Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,619,711 C→T 7.3% intergenic (+934/‑1326) PP_5652 → / → PP_5654 hypothetical protein/phosphotriesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,619,7110CT 7.3% 152.7 / 2.7 55intergenic (+934/‑1326)PP_5652/PP_5654hypothetical protein/phosphotriesterase
Reads supporting (aligned to +/- strand):  ref base C (22/28);  new base T (2/2);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

AATTATTAGAGTATATAAAAAAAATTCGAATACCCGCTCCCCAACAATACACGAGAGAAATTGAAAAAATATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTCTTGGTGCACTGCGTGGTAGGCACGTCGG  >  NC_002947/4619562‑4619859
                                                                                                                                                     |                                                                                                                                                    
aaTTATTAGAGTATATAAAAAAAATTCGAATACCCGCTCCCCAACAATACACGAGAGAAATTGAAAAAATATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCAc                                                                                                                                                      <  2:568293/150‑1 (MQ=255)
               taAAAAAAATTCGAATACCCGCTCCCCAACAATACACGAGAGAAATTGAAAAAATATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGAcc                                                                                                                                       <  1:267825/150‑1 (MQ=255)
               taAAAAAAATTCGAATACCCGCTCCCCAACAATACACGAGAGAAATTGAAAAAATATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGAcc                                                                                                                                       <  1:598369/150‑1 (MQ=255)
                     aaaTTCGAATACCCGCTCCCCAACAATACACGAGAGAAATTGAAAAAATATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAAc                                                                                                                                 <  1:550014/150‑1 (MQ=255)
                        cgtgattACCCGCTCCCCAACAATACACGAGAGAAATTGAAAAAATATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCaa                                                                                                                              >  2:1604122/7‑150 (MQ=255)
                                       cccAACAATACACGAGAGAAATTGAAAAAATATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCAGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGcc                                                                                                               >  1:994226/1‑150 (MQ=255)
                                                 cacGAGAGAAATTGAAAAAATATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGc                                                                                                     <  1:155456/150‑1 (MQ=255)
                                                  acGAGAGAAATTGAAAAAATATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCa                                                                                                    >  2:102976/1‑150 (MQ=255)
                                                   cGAGAGAAATTGAAAAAATATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCAc                                                                                                   <  1:1212480/150‑1 (MQ=255)
                                                   cGAGAGAAATTGAAAAAATATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCAc                                                                                                   <  2:1923851/150‑1 (MQ=255)
                                                            ttGAAAAAATATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAg                                                                                          >  1:308477/1‑150 (MQ=255)
                                                               aaaaaaTATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACCTAAATGCACCAAGCTAAGaaa                                                                                       >  2:642556/1‑150 (MQ=255)
                                                                 aaaaTATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAgg                                                                                     <  1:1750817/150‑1 (MQ=255)
                                                                 aaaaTATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACAAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAgg                                                                                     <  1:520599/150‑1 (MQ=255)
                                                                       ttCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCg                                                                               <  1:1604122/150‑1 (MQ=255)
                                                                          tctGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCggtg                                                                            <  1:1246725/150‑1 (MQ=255)
                                                                          tctGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACACAAATGCACCAAGCCAAGAAAGGCAATCggta                                                                            >  2:396553/1‑149 (MQ=255)
                                                                              aaaCCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCACGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTAc                                                                        >  2:1814256/1‑150 (MQ=255)
                                                                                 ccAAGCCCTCGTGAGTAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTc                                                                     <  1:1405529/150‑1 (MQ=255)
                                                                                 ccAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCAGCGGTACCTc                                                                     >  2:663043/1‑150 (MQ=255)
                                                                                   aaGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCATGCGCCGAGTTCGACCGAGAACCAAGACAGCAAgcgc                                                                                                                  >  2:1396597/1‑103 (MQ=255)
                                                                                   aaGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCATGCGCCGAGTTCGACCGAGAACCAAGACAGCAAgcgc                                                                                                                  >  2:1396347/1‑103 (MQ=255)
                                                                                   aaGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCATGCGCCGAGTTCGACCGAGAACCAAGACAGCAAgcgc                                                                                                                  <  1:1396597/103‑1 (MQ=255)
                                                                                   aaGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCATGCGCCGAGTTCGACCGAGAACCAAGACAGCAAgcgc                                                                                                                  <  1:1396347/103‑1 (MQ=255)
                                                                                            tGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGc                                                          <  1:1721019/150‑1 (MQ=255)
                                                                                            tGAGCAAAAGCGTCATTCGACGCTGGATGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGc                                                          <  2:408757/150‑1 (MQ=255)
                                                                                                    aTGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAATGCTCACAttt                                                  <  2:840662/150‑2 (MQ=255)
                                                                                                        tCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGgtgt                                              >  2:1008584/1‑150 (MQ=255)
                                                                                                          aTTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTgg                                            >  2:352213/1‑150 (MQ=255)
                                                                                                           ttCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGAGCACGATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGa                                           >  1:595192/1‑150 (MQ=255)
                                                                                                              ggCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGgt                                                <  1:722492/142‑1 (MQ=255)
                                                                                                              ggCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGgt                                                >  2:722492/1‑142 (MQ=255)
                                                                                                              ggCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGc                                        <  2:1391327/150‑1 (MQ=255)
                                                                                                                    ggaggaAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTc                                  >  1:1845956/1‑150 (MQ=255)
                                                                                                                    ggaggaAACGCTGTACGAGGCCACGCAGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTc                                  <  2:994226/150‑1 (MQ=255)
                                                                                                                        gaAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTc                              >  2:514823/1‑150 (MQ=255)
                                                                                                                          aaCGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCTGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTCtt                            <  2:121865/150‑1 (MQ=255)
                                                                                                                           aCGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTCTTg                           <  1:102976/150‑1 (MQ=255)
                                                                                                                           aCGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTCTTg                           <  2:308477/150‑1 (MQ=255)
                                                                                                                             gCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCACTCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTCTCggg                         >  1:739896/1‑149 (MQ=255)
                                                                                                                                gTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCAAATTGGTGTGGATGCGGAGTCGGTCTTGGTGCa                      <  1:252032/150‑1 (MQ=255)
                                                                                                                                    gAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTca                                                       <  2:208169/113‑1 (MQ=255)
                                                                                                                                    gAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTca                                                       >  1:208169/1‑113 (MQ=255)
                                                                                                                                    gAGGCCACGCGGATCAACGCGCCGAGTTCGACTGAGAACCAAGACAGCAAGCGCGACACATAAATGCACCAAGCTAAAAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTAACATTGGTGTGAATGCGGAATCGGTCTTGGTGCACTGc                  <  2:863027/150‑1 (MQ=255)
                                                                                                                                       gCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGgtgt                                              <  2:1637357/119‑1 (MQ=255)
                                                                                                                                       gCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGgtgt                                              >  1:1637357/1‑119 (MQ=255)
                                                                                                                                       gCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTCTTGGTGCACTGCGTg               <  1:1065763/150‑1 (MQ=255)
                                                                                                                                        ccACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTCTTGGTGCACTGCGTgg              >  1:985175/1‑150 (MQ=255)
                                                                                                                                         cACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTCTTGGTGCACTGCGTGGt             >  2:1439790/1‑150 (MQ=255)
                                                                                                                                         cACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTCTTGGTGCACTGCGTGGt             >  1:1181007/1‑150 (MQ=255)
                                                                                                                                          aCGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCgg                                                                   <  2:1393488/95‑1 (MQ=255)
                                                                                                                                          aCGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCgg                                                                   >  1:1393488/1‑95 (MQ=255)
                                                                                                                                           cgcgGGTCCAAGCGGCGAGTCCGACCGTGAACCAAGGCAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTTGGTATGGCGGGTCACATTGGTGTGGATGCGGAGTCGGTCTTGGGGCACTGGGTGGGAg           <  2:739896/150‑1 (MQ=255)
                                                                                                                                            gcgGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAACAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTCTTGGTGCACTGCGTGGTAgg          >  2:899486/1‑150 (MQ=255)
                                                                                                                                                  ccACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTCTTGGTGCACTGCGTGGTAGGCACGTc    <  1:1428956/150‑1 (MQ=255)
                                                                                                                                                  ccACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTCTTGGTGCACTGCGTGGTAGGCACGTc    <  1:1439419/150‑1 (MQ=255)
                                                                                                                                                    ccgcgCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCGCCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGACGGTCTTGGTGCACTGCGTGGTAGGCACGTAgg  >  2:1151256/2‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AATTATTAGAGTATATAAAAAAAATTCGAATACCCGCTCCCCAACAATACACGAGAGAAATTGAAAAAATATTCTCTGAAACCAAGCCCTCGTGAGCAAAATGGTCATTCGGCGATGGAGGAAACGCTGTACGAGGCCACGCGGATCCACGCGCCGAGTTCGACCGAGAACCAAGACAGCAAGCGCGCCACATAAATGCACCAAGCTAAGAAAGGCAATCGGTGGTACTTCGGTATGAAGGCTCACATTGGTGTGGATGCGGAGTCGGTCTTGGTGCACTGCGTGGTAGGCACGTCGG  >  NC_002947/4619562‑4619859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑