Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,425,187 G→C 7.6% intergenic (‑201/‑599) PP_5647 ← / → PP_3917 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,425,1870GC 7.6% 201.5 / 7.7 66intergenic (‑201/‑599)PP_5647/PP_3917hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (30/31);  new base C (2/3);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01

ATCCATTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGG  >  NC_002947/4425038‑4425335
                                                                                                                                                     |                                                                                                                                                    
atCCATTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTTATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCggg                                                                                                                                                      >  1:1293735/1‑150 (MQ=255)
 tCCATTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCgggg                                                                                                                                                     >  1:1318191/1‑150 (MQ=255)
   cagttCGGTTGAAGGCGACCGTTGGAAAATGGTCGGCCCTAATCCTTGCTGATGGCTATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACGGAGCCCGGCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggct                                                                                                                                                   <  2:461229/147‑1 (MQ=255)
    aTTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctg                                                                                                                                                  >  2:396127/1‑150 (MQ=255)
       ccGCTCAAATGCGACCGGTGGAAAAGGATCGGCCCTAATCCTCGCGGATAGCTGTCAGCGGGACAGCGCCAGTAGAGCACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggctgggc                                                                                                                                               <  2:13644/149‑1 (MQ=255)
         gCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCtt                                                                                                                                             >  1:638536/1‑150 (MQ=255)
         gCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCtt                                                                                                                                             >  2:652913/1‑150 (MQ=255)
           ttAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGAAAGATGCGCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCt                                                                                                                                           >  1:1039682/1‑150 (MQ=255)
            tAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAGAAGCCCAGCCCCGCGCTGGGCttttt                                                                                                                                          <  1:1260325/150‑4 (MQ=255)
            tAAAGGCGAACGGTGGAAAATGATCGGACACAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTg                                                                                                                                          <  1:397522/150‑1 (MQ=255)
             aaaTGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGt                                                                                                                                         <  1:396127/150‑1 (MQ=255)
               aTGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTca                                                                                                                                       >  1:1197287/1‑150 (MQ=255)
                tGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTcag                                                                                                                                      <  1:235820/150‑1 (MQ=255)
                  cGACCGGTGGGAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTcagca                                                                                                                                    <  1:1626712/150‑1 (MQ=255)
                  cGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTcagca                                                                                                                                    <  2:1540976/150‑1 (MQ=255)
                  cGACCGGTCGAACACGATCGGACCTAAAACCTGCTGATAGATCTCAGCGAGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTcaaaa                                                                                                                                    <  2:1039682/150‑4 (MQ=255)
                    cccGGTGGAACAAGACCGGCCTCAAACCTCGATGATAGAGAAGATCATGACAGCGCCAGTGGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCaga                                                                                                                                  <  2:208028/149‑1 (MQ=255)
                    aCCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTcagca                                                                                                                                    >  2:498740/1‑148 (MQ=255)
                    aCCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTcagca                                                                                                                                    <  1:498740/148‑1 (MQ=255)
                      cGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGc                                                                                                                                <  2:84833/150‑1 (MQ=255)
                        gTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTc                                                                                                                              <  1:652913/150‑1 (MQ=255)
                             aaaTGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTa                                                                                                                         >  1:870417/1‑150 (MQ=255)
                               aTGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGa                                                                                                                       <  1:413676/150‑1 (MQ=255)
                                   tCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCa                                                                                                                   <  1:617485/150‑1 (MQ=255)
                                   tCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCa                                                                                                                   <  2:653383/150‑1 (MQ=255)
                                     ggCCCTAATCCTTGCTGATAGATAAAAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGc                                                                                                                 <  1:943210/150‑1 (MQ=255)
                                      gCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCg                                                                                                                >  1:846253/1‑150 (MQ=255)
                                              ccTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTa                                                                                                        >  2:812487/1‑150 (MQ=255)
                                                ttGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCACTTACg                                                                                                      >  1:53460/1‑150 (MQ=255)
                                                ttGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACg                                                                                                      >  2:516581/1‑150 (MQ=255)
                                                   cTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCaa                                                                                                           <  2:358081/142‑1 (MQ=255)
                                                   cTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCaa                                                                                                           >  1:358081/1‑142 (MQ=255)
                                                      atagataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggct                                                                                                                                                   <  1:1530775/99‑1 (MQ=255)
                                                      atagataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGgggct                                                                                                                                                   >  2:1530775/1‑99 (MQ=255)
                                                       tagataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCa                                                                                               <  1:1491829/150‑1 (MQ=255)
                                                         gataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCt                                                                                                                                           >  2:1526010/1‑104 (MQ=255)
                                                         gataTCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCt                                                                                                                                           <  1:1526010/104‑1 (MQ=255)
                                                             tCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTca                                                                                                                                       <  1:1336517/104‑1 (MQ=255)
                                                             tCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTca                                                                                                                                       >  2:1336517/1‑104 (MQ=255)
                                                             tCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACt                                                                                         >  2:690739/1‑150 (MQ=255)
                                                               aGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCa                                                                                                            >  1:275332/1‑129 (MQ=255)
                                                               aGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCa                                                                                                            <  2:275332/129‑1 (MQ=255)
                                                                                      ccGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCtata                                                                <  1:1044627/150‑1 (MQ=255)
                                                                                      ccGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCtata                                                                <  2:1393306/150‑1 (MQ=255)
                                                                                              gATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGAt                                                                       <  1:1092418/135‑1 (MQ=255)
                                                                                              gATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCgg                                                        >  2:1708485/1‑150 (MQ=255)
                                                                                              gATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGGCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGAt                                                                       >  2:1092418/1‑135 (MQ=255)
                                                                                                    tGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAg                                                                     >  1:1881286/1‑131 (MQ=255)
                                                                                                    tGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAg                                                                     <  2:1881286/131‑1 (MQ=255)
                                                                                                     gAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGa                                                 <  1:1708485/150‑1 (MQ=255)
                                                                                                       gCCCGTCCAGCAGACAGAAGTTCAGGTAGCAAAAGGCCCAGCGCGGGGCTCGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCGCGGCATTGGATg                                               >  2:1057823/1‑150 (MQ=255)
                                                                                                       gCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATg                                               >  2:837973/1‑150 (MQ=255)
                                                                                                         ccGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGcc                                             >  2:812461/1‑150 (MQ=255)
                                                                                                           gTCCAGCATACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCt                                           >  2:1508559/1‑150 (MQ=255)
                                                                                                               agcagACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGAt                                       >  1:904938/1‑150 (MQ=255)
                                                                                                                 cagacagaAGTTCAAGTAGCAAAACGCACAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATgg                                     <  2:240181/150‑1 (MQ=255)
                                                                                                                 cagacagaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATgg                                     <  2:1293735/150‑1 (MQ=255)
                                                                                                                      agaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCg                                                                                                                <  2:1142204/70‑1 (MQ=255)
                                                                                                                      agaAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCg                                                                                                                >  1:1142204/1‑70 (MQ=255)
                                                                                                                      agaAGTTAAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCg                                >  2:1860388/1‑150 (MQ=255)
                                                                                                                        aaGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGt                              <  2:1384067/150‑1 (MQ=255)
                                                                                                                        aaGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGt                              >  1:1299757/1‑150 (MQ=255)
                                                                                                                          gTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTCGCAGATATTCTAAAGGGTGCTTGTGATATCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTgg                            >  1:224393/1‑150 (MQ=38)
                                                                                                                           ttCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTggg                           >  1:72872/1‑150 (MQ=255)
                                                                                                                              aaGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTAGCAGGTGCTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCt                        >  2:913309/1‑150 (MQ=255)
                                                                                                                                  aGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGtt                    <  1:690739/150‑1 (MQ=255)
                                                                                                                                  aGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGtt                    <  1:1149179/150‑1 (MQ=255)
                                                                                                                                    acagaaGCCCAGCCCCGCGCTGGGCTTTTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCtt                       <  1:843799/141‑1 (MQ=255)
                                                                                                                                    acagaaGCCCAGCCCCGCGCTGGGCTTTTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCtt                       >  2:843799/5‑145 (MQ=255)
                                                                                                                                       gaaGCCCAGCCCCGCGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTtg                                                                           <  1:412225/89‑1 (MQ=39)
                                                                                                                                       gaaGCCCAGCCCCGCGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTtg                                                                           >  2:412225/2‑90 (MQ=39)
                                                                                                                                            ccAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGcc          >  1:294199/1‑150 (MQ=255)
                                                                                                                                                 gcgGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGaa     <  2:1299757/150‑1 (MQ=255)
                                                                                                                                                    gggctgggctTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAgg  <  1:59237/150‑1 (MQ=255)
                                                                                                                                                    gggctgggctTCTATCAGCAGAGCTCGACTAGACCCAGCTGTCTATTAGGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAgg  <  2:682531/150‑1 (MQ=37)
                                                                                                                                                     |                                                                                                                                                    
ATCCATTTCGCTTAAATGCGACCGGTGGAAAATGATCGGCCCTAATCCTTGCTGATAGATATCAGCGGGACAGCGCCAGTAGAGAACCGAGCCCGATCACTGAGCCCGTCCAGCAGACAGAAGTTCAAGTAGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTGTCAGCAGAGCTCGACTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGG  >  NC_002947/4425038‑4425335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑