Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,441,977 C→A 6.3% A200S (GCG→TCG)  nicP‑II ← porin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,441,9770CA 6.3% 250.1 / 3.1 80A200S (GCG→TCG) nicP‑IIporin‑like protein
Reads supporting (aligned to +/- strand):  ref base C (37/37);  new base A (2/3);  total (40/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

GCGCTGCTGGTAGATATCCTCCAACTGCGCCTGCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCTGAACAGCAGCACC  >  NC_002947/4441828‑4442125
                                                                                                                                                     |                                                                                                                                                      
gcgcTGCTGGTAGATATCCTCCAACTGCGCCTGCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGATCGCCGGGAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAgcgc                                                                                                                                                        >  1:1367477/1‑150 (MQ=255)
         gTAGATATCCTCCAACTGCGCCTGCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACAtct‑t                                                                                                                                              >  1:722716/1‑150 (MQ=255)
         gTAGATATCCTCCAACTGCGCCTGCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACAtct‑t                                                                                                                                              >  1:1412005/1‑150 (MQ=255)
          tAGATATCCTCCAACTGCGCCTGCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACAtct‑tc                                                                                                                                             >  1:712304/1‑150 (MQ=255)
          tAGATATCCTCCAACTGCGCCTGCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACAtct‑tc                                                                                                                                             <  2:57837/150‑1 (MQ=255)
                  ctccAACTGCGCCTGCCAGACACCGACGCTGGTACGGATGTCGGTTGAGGCAGATTCGTCACCCACGGATTTTAACCGGTCGCTGTTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACAAGCGCGTACATCT‑TCTGG‑CTGtt                                                                                                                                    >  1:1571464/1‑150 (MQ=255)
                     cAACTGCGCCTGCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGa                                                                                                                                 <  1:1522751/150‑1 (MQ=255)
                      aaCTGCGCCTGCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGAc                                                                                                                                <  1:1209798/150‑1 (MQ=255)
                        cTGCGCCTGCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGa                                                                                                                              <  2:452647/150‑1 (MQ=255)
                            gcCTGCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTcg                                                                                                                          >  2:1087950/1‑150 (MQ=255)
                               tGCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCCCCCACGTAGTTGAACCGGTCGCTGGTAAAAAGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCgcgc                                                                                                                       >  1:1295171/1‑150 (MQ=255)
                               tGCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCgcgc                                                                                                                       >  2:1500838/1‑150 (MQ=255)
                                gCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCa                                                                                                                      <  2:1571464/150‑1 (MQ=255)
                                  cAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAgg                                                                                                                    >  2:363084/1‑150 (MQ=255)
                                  cAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAgg                                                                                                                    >  2:1875463/1‑150 (MQ=255)
                                  cAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGGCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAgg                                                                                                                    >  2:1875752/1‑150 (MQ=255)
                                   aGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGGTCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGc                                                                                                                   >  1:80657/1‑150 (MQ=255)
                                   aGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGCGGGTCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGc                                                                                                                   >  1:1262009/1‑150 (MQ=255)
                                     acacCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCtg                                                                                                                 <  1:606412/150‑1 (MQ=255)
                                     acacCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCtg                                                                                                                 <  2:722716/150‑1 (MQ=255)
                                       acCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCtggt                                                                                                               >  2:1631712/1‑150 (MQ=255)
                                       acCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCtggt                                                                                                               >  1:1682972/1‑150 (MQ=255)
                                          gACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGa                                                                                                            >  2:12222/1‑150 (MQ=255)
                                            cGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGc                                                                                                          <  2:920186/150‑1 (MQ=255)
                                             gCTGGTCCGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCATCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCg                                                                                                         <  1:327929/150‑1 (MQ=255)
                                               tGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGa                                                                                                       >  1:933583/1‑150 (MQ=255)
                                                 gTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAAc                                                                                                     >  1:1536255/1‑150 (MQ=255)
                                                     ggTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACACCT‑CCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGc                                                                                                 <  2:1551663/150‑1 (MQ=255)
                                                      gTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGcc                                                                                                >  2:1241671/1‑150 (MQ=255)
                                                       ttGTCGTTGAAGGCATAGTCGGCACCCACGTCGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCGGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCCGGCTCGTGGCGCGGAACTGGCCg                                                                                               >  2:304022/1‑150 (MQ=255)
                                                           cGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGgcgc                                                                                           <  2:1536255/150‑1 (MQ=255)
                                                              tGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCa                                                                                        <  1:1230983/150‑1 (MQ=255)
                                                                    cATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCa                                                                                  >  2:1392304/1‑150 (MQ=255)
                                                                     aTAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAAAGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCaa                                                                                 <  2:1295171/150‑1 (MQ=255)
                                                                         tCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGt                                                                                    <  2:1795449/143‑1 (MQ=255)
                                                                         tCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGt                                                                                    >  1:1795449/1‑143 (MQ=255)
                                                                           ggCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGc                                                                           <  1:1087950/150‑1 (MQ=255)
                                                                           ggCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGAGCGTAGATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGc                                                                           <  1:1564078/150‑1 (MQ=255)
                                                                                ccACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACt                                                                      >  2:1770118/1‑150 (MQ=255)
                                                                                 cACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTc                                                                     >  1:243203/1‑150 (MQ=255)
                                                                                           aaCCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTcgc                                                                   <  1:344999/142‑1 (MQ=255)
                                                                                           aaCCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTcgc                                                                   >  2:344999/1‑142 (MQ=255)
                                                                                                gTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCgg                                                                            <  1:1353989/128‑1 (MQ=255)
                                                                                                gTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCgg                                                                            >  2:1353989/1‑128 (MQ=255)
                                                                                                gTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCAt                                                      <  2:1317458/150‑1 (MQ=255)
                                                                                                gTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCAt                                                      >  2:751994/1‑150 (MQ=255)
                                                                                                 tCGCTGGTAACACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACGTCT‑TCTAT‑CTGTTGGACGAGACGCGCAGGCTTGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATc                                                     <  1:1875463/150‑1 (MQ=255)
                                                                                                 tCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCCGAGCGTATGTCTATTTCT‑AAGAAGG‑CGAGTCCAGCAGGCTTGTCGACCAGAACCGGCCGGCCCCGAGGTTCAACCCGGCGAACTCGCGCGACTCCAGCATc                                                     <  1:1875752/150‑1 (MQ=255)
                                                                                                 tCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTTGTTGAGTGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATc                                                     <  2:1412005/150‑1 (MQ=255)
                                                                                                     tGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGAGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCgcgc                                                 >  1:1896485/1‑150 (MQ=255)
                                                                                                     tGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGAGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCgcgc                                                 >  1:1897073/1‑150 (MQ=255)
                                                                                                     gggTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCgcgc                                                 >  2:747270/2‑150 (MQ=255)
                                                                                                         cctgagcgcCAGCCGCGCCGGGTTGGTCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGGGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTg                                             >  2:382553/6‑150 (MQ=255)
                                                                                                         aaaaCGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGCCTCCAGCATCGCGCCCTg                                             <  2:933583/150‑1 (MQ=255)
                                                                                                           aaCGCGCCAGCCGCGCCGGGTTGATCGGGTCGCTGACAACCCCGTACATCT‑TCTAA‑CGGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGCCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGt                                           <  1:782379/150‑1 (MQ=255)
                                                                                                               cgcCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTCTTGAACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGt                                       <  1:498643/150‑1 (MQ=255)
                                                                                                                 ccAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGGGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGGGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTcg                                     >  1:628215/1‑150 (MQ=255)
                                                                                                                 ccAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGt                                           >  1:1300927/1‑144 (MQ=255)
                                                                                                                 ccAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGt                                           <  2:1300927/144‑1 (MQ=255)
                                                                                                                  cAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTcgg                                    >  1:1230073/1‑150 (MQ=255)
                                                                                                                   aGCCGCGCCGGGTTGCTCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTcggc                                   <  1:420286/150‑1 (MQ=255)
                                                                                                                   aGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTcggc                                   >  1:641497/1‑150 (MQ=255)
                                                                                                                   aGCCGCGCCGGGTTGATCGGGTCGTTGAACAGCGAGT‑TTGCT‑TTTGGTCTGTTTGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTcggc                                   <  1:423910/150‑1 (MQ=255)
                                                                                                                    gCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTcggcg                                  <  1:1392304/150‑1 (MQ=255)
                                                                                                                     ccGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTcggcgg                                 <  1:586408/150‑1 (MQ=255)
                                                                                                                      cgcgCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCggcggc                                <  2:1262009/150‑1 (MQ=255)
                                                                                                                           cGGGTTGATCAGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGcagcag                           >  1:695819/1‑150 (MQ=255)
                                                                                                                                   tCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCaacg                   >  1:967359/1‑148 (MQ=255)
                                                                                                                                   tCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAAt                   >  2:262272/1‑150 (MQ=255)
                                                                                                                                   tCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAAt                   >  2:1585552/1‑150 (MQ=255)
                                                                                                                                       gTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCt               <  1:272098/150‑1 (MQ=255)
                                                                                                                                       gTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCt               <  1:1770118/150‑1 (MQ=255)
                                                                                                                                       gTCGTTGACCAGCGAGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCt               <  2:1896485/150‑1 (MQ=255)
                                                                                                                                       gTCGTTGACCAGCGAGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCt               <  2:1897073/150‑1 (MQ=255)
                                                                                                                                          gTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCTGaa            <  2:818173/150‑1 (MQ=255)
                                                                                                                                          gTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCTGaa            <  1:1241671/150‑1 (MQ=255)
                                                                                                                                           ttGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCTGAAc           >  2:733096/1‑150 (MQ=255)
                                                                                                                                              aCCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCTGAAcagc        <  2:80657/150‑1 (MQ=255)
                                                                                                                                               ccAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCTGAAcagca       <  2:1682972/150‑1 (MQ=255)
                                                                                                                                               ccAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCTGAAcagca       <  2:695819/150‑1 (MQ=255)
                                                                                                                                                cAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCTGAAcagcag      >  2:1830872/1‑150 (MQ=255)
                                                                                                                                                    gcgTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCTGAACAGCAGCAcc  <  1:717388/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GCGCTGCTGGTAGATATCCTCCAACTGCGCCTGCCAGACACCGACGCTGGTACGGTTGTCGTTGAAGGCATAGTCGGCACCCACGTAGTTGAACCGGTCGCTGGTAAAACGCGCCAGCCGCGCCGGGTTGATCGGGTCGTTGACCAGCGCGTACATCT‑TCTGG‑CTGTTGGACGAGTCGCGCAGGCTGGTGGAGCGGAACTGGCCGGCGCTCAGGGTCAACCCGGCGAACTCGCGCGACTCCAGCATCGCGCCCTGGTATGTCGGCGGCAGCAGGCGCAAATCGCTGAACAGCAGCACC  >  NC_002947/4441828‑4442125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑