| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,479,221 | G→C | 10.6% | W176C (TGG→TGC) | ybdR → | Zn‑dependent oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,479,221 | 0 | G | C | 10.6% | 168.8 / 11.3 | 58 | W176C (TGG→TGC) | ybdR | Zn‑dependent oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (29/22); new base C (3/3); total (33/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 | |||||||||||
TTCGGATACAGCCATCTGTATGGAGGGATCCCTGGAGGCCAAGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACACCGTCTTCATGGTGGATGACAACGACTACCGGCTGGCCTA > NC_002947/4479072‑4479370 | ttCGGATACAGCCATCTGTATGGAGGGATCCCTGGAGGCCAAGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTgg > 2:901055/1‑150 (MQ=255) gATACAGCCAACTGTATGGAGGGATCCCTGGAGGCCAAGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGACAAg > 2:381365/1‑150 (MQ=255) aCAGCCATCTGTATGGAGGGATCCCTGGAGGCCAAGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAGAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCgg < 1:703744/150‑1 (MQ=255) gCCATCTGTATGGAGGGATCCCTGGAGGCCAAGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGtca < 2:473818/150‑1 (MQ=255) cATCTGTATGGAGGGATCCCTGGAGGCCACGCTGATTACTTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGtcatc < 2:150896/150‑1 (MQ=255) aTCTGTATGGAGGGATCCCTGGAGGCCAAGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGCCAAGCGGtgggaa < 2:1304312/150‑6 (MQ=255) tCTGTATGGAGGGATCCCTGGAGGCCAAGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCaa > 1:1667046/1‑150 (MQ=255) tGTATGGAGGGATCCCTGGAGGCCAAGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATg < 2:537147/150‑1 (MQ=255) tATGGAGGGATCCCTGGAGGCCAAGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGcc < 1:1210075/150‑1 (MQ=255) tGGAGGGATCCCTGGAGGCCAAGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCga < 1:901055/150‑1 (MQ=255) ccTGGAGGCCAAGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGaa < 2:1149098/150‑1 (MQ=255) ccTGGAGGCCAAGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGaa < 2:1149365/150‑1 (MQ=255) aaGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCgg > 2:560848/1‑150 (MQ=255) aGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGt > 2:1146503/1‑150 (MQ=255) gCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGCCAAGCGGtgg < 1:306654/118‑3 (MQ=255) gCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGCCAAGCGGtgg > 2:306654/1‑116 (MQ=255) gTTAGGGTGCCCCTAGGCAATGTCGGGCCATTCAAAGTCACGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTAt > 2:648097/1‑150 (MQ=255) gTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGa > 1:1396130/1‑145 (MQ=255) gTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGa < 2:1396130/145‑1 (MQ=255) gTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTAt > 1:1666859/1‑150 (MQ=255) ttAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCTGAGATTGGCGAAGGCTCATCGGTAGCGATCTATg < 1:626613/150‑1 (MQ=255) gTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGcgcg > 1:670312/1‑150 (MQ=255) cAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGc < 1:1625992/115‑1 (MQ=255) cAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGc > 2:1625992/1‑115 (MQ=255) aaaGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCa > 2:1916781/1‑150 (MQ=255) ggCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTg > 1:500505/1‑150 (MQ=255) aaTGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTgggg > 1:216967/1‑150 (MQ=255) aaTGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTgggg > 1:504859/1‑150 (MQ=255) aaTGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGCGCCAGTtggg < 2:1013554/150‑1 (MQ=255) cGGGCCATTCAAAGTCCCGACGATCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGtt < 2:514924/150‑1 (MQ=255) gggCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTg > 1:282634/1‑150 (MQ=255) ggCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCATGAAGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGa > 1:1772407/1‑150 (MQ=255) gCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAg > 1:325251/1‑150 (MQ=255) ccATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGt < 2:1243144/150‑1 (MQ=255) tCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTgccg > 2:1727910/1‑150 (MQ=255) gacgacCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGCTTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACg > 1:511681/1‑150 (MQ=255) acgacCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACgg > 2:1880662/1‑150 (MQ=255) gcgGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGgc < 1:1511798/150‑1 (MQ=255) gcgGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGgc < 1:560848/150‑1 (MQ=255) ggATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCggg > 2:306910/1‑105 (MQ=255) ggATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCggg < 1:306910/105‑1 (MQ=255) aaaGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACAcc > 1:857404/1‑150 (MQ=255) cTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACg < 2:752498/125‑1 (MQ=255) cTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACg > 1:752498/1‑125 (MQ=255) gTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGACGCTGGGCGCGCACACCGTCTTCAt < 1:1880662/150‑1 (MQ=255) cTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACACCGTCTTCAtggtg > 2:836172/1‑150 (MQ=255) aTCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACACCGTCTTCATGGTGGa > 1:983325/1‑150 (MQ=255) gACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCCCACCGTCTTCATGGTGGATGAc > 2:1080184/1‑150 (MQ=255) gACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACACCGTCTTCATGGTGGATGAc > 2:64370/1‑150 (MQ=255) cTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGtt > 2:929593/1‑89 (MQ=255) cTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGtt < 1:929593/89‑1 (MQ=255) ttCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACACCGTCTTCATGGTGGATGACAACGACt > 1:1211577/1‑150 (MQ=255) atgaCCGCTTGCCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACACCGTCTTCATGGTGGATGACAACGACTAc > 1:372416/4‑150 (MQ=255) ccACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACACCGTCTTCATGGTGGATGACAACGACTAcc > 2:165387/1‑150 (MQ=255) gaCCGCTTGCCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGtt < 2:1818235/83‑1 (MQ=255) gaCCGCTTGCCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGtt > 1:1818235/2‑84 (MQ=255) cGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACACCGTCTTCATGGTGGATGACAACGACTACCGGCt < 1:165387/150‑1 (MQ=255) cGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACACCGTCTTCATGGTGGATGACAACGACTACCGGCt > 2:932828/1‑150 (MQ=255) ttGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACACCGTCTTCATGGTGGATGACAACGACTACCGGCTGGc < 2:158302/150‑1 (MQ=255) gCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACACCGTCTTCATGGTGGATGACAACGACTACCGGCTGGCCTa > 2:1670406/1‑150 (MQ=255) gCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACACCGTCTTCATGGTGGATGACAACGACTACCGGCTGGCCTa > 2:1116145/1‑150 (MQ=255) | TTCGGATACAGCCATCTGTATGGAGGGATCCCTGGAGGCCAAGCTGATTACGTTAGGGTGCCCAAAGGCAATGTCGGGCCATTCAAAGTCCCGACGACCCTCGCGGATGACAAAGTGCTGTTCCTATCCGACATCCTTCCCACCGCTTGGCAAGCGGTCATCAATGCCGAGATTGGCGAAGGCTCATCGGTAGCGATCTATGGCGCGGGGCCAGTGGGGCTGTTGAGTGCCGCCTGCGCACGGATGCTGGGCGCGCACACCGTCTTCATGGTGGATGACAACGACTACCGGCTGGCCTA > NC_002947/4479072‑4479370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |