| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,447,887 | C→G | 10.5% | intergenic (‑279/‑43) | nicC ← / → nicX | 6‑hydroxynicotinate 3‑monooxygenase/2,5‑dihydroxypyridine 5,6‑dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,447,887 | 0 | C | G | 10.5% | 190.6 / 8.4 | 64 | intergenic (‑279/‑43) | nicC/nicX | 6‑hydroxynicotinate 3‑monooxygenase/2,5‑dihydroxypyridine 5,6‑dioxygenase |
| Reads supporting (aligned to +/- strand): ref base C (25/32); new base G (4/3); total (29/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCCGGTTGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGCCACTACTCCG > NC_002947/4447738‑4448035 | aaTCCGGTTGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCAc < 1:1602764/150‑1 (MQ=255) gATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGt < 2:1618855/150‑1 (MQ=255) gATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGt < 2:1101571/150‑1 (MQ=255) tGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCATGGCAGTGCC‑TGGTgct < 1:559237/150‑2 (MQ=255) tGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTgcg > 1:1877613/1‑150 (MQ=255) gTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCATGGCAGTGCC‑TGGTgctg < 1:84310/150‑3 (MQ=255) gTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGt < 2:1394543/150‑1 (MQ=255) aaTACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCaa < 1:867496/150‑1 (MQ=255) aTACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAAc < 1:9201/150‑1 (MQ=255) aCTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGc < 1:1567439/150‑1 (MQ=255) tGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCa < 1:464279/150‑1 (MQ=255) tgtgtgGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGga < 2:79971/150‑1 (MQ=255) tgtgtgGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCACCACCA‑GGCACTGCCATGGTGCTCCCCTGTCATCGCTACCCAGga < 2:1755865/150‑1 (MQ=255) gtgtgGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGgag < 2:418792/150‑1 (MQ=255) gTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAAc > 1:1622595/1‑128 (MQ=255) gTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAAc < 2:1622595/128‑1 (MQ=255) gTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATg > 1:155682/1‑150 (MQ=255) aCGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCg < 2:291029/150‑1 (MQ=255) aCGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCg < 2:155682/150‑1 (MQ=255) gTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGt > 2:1320324/1‑150 (MQ=255) ttGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGa < 2:1436796/150‑1 (MQ=255) tGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAg < 1:21692/150‑1 (MQ=255) aTCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGca > 1:202598/1‑150 (MQ=255) tCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGcac > 2:1416351/1‑150 (MQ=255) aCTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACaa < 2:1877613/150‑1 (MQ=255) aCTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACaa < 2:202598/150‑1 (MQ=255) tGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGCGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGa > 1:1411043/1‑118 (MQ=255) tGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGCGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGa < 2:1411043/118‑1 (MQ=255) cAGGATGCGTTTTGATCTGCACAAGTCGGGTGTACACATTATTATTCTGCACCGGGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCCATGCACAACTGACCCAGATGTTc < 2:815628/150‑1 (MQ=255) cAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACt < 1:484442/73‑1 (MQ=255) cAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACt > 2:484442/1‑73 (MQ=255) cAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTc > 2:974104/1‑150 (MQ=255) gATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAg < 1:1888817/150‑1 (MQ=255) tCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGcc > 2:1255312/1‑64 (MQ=255) tCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGcc < 1:1255269/64‑1 (MQ=255) tCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGcc > 2:1255269/1‑64 (MQ=255) tCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGcc < 1:1255312/64‑1 (MQ=255) tCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGcc < 1:1255488/64‑1 (MQ=255) tCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGcc > 2:1255488/1‑64 (MQ=255) aTCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGTACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGa < 1:441531/110‑1 (MQ=255) aTCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGTACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGa > 2:441531/1‑110 (MQ=255) tCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAg > 2:934159/1‑150 (MQ=255) tCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCAAAACTGACCCAGATGTTCGAGCACGTACTGAAg > 2:1447459/1‑150 (MQ=255) acaAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGAGCCAGATGTTCGAGCACGTACTGAAGCTGtc > 2:1604331/1‑150 (MQ=255) aGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACACCTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGt < 1:240550/150‑1 (MQ=255) cacacTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGa < 2:575946/90‑1 (MQ=255) cacacTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGa > 1:575946/1‑90 (MQ=255) acTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAgg > 2:201039/1‑68 (MQ=255) acTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAgg < 1:201039/68‑1 (MQ=255) cTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAGCCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCa < 1:974104/150‑1 (MQ=255) cTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCa < 1:1320324/150‑1 (MQ=255) cTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCAACCACGAGTCCCATGGTATCGGTGAGCAATGGTGTTCTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCa < 1:1178322/150‑1 (MQ=255) tttGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTATTGAAGCTGTCTCGAGTGGACGAAAAGCAGAGCGTg < 2:1014065/150‑1 (MQ=255) gCACCGAGATGCAGCACCA‑GCCAGTGCCATGGTGCGCCCCTGTCAACGCCACCCAAGAAAACAACATTGCCGTTGAGCAATGCTCAACTGACACAGATGGTCGAGCACGTACTGATGCTGTCTCCAGTTGACGAAACGCAGAGCGTGGcc < 2:826758/150‑1 (MQ=255) aCCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGt > 1:251281/1‑150 (MQ=255) ccGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAGGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTg > 2:40547/1‑150 (MQ=255) cGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGc > 1:860371/1‑150 (MQ=255) cGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACGGAAGCTGTCTCGAGAGGACGAAACGCAGAGCGTGGCCGtcc > 2:36335/1‑148 (MQ=255) ggggcgcaCCATGGCAGTGCC‑TGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTAGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCaa > 2:916604/6‑150 (MQ=255) gggcgcaCCATGGCAGTGCC‑TGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCaaa > 2:1728328/5‑149 (MQ=255) gcaCCATGGCAGTGCC‑TGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGcc > 2:1790979/1‑150 (MQ=255) gcaCCATGGCAGTGCC‑TGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGcc > 2:621720/1‑150 (MQ=255) ggCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGCCactacc > 2:1509747/1‑149 (MQ=255) aCTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGCCACTACTCCg > 1:1645407/1‑150 (MQ=255) aCTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGCCACTACTCCg > 1:1645214/1‑150 (MQ=255) | AATCCGGTTGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCGATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGCCACTACTCCG > NC_002947/4447738‑4448035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |