  2:144852/150‑1 (MQ=255)
                                                                                                                                     gTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCCCGCCCAGTGGCGGTATGTGCTTCAGCCAc               <  1:989136/150‑1 (MQ=255)
                                                                                                                                        cGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGa                                                                                                    >  2:504566/1‑62 (MQ=255)
                                                                                                                                        cGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGa                                                                                                    <  1:504566/62‑1 (MQ=255)
                                                                                                                                         gggCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCg           <  1:569210/150‑1 (MQ=255)
                                                                                                                                          ggCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGTAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGa          <  1:104585/150‑1 (MQ=255)
                                                                                                                                              gCCGAGAAGGCTGAGACCCAGCGGCTGCTCCTCATCAGGAAAGCTCGCATCCCGAATGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATAcc      <  2:1552768/150‑1 (MQ=255)
                                                                                                                                                 gagaAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGta   <  1:835954/150‑1 (MQ=255)
                                                                                                                                                  agaAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGtat  >  2:420372/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ATCCCGCAACTACGTCGGCGGTATCAAGCTCAGCGCCAGCCGCGACCTGGACGTGCACTGGTGCCGGCCTGACGAATACGGCTACCGCTCGCTGCGCCGGGCGGCCAAGCATGCCAAGGGCACAGACAAAGAGGTGCGGGCAGCCGAGAAGGCTGAGCACCAGCGCCTGGACGACCTCTGGAAAGCACACTGCCCGGACGCTATCGACCGGGACGAAATGTGGGAGGCCATCGGCGTCGAGCGTGGCGGCATCTGGCTCAGTGGCGGTATGTGCTTCAGCCACGGCGATACCGTAT  >  NC_002947/4419418‑4419713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑