Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,610,345 C→G 5.5% A262A (GCC→GCG)  PP_4081 → type IV/VI secretion system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,610,3450CG 5.5% 285.9 / 4.5 91A262A (GCC→GCG) PP_4081type IV/VI secretion system protein
Reads supporting (aligned to +/- strand):  ref base C (38/48);  new base G (3/2);  total (41/50)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TGATTCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGCCTCGCTGAC  >  NC_002947/4610197‑4610494
                                                                                                                                                    |                                                                                                                                                     
tGATTCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCcgccatcgcca                                                                                                                                                      >  2:1498176/1‑150 (MQ=255)
  aTTCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATg                                                                                                                                                    >  1:1311550/1‑150 (MQ=255)
  aTTCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATg                                                                                                                                                    <  1:1437060/150‑1 (MQ=255)
  aTTCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCGGCGACCCCTACACCATCGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATATCTGCTATCGCCATg                                                                                                                                                    >  1:1570903/1‑150 (MQ=18)
    tCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGc                                                                                                                                                  <  2:1423086/150‑1 (MQ=255)
    tCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGc                                                                                                                                                  >  1:1423086/1‑150 (MQ=255)
     cACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCg                                                                                                                                                 >  2:925218/1‑150 (MQ=255)
        cAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTg                                                                                                                                              <  2:1459058/150‑1 (MQ=255)
        cAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTg                                                                                                                                              <  2:384344/150‑1 (MQ=255)
               aCGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACg                                                                                                                                       >  1:455147/1‑150 (MQ=255)
                  ggATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGa                                                                                                                                    >  1:1610176/1‑150 (MQ=255)
                      catcCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTa                                                                                                                                <  1:829309/150‑1 (MQ=255)
                      catcCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTa                                                                                                                                <  1:1498176/150‑1 (MQ=255)
                        tcCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGAAGGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTGATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACa                                                                                                                              >  2:64756/1‑150 (MQ=255)
                            cgAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTa                                                                                                                          >  2:1270089/1‑150 (MQ=255)
                                       gCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCAcc                                                                                                               >  1:1267126/1‑150 (MQ=255)
                                        ccccACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCt                                                                                                              >  2:1631727/1‑150 (MQ=255)
                                         cccACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCCTCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTg                                                                                                             >  2:666346/1‑150 (MQ=255)
                                          ccACCCCCGGAGAAGTCTGCGACCCCTGCACCAACGTCGCCCCCAGAAATTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATTTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGa                                                                                                            <  2:1009634/150‑1 (MQ=255)
                                          ccACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGa                                                                                                            <  1:925218/150‑1 (MQ=255)
                                          ccACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGa                                                                                                            >  1:1423198/1‑150 (MQ=255)
                                          ccACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGa                                                                                                            <  2:1311550/150‑1 (MQ=255)
                                          ccACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGa                                                                                                            >  2:1922365/1‑150 (MQ=255)
                                           cACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGAt                                                                                                           >  2:1238365/1‑150 (MQ=255)
                                              ccccGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGTGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATcac                                                                                                        >  2:1089495/1‑150 (MQ=255)
                                                     gaagTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAg                                                                                                 <  1:1257664/150‑1 (MQ=255)
                                                         tCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAAc                                                                                                     <  1:856952/142‑1 (MQ=255)
                                                         tCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAAc                                                                                                     >  2:856952/1‑142 (MQ=255)
                                                         tCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGc                                                                                             <  1:1557718/150‑1 (MQ=255)
                                                         tCTGCGACACCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGc                                                                                             <  2:1043189/150‑1 (MQ=255)
                                                          cTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCt                                                                                            <  2:1806069/150‑1 (MQ=255)
                                                           tGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAg                                                                                                 <  1:484416/144‑1 (MQ=255)
                                                           tGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAg                                                                                                 >  2:484416/1‑144 (MQ=255)
                                                             cGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGa                                                                                         <  2:531485/150‑1 (MQ=255)
                                                               aCCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGt                                                                                       <  1:1661129/150‑1 (MQ=255)
                                                                 cccTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCg                                                                                     >  1:449222/1‑150 (MQ=255)
                                                                  ccTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGc                                                                                    <  1:443419/150‑1 (MQ=255)
                                                                  ccTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGc                                                                                    >  1:1766375/1‑150 (MQ=255)
                                                                   cTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGCGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCt                                                                                   >  1:15847/1‑150 (MQ=255)
                                                                      cacCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGaa                                                                                <  2:1610176/150‑1 (MQ=255)
                                                                         cAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGCTATGGCCGCGGCGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGACTTACAGCTACCGCCTACACCTGATCACCACCGAGGCGCTGGAGTCGCAGAAccg                                                                             >  2:767298/1‑148 (MQ=17)
                                                                         cAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACgg                                                                             >  2:1740422/1‑150 (MQ=255)
                                                                          aaCGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGc                                                                            >  1:1697080/1‑150 (MQ=255)
                                                                             gTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCAtt                                                                         <  2:839768/150‑1 (MQ=255)
                                                                               cGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCAtttt                                                                       <  1:1334105/150‑1 (MQ=255)
                                                                                  ccccAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgca                                                                    <  1:660743/150‑1 (MQ=255)
                                                                                  ccccAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgca                                                                    <  2:1697080/150‑1 (MQ=255)
                                                                                  ccccAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgca                                                                    <  1:540469/150‑1 (MQ=255)
                                                                                  ccccAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgca                                                                    >  2:1061637/1‑150 (MQ=255)
                                                                                  ccccAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGACATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgca                                                                    <  2:1001051/150‑1 (MQ=255)
                                                                                   cccAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAAc                                                                                                     >  2:1237769/1‑116 (MQ=255)
                                                                                   cccAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAAc                                                                                                     <  1:1237769/116‑1 (MQ=255)
                                                                                   cccAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgcag                                                                   >  2:1573860/1‑150 (MQ=255)
                                                                                   cccAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgcag                                                                   >  2:218301/1‑150 (MQ=255)
                                                                                    ccAGAAACTTCCGCATGAGCCGGATATGGCCGTGATGGCACCGGTTTGTCATCTCCGCCATCGCCATGGCGGTGGGTTAACGTGTTCATATTTATGTCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATGTTgcagc                                                                  >  1:896625/1‑150 (MQ=12)
                                                                                      aGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCaa                                                                <  2:1744171/150‑1 (MQ=255)
                                                                                          aCTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGc                                                            >  1:622515/1‑150 (MQ=255)
                                                                                          aCTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGc                                                            <  1:1061637/150‑1 (MQ=255)
                                                                                            ttCCGCATGAGCCGGATATTGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCa                                                          >  1:912996/1‑150 (MQ=255)
                                                                                            ttCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCa                                                          >  1:759317/1‑150 (MQ=255)
                                                                                             tCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCaa                                                         <  1:1573860/150‑1 (MQ=255)
                                                                                                   tGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCgtgt                                                   <  2:1847185/150‑1 (MQ=255)
                                                                                                    gAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTc                                                  <  2:33513/150‑1 (MQ=255)
                                                                                                       ccGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACaa                                                                                                      <  1:1824376/95‑1 (MQ=255)
                                                                                                       ccGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACaa                                                                                                      >  2:1824376/1‑95 (MQ=255)
                                                                                                       ccGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTcaca                                               <  1:1403535/150‑1 (MQ=255)
                                                                                                        cGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCCATAGCCAACCGTGTCACAg                                              <  2:1423198/150‑1 (MQ=255)
                                                                                                           atatGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGcac                                           <  1:570242/150‑1 (MQ=255)
                                                                                                             atGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGcacca                                         <  1:1641424/150‑1 (MQ=255)
                                                                                                                   gtggtgGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGt                                   <  2:1766375/150‑1 (MQ=255)
                                                                                                                    gggtggAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGtt                                  <  1:210357/149‑1 (MQ=255)
                                                                                                                        ggAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAg                                                                                        >  2:784571/1‑92 (MQ=255)
                                                                                                                        ggAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAg                                                                                        <  1:784571/92‑1 (MQ=255)
                                                                                                                        ggAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAAc                              <  2:1291921/150‑1 (MQ=255)
                                                                                                                        ggAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAAc                              <  1:395183/150‑1 (MQ=255)
                                                                                                                        ggAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAAc                              <  1:1238365/150‑1 (MQ=255)
                                                                                                                        ggAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAAc                              <  2:896625/150‑1 (MQ=255)
                                                                                                                           gCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGaa                           >  2:716809/1‑150 (MQ=255)
                                                                                                                            cccGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAAt                          <  2:1570903/150‑1 (MQ=255)
                                                                                                                               gTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgca                                                                    >  1:878702/1‑105 (MQ=255)
                                                                                                                               gTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTgca                                                                    <  2:878702/105‑1 (MQ=255)
                                                                                                                                   gTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAg                   >  2:1182483/1‑150 (MQ=37)
                                                                                                                                      gccaccGCCATGGCGATGGCGGAGGCCTACGGGATTTACAGATACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGGCGCTGAACGGCATTTTGCAGCACTAGACAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGAtt                >  2:894685/5‑150 (MQ=11)
                                                                                                                                      aTCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGtt                <  2:449222/150‑1 (MQ=34)
                                                                                                                                      aTCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGtt                <  1:1543828/150‑1 (MQ=34)
                                                                                                                                       tctcCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGttt               >  1:589188/1‑150 (MQ=34)
                                                                                                                                        caccGCCATGGCGATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGtttt              >  1:1274023/3‑150 (MQ=12)
                                                                                                                                         accGCCATGGCGATGGCGGAGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCAttt                                                                        >  1:1464787/2‑91 (MQ=11)
                                                                                                                                         accGCCATGGCGATGGCGGAGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCAttt                                                                        <  2:1464787/90‑1 (MQ=11)
                                                                                                                                         accGCCATGGCGATGGCGGAAGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGttttt             <  1:894685/149‑1 (MQ=255)
                                                                                                                                          ccGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTg            <  1:716809/150‑1 (MQ=32)
                                                                                                                                          ccGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTg            >  2:1526015/1‑150 (MQ=32)
                                                                                                                                            gccatcgccatGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGcc          >  1:1757990/1‑150 (MQ=32)
                                                                                                                                            gccatcgccatGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGcc          >  1:1110650/1‑150 (MQ=32)
                                                                                                                                               atcgccatGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGCCTCg       >  1:1068738/1‑150 (MQ=32)
                                                                                                                                                tcgccatGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGGTCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACTGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGCCTCGc      >  2:978346/1‑150 (MQ=17)
                                                                                                                                                    catGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGCCTCGCTGAc  <  1:103884/150‑1 (MQ=32)
                                                                                                                                                    |                                                                                                                                                     
TGATTCACCAACTGCACGGGATCATCCGCGAACTGCGCGGCCCCACCCCCGAAGAAGTCTGCGACCCCTACACCAACGTCGCCCCCAGAAACTTCCGCATGAGCCGGATATGGCCGTGGTGGAGCCCGTTGGTCATCTCCGCCATCGCCATGGCGGTGGCCTACGGGATTTACAGCTACCGCCTGCACCTGATCACAACCGAGGTGCTGGAGTCGCTGAACGGCATTTTGCAGCAATAGCCAACCGTGTCACAGCACCACTGAGTTCAACGAATTCGCGAGGTTTTTGCCTCGCTGAC  >  NC_002947/4610197‑4610494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑