Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,535,346 T→C 6.5% F207F (TTT→TTC)  PP_4022 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,535,3460TC 6.5% 206.9 / 2.2 70F207F (TTT→TTC) PP_4022hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/41);  new base C (2/3);  total (26/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CATTGCCGAGAACAGTGTTATTAAAAAAGAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTATCATCAGCTTCATTATCACCCGCTACAAAAAACAAATTAAC  >  NC_002947/4535199‑4535495
                                                                                                                                                   |                                                                                                                                                     
cATTGCCGAGAACAGTGTTATTAAAAAAGAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTga                                                                                                                                                     <  2:156065/150‑1 (MQ=255)
  ttGCCGAGAACAGTGTTATTAAAAAAGAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTgaga                                                                                                                                                   <  2:604717/150‑1 (MQ=255)
   tGCCGAGAACAGTGTTATTAAAAAAGAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGagag                                                                                                                                                  <  2:1086003/150‑1 (MQ=255)
     ccGAGAACAGTGTTATTAAAAAAGAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAACGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCt                                                                                                                                                <  2:1492858/150‑1 (MQ=255)
       gagaACAGTGTTATTAAAAAAGAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTa                                                                                                                                              <  2:1780448/150‑1 (MQ=255)
        agaACAGTGTTATTAAAAAAGAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGATAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACTAGCTAATGGACGACTTTGAGAGCTTAc                                                                                                                                             <  1:355439/150‑1 (MQ=255)
        agaACAGTGTTATTAAAAAAGAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTAc                                                                                                                                             <  2:316790/150‑1 (MQ=255)
              gtgtTATTAAAAAAGAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTc                                                                                                                                       <  2:1738328/150‑1 (MQ=255)
                   attaAAAAAGAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATc                                                                                                                                  <  2:1272672/150‑1 (MQ=255)
                       aaaaaGAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTgg                                                                                                                              >  2:57432/1‑150 (MQ=255)
                        aaaaGAACTAAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGATAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTgga                                                                                                                             <  1:968041/150‑1 (MQ=255)
                            gAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTga                                                                                                                                                     >  1:1074593/1‑122 (MQ=255)
                            gAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTga                                                                                                                                                     <  2:1074593/122‑1 (MQ=255)
                               cTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGaa                                                                                                                      <  2:1139323/150‑1 (MQ=255)
                                    aaCCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGngat                                                                                                                 <  2:1204525/150‑1 (MQ=255)
                                     aCCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGtgatg                                                                                                                >  1:667569/1‑150 (MQ=255)
                                           tCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAAt                                                                                                          <  1:1474155/150‑1 (MQ=255)
                                           tCTGACACAGGTAAAGCCATGAACCAATTCACCGACTTAATGGAGAACAACCTTATCAGCATAGACAAACAGGGACACCTGAAGTACAAGCTAATGGACTTCATCTATATCTTACGATTGCTAATCCTGGAGGACGAAGTGATGAAAAAt                                                                                                          <  1:1774559/150‑1 (MQ=255)
                                             tGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGa                                                                                                        <  1:1051290/150‑1 (MQ=255)
                                               acacAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGAcc                                                                                                      <  1:367695/150‑2 (MQ=255)
                                               acacAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACa                                                                                                      >  1:934384/1‑150 (MQ=255)
                                                   aGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTAAGCTTCCAAATCCTTCTGGATGATGTGATTAAAAATGACAAGAc                                                                                                  >  2:495425/1‑150 (MQ=255)
                                                            ccTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAATACATTGAACAg                                                                                         >  1:121741/1‑150 (MQ=255)
                                                            ccTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTCGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAg                                                                                         >  2:80107/1‑150 (MQ=255)
                                                             cTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGGCAACTGAAGTACAACCTAATGGACGACTTTGAGAGCTTACGATGTCAAATCCTGGAGGTCGCAGTGATGAAAAACGACGAGACAGCGAACAGt                                                                                        >  2:985450/1‑150 (MQ=255)
                                                             cTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGt                                                                                        >  2:198875/1‑150 (MQ=255)
                                                             cTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGt                                                                                        <  2:1756732/150‑1 (MQ=255)
                                                              tGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTc                                                                                       <  2:1685826/150‑1 (MQ=255)
                                                                aaCCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTa                                                                                                                                              >  2:1422564/1‑93 (MQ=255)
                                                                aaCCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTa                                                                                                                                              <  1:1422564/93‑1 (MQ=255)
                                                                  ccAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCtttt                                                                                   <  1:1816877/150‑1 (MQ=255)
                                                                  ccAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCtttt                                                                                   <  2:1442953/150‑1 (MQ=255)
                                                                  ccAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGAACTTGAAGACTCttaa                                                                                   <  2:934384/150‑3 (MQ=255)
                                                                    aaTTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGt                                                                                 <  2:922530/150‑1 (MQ=255)
                                                                     aTTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTc                                                                                >  1:1414519/1‑150 (MQ=255)
                                                                     aTTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTCGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGGAAAATGACAAGACATTGAACAGTCTTTTGTc                                                                                >  2:1621209/1‑150 (MQ=255)
                                                                        cACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGc                                                                             <  1:944072/150‑1 (MQ=255)
                                                                        cACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGc                                                                             <  1:1637323/150‑1 (MQ=255)
                                                                         aCCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCa                                                                            <  2:621692/150‑1 (MQ=255)
                                                                                    ggAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGc                                                                 <  1:1140279/150‑1 (MQ=255)
                                                                                     gagaACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCtt                                                                                     >  2:1880398/1‑129 (MQ=255)
                                                                                     gagaACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCtt                                                                                     <  1:1880398/129‑1 (MQ=255)
                                                                                       gaACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCaa                                                              <  1:198875/150‑1 (MQ=255)
                                                                                           aaaCTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACAt                                                                                                >  2:815940/1‑112 (MQ=255)
                                                                                           aaaCTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACAt                                                                                                <  1:815940/112‑1 (MQ=255)
                                                                                           aaaCTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGaa                                                          >  2:1143429/1‑150 (MQ=255)
                                                                                           aaaCTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGaa                                                          >  1:1053210/1‑150 (MQ=255)
                                                                                               ttATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTg                                                      >  1:1357792/1‑150 (MQ=255)
                                                                                                 aTCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGaa                                                    <  2:655257/150‑1 (MQ=255)
                                                                                                       aTAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTCGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGGAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGt                                              <  1:1621209/150‑1 (MQ=255)
                                                                                                         aGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCt                                                       <  1:1497744/139‑1 (MQ=255)
                                                                                                         aGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCt                                                       >  2:1497744/1‑139 (MQ=255)
                                                                                                            cAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTatc                                         <  1:1792805/150‑1 (MQ=255)
                                                                                                             aaaCAGGGACAACTGAAGTACAAGCTAATGGACGACTTCGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTatca                                        <  1:80107/150‑1 (MQ=255)
                                                                                                               aCAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAAt                                                                                                          <  1:249812/82‑1 (MQ=255)
                                                                                                               aCAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAAt                                                                                                          >  2:249812/1‑82 (MQ=255)
                                                                                                               aCAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTatcatc                                      >  1:25294/1‑150 (MQ=255)
                                                                                                               aCAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTatcatc                                      <  2:1414519/150‑1 (MQ=255)
                                                                                                                 aGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTATCATCAg                                    <  1:346075/150‑1 (MQ=255)
                                                                                                                   ggACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGaaaaa                                                                                                           >  2:1855002/1‑77 (MQ=255)
                                                                                                                   ggACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGaaaaa                                                                                                           <  1:1855002/77‑1 (MQ=255)
                                                                                                                       aaCTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTATCATCAGCTTCAt                              >  1:1887585/1‑150 (MQ=255)
                                                                                                                          tGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGaa                                                    >  2:1473218/1‑125 (MQ=255)
                                                                                                                          tGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGaa                                                    <  1:1473218/125‑1 (MQ=255)
                                                                                                                              gTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTATCATCAGCTTCATTATCAcc                       <  2:25294/150‑1 (MQ=255)
                                                                                                                                 cAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTATCATCAGCTTCATTATCACCCGc                    >  1:846468/1‑150 (MQ=255)
                                                                                                                                 cAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACATGAACCAAGTCTATCATCAGCTTCATTATCACCCGc                    >  1:422867/1‑150 (MQ=255)
                                                                                                                                  aaGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTATCATCAGCTTCATTATCACCCGCt                   >  1:1760043/1‑150 (MQ=255)
                                                                                                                                       aaTGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTATCATCAGCTTCATTATCACCCGCTACaaa              <  2:1760043/150‑1 (MQ=255)
                                                                                                                                          ggACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTATCATCAGCTTCATTATCACCCGCTACaaaaaa           <  1:57432/150‑1 (MQ=255)
                                                                                                                                                   tGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGTATAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTATCATCAGCTTCATTATCACCCGCTACAAAAAACAAATTAAc  <  1:1040553/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CATTGCCGAGAACAGTGTTATTAAAAAAGAACTGAAAACCCTTTCTGACACAGGTAAAGCCCTGAACCAATTCACCGACTTAATGGAGAACAAACTTATCAGCATAGACAAACAGGGACAACTGAAGTACAAGCTAATGGACGACTTTGAGAGCTTACGATTTCAAATCCTGGAGGACGAAGTGATGAAAAATGACAAGACATTGAACAGTCTTTTGTCAGCATACCCTCGAGCCAACGAACCTGAACCAAGTCTATCATCAGCTTCATTATCACCCGCTACAAAAAACAAATTAAC  >  NC_002947/4535199‑4535495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑